FastQCFastQC Report
Mon 16 Jan 2017
ERR1146655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5731948
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156880.27369403909456264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132460.2310907216883335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82720.14431394004272197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC63100.044.4213194
GGTATCA119000.044.0018121
GTGGTAT23250.043.5048681
GTATCAA209050.040.575881
CAACGCA245350.033.1775635
ATCAACG247850.032.8540423
GTACTAG67300.032.1562271
TGAACCG8200.032.096445
CTGTGCG94000.032.006149
ACTGTGC97900.031.6851148
TATCAAC276750.030.6669982
TAGTACT100250.030.3599154
GAACCGT8050.029.7743156
AACGCAG287100.029.4159186
TACTGTG114100.029.244747
GTACTGT105350.029.130386
CTAACGC31550.028.776543
TAGGCAT87100.028.7062285
TCTAACG33500.028.227582
CGCCTAC32550.027.7220487
Produced by FastQC (version 0.11.5)