FastQCFastQC Report
Mon 16 Jan 2017
ERR1146656_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146656_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5444156
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC94990.1744806724862403No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC57900.040.2744894
GTACTAG65450.035.7064821
TACCCCG4850.034.8820275
CAACGCA122050.032.651195
TATTCTC29550.032.6014825
GTATCAA135300.032.5643271
TAGGCAT78450.031.8683515
GCATCGT68050.031.8341888
ATCAACG131750.030.4272923
CTGTGCG81950.029.5308049
TAGTACT83500.029.4923734
GGTATCA88100.029.2485921
CTAGGCA88800.028.9493774
TATCAAC157550.027.980362
AGGGCTA74550.027.925255
AACGCAG155750.027.244876
ACTGTGC91950.027.1371238
TCTAACG32750.026.6913472
CTAACGC32600.026.6697543
ACGCAGA147850.026.6662317
Produced by FastQC (version 0.11.5)