FastQCFastQC Report
Mon 16 Jan 2017
ERR1146657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4422502
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54770.12384392364322278No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47190.10670430448646491No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC58850.045.70837494
GTACTAG60300.035.8830951
GTATCAA133250.034.8063961
TAGTACT86650.033.7091564
CTGTGCG85700.033.404199
TAGGCAT76300.033.200925
GGTATCA83300.032.9774671
CAACGCA133050.032.4275175
ACTGTGC93700.031.7504588
TACTGTG100550.030.8020367
ATCAACG140600.030.6098063
GTACTGT94750.030.4551186
TATTCGC5150.030.1211249
CTAGGCA88150.029.613894
CTAGTAC103850.029.2131273
AACGCAG162800.027.7132856
TATCAAC168100.027.5904392
GCATCGT74900.027.4838898
CTAACGC29400.026.7173863
ACGCAGA163450.026.3669877
Produced by FastQC (version 0.11.5)