FastQCFastQC Report
Mon 16 Jan 2017
ERR1146655_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146655_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5731948
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC96870.16900013747507828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC65050.043.53539794
GTACTAG74900.034.0265731
CAACGCA104250.032.682015
GTATCAA124350.032.1798861
GCATCGT74000.032.1323788
TAGGCAT85500.031.8243275
TATTCTC32400.031.619745
CTGTGCG95850.030.9357999
ACTGTGC99850.030.5436368
ATCAACG111700.030.5043663
TAGTACT99850.030.311764
CTAGGCA96800.029.0820374
AGGGCTA75700.029.0533895
GGTATCA81050.028.833951
GTACTGT106700.028.5835236
TACTGTG115050.027.9789777
CTAGTAC117700.027.2323463
CTATTGA80000.027.196639
GCTATTG80750.027.1186148
TATCAAC146450.026.9892832
Produced by FastQC (version 0.11.5)