##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146656_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5444156
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05663926603132	31.0	31.0	34.0	28.0	34.0
2	31.1992729451544	31.0	31.0	34.0	27.0	34.0
3	31.323870954469342	31.0	31.0	34.0	27.0	34.0
4	34.761367051201326	37.0	35.0	37.0	31.0	37.0
5	34.91986122366809	37.0	35.0	37.0	32.0	37.0
6	34.98350359541497	37.0	35.0	37.0	32.0	37.0
7	34.90072474043727	37.0	35.0	37.0	31.0	37.0
8	34.85718502555768	37.0	35.0	37.0	31.0	37.0
9	36.293885222980386	39.0	37.0	39.0	30.0	39.0
10-11	36.129370190714596	39.0	37.0	39.0	30.0	39.0
12-13	36.0212318493445	39.0	35.5	39.0	30.0	39.0
14-15	37.141669066793824	40.0	37.0	41.0	30.0	41.0
16-17	37.168492875663375	40.0	37.0	41.0	30.0	41.0
18-19	37.14796710454293	40.0	36.5	41.0	30.0	41.0
20-21	37.21671348139179	40.0	37.0	41.0	30.0	41.0
22-23	37.16884049612098	40.0	36.5	41.0	30.0	41.0
24-25	37.12571333371049	40.0	36.0	41.0	30.0	41.0
26-27	37.1651456166943	40.0	36.0	41.0	30.0	41.0
28-29	37.16485613196977	40.0	36.0	41.0	30.0	41.0
30-31	37.035210140929095	40.0	36.0	41.0	30.0	41.0
32-33	37.02288352868654	40.0	36.0	41.0	30.0	41.0
34-35	36.95885330986107	40.0	36.0	41.0	30.0	41.0
36-37	36.83755388346697	40.0	35.0	41.0	30.0	41.0
38-39	36.72763712134626	40.0	35.0	41.0	30.0	41.0
40-41	36.63821481236026	39.0	35.0	41.0	29.0	41.0
42-43	36.478406478433016	39.0	35.0	41.0	29.0	41.0
44-45	36.31782685874542	39.0	35.0	41.0	28.5	41.0
46-47	36.313044299244915	39.0	35.0	41.0	28.5	41.0
48-49	36.16968314280487	39.0	35.0	41.0	28.0	41.0
50-51	35.980525907046015	38.5	34.5	40.5	28.0	41.0
52-53	35.76087450469825	38.0	34.0	40.0	28.0	41.0
54-55	35.54844883210547	38.0	34.0	40.0	27.5	41.0
56-57	35.292709650494956	37.0	34.0	40.0	27.0	41.0
58-59	34.980298231718564	37.0	33.0	40.0	26.5	41.0
60-61	34.728663543072614	36.0	33.0	40.0	26.0	41.0
62-63	34.415958782224465	36.0	33.0	39.0	26.0	41.0
64-65	34.255233960966585	35.0	33.0	39.0	26.0	41.0
66-67	34.000360294598465	35.0	33.0	39.0	26.0	41.0
68-69	33.87628826212915	35.0	33.0	38.5	26.0	41.0
70-71	33.81241793953002	35.0	33.0	38.0	26.0	41.0
72-73	33.605778104080784	35.0	33.0	37.0	26.0	40.0
74-75	33.36256529019374	35.0	33.0	37.0	26.0	39.5
76-77	33.11245976419485	35.0	32.5	36.0	25.5	39.0
78-79	32.87603083012316	35.0	32.0	36.0	25.0	39.0
80-81	32.7086198485128	35.0	32.0	36.0	25.0	38.5
82-83	32.45651107352545	35.0	32.0	35.0	25.0	37.0
84-85	32.286266778541986	35.0	32.0	35.0	25.0	37.0
86-87	32.115531222837845	35.0	32.0	35.0	25.0	36.5
88-89	31.992038619025614	35.0	32.0	35.0	24.0	36.0
90-91	31.844705405208813	35.0	32.0	35.0	24.0	36.0
92-93	31.694525928353265	35.0	31.0	35.0	24.0	36.0
94-95	31.626034962995185	35.0	31.0	35.0	24.0	35.5
96-97	31.52233771405522	35.0	31.0	35.0	23.5	35.0
98-99	31.43165451173699	35.0	31.0	35.0	23.0	35.0
100	30.45046725332632	34.0	29.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3909105075267014
1101	2	0.4938872868122317
1101	3	0.542123646072934
1101	4	0.6200529871741551
1101	5	0.49343161344440745
1101	6	0.5202875301133076
1101	7	0.5441670826490608
1101	8	0.6168857562647574
1101	9	0.7680874989617124
1101	10-11	0.8018410965841412
1101	12-13	0.7177303110115787
1101	14-15	0.9146548527113367
1101	16-17	0.8221603151372108
1101	18-19	0.8307153202807029
1101	20-21	0.7955169367133905
1101	22-23	0.8142951345629754
1101	24-25	0.8730864003777015
1101	26-27	0.7834542787762828
1101	28-29	0.7656057305553645
1101	30-31	0.8221250350524016
1101	32-33	0.7826594291570572
1101	34-35	0.8256545160041568
1101	36-37	0.7928461443708201
1101	38-39	0.7741749942520855
1101	40-41	0.8020283333212035
1101	42-43	0.7730713228644248
1101	44-45	0.8312789910068403
1101	46-47	0.7837468664849823
1101	48-49	0.7349411149302156
1101	50-51	0.6842942488999384
1101	52-53	0.6729160062901727
1101	54-55	0.6318140293474599
1101	56-57	0.6064081170267457
1101	58-59	0.580665066253701
1101	60-61	0.6125984693597104
1101	62-63	0.6060075881623774
1101	64-65	0.651519090312938
1101	66-67	0.652961026070372
1101	68-69	0.625125549885901
1101	70-71	0.6163356418336221
1101	72-73	0.6325012223146089
1101	74-75	0.6007909955461344
1101	76-77	0.5979845285126757
1101	78-79	0.5922526224805935
1101	80-81	0.5628136579980492
1101	82-83	0.5942557054941346
1101	84-85	0.5734206892489624
1101	86-87	0.5877924237208489
1101	88-89	0.6480384306579587
1101	90-91	0.6306074588414425
1101	92-93	0.598104795282488
1101	94-95	0.6462662856858934
1101	96-97	0.648893939326932
1101	98-99	0.5998105715949684
1101	100	0.6647418226270077
1102	1	0.3952976511688604
1102	2	0.48234433945456345
1102	3	0.5078700303157433
1102	4	0.6501549772579978
1102	5	0.5329001061717733
1102	6	0.5283764465262948
1102	7	0.4993444656470629
1102	8	0.4596213085002532
1102	9	0.6331631784698644
1102	10-11	0.7273251289368687
1102	12-13	0.6666461695039203
1102	14-15	0.8572021189160495
1102	16-17	0.8162861909622592
1102	18-19	0.8575737764860207
1102	20-21	0.8104338556035557
1102	22-23	0.8121623954345409
1102	24-25	0.8602520292833233
1102	26-27	0.800137806723555
1102	28-29	0.8439722257225242
1102	30-31	0.850437413795099
1102	32-33	0.809863224907744
1102	34-35	0.863438876444711
1102	36-37	0.861831526830521
1102	38-39	0.8922862712277535
1102	40-41	0.7720962374094
1102	42-43	0.8035442278934042
1102	44-45	0.7787625336576411
1102	46-47	0.730058937450643
1102	48-49	0.7516125373959497
1102	50-51	0.7561039031305015
1102	52-53	0.6882377641331843
1102	54-55	0.6946167318043237
1102	56-57	0.7287520759366402
1102	58-59	0.708133550258637
1102	60-61	0.7772687242663849
1102	62-63	0.7520801039219336
1102	64-65	0.7321606932955049
1102	66-67	0.7892379331977111
1102	68-69	0.6664033043346507
1102	70-71	0.6466532253726527
1102	72-73	0.6878122578036638
1102	74-75	0.6028529397250963
1102	76-77	0.6163757268369778
1102	78-79	0.6358102010852278
1102	80-81	0.6562539947335466
1102	82-83	0.703901930116551
1102	84-85	0.6692544784329044
1102	86-87	0.6227288185427184
1102	88-89	0.6550409377324691
1102	90-91	0.6881292929433904
1102	92-93	0.646952095504659
1102	94-95	0.7346865546107573
1102	96-97	0.7288668375025793
1102	98-99	0.698265518394404
1102	100	0.7135227763370899
1103	1	0.2913816367888096
1103	2	0.37932002424927447
1103	3	0.37337949378344604
1103	4	0.4776552109763301
1103	5	0.3706598947204256
1103	6	0.41507209426603
1103	7	0.43548539355133187
1103	8	0.45806737905768813
1103	9	0.5451317667261577
1103	10-11	0.60299611301852
1103	12-13	0.5520539442504457
1103	14-15	0.7024320977539134
1103	16-17	0.6447223603613139
1103	18-19	0.6688191977314375
1103	20-21	0.6263044351400211
1103	22-23	0.6889333004769114
1103	24-25	0.6302849623208928
1103	26-27	0.6176487914851165
1103	28-29	0.5917128493680153
1103	30-31	0.6298095613691075
1103	32-33	0.5896602299135694
1103	34-35	0.6084342020780866
1103	36-37	0.6409618396731247
1103	38-39	0.6738992251724838
1103	40-41	0.6098415354215234
1103	42-43	0.5757467894463133
1103	44-45	0.5078495023733751
1103	46-47	0.513118522876816
1103	48-49	0.5238595479260226
1103	50-51	0.5537164916247619
1103	52-53	0.5373977804509735
1103	54-55	0.5000820862488027
1103	56-57	0.5286209811999782
1103	58-59	0.5490199376531422
1103	60-61	0.5606264068004094
1103	62-63	0.5185002189963441
1103	64-65	0.49527165426029285
1103	66-67	0.5319226344207024
1103	68-69	0.5231300153653038
1103	70-71	0.509254212938842
1103	72-73	0.4361709601994903
1103	74-75	0.43959694591225684
1103	76-77	0.3742130248830904
1103	78-79	0.48635931168074364
1103	80-81	0.4157003797483796
1103	82-83	0.48102451750595776
1103	84-85	0.5311221428643762
1103	86-87	0.41647800052855644
1103	88-89	0.49537218034909003
1103	90-91	0.5022494496621626
1103	92-93	0.4402717881863012
1103	94-95	0.3974734119915766
1103	96-97	0.45519295531361337
1103	98-99	0.4403964346552449
1103	100	0.594427478749914
1104	1	0.29275445231824193
1104	2	0.3757089474613977
1104	3	0.3196825861344834
1104	4	0.49786898145168834
1104	5	0.3734992111304152
1104	6	0.38317836280789663
1104	7	0.4030738855204774
1104	8	0.43913913166648655
1104	9	0.48970212538866775
1104	10-11	0.6671050817578958
1104	12-13	0.48053510962741086
1104	14-15	0.6374487649378722
1104	16-17	0.6463396313381367
1104	18-19	0.6206401425404593
1104	20-21	0.5246070344565013
1104	22-23	0.5248937277065053
1104	24-25	0.6501556570160147
1104	26-27	0.6031657791927856
1104	28-29	0.4960288550472569
1104	30-31	0.4987870963563594
1104	32-33	0.5607544560264515
1104	34-35	0.5591247188708337
1104	36-37	0.5214719328026334
1104	38-39	0.5321420647275303
1104	40-41	0.5841715296083692
1104	42-43	0.585077131826921
1104	44-45	0.5393869269322096
1104	46-47	0.5298630622128044
1104	48-49	0.46432355650052415
1104	50-51	0.42110540780956995
1104	52-53	0.4858618030801054
1104	54-55	0.5270837502099468
1104	56-57	0.4970001926609058
1104	58-59	0.47046376421429414
1104	60-61	0.5021979558122212
1104	62-63	0.44292823326608755
1104	64-65	0.39544822290370973
1104	66-67	0.37242717328625474
1104	68-69	0.34719622166466735
1104	70-71	0.39790883151562895
1104	72-73	0.33535873297160634
1104	74-75	0.33306400736769604
1104	76-77	0.30766317393051423
1104	78-79	0.3813370814486845
1104	80-81	0.3518362812632674
1104	82-83	0.4199136569056634
1104	84-85	0.39526457919787106
1104	86-87	0.43978741365784657
1104	88-89	0.37625829590433213
1104	90-91	0.4301392529480559
1104	92-93	0.36855916555474977
1104	94-95	0.44557591701513743
1104	96-97	0.46870336140270297
1104	98-99	0.46733210772379863
1104	100	0.4876448203707966
1105	1	0.1714419075374245
1105	2	0.26567583331501865
1105	3	0.24337453027956713
1105	4	0.3765497207802966
1105	5	0.2883218421191174
1105	6	0.29342644953077013
1105	7	0.3271643469152181
1105	8	0.38044833550739554
1105	9	0.4737641710494316
1105	10-11	0.4898642330645231
1105	12-13	0.4013611861021076
1105	14-15	0.5355760678105881
1105	16-17	0.5030944050670954
1105	18-19	0.4829662157619552
1105	20-21	0.42777635686972104
1105	22-23	0.44897823722025265
1105	24-25	0.45456995273917755
1105	26-27	0.505423243862019
1105	28-29	0.5407427061069967
1105	30-31	0.5352564955057133
1105	32-33	0.47799550385521883
1105	34-35	0.5125007391440022
1105	36-37	0.4941379069808889
1105	38-39	0.5364760815290381
1105	40-41	0.47709031430672866
1105	42-43	0.5058973919707057
1105	44-45	0.47834916731189736
1105	46-47	0.5120032602854891
1105	48-49	0.39260666211956874
1105	50-51	0.4286933413677403
1105	52-53	0.46160005690123995
1105	54-55	0.39066491863590613
1105	56-57	0.376510892836734
1105	58-59	0.48482896788642904
1105	60-61	0.41608951791309323
1105	62-63	0.3966207691489032
1105	64-65	0.45454159049226917
1105	66-67	0.3498041352135317
1105	68-69	0.3556951134071724
1105	70-71	0.3721811111683664
1105	72-73	0.3987532094978832
1105	74-75	0.3758452715758125
1105	76-77	0.2842295525113556
1105	78-79	0.36231776561087514
1105	80-81	0.2941022284103738
1105	82-83	0.36742772749738606
1105	84-85	0.252176683445839
1105	86-87	0.24438550913479418
1105	88-89	0.2469084012587892
1105	90-91	0.27248033304266883
1105	92-93	0.27697876172985403
1105	94-95	0.2855335460302264
1105	96-97	0.17137370793378537
1105	98-99	0.1878319668995836
1105	100	0.39092648299757826
1106	1	0.08977979791729851
1106	2	0.2506091341499541
1106	3	0.23901344780477274
1106	4	0.33880796732741914
1106	5	0.19674252437679485
1106	6	0.24676607389609728
1106	7	0.2481359857416976
1106	8	0.20434751713580823
1106	9	0.42031381819840874
1106	10-11	0.40288267337543004
1106	12-13	0.2663758024771852
1106	14-15	0.42144689680105074
1106	16-17	0.32338530997355974
1106	18-19	0.3671977219456579
1106	20-21	0.31106580156127706
1106	22-23	0.3166064075131132
1106	24-25	0.366602774855032
1106	26-27	0.3834106953080507
1106	28-29	0.3629133271140432
1106	30-31	0.38673679430127805
1106	32-33	0.32186817463223605
1106	34-35	0.3451038828264288
1106	36-37	0.3681512977111865
1106	38-39	0.32440671452383185
1106	40-41	0.4321343720792612
1106	42-43	0.33440965142171564
1106	44-45	0.33381529958859346
1106	46-47	0.3232077716193018
1106	48-49	0.2514786769221402
1106	50-51	0.3241588793157817
1106	52-53	0.263424234886223
1106	54-55	0.33473098016219893
1106	56-57	0.2678136517545937
1106	58-59	0.2461522595946235
1106	60-61	0.25178651525726536
1106	62-63	0.2924913357495953
1106	64-65	0.24153170472034446
1106	66-67	0.21799271948790988
1106	68-69	0.2643641112331423
1106	70-71	0.24622175882220887
1106	72-73	0.1509006991074031
1106	74-75	0.21569389480400503
1106	76-77	0.17243742717629118
1106	78-79	0.19621489554091198
1106	80-81	0.22425200239160858
1106	82-83	0.19578733932490877
1106	84-85	0.19706430316907841
1106	86-87	0.17884966653326018
1106	88-89	0.2761347023248213
1106	90-91	0.21801753501293675
1106	92-93	0.28133851644915353
1106	94-95	0.27920076561221663
1106	96-97	0.25781836755891874
1106	98-99	0.2098569015155256
1106	100	0.1724528264569436
1107	1	0.03517952749785991
1107	2	0.11786359420798931
1107	3	0.07360180323947318
1107	4	0.23965959133107617
1107	5	0.1577650842702525
1107	6	0.22728690837078602
1107	7	0.10623436544778286
1107	8	0.10063182632809742
1107	9	0.17907897998517797
1107	10-11	0.27753160551918654
1107	12-13	0.10825571533437994
1107	14-15	0.29328143191159484
1107	16-17	0.19539553945441668
1107	18-19	0.18274601942707136
1107	20-21	0.17388855098000278
1107	22-23	0.16861248584584843
1107	24-25	0.21199790248405037
1107	26-27	0.1738387863715971
1107	28-29	0.24304511824054487
1107	30-31	0.30736683590355085
1107	32-33	0.22255980725125113
1107	34-35	0.19872248497895129
1107	36-37	0.20708408949604973
1107	38-39	0.23437012118263567
1107	40-41	0.21260283822377346
1107	42-43	0.2783108040070914
1107	44-45	0.31037561943361425
1107	46-47	0.22817483635999025
1107	48-49	0.24164749967093968
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2209	6	0.1569062599081832
2209	7	0.10479345452800004
2209	8	0.13574004183792
2209	9	0.18610420194343646
2209	10-11	0.18821172754535098
2209	12-13	0.15443016066069504
2209	14-15	0.28400067538916574
2209	16-17	0.18986574750039864
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2209	22-23	0.17517917155782214
2209	24-25	0.27437713494689575
2209	26-27	0.12384123347431597
2209	28-29	0.14352778850293646
2209	30-31	0.2909241164415661
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2209	34-35	0.04800581837852036
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2209	58-59	0.10887287011038893
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2209	90-91	0.2466139148303803
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2210	3	0.10964238482106836
2210	4	0.09720412231811082
2210	5	0.01691027767606812
2210	6	0.00853710810434194
2210	7	-0.014115566881102382
2210	8	0.0020519710248834144
2210	9	-0.001244043126078509
2210	10-11	-0.012585762833857927
2210	12-13	-0.08236709992046087
2210	14-15	-0.016344612347992893
2210	16-17	-0.07081299411265007
2210	18-19	-0.06506229535130359
2210	20-21	-0.031155549509740865
2210	22-23	-0.02473163848768678
2210	24-25	-0.0071977370818743225
2210	26-27	0.05684140461352882
2210	28-29	0.09761051773590879
2210	30-31	0.0555376659313751
2210	32-33	-0.01280498785052231
2210	34-35	0.07594126442477744
2210	36-37	0.01416391644559667
2210	38-39	0.0382871575771091
2210	40-41	-0.033387726551453056
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2210	60-61	0.12397447348975277
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2210	66-67	0.03774611256729088
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2211	1	0.010054309014151386
2211	2	-0.08440603192875074
2211	3	-0.09736563822341537
2211	4	0.04006727176742686
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2211	7	-0.046953260798382246
2211	8	-0.00796588049696112
2211	9	-0.03976873294906369
2211	10-11	-0.03029941974632777
2211	12-13	-0.055263085073193
2211	14-15	-0.050337774046852246
2211	16-17	-0.062121656785336654
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2211	24-25	0.055550488657431174
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2211	30-31	0.0770910287566835
2211	32-33	-0.056688714647776806
2211	34-35	-0.061879849144830246
2211	36-37	-0.10100170976279088
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2211	40-41	-0.0645369145773742
2211	42-43	-0.05120020627582278
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2211	54-55	0.07778770586160277
2211	56-57	0.08829318388866625
2211	58-59	-0.006619246183944938
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2211	66-67	0.028564376673671177
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2211	70-71	0.08220707841308439
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2211	74-75	0.11065029232773327
2211	76-77	0.06608135021986072
2211	78-79	0.0028510630880376198
2211	80-81	0.06544583020335892
2211	82-83	0.05601992179362725
2211	84-85	0.11701070832656058
2211	86-87	0.14442439244061944
2211	88-89	0.07046089601947969
2211	90-91	0.08855077611554307
2211	92-93	0.07359528221946476
2211	94-95	0.09771126266903352
2211	96-97	0.14951306906868211
2211	98-99	0.1779325277503112
2211	100	0.16566140315342537
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2212	36-37	-0.42296048102224404
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2212	62-63	-0.34807157736228334
2212	64-65	-0.4076801517695259
2212	66-67	-0.3296344326754621
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2212	74-75	-0.2468838805460507
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2212	78-79	-0.24969732426200153
2212	80-81	-0.22999254766586574
2212	82-83	-0.22640678718542517
2212	84-85	-0.21127221928568218
2212	86-87	-0.1766756763699675
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2212	92-93	-0.26103280784846206
2212	94-95	-0.1578500445781863
2212	96-97	-0.11827213921358748
2212	98-99	-0.20400638208286637
2212	100	-0.2158757064671306
2213	1	-0.26918049840095293
2213	2	-0.26335998066921107
2213	3	-0.38927948846173877
2213	4	-0.20493050924278577
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2213	26-27	-0.4193485087209652
2213	28-29	-0.585532677831516
2213	30-31	-0.5239724943119413
2213	32-33	-0.43923910639576036
2213	34-35	-0.5267878743161418
2213	36-37	-0.5257353379839671
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2213	44-45	-0.6019264620203586
2213	46-47	-0.5212104531441
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2213	50-51	-0.510126766712979
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2213	64-65	-0.37304014961615906
2213	66-67	-0.3404755896519589
2213	68-69	-0.4253216426811761
2213	70-71	-0.43555781951010886
2213	72-73	-0.3632948091699362
2213	74-75	-0.33149982068975703
2213	76-77	-0.3737589563727539
2213	78-79	-0.40830789787765553
2213	80-81	-0.25627476714256403
2213	82-83	-0.3501062653057261
2213	84-85	-0.3324838724364625
2213	86-87	-0.3601230594741551
2213	88-89	-0.2729546757960222
2213	90-91	-0.38403705451994696
2213	92-93	-0.28496055918359886
2213	94-95	-0.3388637205845946
2213	96-97	-0.24199248650268146
2213	98-99	-0.33319673726731835
2213	100	-0.14034048823993572
2214	1	-0.6421946733835959
2214	2	-0.8049622786983797
2214	3	-0.5990891871557231
2214	4	-0.6332127018335072
2214	5	-0.6016922724963294
2214	6	-0.5728538277774717
2214	7	-0.5726428022199386
2214	8	-0.6529108957468708
2214	9	-1.1880348711914337
2214	10-11	-0.7886674716325786
2214	12-13	-0.7243837001562383
2214	14-15	-3.296939318710912
2214	16-17	-1.5757794902014126
2214	18-19	-0.9692919487316018
2214	20-21	-0.7951001896632377
2214	22-23	-0.7722877461091358
2214	24-25	-0.6850847930134094
2214	26-27	-0.7740300904621549
2214	28-29	-0.6743645884160756
2214	30-31	-0.7928526406196781
2214	32-33	-0.8547273725670408
2214	34-35	-0.8272195260288342
2214	36-37	-0.8318053435992283
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2214	40-41	-0.8005128844758858
2214	42-43	-0.8440927948215631
2214	44-45	-0.7757121502825228
2214	46-47	-0.7859597555613718
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2214	50-51	-0.6671256315021523
2214	52-53	-0.6589074180788117
2214	54-55	-0.8646476761460562
2214	56-57	-0.8155817577624447
2214	58-59	-0.7704514616114508
2214	60-61	-0.7582158962850514
2214	62-63	-0.8206082742675562
2214	64-65	-0.6641576073101021
2214	66-67	-0.6769544193643497
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2214	70-71	-0.6412749340699762
2214	72-73	-0.6325148019220563
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2214	78-79	-0.6644128695832805
2214	80-81	-0.6678228984302059
2214	82-83	-0.6107118092744699
2214	84-85	-0.656084301816783
2214	86-87	-0.6511971762741453
2214	88-89	-0.7491271498097838
2214	90-91	-0.6804401504812851
2214	92-93	-0.6471769689473419
2214	94-95	-0.6024691923890906
2214	96-97	-0.6698845051908577
2214	98-99	-0.5712502417672418
2214	100	-0.3959704945592968
2215	1	-0.8770114516642593
2215	2	-1.2756579930019178
2215	3	-1.0437405716005337
2215	4	-2.5710287298645724
2215	5	-1.2646979726053473
2215	6	-0.9292849168555932
2215	7	-0.8849112349841874
2215	8	-0.7963238525977445
2215	9	-1.1090319771750714
2215	10-11	-0.8698583644256672
2215	12-13	-1.0830313509377874
2215	14-15	-1.1370635062884418
2215	16-17	-1.0945163034865217
2215	18-19	-1.0383680836916511
2215	20-21	-1.2155994706044524
2215	22-23	-1.1006180482847654
2215	24-25	-1.235860933445359
2215	26-27	-1.1733981842141858
2215	28-29	-1.0699837673382788
2215	30-31	-3.8497212557740497
2215	32-33	-1.7078279703598724
2215	34-35	-1.4208832570258991
2215	36-37	-1.185072529811535
2215	38-39	-1.0100692698832532
2215	40-41	-1.0902232117004331
2215	42-43	-1.0683156007786465
2215	44-45	-1.0199461230422884
2215	46-47	-1.2628770057909762
2215	48-49	-1.3320896410660552
2215	50-51	-1.154582920962433
2215	52-53	-1.1344969465709553
2215	54-55	-1.1385427869883884
2215	56-57	-1.165473385646898
2215	58-59	-1.1261783750794905
2215	60-61	-1.0531671644446163
2215	62-63	-0.9837209072939217
2215	64-65	-1.066512503958478
2215	66-67	-0.9895938754594198
2215	68-69	-0.8737440083235768
2215	70-71	-1.1703779530558975
2215	72-73	-1.1178567893064297
2215	74-75	-1.167764085171406
2215	76-77	-1.0510847748156635
2215	78-79	-0.9581957347547672
2215	80-81	-0.9087592162185274
2215	82-83	-0.7812909337881031
2215	84-85	-0.8826349494608863
2215	86-87	-0.9051979999081183
2215	88-89	-0.8629754636245437
2215	90-91	-0.9111227214177937
2215	92-93	-0.8946709466642346
2215	94-95	-0.9243799829274018
2215	96-97	-1.2457665494888914
2215	98-99	-1.1345302884188762
2215	100	-0.6155635521815519
2216	1	-0.5363720042410023
2216	2	-0.8976810529076431
2216	3	-0.8281515460191464
2216	4	-0.7103477771677333
2216	5	-0.6548838005220574
2216	6	-0.6119182913728807
2216	7	-0.7411165420136854
2216	8	-0.6088800400206793
2216	9	-0.8529837098898199
2216	10-11	-1.2677287401705257
2216	12-13	-0.8598175495446512
2216	14-15	-1.0041596720345183
2216	16-17	-1.0299602584108172
2216	18-19	-1.3049817782725341
2216	20-21	-1.5090851241112588
2216	22-23	-1.2699162276395128
2216	24-25	-1.241629516064279
2216	26-27	-1.696636466653196
2216	28-29	-1.5426813980688365
2216	30-31	-1.7482324639772528
2216	32-33	-1.8617522386104568
2216	34-35	-1.4820865087445085
2216	36-37	-1.6441215833752025
2216	38-39	-1.4593068484463103
2216	40-41	-1.3970705129420722
2216	42-43	-1.3700620255137608
2216	44-45	-1.2197557789696845
2216	46-47	-1.311309560239657
2216	48-49	-1.4285505762528032
2216	50-51	-1.4547324309010037
2216	52-53	-1.462152553773457
2216	54-55	-1.5423990559327407
2216	56-57	-1.393081769069525
2216	58-59	-1.2572314470156272
2216	60-61	-1.3516680960934053
2216	62-63	-1.4620821633147472
2216	64-65	-1.3850022840665588
2216	66-67	-1.3515274600252525
2216	68-69	-1.4337380751735722
2216	70-71	-1.2711683753503777
2216	72-73	-1.2637306868495912
2216	74-75	-1.4148522076079928
2216	76-77	-1.4055245399980194
2216	78-79	-1.2068623091721378
2216	80-81	-1.4105030993473093
2216	82-83	-1.309134609062287
2216	84-85	-1.3227884231824483
2216	86-87	-1.3340344801690165
2216	88-89	-1.3422756132100595
2216	90-91	-1.5093524159482392
2216	92-93	-1.3896740557592189
2216	94-95	-1.4590018653243995
2216	96-97	-1.4633365596224905
2216	98-99	-1.4799461850457618
2216	100	-1.0661654140625885
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	3.0
2	11.0
3	36.0
4	47.0
5	47.0
6	48.0
7	198.0
8	587.0
9	991.0
10	1699.0
11	3669.0
12	6668.0
13	9878.0
14	13432.0
15	16960.0
16	20185.0
17	23531.0
18	26073.0
19	29413.0
20	33435.0
21	37767.0
22	42544.0
23	49439.0
24	57163.0
25	65488.0
26	72574.0
27	78928.0
28	88948.0
29	105548.0
30	122865.0
31	140917.0
32	172566.0
33	222675.0
34	321303.0
35	454788.0
36	713570.0
37	1185572.0
38	950405.0
39	373722.0
40	463.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1120398614875	21.59688909260055	12.456751601932059	23.834319443979883
2	15.539917953104093	19.84517561165939	40.93057022078301	23.68433621445351
3	18.143157936504146	27.43508360315624	29.06041375580626	25.361344704533355
4	11.955818878417045	16.831900700860754	40.7792134311315	30.433066989590696
5	12.845378307474084	39.009503747089894	35.195973223893354	12.949144721542671
6	29.15118712935214	39.2144584465693	18.35731532701508	13.277039097063472
7	26.418772889433086	32.10817349992818	24.353217957181585	17.11983565345715
8	26.16904092850984	35.010997270795905	20.01213061238285	18.807831188311408
9	26.383785527295117	14.92363142353113	21.851118747478935	36.84146430169482
10-11	25.648141285501225	25.98362216300855	27.643190811820922	20.7250457396693
12-13	25.664193335165887	24.170918408347763	27.611855790302293	22.55303246618406
14-15	23.20953192133426	25.25790532184185	24.987477381680772	26.545085375143113
16-17	21.89896246881157	27.61130322950858	26.426668525551072	24.063065776128784
18-19	21.320554169899157	28.5728206495489	28.088578206325582	22.018046974226362
20-21	23.20623228072412	27.578455711694836	26.793055940400873	22.42225606718017
22-23	23.82271987144946	27.24269801469834	26.43632735678515	22.498254757067052
24-25	23.12932867383348	27.326010387439553	27.48026983463674	22.064391104090227
26-27	22.977078441824865	27.62092396745162	26.327719648094273	23.074277942629244
28-29	21.950002222662267	28.190402066745264	26.92031590459966	22.93927980599281
30-31	22.901177299801585	27.715697082701773	26.872608944157317	22.51051667333933
32-33	23.55442779034405	27.947458436466626	25.76166172021791	22.73645205297141
34-35	23.376496224892545	27.602149615233497	27.008178435110825	22.013175724763137
36-37	22.94177279042766	27.22853927859693	26.782361612316368	23.04732631865905
38-39	23.159525473065173	27.259709912938245	27.377780455655852	22.202984158340726
40-41	22.63490750218046	26.34367438651355	28.079170374245656	22.942247737060335
42-43	22.680100346598277	26.688131454741843	27.214252075497487	23.417516123162397
44-45	22.75833845458529	27.919706118219622	26.995354588136518	22.32660083905857
46-47	22.73147153854817	27.4789324978289	26.588566550048053	23.20102941357488
48-49	23.60007271812659	26.87701827019965	26.821294652728227	22.70161435894553
50-51	23.075227211828693	26.78951447460075	26.68914893865237	23.446109374918187
52-53	22.251884501453997	27.616918094291442	26.586685688904648	23.54451171534991
54-55	22.46048419321799	27.49793085709824	26.492258511962447	23.549326437721323
56-57	23.720083489879155	26.755671588226576	26.269706700601997	23.25453822129227
58-59	22.468041530302813	27.19713445760715	27.387336542499778	22.947487469590264
60-61	22.570713663056388	27.952355713630904	26.369451734302363	23.107478889010345
62-63	23.04768075504273	27.06343787777235	25.991775705604464	23.897105661580454
64-65	21.99973215830803	27.4187041679327	26.5491004523916	24.032463221367667
66-67	22.9325732408931	26.791347938125863	26.46695348637692	23.809125334604115
68-69	22.17592017699258	26.955382997749382	26.766619322669786	24.102077502588244
70-71	22.26383643483521	26.55069123619746	27.01670183606486	24.168770492902468
72-73	22.930132583056835	26.72457722548819	27.474060755202085	22.87122943625289
74-75	23.118097741382947	27.07944031679571	26.89060069352337	22.911861248297974
76-77	22.684700155542195	26.995489515184882	26.66801872825748	23.651791601015443
78-79	22.41638685086807	26.907581976323158	26.824133244615282	23.85189792819349
80-81	22.001112320998757	27.24350598802533	27.528695539556054	23.226686151419862
82-83	22.93458773131905	26.03966361609029	28.668967151501345	22.35678150108932
84-85	23.24944776351226	26.82469966959959	26.98165616845562	22.944196398432528
86-87	21.75068880910355	27.517754911723042	27.587588769966093	23.143967509207314
88-89	23.03072353357146	26.061221117610682	27.25457449064684	23.65348085817102
90-91	22.560081096172464	26.04467766411646	27.72456106816063	23.670680171550444
92-93	22.928518011165668	26.40833330424746	27.466400766691322	23.196747917895543
94-95	21.511650602165076	26.45063409170713	28.201556230317383	23.836159075810407
96-97	21.925657435845434	26.36410830972309	27.692959691645857	24.01727456278562
98-99	21.150084520868994	27.2827615269675	27.521306570289727	24.045847381873774
100	22.208293623937053	26.40703857681524	26.518049934853572	24.86661786439414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	901.0
1	624.0
2	420.5
3	493.5
4	446.0
5	391.0
6	367.5
7	362.5
8	398.5
9	424.0
10	394.5
11	359.5
12	347.0
13	341.5
14	406.5
15	784.5
16	970.5
17	992.0
18	1401.5
19	1735.5
20	2088.5
21	2698.5
22	3738.5
23	5049.0
24	6254.5
25	8027.5
26	11231.5
27	14751.5
28	19988.0
29	31541.5
30	48213.5
31	63638.0
32	85839.0
33	106527.0
34	117543.0
35	133250.5
36	148003.5
37	175612.5
38	186050.5
39	167022.0
40	150250.0
41	133641.0
42	129772.0
43	139842.5
44	155520.0
45	191326.5
46	226383.5
47	249637.5
48	264209.5
49	256678.0
50	241404.5
51	245733.5
52	249430.5
53	238202.5
54	225485.5
55	194257.5
56	169492.5
57	138827.0
58	106609.5
59	102618.5
60	86019.5
61	58684.0
62	43395.5
63	36326.0
64	26310.0
65	12545.0
66	6721.0
67	4434.5
68	3185.0
69	2325.5
70	1639.5
71	1163.5
72	873.5
73	624.0
74	419.5
75	324.0
76	191.0
77	78.0
78	54.0
79	48.5
80	41.0
81	30.5
82	26.5
83	20.0
84	17.0
85	16.0
86	13.0
87	13.0
88	9.0
89	8.0
90	7.5
91	5.0
92	5.5
93	9.0
94	8.5
95	4.5
96	6.5
97	9.0
98	6.5
99	8.0
100	17.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05352528472732964
2	0.008082060837345586
3	0.008486163879212867
4	0.010359732527870253
5	0.0036369273768055137
6	0.002589933131967563
7	9.184160042438167E-4
8	0.001432728966620354
9	5.877862427160427E-4
10-11	5.602337625887281E-4
12-13	2.663406412307068E-4
14-15	1.2857824059413434E-4
16-17	5.602337625887281E-4
18-19	0.0015704913672569265
20-21	9.092318442013784E-4
22-23	0.0013133348860686577
24-25	0.001487833926874983
26-27	0.0036369273768055137
28-29	0.004298186899861062
30-31	0.005519680185505338
32-33	0.00645646450983403
34-35	0.003765505617399648
36-37	0.008679031240104067
38-39	0.00691567251195594
40-41	0.0064656486698764695
42-43	0.015107943269810785
44-45	0.02114193641769266
46-47	0.019415314329714283
48-49	0.02243690298367644
50-51	0.01933265688933234
52-53	0.025779937239123932
54-55	0.019856154011751316
56-57	0.01582430775312096
58-59	0.018331583444706583
60-61	0.016549856396473574
62-63	0.01193022389512718
64-65	0.011792461494490607
66-67	0.01438239462645817
68-69	0.005730915866481416
70-71	0.009239265002692796
72-73	0.008706583720231382
74-75	0.011902671414999864
76-77	0.011333253492368698
78-79	0.012747614138904175
80-81	0.010717914769525341
82-83	0.0069064883519135015
84-85	0.011195491091732125
86-87	0.009579078924263008
88-89	0.008495348039255305
90-91	0.01363847766302068
92-93	0.009276001642862549
94-95	0.007797351876030004
96-97	0.0100107344462576
98-99	0.009735209644984456
100	0.25864431511514363
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5444156.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.86520270539662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82583737008123	47.14902068535881
2	14.893269601560233	19.023233640995315
3	5.447306465993929	10.436799949473597
4	2.422914254460466	6.1895963999565025
5	1.2576394648931268	4.0159699677853045
6	0.7236353714817313	2.772907181068545
7	0.44332906905613273	1.9819310602325284
8	0.2740243437898077	1.4000496209879487
9	0.17625800026936164	1.013107762408557
>10	0.5266502712604696	4.907755669926061
>50	0.006624638797147667	0.2823367462330176
>100	0.0023346790949044056	0.2723162687676812
>500	0.0	0.0
>1k	1.3235194604490472E-4	0.28733490010304213
>5k	4.4117315348301574E-5	0.2676401467031572
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	9499	0.1744806724862403	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.8368320084876333E-5	0.0
5	0.0	0.0	0.0	1.8368320084876333E-5	1.8368320084876333E-5
6	0.0	0.0	0.0	1.8368320084876333E-5	1.8368320084876333E-5
7	0.0	0.0	0.0	1.8368320084876333E-5	1.8368320084876333E-5
8	0.0	0.0	0.0	1.8368320084876333E-5	1.8368320084876333E-5
9	0.0	0.0	0.0	1.2857824059413434E-4	1.8368320084876333E-5
10-11	0.0	0.0	0.0	1.2857824059413434E-4	1.8368320084876333E-5
12-13	0.0	0.0	0.0	1.3776240063657251E-4	3.6736640169752666E-5
14-15	0.0	0.0	0.0	1.4694656067901066E-4	3.6736640169752666E-5
16-17	0.0	0.0	0.0	2.0205152093363967E-4	3.6736640169752666E-5
18-19	0.0	0.0	0.0	2.20419841018516E-4	3.6736640169752666E-5
20-21	0.0	0.0	0.0	2.20419841018516E-4	3.6736640169752666E-5
22-23	0.0	0.0	0.0	2.663406412307068E-4	3.6736640169752666E-5
24-25	0.0	0.0	0.0	3.6736640169752663E-4	3.6736640169752666E-5
26-27	0.0	0.0	0.0	7.255486433526152E-4	7.347328033950533E-5
28-29	0.0	0.0	0.0	0.0032328243349382347	7.347328033950533E-5
30-31	0.0	0.0	0.0	0.011774093174405729	7.347328033950533E-5
32-33	0.0	0.0	0.0	0.027120824605319907	8.265744038194351E-5
34-35	0.0	0.0	0.0	0.05035674951268847	9.184160042438167E-5
36-37	0.0	0.0	0.0	0.08839754040846735	9.184160042438167E-5
38-39	0.0	0.0	0.0	0.14702003395935015	9.184160042438167E-5
40-41	0.0	0.0	0.0	0.21175917809849681	9.184160042438167E-5
42-43	0.0	0.0	0.0	0.2809250873780986	9.184160042438167E-5
44-45	0.0	0.0	0.0	0.3610293312682443	1.1020992050925801E-4
46-47	0.0	0.0	0.0	0.4604019429274253	1.2857824059413434E-4
48-49	0.0	0.0	0.0	0.5784735044330104	1.2857824059413434E-4
50-51	0.0	0.0	0.0	0.706721115265617	1.4694656067901066E-4
52-53	0.0	0.0	0.0	0.831396087841715	1.4694656067901066E-4
54-55	0.0	0.0	0.0	0.9673767614300546	1.4694656067901066E-4
56-57	0.0	0.0	0.0	1.1326732738738565	1.4694656067901066E-4
58-59	0.0	0.0	0.0	1.3351656344895333	1.4694656067901066E-4
60-61	0.0	0.0	0.0	1.5490739060379606	1.4694656067901066E-4
62-63	0.0	0.0	0.0	1.7734704883548524	2.0205152093363967E-4
64-65	0.0	0.0	0.0	2.0081257774391474	2.20419841018516E-4
66-67	0.0	0.0	0.0	2.279811599814553	2.2960400106095417E-4
68-69	0.0	0.0	0.0	2.582236805852	2.3878816110339232E-4
70-71	0.0	0.0	0.0	2.916448389796325	2.3878816110339232E-4
72-73	0.0	0.0	0.0	3.272784615282883	2.571564811882686E-4
74-75	0.0	0.0	0.0	3.618632530000977	2.75524801273145E-4
76-77	0.0	0.0	0.0	4.018281254247674	2.75524801273145E-4
78-79	0.0	0.0	0.0	4.451984843931731	2.75524801273145E-4
80-81	0.0	0.0	0.0	4.924142511713478	2.75524801273145E-4
82-83	0.0	0.0	0.0	5.425762964911366	2.75524801273145E-4
84-85	0.0	0.0	0.0	5.9115131895559205	2.75524801273145E-4
86-87	0.0	0.0	0.0	6.432613246203819	2.75524801273145E-4
88	0.0	0.0	0.0	6.848958773407669	2.75524801273145E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5790	0.0	40.27448	94
GTACTAG	6545	0.0	35.706482	1
TACCCCG	485	0.0	34.882027	5
CAACGCA	12205	0.0	32.65119	5
TATTCTC	2955	0.0	32.601482	5
GTATCAA	13530	0.0	32.564327	1
TAGGCAT	7845	0.0	31.868351	5
GCATCGT	6805	0.0	31.834188	8
ATCAACG	13175	0.0	30.427292	3
CTGTGCG	8195	0.0	29.530804	9
TAGTACT	8350	0.0	29.492373	4
GGTATCA	8810	0.0	29.248592	1
CTAGGCA	8880	0.0	28.949377	4
TATCAAC	15755	0.0	27.98036	2
AGGGCTA	7455	0.0	27.92525	5
AACGCAG	15575	0.0	27.24487	6
ACTGTGC	9195	0.0	27.137123	8
TCTAACG	3275	0.0	26.691347	2
CTAACGC	3260	0.0	26.669754	3
ACGCAGA	14785	0.0	26.666231	7
>>END_MODULE