##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146657_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4422502
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.400698970854055	31.0	31.0	34.0	28.0	34.0
2	31.529812762097112	33.0	31.0	34.0	28.0	34.0
3	31.65420207837102	33.0	31.0	34.0	28.0	34.0
4	35.099481243875076	37.0	35.0	37.0	32.0	37.0
5	35.25120259979532	37.0	35.0	37.0	32.0	37.0
6	35.353788195008164	37.0	35.0	37.0	32.0	37.0
7	35.30456877125211	37.0	35.0	37.0	32.0	37.0
8	35.28629563084426	37.0	35.0	37.0	32.0	37.0
9	36.833719238566765	39.0	37.0	39.0	32.0	39.0
10-11	36.67962840943882	39.0	37.0	39.0	32.0	39.0
12-13	36.607756989143255	39.0	37.0	39.0	32.0	39.0
14-15	37.798732029968555	40.0	38.0	41.0	32.0	41.0
16-17	37.801670072732584	40.0	38.0	41.0	32.0	41.0
18-19	37.74793080930206	40.0	37.5	41.0	32.0	41.0
20-21	37.794599753714074	40.0	37.5	41.0	32.0	41.0
22-23	37.71150256121987	40.0	37.0	41.0	31.5	41.0
24-25	37.6380210794704	40.0	37.0	41.0	31.0	41.0
26-27	37.65522638542617	40.0	37.0	41.0	31.0	41.0
28-29	37.61467253152175	40.0	37.0	41.0	31.0	41.0
30-31	37.460934443896235	40.0	37.0	41.0	31.0	41.0
32-33	37.4465932406588	40.0	37.0	41.0	31.0	41.0
34-35	37.371789882740586	40.0	36.5	41.0	30.5	41.0
36-37	37.252061728858465	40.0	36.0	41.0	30.0	41.0
38-39	37.1488050203256	40.0	36.0	41.0	30.0	41.0
40-41	37.05776074267462	40.0	36.0	41.0	30.0	41.0
42-43	36.878195984987684	39.0	35.5	41.0	30.0	41.0
44-45	36.70820194089228	39.0	35.0	41.0	30.0	41.0
46-47	36.68679561931232	39.0	35.0	41.0	30.0	41.0
48-49	36.50731486384856	39.0	35.0	41.0	29.5	41.0
50-51	36.2911913889468	39.0	35.0	41.0	29.0	41.0
52-53	36.039347184014844	38.0	35.0	40.0	29.0	41.0
54-55	35.77712344731557	38.0	34.0	40.0	28.0	41.0
56-57	35.49291520953524	37.0	34.0	40.0	28.0	41.0
58-59	35.14081463388824	37.0	34.0	40.0	28.0	41.0
60-61	34.851374063821794	36.0	33.5	39.5	27.0	41.0
62-63	34.493482309335306	35.5	33.0	39.0	26.5	41.0
64-65	34.314842141394166	35.0	33.0	39.0	26.5	41.0
66-67	34.03489370948843	35.0	33.0	38.0	26.0	40.5
68-69	33.88444651918755	35.0	33.0	37.0	26.0	40.0
70-71	33.805068149206036	35.0	33.0	37.0	26.5	40.0
72-73	33.57649369067555	35.0	33.0	36.5	26.0	39.0
74-75	33.33701827607992	35.0	33.0	36.0	26.0	39.0
76-77	33.09521273252109	35.0	33.0	36.0	26.0	39.0
78-79	32.86679022417627	35.0	33.0	35.0	26.0	37.5
80-81	32.71405756289087	35.0	33.0	35.0	26.0	37.0
82-83	32.48712572656835	35.0	33.0	35.0	25.0	36.5
84-85	32.34873505992762	35.0	32.0	35.0	25.0	36.0
86-87	32.21082082043151	35.0	32.0	35.0	25.0	36.0
88-89	32.121161166235765	35.0	32.0	35.0	25.0	36.0
90-91	31.98245258001014	35.0	32.0	35.0	25.0	35.5
92-93	31.83650793148313	35.0	32.0	35.0	24.0	35.0
94-95	31.781554423265383	35.0	32.0	35.0	24.0	35.0
96-97	31.673749497456416	35.0	32.0	35.0	24.0	35.0
98-99	31.57124089485997	35.0	32.0	35.0	24.0	35.0
100	30.575137331763784	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2802857522622588
1101	2	0.3997097168922359
1101	3	0.4259353348294681
1101	4	0.4972958819349387
1101	5	0.36856981702830893
1101	6	0.358175537224092
1101	7	0.38966581790116095
1101	8	0.36918008109052636
1101	9	0.4964563205281536
1101	10-11	0.5658393878587162
1101	12-13	0.4572819222549924
1101	14-15	0.6916884140376496
1101	16-17	0.6663628934357462
1101	18-19	0.6547721974746494
1101	20-21	0.5841691240403222
1101	22-23	0.6219474068267132
1101	24-25	0.6972255112598162
1101	26-27	0.6911243087790169
1101	28-29	0.6459635162704274
1101	30-31	0.7524357260075263
1101	32-33	0.6919468241772577
1101	34-35	0.6896706483153352
1101	36-37	0.7438311850623833
1101	38-39	0.6844789525869146
1101	40-41	0.6725243776930938
1101	42-43	0.6077061807410189
1101	44-45	0.6452513580374557
1101	46-47	0.609251139722808
1101	48-49	0.6030417702129398
1101	50-51	0.6356309764763992
1101	52-53	0.6453549164657488
1101	54-55	0.6075597582951175
1101	56-57	0.5748777280057951
1101	58-59	0.4914784137590189
1101	60-61	0.5303126590233234
1101	62-63	0.56791405307969
1101	64-65	0.5858515713778232
1101	66-67	0.6295951571386667
1101	68-69	0.5577614132715496
1101	70-71	0.5669278909675484
1101	72-73	0.5751929094733583
1101	74-75	0.5195490577208091
1101	76-77	0.5882437084954546
1101	78-79	0.5710267099518305
1101	80-81	0.5820192597038769
1101	82-83	0.46833292391556114
1101	84-85	0.47351185965125353
1101	86-87	0.42405000544179217
1101	88-89	0.5198882724611451
1101	90-91	0.5195285697955825
1101	92-93	0.5669708319957749
1101	94-95	0.6008289219011154
1101	96-97	0.5578247937310508
1101	98-99	0.48979064149991913
1101	100	0.5395478413141355
1102	1	0.3311876528551423
1102	2	0.4397824514373596
1102	3	0.4235640838088841
1102	4	0.4929810302825359
1102	5	0.3914891595437453
1102	6	0.41959647238530096
1102	7	0.37499784643966905
1102	8	0.4583758653605656
1102	9	0.5825038018566744
1102	10-11	0.6940775653399456
1102	12-13	0.6024064651156564
1102	14-15	0.7812455895840102
1102	16-17	0.7648895383364192
1102	18-19	0.7666039876745145
1102	20-21	0.7195371703527726
1102	22-23	0.8012855826575347
1102	24-25	0.7896787925178614
1102	26-27	0.6896214890047716
1102	28-29	0.7181678671398473
1102	30-31	0.7870436371028404
1102	32-33	0.6896428273693402
1102	34-35	0.7558500313920291
1102	36-37	0.7772698291242506
1102	38-39	0.7799106658970842
1102	40-41	0.6977245141917194
1102	42-43	0.7194624889483094
1102	44-45	0.776212493791455
1102	46-47	0.6401062123554198
1102	48-49	0.5959092558713621
1102	50-51	0.6097118922016023
1102	52-53	0.5895425360598097
1102	54-55	0.5078179018555531
1102	56-57	0.5246651406190068
1102	58-59	0.5012163189225731
1102	60-61	0.5829713969255721
1102	62-63	0.5656176530125023
1102	64-65	0.5509241845692543
1102	66-67	0.5539592395543238
1102	68-69	0.44625111325611044
1102	70-71	0.4162877502749467
1102	72-73	0.3791886737443093
1102	74-75	0.4197251419189101
1102	76-77	0.39988875964360915
1102	78-79	0.40022848423979696
1102	80-81	0.441841602762004
1102	82-83	0.49295951862433895
1102	84-85	0.4670941670605231
1102	86-87	0.4701138743720463
1102	88-89	0.5017691477998341
1102	90-91	0.47198379756673603
1102	92-93	0.44155405489725297
1102	94-95	0.521536083807316
1102	96-97	0.4897091397851554
1102	98-99	0.4988562472441238
1102	100	0.5759968091767647
1103	1	0.23979188804120355
1103	2	0.2998168747456411
1103	3	0.354564549797054
1103	4	0.3991630435250926
1103	5	0.2672396368680694
1103	6	0.2992201025199748
1103	7	0.27659928280237267
1103	8	0.2682562134913553
1103	9	0.35183005821577495
1103	10-11	0.5038619179781776
1103	12-13	0.40435946688024416
1103	14-15	0.5876908427274969
1103	16-17	0.5153400176867748
1103	18-19	0.5845625253399618
1103	20-21	0.4849001153208192
1103	22-23	0.5402050979140611
1103	24-25	0.5596815759047473
1103	26-27	0.5135799620300432
1103	28-29	0.49451561491193985
1103	30-31	0.49137628994551363
1103	32-33	0.5308329736973505
1103	34-35	0.5030228676584514
1103	36-37	0.5287378606956707
1103	38-39	0.5187494774053576
1103	40-41	0.45924221257678255
1103	42-43	0.45944904957935506
1103	44-45	0.42725235652272175
1103	46-47	0.39862962319585904
1103	48-49	0.4491148540969476
1103	50-51	0.392795484497654
1103	52-53	0.3464586736256905
1103	54-55	0.3756333628220503
1103	56-57	0.3825909192196306
1103	58-59	0.3876854889330019
1103	60-61	0.40313037800336105
1103	62-63	0.3486747380104873
1103	64-65	0.3102770881193564
1103	66-67	0.3611916557367465
1103	68-69	0.3602751864172191
1103	70-71	0.3256259650675162
1103	72-73	0.35862689750932475
1103	74-75	0.30342956938539345
1103	76-77	0.2893303649583814
1103	78-79	0.37985086016200853
1103	80-81	0.2540033392481078
1103	82-83	0.3578272426242819
1103	84-85	0.3135115332722336
1103	86-87	0.3323849926568201
1103	88-89	0.4723087100025083
1103	90-91	0.4664279740170656
1103	92-93	0.38456522124730697
1103	94-95	0.2971170494930284
1103	96-97	0.37704306517109387
1103	98-99	0.41439589145298683
1103	100	0.4789910484824347
1104	1	0.17295349487519474
1104	2	0.25485426480780404
1104	3	0.22877422194429187
1104	4	0.3869737021943749
1104	5	0.2805552543544394
1104	6	0.2442653355848563
1104	7	0.24408827353276052
1104	8	0.28657243611827
1104	9	0.3491236028432212
1104	10-11	0.4757592384920599
1104	12-13	0.3419442937671562
1104	14-15	0.48605026857777034
1104	16-17	0.4804993602468386
1104	18-19	0.4189498753294316
1104	20-21	0.41608970251966326
1104	22-23	0.44630219867138976
1104	24-25	0.4763897685548173
1104	26-27	0.44025772611671243
1104	28-29	0.4050544711295885
1104	30-31	0.35153483676957364
1104	32-33	0.41443287859021893
1104	34-35	0.48427430308042574
1104	36-37	0.45699261152547876
1104	38-39	0.45392624991919917
1104	40-41	0.4392067951775118
1104	42-43	0.4811644705618221
1104	44-45	0.4307672224912338
1104	46-47	0.4008086294883739
1104	48-49	0.3953381099818216
1104	50-51	0.33601726707970414
1104	52-53	0.383324312411105
1104	54-55	0.4354894823177773
1104	56-57	0.4403109402232488
1104	58-59	0.417643686586203
1104	60-61	0.41175970606864354
1104	62-63	0.47533139365121
1104	64-65	0.45260921879540206
1104	66-67	0.4147120727977125
1104	68-69	0.408022919999091
1104	70-71	0.3714406081346482
1104	72-73	0.35768087699934625
1104	74-75	0.4580854111835464
1104	76-77	0.37339284500542647
1104	78-79	0.3584441799821221
1104	80-81	0.4213984617199813
1104	82-83	0.37653428121890187
1104	84-85	0.40841667868227205
1104	86-87	0.35554243498025073
1104	88-89	0.335441665575658
1104	90-91	0.3980853777449198
1104	92-93	0.37262055682395356
1104	94-95	0.4423155689475813
1104	96-97	0.4517022566842641
1104	98-99	0.34373510656186923
1104	100	0.46969361189592007
1105	1	0.2025798459683763
1105	2	0.3305551879499973
1105	3	0.288032227201132
1105	4	0.3726578594752823
1105	5	0.2509272035591863
1105	6	0.24449635909105183
1105	7	0.2837820280114798
1105	8	0.28624269391548296
1105	9	0.41240419102172154
1105	10-11	0.42327733352036745
1105	12-13	0.3081445961781455
1105	14-15	0.4489400952489433
1105	16-17	0.4281339868494811
1105	18-19	0.4638280950750726
1105	20-21	0.39332861808254904
1105	22-23	0.4202445420574392
1105	24-25	0.40300095014691095
1105	26-27	0.4516568460789685
1105	28-29	0.47610735498010825
1105	30-31	0.4964037081537995
1105	32-33	0.46870283615205466
1105	34-35	0.5507325522785038
1105	36-37	0.5306344135904126
1105	38-39	0.4930531197614556
1105	40-41	0.48385800271385904
1105	42-43	0.5486808182963898
1105	44-45	0.5287610981247184
1105	46-47	0.533099807490693
1105	48-49	0.43343421249696945
1105	50-51	0.430989242391874
1105	52-53	0.47621071928170267
1105	54-55	0.41862456240094303
1105	56-57	0.48777532466594664
1105	58-59	0.5238381148268161
1105	60-61	0.5605541753740582
1105	62-63	0.4582347363532975
1105	64-65	0.397013697578366
1105	66-67	0.3953820069716443
1105	68-69	0.35272889193633006
1105	70-71	0.3568183771193034
1105	72-73	0.3505237035087134
1105	74-75	0.3292598577265551
1105	76-77	0.34928885995192616
1105	78-79	0.4221673025931878
1105	80-81	0.3739690911400544
1105	82-83	0.41536791845896914
1105	84-85	0.3647876379805197
1105	86-87	0.3823020685516809
1105	88-89	0.31732076780662055
1105	90-91	0.3497677088659259
1105	92-93	0.38290892147939104
1105	94-95	0.3296650263346983
1105	96-97	0.3210348040753601
1105	98-99	0.3210915395263001
1105	100	0.3301320262506522
1106	1	0.1344924269624883
1106	2	0.230809846994525
1106	3	0.18416858481921494
1106	4	0.2940663809572115
1106	5	0.16717756715969045
1106	6	0.23098925307212426
1106	7	0.2558043013064051
1106	8	0.22844969210136412
1106	9	0.3161990516763993
1106	10-11	0.3908110510871481
1106	12-13	0.2635857318870052
1106	14-15	0.39879794881659336
1106	16-17	0.282334917761105
1106	18-19	0.3733645813848838
1106	20-21	0.35852895867607515
1106	22-23	0.3700475256669975
1106	24-25	0.43089238525190865
1106	26-27	0.35603378198283764
1106	28-29	0.3251578205852823
1106	30-31	0.4666539378473544
1106	32-33	0.39136021629730067
1106	34-35	0.34707098991046337
1106	36-37	0.395846114488819
1106	38-39	0.3145458507946941
1106	40-41	0.32957002554370973
1106	42-43	0.24979720520374826
1106	44-45	0.2871630576492876
1106	46-47	0.39459011014085377
1106	48-49	0.2790000539423758
1106	50-51	0.31405945260220847
1106	52-53	0.25742547591794107
1106	54-55	0.35567577224308167
1106	56-57	0.28114049784782225
1106	58-59	0.3474505264697285
1106	60-61	0.29060759239002465
1106	62-63	0.3181386279045668
1106	64-65	0.3204049718355506
1106	66-67	0.30211136599059074
1106	68-69	0.29946046057724374
1106	70-71	0.32338969179249233
1106	72-73	0.2810215694312035
1106	74-75	0.2948668042497715
1106	76-77	0.23295461022515696
1106	78-79	0.2764323794730075
1106	80-81	0.21753954601918934
1106	82-83	0.2520545968259853
1106	84-85	0.2385219258400042
1106	86-87	0.2591248747253161
1106	88-89	0.25664079314248056
1106	90-91	0.2151767102746902
1106	92-93	0.27470645344530453
1106	94-95	0.3031522354242533
1106	96-97	0.31154949977966595
1106	98-99	0.29639863217250806
1106	100	0.24272854027215018
1107	1	0.09713701901851479
1107	2	0.10161766990302112
1107	3	0.10035240124205203
1107	4	0.18557312035591167
1107	5	0.08898629404210112
1107	6	0.1642806870231368
1107	7	0.07936720537589537
1107	8	0.12074779719520023
1107	9	0.1785046853768293
1107	10-11	0.2665263095215664
1107	12-13	0.1623915905467399
1107	14-15	0.2621413849143295
1107	16-17	0.16198720431737001
1107	18-19	0.1267909202560773
1107	20-21	0.17444767396382588
1107	22-23	0.3164500137989208
1107	24-25	0.34785250747341934
1107	26-27	0.18331529536401803
1107	28-29	0.20644175244942886
1107	30-31	0.3093108005326002
1107	32-33	0.30097387058553693
1107	34-35	0.29753999667708797
1107	36-37	0.26387466536922943
1107	38-39	0.367647917783934
1107	40-41	0.2623590045527209
1107	42-43	0.2707385222991405
1107	44-45	0.2836904937284075
1107	46-47	0.24487609053105075
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2214	96-97	-0.6149766691141636
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2214	100	-0.4120720822704129
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2215	24-25	-1.0119672818711507
2215	26-27	-0.9641332726622025
2215	28-29	-0.8473701477265365
2215	30-31	-3.7215391338663224
2215	32-33	-1.387440179008081
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2215	100	-0.5507662559813866
2216	1	-0.5071421644001255
2216	2	-0.7270947232797589
2216	3	-0.7586669362190648
2216	4	-0.5564860784053707
2216	5	-0.47711682966143343
2216	6	-0.45886321144165265
2216	7	-0.6423204362530086
2216	8	-0.6301320389655842
2216	9	-0.7798321525530341
2216	10-11	-1.1625164509344756
2216	12-13	-0.7130562872885164
2216	14-15	-0.7935050885167598
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2216	18-19	-1.169277817306444
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2216	24-25	-1.2276593537327187
2216	26-27	-1.5671506143405196
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2216	30-31	-1.4960682289756733
2216	32-33	-1.72751723181036
2216	34-35	-1.3755542536568726
2216	36-37	-1.41769099172015
2216	38-39	-1.3112336120283388
2216	40-41	-1.194506094866064
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2216	50-51	-1.2257425038092435
2216	52-53	-1.2913994075550903
2216	54-55	-1.3488877613133
2216	56-57	-1.2453244481368486
2216	58-59	-1.209272324601912
2216	60-61	-1.24242998393418
2216	62-63	-1.3064767879310821
2216	64-65	-1.2449688535961698
2216	66-67	-1.2059184283269957
2216	68-69	-1.2437657365205936
2216	70-71	-1.2156480561855005
2216	72-73	-1.128081388449175
2216	74-75	-1.1758624084249334
2216	76-77	-1.161899919498282
2216	78-79	-1.0417315694603033
2216	80-81	-1.1795445995997014
2216	82-83	-1.183828886537441
2216	84-85	-1.2104093261858644
2216	86-87	-1.239978182944597
2216	88-89	-1.2752494006535429
2216	90-91	-1.288788415370604
2216	92-93	-1.2795383037975085
2216	94-95	-1.3086387155022514
2216	96-97	-1.3511282804181306
2216	98-99	-1.363927664643878
2216	100	-0.9734579704062369
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	10.0
3	26.0
4	36.0
5	26.0
6	52.0
7	146.0
8	466.0
9	760.0
10	1067.0
11	1965.0
12	3373.0
13	5176.0
14	7458.0
15	10013.0
16	12303.0
17	14612.0
18	16826.0
19	18794.0
20	21742.0
21	24708.0
22	28530.0
23	32810.0
24	38420.0
25	44518.0
26	50625.0
27	57302.0
28	66744.0
29	80810.0
30	94717.0
31	113527.0
32	141304.0
33	182972.0
34	263480.0
35	384230.0
36	640001.0
37	1098191.0
38	767333.0
39	197309.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.427858006313656	19.725910492510877	11.553836780248071	24.292394720927398
2	16.382249318203403	19.464851859054665	39.062919762829765	25.08997905991217
3	19.341169212984582	25.75119224031277	28.092888121738426	26.814750424964217
4	12.595145286589734	15.959171000438033	39.29068692754248	32.15499678542975
5	13.567410013703151	38.609559644893885	34.27653685605358	13.546493485349384
6	30.790439222847677	37.69021902576925	17.537776981830238	13.981564769552834
7	27.70329183305219	31.260294044491076	23.008348294840427	18.028065827616306
8	27.262370998815133	34.04313003681226	19.207179746927036	19.487319217445574
9	26.564169031103845	14.375046919417992	20.677308367529864	38.383475681948305
10-11	26.793168086684393	24.916958358206255	26.565558238816227	21.724315316293122
12-13	26.64233873730381	22.664736984082946	26.704419163055015	23.98850511555823
14-15	24.075809729941913	23.856612096389977	24.07008897470273	27.99748919896538
16-17	23.19008103377361	26.61515254912575	25.25997308748016	24.934793329620483
18-19	22.386492049341626	27.664220991501846	26.786513982972043	23.16277297618449
20-21	24.541354702768498	26.815420444782518	25.776822752347762	22.866402100101222
22-23	25.09546473675362	26.124872793768244	25.582989226885722	23.196673242592418
24-25	24.547071914501615	26.017841710220996	26.365884399719068	23.06920197555832
26-27	24.384182929564826	26.43384627490991	24.876479308673062	24.305491486852198
28-29	23.202255016486827	26.89214118025886	26.02490368173433	23.880700121519983
30-31	23.960653265865766	26.65491253027572	26.148182167858025	23.23625203600049
32-33	24.376045144593174	26.580507367880724	24.990180304755402	24.053267182770703
34-35	24.329772072927167	26.70773450586028	25.909890821807146	23.052602599405407
36-37	23.914981607826373	25.928990482065693	25.261935691332145	24.89409221877579
38-39	24.174678486984806	26.243426372660224	25.951253851010442	23.630641289344528
40-41	23.73934586819133	25.127831491275437	26.901247515958023	24.231575124575215
42-43	23.71975850678747	25.349799245650324	26.230669167596528	24.699773079965677
44-45	24.195304977443108	26.105067229660772	26.121305704555876	23.578322088340244
46-47	23.898201511333873	26.239778436002613	25.32282580941941	24.539194243244104
48-49	24.696564174194986	25.9934913903067	25.606249694675686	23.703694740822627
50-51	23.998078099322097	25.47988432862783	26.19333737329008	24.328700198759993
52-53	23.786882732035124	25.897819639035614	25.847507270357795	24.467790358571467
54-55	23.452421459315506	25.86176423393891	25.85581621666976	24.829998090075826
56-57	24.852710318967546	25.426015819075136	25.288877515978452	24.43239634597886
58-59	23.276132872846897	26.04591536153168	26.383783073527585	24.294168692093834
60-61	23.45703707833113	26.48142069205241	25.67478496404317	24.386757265573294
62-63	24.044139587964327	25.86275148991802	25.080543825305067	25.01256509681259
64-65	23.109834758205867	26.01385421829095	25.838785366129997	25.037525657373195
66-67	23.97147044769058	25.807537126252665	25.664181519542296	24.55681090651446
68-69	23.302498012609178	25.60481862705581	26.252531089621574	24.84015227071344
70-71	23.025835023055336	25.46679123855445	25.983708792143783	25.523664946246434
72-73	23.757677628801332	25.33379495402751	26.582206059970137	24.32632135720102
74-75	24.011823718854018	25.497861920474612	26.11108560708048	24.379228753590887
76-77	23.678743558504188	25.52412504487493	25.441911346926315	25.355220049694566
78-79	23.83730081194911	25.59296211265975	25.240629025371604	25.32910805001954
80-81	23.188651766956657	26.15749399028948	26.151444705009713	24.50240953774415
82-83	24.08486412358016	25.014277400709172	27.354877896141478	23.54598057956919
84-85	23.95477890469192	25.386956646608656	26.226111808506865	24.432152640192566
86-87	22.52895199772233	26.188704769111055	26.684989193952774	24.597354039213844
88-89	23.997882012061407	24.61772025033686	26.327725278466456	25.05667245913528
90-91	23.44256748887407	25.06782753115928	25.973404506728702	25.516200473237948
92-93	23.69872107187047	25.033222326534116	26.49473332984329	24.77332327175213
94-95	22.721540226776362	25.13369050467261	27.201144510118823	24.943624758432204
96-97	23.02009696838125	24.916848708520465	26.664156524334814	25.39889779876347
98-99	22.342844369276087	25.407628344485904	26.564438605554614	25.685088680683393
100	23.183287615334045	24.638910929246673	25.57670422343633	26.60109723198295
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	261.0
1	173.0
2	110.5
3	149.0
4	142.5
5	124.5
6	116.5
7	108.0
8	118.0
9	117.5
10	106.5
11	108.0
12	114.0
13	122.5
14	131.0
15	171.5
16	200.5
17	215.0
18	258.0
19	326.0
20	415.0
21	501.0
22	685.5
23	977.0
24	1339.0
25	1801.5
26	2437.5
27	3232.5
28	4682.5
29	7979.5
30	13230.5
31	18893.5
32	27435.5
33	38009.0
34	47845.0
35	57082.5
36	67340.0
37	85859.5
38	98938.5
39	104445.0
40	104795.0
41	101569.5
42	105473.5
43	117699.5
44	136724.0
45	167888.0
46	199156.0
47	225058.0
48	245020.0
49	246269.0
50	236292.0
51	244854.5
52	252003.5
53	240483.0
54	226754.0
55	194621.0
56	169231.0
57	137796.5
58	105167.5
59	102470.5
60	85662.5
61	57131.0
62	42281.0
63	36273.0
64	26264.0
65	11537.0
66	5645.0
67	3764.5
68	2719.5
69	1907.5
70	1248.5
71	809.0
72	523.5
73	347.0
74	229.5
75	158.5
76	116.0
77	72.5
78	39.0
79	32.5
80	27.0
81	25.5
82	22.5
83	15.5
84	14.0
85	14.0
86	16.0
87	13.0
88	10.5
89	10.0
90	7.5
91	8.0
92	7.0
93	6.0
94	7.0
95	4.5
96	2.5
97	3.5
98	4.0
99	6.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0578631733801364
2	0.008185411787264313
3	0.008253246691578658
4	0.010152624012380321
5	0.0036630848329746377
6	0.002261163477144838
7	9.49688660400832E-4
8	0.0014019213558297994
9	5.879025040576579E-4
10-11	4.974559649718643E-4
12-13	2.9395125202882894E-4
14-15	2.0350471294303544E-4
16-17	5.652908692862095E-4
18-19	0.0015715086166156623
20-21	0.0010966642864152464
22-23	0.0013001689993582819
24-25	0.0012662515472011092
26-27	0.003753531372060431
28-29	0.004160540797946501
30-31	0.005178064362661679
32-33	0.006037306483976717
34-35	0.003267381224474291
36-37	0.008513280491450314
38-39	0.006466927544634237
40-41	0.006195587927376856
42-43	0.015375911644584898
44-45	0.020452223650775057
46-47	0.01910683138187388
48-49	0.02202373226739072
50-51	0.018937244121088017
52-53	0.025415477483107976
54-55	0.01945731172083133
56-57	0.015568110540142208
58-59	0.018111919451930154
60-61	0.016766527183028974
62-63	0.011995472246253365
64-65	0.01192763734193902
66-67	0.014267941540783927
68-69	0.005573767971162025
70-71	0.009576027325708388
72-73	0.0090672655433508
74-75	0.01227811768089647
76-77	0.01106839522062398
78-79	0.012436399124296607
80-81	0.011124924307552603
82-83	0.006862631153134583
84-85	0.011192759211866948
86-87	0.009971730934208736
88-89	0.008603727030536109
90-91	0.014132271732155238
92-93	0.009055959725965077
94-95	0.007868848900464036
96-97	0.009994342568980184
98-99	0.00968908549956563
100	0.2578178596640544
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4422502.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.39005920962087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.75118467017775	51.048768201222025
2	14.516824400279868	19.565793117410585
3	4.892807353153832	9.891797316909152
4	2.109088128407129	5.685262954066594
5	1.0684052362507597	3.5999946065403825
6	0.5861467025107795	2.370027659263527
7	0.3308024752468458	1.5604958875501853
8	0.20553608434359855	1.1080871114902988
9	0.1324191626262588	0.8031361688884795
>10	0.40166207691048406	3.9170781846519835
>50	0.0040292929466931564	0.17617618563241427
>100	8.596653488509509E-4	0.0878147401814647
>500	9.390071992042473E-5	0.041163477703819285
>1k	1.4085107721933734E-4	0.14440438848907436
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.261163477144838E-5	0.0
12-13	0.0	0.0	0.0	4.522326954289676E-5	0.0
14-15	0.0	0.0	0.0	4.522326954289676E-5	0.0
16-17	0.0	0.0	0.0	4.522326954289676E-5	0.0
18-19	0.0	0.0	0.0	4.522326954289676E-5	0.0
20-21	0.0	0.0	0.0	5.652908692862095E-5	0.0
22-23	0.0	0.0	0.0	1.0175235647151771E-4	0.0
24-25	0.0	0.0	0.0	3.052570694145531E-4	0.0
26-27	0.0	0.0	0.0	7.122664953006239E-4	0.0
28-29	0.0	0.0	0.0	0.003109099781074152	0.0
30-31	0.0	0.0	0.0	0.012018083881024814	0.0
32-33	0.0	0.0	0.0	0.028829834333596684	0.0
34-35	0.0	0.0	0.0	0.0576257512150362	2.261163477144838E-5
36-37	0.0	0.0	0.0	0.1065121055909076	2.261163477144838E-5
38-39	0.0	0.0	0.0	0.18262286823160284	2.261163477144838E-5
40-41	0.0	0.0	0.0	0.2653701456777182	2.261163477144838E-5
42-43	0.0	0.0	0.0	0.35357813292113827	2.261163477144838E-5
44-45	0.0	0.0	0.0	0.45833783681725865	2.261163477144838E-5
46-47	0.0	0.0	0.0	0.588920027622373	2.261163477144838E-5
48-49	0.0	0.0	0.0	0.7453812344234101	2.261163477144838E-5
50-51	0.0	0.0	0.0	0.9146406265050869	2.261163477144838E-5
52-53	0.0	0.0	0.0	1.0812431515011185	2.261163477144838E-5
54-55	0.0	0.0	0.0	1.260598638508247	2.261163477144838E-5
56-57	0.0	0.0	0.0	1.4826222803290987	2.261163477144838E-5
58-59	0.0	0.0	0.0	1.7532609369085645	2.261163477144838E-5
60-61	0.0	0.0	0.0	2.0333286451877237	2.261163477144838E-5
62-63	0.0	0.0	0.0	2.3294732257893838	2.261163477144838E-5
64-65	0.0	0.0	0.0	2.636403556177024	2.261163477144838E-5
66-67	0.0	0.0	0.0	2.982056311110769	2.261163477144838E-5
68-69	0.0	0.0	0.0	3.3710668757187676	2.261163477144838E-5
70-71	0.0	0.0	0.0	3.8115189094318103	2.261163477144838E-5
72-73	0.0	0.0	0.0	4.271914405013271	2.261163477144838E-5
74-75	0.0	0.0	0.0	4.71417536950803	2.261163477144838E-5
76-77	0.0	0.0	0.0	5.218765305250286	2.261163477144838E-5
78-79	0.0	0.0	0.0	5.7634230578075485	2.261163477144838E-5
80-81	0.0	2.261163477144838E-5	0.0	6.352546590142865	2.261163477144838E-5
82-83	0.0	2.261163477144838E-5	0.0	6.968544050404047	2.261163477144838E-5
84-85	0.0	2.261163477144838E-5	0.0	7.569120375751101	2.261163477144838E-5
86-87	0.0	2.261163477144838E-5	0.0	8.206338855245288	2.261163477144838E-5
88	0.0	2.261163477144838E-5	0.0	8.71445620601189	2.261163477144838E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5705	0.0	41.794147	94
GTACTAG	6345	0.0	36.609703	1
TAGGCAT	7640	0.0	33.462307	5
CAACGCA	7755	0.0	32.90549	5
GCATCGT	6590	0.0	32.589024	8
TACCCCG	355	0.0	31.771173	5
GTATCAA	9830	0.0	30.51884	1
TAGTACT	8395	0.0	30.286327	4
CTGTGCG	8415	0.0	29.765196	9
CTAACGC	3325	0.0	29.682308	3
ATCAACG	8640	0.0	29.64509	3
CTAGGCA	8850	0.0	29.525791	4
TCTAACG	3450	0.0	29.152084	2
TAACGCC	3420	0.0	29.132635	4
ACTGTGC	9100	0.0	28.402847	8
CGCCTAC	3500	0.0	28.330545	7
GTACTGT	9000	0.0	27.987736	6
TACTGTG	9920	0.0	26.860365	7
CTAGTAC	9965	0.0	26.835209	3
AACGCAG	10525	0.0	26.656166	6
>>END_MODULE