##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146655_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5731948
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.152657700314098	31.0	31.0	34.0	28.0	34.0
2	31.26900453388621	31.0	31.0	34.0	28.0	34.0
3	31.415139146412354	33.0	31.0	34.0	28.0	34.0
4	34.84299979692768	37.0	35.0	37.0	32.0	37.0
5	35.031633399326026	37.0	35.0	37.0	32.0	37.0
6	35.08357071627307	37.0	35.0	37.0	32.0	37.0
7	34.97555543071919	37.0	35.0	37.0	32.0	37.0
8	35.000749308960934	37.0	35.0	37.0	32.0	37.0
9	36.45802369456248	39.0	37.0	39.0	32.0	39.0
10-11	36.290178400083185	39.0	37.0	39.0	31.0	39.0
12-13	36.20739223384441	39.0	37.0	39.0	30.5	39.0
14-15	37.35516686473778	40.0	37.0	41.0	31.0	41.0
16-17	37.39336269275297	40.0	37.0	41.0	31.0	41.0
18-19	37.35774077154922	40.0	37.0	41.0	31.0	41.0
20-21	37.417583079958156	40.0	37.0	41.0	31.0	41.0
22-23	37.37355058001224	40.0	37.0	41.0	30.5	41.0
24-25	37.326524595128916	40.0	37.0	41.0	30.0	41.0
26-27	37.3626625712585	40.0	36.5	41.0	30.0	41.0
28-29	37.35973014758682	40.0	36.0	41.0	30.0	41.0
30-31	37.22902589137236	40.0	36.0	41.0	30.0	41.0
32-33	37.220387205187485	40.0	36.0	41.0	30.0	41.0
34-35	37.1670971195133	40.0	36.0	41.0	30.0	41.0
36-37	37.05231615848574	40.0	36.0	41.0	30.0	41.0
38-39	36.9410242381822	40.0	35.0	41.0	30.0	41.0
40-41	36.846847180051185	39.5	35.0	41.0	30.0	41.0
42-43	36.69036215960089	39.0	35.0	41.0	29.5	41.0
44-45	36.51590480234643	39.0	35.0	41.0	29.0	41.0
46-47	36.50754324707761	39.0	35.0	41.0	29.0	41.0
48-49	36.35988812180432	39.0	35.0	41.0	29.0	41.0
50-51	36.16942800248711	39.0	35.0	41.0	28.0	41.0
52-53	35.94337623090789	38.0	34.5	40.0	28.0	41.0
54-55	35.72077782282742	38.0	34.0	40.0	28.0	41.0
56-57	35.463117425349985	37.5	34.0	40.0	28.0	41.0
58-59	35.1404332349142	37.0	34.0	40.0	27.0	41.0
60-61	34.88941979236378	36.5	33.0	40.0	27.0	41.0
62-63	34.580349908966376	36.0	33.0	39.0	26.5	41.0
64-65	34.41944248273013	35.0	33.0	39.0	26.0	41.0
66-67	34.16421197470738	35.0	33.0	39.0	26.0	41.0
68-69	34.04156832895204	35.0	33.0	38.5	26.0	41.0
70-71	33.98118161574389	35.0	33.0	38.0	26.0	41.0
72-73	33.776280943232564	35.0	33.0	37.0	26.0	40.0
74-75	33.533860739839234	35.0	33.0	37.0	26.0	39.5
76-77	33.28459216657234	35.0	33.0	36.0	26.0	39.0
78-79	33.04553800906777	35.0	33.0	36.0	26.0	39.0
80-81	32.876136960767965	35.0	33.0	36.0	25.5	38.5
82-83	32.61935994534493	35.0	32.0	35.0	25.0	37.0
84-85	32.45337056442243	35.0	32.0	35.0	25.0	37.0
86-87	32.28386056537847	35.0	32.0	35.0	25.0	36.5
88-89	32.16151489859992	35.0	32.0	35.0	25.0	36.0
90-91	32.01299750102409	35.0	32.0	35.0	25.0	36.0
92-93	31.85874252522877	35.0	32.0	35.0	24.0	36.0
94-95	31.79242868218623	35.0	32.0	35.0	24.0	35.5
96-97	31.684987023608727	35.0	32.0	35.0	24.0	35.0
98-99	31.59498350299061	35.0	31.5	35.0	24.0	35.0
100	30.6213173950636	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4244927286757907
1101	2	0.5347088825465072
1101	3	0.570691674420047
1101	4	0.6063042556342708
1101	5	0.5321169440857929
1101	6	0.5027795650635767
1101	7	0.5099545488095671
1101	8	0.5694444598476736
1101	9	0.756114787704
1101	10-11	0.7625708571267467
1101	12-13	0.6649691562601348
1101	14-15	0.9078902415629813
1101	16-17	0.8717540206846408
1101	18-19	0.8648654137005138
1101	20-21	0.8159321120863083
1101	22-23	0.8327717809684074
1101	24-25	0.8976403684953738
1101	26-27	0.8240802404852232
1101	28-29	0.8348377172714265
1101	30-31	0.8885376202782425
1101	32-33	0.8587265097010288
1101	34-35	0.8218439536770745
1101	36-37	0.9063373526961342
1101	38-39	0.8458463073627129
1101	40-41	0.8617034844620122
1101	42-43	0.8125985003531682
1101	44-45	0.8066383195584876
1101	46-47	0.8077426884538923
1101	48-49	0.7873984983042206
1101	50-51	0.7224564878751494
1101	52-53	0.7367901507006849
1101	54-55	0.6862856811264137
1101	56-57	0.660260701745905
1101	58-59	0.7090172386308495
1101	60-61	0.7055381286095397
1101	62-63	0.6951810181554094
1101	64-65	0.6876137401494233
1101	66-67	0.6879730333442851
1101	68-69	0.7368844269057675
1101	70-71	0.6892532124651396
1101	72-73	0.66956844852659
1101	74-75	0.6286154491935108
1101	76-77	0.6816101053832639
1101	78-79	0.6315678930488744
1101	80-81	0.6393892979852112
1101	82-83	0.6398813940201649
1101	84-85	0.6044568749807198
1101	86-87	0.6059770761548791
1101	88-89	0.6541299421749756
1101	90-91	0.5909869231227987
1101	92-93	0.5814115494372878
1101	94-95	0.6337873845036697
1101	96-97	0.6267491934375364
1101	98-99	0.605573639160756
1101	100	0.7044959994596631
1102	1	0.37102959923796774
1102	2	0.44953354787775623
1102	3	0.44186435861000106
1102	4	0.5686528760417318
1102	5	0.4440529794010857
1102	6	0.46260587931020325
1102	7	0.4935178905706863
1102	8	0.5051601451043339
1102	9	0.6384398652640968
1102	10-11	0.7520970000693836
1102	12-13	0.6607028040070304
1102	14-15	0.774003198373606
1102	16-17	0.7402244939029927
1102	18-19	0.7955778876435744
1102	20-21	0.751771885859192
1102	22-23	0.8072534795344666
1102	24-25	0.771590737160281
1102	26-27	0.771983675114889
1102	28-29	0.7799815087996649
1102	30-31	0.799160261413185
1102	32-33	0.7741633475902461
1102	34-35	0.824321779836616
1102	36-37	0.8799969855172876
1102	38-39	0.8666981273785126
1102	40-41	0.7748455308881432
1102	42-43	0.8364544889695438
1102	44-45	0.8006256437759589
1102	46-47	0.8176416135035538
1102	48-49	0.7653014290986278
1102	50-51	0.7519367665418528
1102	52-53	0.7180175926222248
1102	54-55	0.658271792067886
1102	56-57	0.6800133339034247
1102	58-59	0.6600001626870977
1102	60-61	0.620099807205392
1102	62-63	0.6544317006231637
1102	64-65	0.7307433102914302
1102	66-67	0.7248371830739373
1102	68-69	0.6547951722358718
1102	70-71	0.6127884514153692
1102	72-73	0.623383715549096
1102	74-75	0.6187906108279293
1102	76-77	0.6218262863323858
1102	78-79	0.6507597158535887
1102	80-81	0.628951765414044
1102	82-83	0.6318049148820037
1102	84-85	0.6434743097162396
1102	86-87	0.6294366380319545
1102	88-89	0.6963692506890808
1102	90-91	0.6178542307289803
1102	92-93	0.6364211350302504
1102	94-95	0.6831311009146575
1102	96-97	0.6200718749810896
1102	98-99	0.5777231275898451
1102	100	0.7138656811986372
1103	1	0.2631409060675516
1103	2	0.3724192309476102
1103	3	0.41211118685589554
1103	4	0.5090313311540768
1103	5	0.3551884933850431
1103	6	0.42675558164470573
1103	7	0.36724863150136855
1103	8	0.4268458757412148
1103	9	0.5402377500181217
1103	10-11	0.629657629440544
1103	12-13	0.5705935856669697
1103	14-15	0.717006572659173
1103	16-17	0.716886552514481
1103	18-19	0.7366945606277397
1103	20-21	0.6325707888447667
1103	22-23	0.7004079162740382
1103	24-25	0.6859229036151291
1103	26-27	0.6255199858464593
1103	28-29	0.6100806563094565
1103	30-31	0.614565844592299
1103	32-33	0.6184828047316202
1103	34-35	0.6630643753608538
1103	36-37	0.7114421310797994
1103	38-39	0.7432955245705699
1103	40-41	0.6896600566672362
1103	42-43	0.6773859107032152
1103	44-45	0.6274068057491036
1103	46-47	0.63230191808114
1103	48-49	0.6481289912144774
1103	50-51	0.5740903634262082
1103	52-53	0.5521839062524023
1103	54-55	0.5977548351680682
1103	56-57	0.6005461217024859
1103	58-59	0.6336355837667185
1103	60-61	0.6133255944767626
1103	62-63	0.5921135144131426
1103	64-65	0.5872601780030848
1103	66-67	0.6401396424375818
1103	68-69	0.5698105571942946
1103	70-71	0.520146103388285
1103	72-73	0.5497518248343312
1103	74-75	0.5143024022465426
1103	76-77	0.5492837918880582
1103	78-79	0.5125355265368441
1103	80-81	0.44873407196863013
1103	82-83	0.48611270323849
1103	84-85	0.5409991523362905
1103	86-87	0.5640438756515209
1103	88-89	0.6278028761136483
1103	90-91	0.5902579260919367
1103	92-93	0.5379544282289039
1103	94-95	0.5072613304998512
1103	96-97	0.5814331465070275
1103	98-99	0.5952599362886595
1103	100	0.7018977443438565
1104	1	0.228430942557452
1104	2	0.3878960925195969
1104	3	0.3473189211298795
1104	4	0.5334202511176827
1104	5	0.4223349301684962
1104	6	0.37641392182703726
1104	7	0.4105987476122266
1104	8	0.4748166765281354
1104	9	0.5654725423883775
1104	10-11	0.7084049424094445
1104	12-13	0.5531439394690167
1104	14-15	0.7042881552867684
1104	16-17	0.664393456814004
1104	18-19	0.6399560870583088
1104	20-21	0.6518513293552175
1104	22-23	0.6196026954300464
1104	24-25	0.6553062945480974
1104	26-27	0.658715865217566
1104	28-29	0.6096271032053622
1104	30-31	0.5312740853395823
1104	32-33	0.620781770563454
1104	34-35	0.6680767473113391
1104	36-37	0.6311853769477338
1104	38-39	0.6043403618610057
1104	40-41	0.6390625834670516
1104	42-43	0.6994033396079473
1104	44-45	0.693028748161133
1104	46-47	0.6336274390607031
1104	48-49	0.6140225416348812
1104	50-51	0.5117147423066513
1104	52-53	0.5385232200302994
1104	54-55	0.6063355102762031
1104	56-57	0.6069104565013888
1104	58-59	0.5401877646162205
1104	60-61	0.5313287939975879
1104	62-63	0.5584582296006033
1104	64-65	0.5972426185602302
1104	66-67	0.5595630699212322
1104	68-69	0.5072073147927867
1104	70-71	0.504940175256209
1104	72-73	0.482962180080321
1104	74-75	0.44294282345925495
1104	76-77	0.44430165397515253
1104	78-79	0.4583457634963324
1104	80-81	0.4497388143673362
1104	82-83	0.505658233693616
1104	84-85	0.5147434947730147
1104	86-87	0.4826967514645659
1104	88-89	0.49854019282853557
1104	90-91	0.5214883073920689
1104	92-93	0.5012376538865233
1104	94-95	0.600899104487084
1104	96-97	0.5675726057000041
1104	98-99	0.5453981863632329
1104	100	0.6145681701193269
1105	1	0.1969593297528398
1105	2	0.30670945214054157
1105	3	0.25458640308616154
1105	4	0.39187637400626585
1105	5	0.20717163805845473
1105	6	0.26506467276863077
1105	7	0.28483383371014526
1105	8	0.3543110583356608
1105	9	0.444975664361003
1105	10-11	0.4430585396557447
1105	12-13	0.31017760825889695
1105	14-15	0.47974323452072554
1105	16-17	0.38921240148413716
1105	18-19	0.3936622757551902
1105	20-21	0.3954511611231766
1105	22-23	0.43819117693982435
1105	24-25	0.42069759975055376
1105	26-27	0.3725183877511711
1105	28-29	0.4450929989615702
1105	30-31	0.4856930482504893
1105	32-33	0.4760394174785745
1105	34-35	0.4426868881219761
1105	36-37	0.44808196097078223
1105	38-39	0.5002524084915478
1105	40-41	0.4991075272851262
1105	42-43	0.4853214187458761
1105	44-45	0.4494842508447334
1105	46-47	0.4896527379049971
1105	48-49	0.3284497229313956
1105	50-51	0.3646066082666195
1105	52-53	0.44063604315097393
1105	54-55	0.45342932885839815
1105	56-57	0.4035356085396131
1105	58-59	0.4526602779328357
1105	60-61	0.48059541164599295
1105	62-63	0.38286629803320693
1105	64-65	0.399308541309324
1105	66-67	0.35314235811644323
1105	68-69	0.3415643215308748
1105	70-71	0.3478932419804721
1105	72-73	0.30653073233976613
1105	74-75	0.3188177845290525
1105	76-77	0.3236073091943581
1105	78-79	0.346513587435787
1105	80-81	0.26916288435637625
1105	82-83	0.32967918813781694
1105	84-85	0.2761846219774
1105	86-87	0.23612122228163912
1105	88-89	0.2709743912221967
1105	90-91	0.29611278398650853
1105	92-93	0.3287601862952023
1105	94-95	0.34705666008361646
1105	96-97	0.2910689816521206
1105	98-99	0.22501847915660989
1105	100	0.29572651675460904
1106	1	0.05357271432858823
1106	2	0.1893176010339026
1106	3	0.1831474799129751
1106	4	0.30146423654736765
1106	5	0.11466499760598481
1106	6	0.22110042999491242
1106	7	0.18010139947056558
1106	8	0.18462679421435269
1106	9	0.29383904280238227
1106	10-11	0.33581944962777754
1106	12-13	0.21164740263491666
1106	14-15	0.34871123459434017
1106	16-17	0.2782710291648627
1106	18-19	0.31141728139881053
1106	20-21	0.2992957223932322
1106	22-23	0.34162569410378296
1106	24-25	0.31306993590160914
1106	26-27	0.3521053224692352
1106	28-29	0.3233911798149762
1106	30-31	0.4447833369338099
1106	32-33	0.36568694440844496
1106	34-35	0.3652646565618838
1106	36-37	0.40167911554456737
1106	38-39	0.3778779176440281
1106	40-41	0.444323794400006
1106	42-43	0.35435670515948203
1106	44-45	0.3960171065153446
1106	46-47	0.397000981328965
1106	48-49	0.3045306751078698
1106	50-51	0.3815593153628569
1106	52-53	0.36325349327857026
1106	54-55	0.39757725758932594
1106	56-57	0.3784638747834137
1106	58-59	0.3632566687779075
1106	60-61	0.3751973985675221
1106	62-63	0.35449120755129115
1106	64-65	0.3299784985527836
1106	66-67	0.3442854821042758
1106	68-69	0.3846592094559824
1106	70-71	0.3712164893917631
1106	72-73	0.3157756070759916
1106	74-75	0.3563428587548927
1106	76-77	0.35658432525413275
1106	78-79	0.32662539394558365
1106	80-81	0.32385172624920244
1106	82-83	0.31542886148275784
1106	84-85	0.2515689400811496
1106	86-87	0.2581643039423369
1106	88-89	0.32353901678042263
1106	90-91	0.32981039649079946
1106	92-93	0.3933268929374911
1106	94-95	0.41383844837434225
1106	96-97	0.34554522938240595
1106	98-99	0.36533446040998285
1106	100	0.3987608814795429
1107	1	0.12039879264511555
1107	2	0.17545764023997634
1107	3	0.1419429987061953
1107	4	0.20674958626437956
1107	5	0.13052579063256076
1107	6	0.17319942683332812
1107	7	0.14046669282883784
1107	8	0.1358879553955532
1107	9	0.1438289200443279
1107	10-11	0.3242539184033575
1107	12-13	0.2425850352393084
1107	14-15	0.3698327801564574
1107	16-17	0.21626922948042449
1107	18-19	0.18728415928622155
1107	20-21	0.26024223388910883
1107	22-23	0.35747789585968803
1107	24-25	0.343657380842771
1107	26-27	0.2820451910452064
1107	28-29	0.2976311885407199
1107	30-31	0.37634088574716174
1107	32-33	0.3235208945701089
1107	34-35	0.297279036854853
1107	36-37	0.3511523585923655
1107	38-39	0.3895139533179659
1107	40-41	0.3272770698406333
1107	42-43	0.4014127755272128
1107	44-45	0.35183677723807705
1107	46-47	0.34023331991766526
1107	48-49	0.3397135160244318
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2209	4	0.14014209130323252
2209	5	0.1756839745858656
2209	6	0.0643776290747553
2209	7	0.10565070007769606
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2210	4	0.11781616484734769
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2214	1	-0.5781993608676395
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2214	3	-0.5812293234685733
2214	4	-0.48764702867153176
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2214	90-91	-0.6106498959555893
2214	92-93	-0.5872443168200832
2214	94-95	-0.5552818954105305
2214	96-97	-0.6044000453064164
2214	98-99	-0.4817983364903675
2214	100	-0.3650151669335209
2215	1	-0.8624875398974652
2215	2	-1.2264645034119006
2215	3	-0.9022491491569831
2215	4	-2.5260766539510016
2215	5	-1.1538625178280597
2215	6	-0.7796298815993268
2215	7	-0.801482904102528
2215	8	-0.743024356373013
2215	9	-1.0903267032882837
2215	10-11	-0.8087125984048384
2215	12-13	-0.9784954610055436
2215	14-15	-1.00939320934485
2215	16-17	-1.0366102182940296
2215	18-19	-0.9253602532257901
2215	20-21	-1.0522024407529145
2215	22-23	-1.0363934442733438
2215	24-25	-1.0924698560002
2215	26-27	-1.026018973625078
2215	28-29	-0.8736201474504384
2215	30-31	-3.7964547561048434
2215	32-33	-1.5560196219159366
2215	34-35	-1.3856053343495418
2215	36-37	-1.1604688056897245
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2215	42-43	-1.0048841812892206
2215	44-45	-0.9395498387686985
2215	46-47	-1.1633579931940332
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2215	58-59	-0.9884133468344487
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2215	62-63	-0.964343232169
2215	64-65	-0.943588975302724
2215	66-67	-0.9031578480732776
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2215	70-71	-1.0218430232022797
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2215	84-85	-0.8008133537920976
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2215	90-91	-0.8801184752876523
2215	92-93	-0.859098150299193
2215	94-95	-0.8918886378459696
2215	96-97	-1.1378425964088201
2215	98-99	-0.9647612488529411
2215	100	-0.5667884379557293
2216	1	-0.4926442730527718
2216	2	-0.7053999921476297
2216	3	-0.6920572976086561
2216	4	-0.45355863027588583
2216	5	-0.4964497871759477
2216	6	-0.4145166833556502
2216	7	-0.512266429825651
2216	8	-0.48583119769383387
2216	9	-0.7486787627582601
2216	10-11	-1.1113974958672799
2216	12-13	-0.6927316269833668
2216	14-15	-0.8328234080999124
2216	16-17	-0.7974877820861153
2216	18-19	-1.1803234070414987
2216	20-21	-1.2018867902269506
2216	22-23	-1.0190098220751906
2216	24-25	-1.0306482130492185
2216	26-27	-1.4784460293726767
2216	28-29	-1.3078579281617237
2216	30-31	-1.5900493485837686
2216	32-33	-1.7926440392838217
2216	34-35	-1.4296924933538762
2216	36-37	-1.582458577171245
2216	38-39	-1.3970912202891554
2216	40-41	-1.368948678381642
2216	42-43	-1.2923408888597763
2216	44-45	-1.2209108098578625
2216	46-47	-1.3701825385018793
2216	48-49	-1.287667155879312
2216	50-51	-1.3767001901149456
2216	52-53	-1.4311790596765874
2216	54-55	-1.4197254152227359
2216	56-57	-1.3843935259848124
2216	58-59	-1.2281657806560062
2216	60-61	-1.3319148486020467
2216	62-63	-1.3952865138178652
2216	64-65	-1.4099182226022862
2216	66-67	-1.3731679329487392
2216	68-69	-1.3651878861161677
2216	70-71	-1.2832132013321242
2216	72-73	-1.17236474929895
2216	74-75	-1.3162369366072042
2216	76-77	-1.3053803928975327
2216	78-79	-1.1315321203035182
2216	80-81	-1.2483396220556457
2216	82-83	-1.1853400722934282
2216	84-85	-1.2434661746097788
2216	86-87	-1.2437157805904455
2216	88-89	-1.2897175935000291
2216	90-91	-1.4104940374855133
2216	92-93	-1.3814233114521919
2216	94-95	-1.4261472618003097
2216	96-97	-1.3767322379540268
2216	98-99	-1.4307395608465434
2216	100	-1.0074451960690851
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	3.0
2	12.0
3	32.0
4	55.0
5	29.0
6	54.0
7	236.0
8	631.0
9	967.0
10	1675.0
11	3391.0
12	6076.0
13	8918.0
14	12392.0
15	15731.0
16	18846.0
17	22104.0
18	24634.0
19	27905.0
20	32163.0
21	36351.0
22	41926.0
23	48354.0
24	56361.0
25	64826.0
26	71824.0
27	78799.0
28	89675.0
29	106814.0
30	124968.0
31	143025.0
32	175570.0
33	227772.0
34	335800.0
35	476204.0
36	765617.0
37	1283813.0
38	1017163.0
39	410446.0
40	786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7030304109425	21.307472746655662	11.903857913406906	24.085638928994932
2	15.713982378456487	19.885049429408355	40.398013995272095	24.002954196863058
3	18.226597977032462	27.312172892137305	28.92468805183163	25.536541078998603
4	11.91327809821652	16.573867576000048	40.8641342448535	30.648720080929927
5	12.71866580340486	39.06519633012541	35.42180580482502	12.79433206164471
6	29.241753556122994	39.25790204968628	18.237033227235262	13.263311166955466
7	26.52858563478079	32.039253999546396	24.333065824595685	17.099094541077132
8	26.42042436130255	34.93298249905223	19.904826722071594	18.74176641757363
9	26.417638521476878	14.619719189540517	21.614404555271204	37.3482377337114
10-11	25.81131852950831	25.904114668742295	27.5981806792646	20.686386122484798
12-13	25.73883016427442	23.978987542247964	27.629675713236708	22.652506580240907
14-15	23.184277012044006	25.08391358351106	24.91206072779741	26.819748676647524
16-17	21.904120134422847	27.57938995056188	26.39068548237608	24.125804432639196
18-19	21.222251212283226	28.589864405895515	28.06102114160534	22.12686324021592
20-21	23.164925298662293	27.612217745903102	26.83835459565533	22.384502359779283
22-23	23.808589603102508	27.23159213124204	26.48981146335332	22.47000680230214
24-25	23.176515261017098	27.258085071805905	27.513891395582473	22.051508271594525
26-27	23.066218880721816	27.475120410296565	26.2743453948192	23.184315314162422
28-29	21.977197761648426	28.1662932475045	26.890270944941815	22.96623804590526
30-31	22.93862063773528	27.755704888381455	26.838489462399213	22.46718501148406
32-33	23.545934755231798	27.918249852658583	25.734229583225304	22.80158580888431
34-35	23.353850663149412	27.54243911970885	27.025231430595248	22.078478786546494
36-37	22.91884343226074	27.13537051694608	26.718059635256893	23.22772641553629
38-39	23.155676460894767	27.2782445586927	27.350179435907034	22.215899544505497
40-41	22.617142658743678	26.278098548736168	28.078218428200692	23.026540364319466
42-43	22.67025564913145	26.608062595074067	27.163466152323007	23.55821560347147
44-45	22.79107483409144	27.77953439334031	27.082326696641974	22.347064075926276
46-47	22.765485994096302	27.426637622516374	26.569377429791626	23.238498953595695
48-49	23.633229921318513	26.92592698908751	26.76531655776806	22.675526531825916
50-51	23.045189146480357	26.699559557152615	26.807676663543788	23.447574632823244
52-53	22.383076877973597	27.518736807277357	26.615148735732312	23.48303757901673
54-55	22.453948099123167	27.366674347400128	26.530541734258428	23.648835819218277
56-57	23.818787517331128	26.619127701221668	26.296296748675367	23.265788032771837
58-59	22.496338034509765	27.129028652802393	27.3605467092652	23.01408660342264
60-61	22.484694985922964	27.93321020107434	26.43567880801924	23.146416004983454
62-63	23.029877507036836	27.082008728260483	25.947477940309195	23.940635824393485
64-65	22.00872794045417	27.34207674452811	26.563607688306686	24.085587626711035
66-67	22.87627179847382	26.730248338606167	26.511669256189883	23.88181060673013
68-69	22.144080742176765	26.868882681033035	26.90108994366608	24.085946633124124
70-71	22.165646180938374	26.526972979301295	27.070529280609467	24.236851559150864
72-73	22.925930630323492	26.602398909313855	27.591147282758712	22.880523177603944
74-75	23.092914366773712	26.91403002314506	27.01258627052237	22.980469339558855
76-77	22.739275007099177	26.854015168467527	26.653851069500405	23.752858754932895
78-79	22.4837100010722	26.794656721571684	26.79309509412557	23.928538183230547
80-81	21.984806465596623	27.16283745828513	27.59727493817087	23.25508113794738
82-83	22.926302828908693	25.934560707898456	28.767901005599438	22.371235457593414
84-85	23.26871821562856	26.70812384390231	27.055122179618902	22.96803576085023
86-87	21.609873840311256	27.502592304445677	27.650723987055837	23.23680986818723
88-89	23.05367759524848	25.976880798196696	27.34870175964471	23.620739846910116
90-91	22.554922156206853	25.99928775289178	27.63986399255857	23.805926098342795
92-93	22.962658791474915	26.308987168266967	27.439100564328257	23.289253475929858
94-95	21.534390652789106	26.363958585757512	28.22521443533214	23.87643632612124
96-97	21.901467582970017	26.228937708965365	27.736162212737376	24.133432495327245
98-99	21.09861792737262	27.154973870222104	27.59924639598897	24.14716180641631
100	22.16209361370158	26.305924168336393	26.47055582424714	25.061426393714893
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	622.0
1	458.0
2	343.0
3	396.0
4	378.0
5	352.5
6	341.0
7	357.5
8	366.5
9	356.0
10	342.5
11	343.0
12	360.5
13	361.0
14	414.5
15	682.5
16	839.5
17	971.0
18	1304.0
19	1646.5
20	2106.0
21	2576.0
22	3382.0
23	4690.0
24	6389.0
25	8249.0
26	11491.0
27	15050.0
28	20506.0
29	33215.5
30	50885.0
31	67328.0
32	89880.5
33	110145.5
34	123231.0
35	140237.0
36	154559.0
37	182167.5
38	192295.0
39	173487.5
40	157160.0
41	140800.0
42	136883.0
43	146717.5
44	161801.0
45	198594.5
46	234383.5
47	259027.0
48	275894.5
49	270211.0
50	255759.5
51	263833.5
52	269761.5
53	256308.5
54	242407.5
55	207609.5
56	181477.0
57	149106.5
58	114116.5
59	111579.5
60	93044.5
61	61720.0
62	45167.5
63	38387.0
64	27987.0
65	12311.0
66	6064.0
67	3875.5
68	2544.5
69	1658.0
70	1049.0
71	654.0
72	375.5
73	222.0
74	123.5
75	78.5
76	68.0
77	48.5
78	41.0
79	33.0
80	24.5
81	25.5
82	21.0
83	14.5
84	16.0
85	16.0
86	10.5
87	11.0
88	11.5
89	12.5
90	13.0
91	11.0
92	9.0
93	8.0
94	8.5
95	6.5
96	5.0
97	6.0
98	6.0
99	9.0
100	15.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059054967002492
2	0.00769371948245169
3	0.008147317456473785
4	0.009944263276638238
5	0.0034717691088614203
6	0.00251223493304545
7	8.374116443484832E-4
8	0.001517808605381626
9	6.803969610331426E-4
10-11	4.972131638319119E-4
12-13	2.6169113885890103E-4
14-15	1.65737721277304E-4
16-17	6.193356952993992E-4
18-19	0.0017184384785067833
20-21	9.76980251739897E-4
22-23	0.0011339949350552378
24-25	0.0012648405044846881
26-27	0.0033409235394319696
28-29	0.004012597462503149
30-31	0.005347222270683544
32-33	0.0063154794844614785
34-35	0.003628783792176761
36-37	0.008539854164762136
38-39	0.006542278471472526
40-41	0.006236972142803807
42-43	0.015527007572294792
44-45	0.02147611946235381
46-47	0.019757680983847028
48-49	0.022880528574229914
50-51	0.01962683541441758
52-53	0.02644825110067293
54-55	0.020211278957869123
56-57	0.016224850609251863
58-59	0.018798146808031057
60-61	0.017149492633219982
62-63	0.01186333162827018
64-65	0.012194807070824789
66-67	0.014680872889984348
68-69	0.005608913409542445
70-71	0.00919408201190939
72-73	0.008897498721202636
74-75	0.012264591374520493
76-77	0.011348672388514341
78-79	0.013040941753135235
80-81	0.010964858718187953
82-83	0.006830138724217317
84-85	0.011383564540362195
86-87	0.009787248593322898
88-89	0.008347947329598943
90-91	0.013799846055826046
92-93	0.009281312391529023
94-95	0.0077024425204136535
96-97	0.010214677453459102
98-99	0.009700018213703265
100	0.2606967125312372
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5731948.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.95006947685731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.85280047244095	46.58941651796221
2	15.47540929063197	19.79306998637435
3	5.719259161669754	10.972410622348002
4	2.5775320652627087	6.593334186095108
5	1.3162247851131674	4.208633322757431
6	0.7557702620426501	2.8998936459702076
7	0.43076103351798906	1.9283038614978782
8	0.26961008444777884	1.3793266905677457
9	0.16753333718594943	0.9642391697458104
>10	0.43051597780789835	3.7202763320373506
>50	0.0025673847127057332	0.10637565875214075
>100	0.0016933178226546186	0.2007819916773756
>500	8.070686899227942E-5	0.027612472827217807
>1k	1.6141364989525746E-4	0.23346720147929437
>5k	8.070682494761973E-5	0.38285833990788454
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	9687	0.16900013747507828	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.7446075923926734E-5	0.0
14-15	0.0	0.0	0.0	1.7446075923926734E-5	0.0
16-17	0.0	0.0	0.0	1.7446075923926734E-5	0.0
18-19	0.0	0.0	0.0	2.61691138858901E-5	0.0
20-21	0.0	0.0	0.0	5.23382277717802E-5	0.0
22-23	0.0	0.0	0.0	1.1339949350552378E-4	0.0
24-25	0.0	0.0	0.0	2.2679898701104755E-4	0.0
26-27	0.0	0.0	0.0	6.455048091852892E-4	0.0
28-29	0.0	0.0	0.0	0.00344559999497553	0.0
30-31	0.0	0.0	0.0	0.012360544792102092	0.0
32-33	0.0	0.0	0.0	0.02836731945230487	0.0
34-35	0.0	0.0	0.0	0.05344605359294955	0.0
36-37	0.0	0.0	0.0	0.09558704998719458	0.0
38-39	0.0	0.0	0.0	0.15824463166797745	0.0
40-41	0.0	0.0	0.0	0.22706067814990644	0.0
42-43	0.0	0.0	0.0	0.30111054740901344	0.0
44-45	0.0	0.0	0.0	0.3898761817099527	0.0
46-47	0.0	0.0	0.0	0.5026388934442532	0.0
48-49	0.0	0.0	0.0	0.6334582937598178	0.0
50-51	0.0	0.0	0.0	0.773166469758623	0.0
52-53	0.0	0.0	0.0	0.9129182609472382	0.0
54-55	0.0	0.0	0.0	1.065597594395483	0.0
56-57	0.0	0.0	0.0	1.2509447050112805	0.0
58-59	0.0	0.0	0.0	1.473059420636754	0.0
60-61	0.0	0.0	0.0	1.703783774730685	0.0
62-63	0.0	0.0	0.0	1.9499827981691389	0.0
64-65	0.0	0.0	0.0	2.2101822975365444	0.0
66-67	0.0	0.0	0.0	2.5021249320475345	0.0
68-69	0.0	0.0	0.0	2.8335480363743706	0.0
70-71	0.0	0.0	0.0	3.196731721920715	1.7446075923926734E-5
72-73	0.0	0.0	0.0	3.581784063637702	1.7446075923926734E-5
74-75	0.0	0.0	0.0	3.957991244861258	1.7446075923926734E-5
76-77	0.0	0.0	0.0	4.3886040138535805	1.7446075923926734E-5
78-79	0.0	0.0	0.0	4.851474577229242	1.7446075923926734E-5
80-81	0.0	0.0	0.0	5.355735955734421	1.7446075923926734E-5
82-83	0.0	0.0	0.0	5.891330486598971	1.7446075923926734E-5
84-85	0.0	0.0	0.0	6.4042189496485316	1.7446075923926734E-5
86-87	0.0	0.0	0.0	6.952174025305184	1.7446075923926734E-5
88	0.0	0.0	0.0	7.396420902632054	1.7446075923926734E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6505	0.0	43.535397	94
GTACTAG	7490	0.0	34.026573	1
CAACGCA	10425	0.0	32.68201	5
GTATCAA	12435	0.0	32.179886	1
GCATCGT	7400	0.0	32.132378	8
TAGGCAT	8550	0.0	31.824327	5
TATTCTC	3240	0.0	31.61974	5
CTGTGCG	9585	0.0	30.935799	9
ACTGTGC	9985	0.0	30.543636	8
ATCAACG	11170	0.0	30.504366	3
TAGTACT	9985	0.0	30.31176	4
CTAGGCA	9680	0.0	29.082037	4
AGGGCTA	7570	0.0	29.053389	5
GGTATCA	8105	0.0	28.83395	1
GTACTGT	10670	0.0	28.583523	6
TACTGTG	11505	0.0	27.978977	7
CTAGTAC	11770	0.0	27.232346	3
CTATTGA	8000	0.0	27.19663	9
GCTATTG	8075	0.0	27.118614	8
TATCAAC	14645	0.0	26.989283	2
>>END_MODULE