FastQCFastQC Report
Mon 16 Jan 2017
ERR1146656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5444156
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165680.3043263271662311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133470.2451619681728444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93410.17157847791282982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124600.044.3607751
GTATCAA220800.040.2450261
GGGCACC57600.039.8401594
GTGGTAT29150.034.6959841
TACCCCG4200.033.5706865
CAACGCA265200.032.271915
GTACTAG60400.032.2435381
ATCAACG269000.031.7030373
CTGTGCG80600.030.7346069
TATCAAC288600.030.7251722
CAGTACG8650.029.8881239
TAGGCAT76350.029.547485
CTAACGC29250.029.107713
ACTGTGC88900.029.023578
AACGCAG305550.028.9935446
TAGTACT85150.028.9468964
CGCCTAC29500.028.8355887
TATTCTC33900.028.559845
TCTAACG30500.028.0705452
TACTGTG102900.027.677677
Produced by FastQC (version 0.11.5)