FastQCFastQC Report
Mon 16 Jan 2017
ERR1146657_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146657_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4422502
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC57050.041.79414794
GTACTAG63450.036.6097031
TAGGCAT76400.033.4623075
CAACGCA77550.032.905495
GCATCGT65900.032.5890248
TACCCCG3550.031.7711735
GTATCAA98300.030.518841
TAGTACT83950.030.2863274
CTGTGCG84150.029.7651969
CTAACGC33250.029.6823083
ATCAACG86400.029.645093
CTAGGCA88500.029.5257914
TCTAACG34500.029.1520842
TAACGCC34200.029.1326354
ACTGTGC91000.028.4028478
CGCCTAC35000.028.3305457
GTACTGT90000.027.9877366
TACTGTG99200.026.8603657
CTAGTAC99650.026.8352093
AACGCAG105250.026.6561666
Produced by FastQC (version 0.11.5)