##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146655_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5731948
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14415709982016	33.0	31.0	34.0	30.0	34.0
2	32.19600387163317	33.0	31.0	34.0	30.0	34.0
3	32.36054985146411	34.0	31.0	34.0	30.0	34.0
4	35.96676225953201	37.0	35.0	37.0	35.0	37.0
5	35.877376766153496	37.0	35.0	37.0	35.0	37.0
6	35.91615433357037	37.0	35.0	37.0	35.0	37.0
7	35.83342242462772	37.0	35.0	37.0	35.0	37.0
8	35.84337802785371	37.0	35.0	37.0	35.0	37.0
9	37.547932046836436	39.0	37.0	39.0	35.0	39.0
10-11	37.483375721482474	39.0	37.0	39.0	34.5	39.0
12-13	37.44998114079193	39.0	37.0	39.0	34.0	39.0
14-15	38.661020302347474	40.0	38.0	41.0	34.0	41.0
16-17	38.66508942509597	40.0	38.0	41.0	34.0	41.0
18-19	38.723627290408075	40.0	38.0	41.0	34.0	41.0
20-21	38.72335870806923	40.0	38.0	41.0	34.0	41.0
22-23	38.68450996066259	40.0	38.0	41.0	34.0	41.0
24-25	38.600718725989836	40.0	38.0	41.0	34.0	41.0
26-27	38.45452209266378	40.0	38.0	41.0	34.0	41.0
28-29	38.39939659257202	40.0	38.0	41.0	34.0	41.0
30-31	38.257258178197006	40.0	37.5	41.0	34.0	41.0
32-33	38.106088977080745	40.0	37.0	41.0	33.0	41.0
34-35	38.00045909348793	40.0	37.0	41.0	33.0	41.0
36-37	37.98699892253035	40.0	37.0	41.0	33.0	41.0
38-39	37.883712046934136	40.0	37.0	41.0	33.0	41.0
40-41	37.75809297292997	40.0	37.0	41.0	33.0	41.0
42-43	37.61792744805082	40.0	36.0	41.0	32.5	41.0
44-45	37.38957427736609	40.0	36.0	41.0	32.0	41.0
46-47	37.11850220902213	40.0	35.5	41.0	31.0	41.0
48-49	36.81829894479154	39.0	35.0	41.0	30.5	41.0
50-51	36.59012067101796	39.0	35.0	41.0	30.0	41.0
52-53	36.395791535443095	39.0	35.0	41.0	30.0	41.0
54-55	36.20309151443803	38.5	35.0	41.0	30.0	41.0
56-57	35.957897297742406	38.0	35.0	40.0	29.5	41.0
58-59	35.65895521034036	37.0	35.0	40.0	29.0	41.0
60-61	35.47178149557533	37.0	35.0	40.0	29.0	41.0
62-63	35.250005233822776	36.5	34.0	40.0	29.0	41.0
64-65	34.997322899649475	36.0	34.0	39.0	29.0	41.0
66-67	34.75229372283209	35.0	34.0	39.0	29.0	41.0
68-69	34.51180872541063	35.0	34.0	39.0	28.5	41.0
70-71	34.314764195348594	35.0	34.0	38.0	28.5	41.0
72-73	34.095291164539525	35.0	34.0	37.0	28.0	40.0
74-75	33.852131945370054	35.0	34.0	37.0	28.0	39.5
76-77	33.62433408328199	35.0	33.5	36.0	28.0	39.0
78-79	33.41380844697126	35.0	33.0	36.0	27.0	39.0
80-81	33.2996973280288	35.0	33.0	36.0	27.5	38.5
82-83	33.14703256205395	35.0	33.0	35.5	27.0	37.0
84-85	33.02801107058194	35.0	33.0	35.0	28.0	37.0
86-87	32.89523518008188	35.0	33.0	35.0	27.0	36.5
88-89	32.796067235780924	35.0	33.0	35.0	27.0	36.0
90-91	32.73623522055678	35.0	33.0	35.0	27.0	36.0
92-93	32.66811797664599	35.0	33.0	35.0	27.0	36.0
94-95	32.6233024968126	35.0	33.0	35.0	27.0	35.5
96-97	32.53669249965282	35.0	33.0	35.0	27.0	35.0
98-99	32.46380942395151	35.0	33.0	35.0	27.0	35.0
100	31.213606438858132	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3767314028944071
1101	2	0.429156180856495
1101	3	0.36595528553355194
1101	4	0.2613484444027989
1101	5	0.29401310997749164
1101	6	0.3135223810390215
1101	7	0.32044523151355975
1101	8	0.3030552224431986
1101	9	0.3650007095879815
1101	10-11	0.3888507389873652
1101	12-13	0.36563139708912473
1101	14-15	0.5341518084997432
1101	16-17	0.537034078435795
1101	18-19	0.5702354196914001
1101	20-21	0.5382150768229792
1101	22-23	0.5020182592964701
1101	24-25	0.527477711302275
1101	26-27	0.5706239510705444
1101	28-29	0.5687288075484815
1101	30-31	0.6198739001141078
1101	32-33	0.6496984030833062
1101	34-35	0.6475152084133953
1101	36-37	0.6038447563015055
1101	38-39	0.622886640666863
1101	40-41	0.6370651827067917
1101	42-43	0.5995735524006278
1101	44-45	0.5974699788730362
1101	46-47	0.5818954151194475
1101	48-49	0.48952315474247143
1101	50-51	0.5291161121436403
1101	52-53	0.5849058338808959
1101	54-55	0.4899524915132858
1101	56-57	0.5270452780200685
1101	58-59	0.4460992092263112
1101	60-61	0.519904422394454
1101	62-63	0.518296446461818
1101	64-65	0.5169244035931655
1101	66-67	0.4772330904769433
1101	68-69	0.501592223153537
1101	70-71	0.4688745800250871
1101	72-73	0.4753458665228578
1101	74-75	0.49284352144976395
1101	76-77	0.4584733908254677
1101	78-79	0.4559943798731396
1101	80-81	0.4693960984663832
1101	82-83	0.41730274428532965
1101	84-85	0.358840739456717
1101	86-87	0.38791628657127575
1101	88-89	0.45611291494022765
1101	90-91	0.4619594548384498
1101	92-93	0.418209956537666
1101	94-95	0.4599560298102716
1101	96-97	0.40533010527911273
1101	98-99	0.43336455283380815
1101	100	0.7926169074554714
1102	1	0.35440506320155407
1102	2	0.39467799641411005
1102	3	0.29752527181294397
1102	4	0.19969857733388352
1102	5	0.25128108620868517
1102	6	0.2255561553045453
1102	7	0.2593914422653043
1102	8	0.2595343402531114
1102	9	0.31329092683767357
1102	10-11	0.3059314539041438
1102	12-13	0.3273057052571602
1102	14-15	0.42161458067255353
1102	16-17	0.4347122758048556
1102	18-19	0.4600050321148572
1102	20-21	0.44936413673764264
1102	22-23	0.5019741482654254
1102	24-25	0.4856198417009452
1102	26-27	0.49657301892442973
1102	28-29	0.4801827514080017
1102	30-31	0.5213484961298391
1102	32-33	0.539397122323912
1102	34-35	0.5157640831813239
1102	36-37	0.5332184789010128
1102	38-39	0.5366924773183896
1102	40-41	0.5177578085943537
1102	42-43	0.4929099876287921
1102	44-45	0.5463737809461833
1102	46-47	0.6045635612296394
1102	48-49	0.5422197039290069
1102	50-51	0.4612284974320531
1102	52-53	0.49468558153576936
1102	54-55	0.45953334892893594
1102	56-57	0.4476961873663612
1102	58-59	0.451737227515018
1102	60-61	0.4702607204369258
1102	62-63	0.4709573582102422
1102	64-65	0.46077262602167224
1102	66-67	0.4587879489755693
1102	68-69	0.45440266964318
1102	70-71	0.4141970676684039
1102	72-73	0.4244685951928062
1102	74-75	0.4530470632589285
1102	76-77	0.3945042911943304
1102	78-79	0.43654228035601506
1102	80-81	0.38949866711011794
1102	82-83	0.41579482643795984
1102	84-85	0.3668092884315399
1102	86-87	0.38513403449893246
1102	88-89	0.409955294915477
1102	90-91	0.3744020099855021
1102	92-93	0.4046425510144047
1102	94-95	0.3860852696602777
1102	96-97	0.4168359212683015
1102	98-99	0.41090663071148015
1102	100	0.56042417571161
1103	1	0.2746805059836319
1103	2	0.3414378647129226
1103	3	0.2865603301940354
1103	4	0.2264907020189355
1103	5	0.20064376244821602
1103	6	0.23442963446052545
1103	7	0.25859097848685053
1103	8	0.21789907525278096
1103	9	0.29701520162640804
1103	10-11	0.3333012577644965
1103	12-13	0.31228697574256614
1103	14-15	0.4055124007651685
1103	16-17	0.40695078725438805
1103	18-19	0.4086161122578602
1103	20-21	0.43848392387522495
1103	22-23	0.38008848749260693
1103	24-25	0.44631684720831544
1103	26-27	0.46917274244112406
1103	28-29	0.48831894173554957
1103	30-31	0.40257605973214794
1103	32-33	0.4413730117900414
1103	34-35	0.4595294588493104
1103	36-37	0.4498730680037042
1103	38-39	0.4540059439880295
1103	40-41	0.4930380080785426
1103	42-43	0.38696578225818
1103	44-45	0.5335537255008731
1103	46-47	0.5284815973062535
1103	48-49	0.5696721926504296
1103	50-51	0.5562729800292132
1103	52-53	0.5083609143191055
1103	54-55	0.46684046094183174
1103	56-57	0.4769642126660756
1103	58-59	0.5779873482906694
1103	60-61	0.49490452520403494
1103	62-63	0.5546175290055828
1103	64-65	0.549790775813193
1103	66-67	0.4842652914655545
1103	68-69	0.49736646668064566
1103	70-71	0.5297325311124013
1103	72-73	0.4848006724594853
1103	74-75	0.4628969670100318
1103	76-77	0.40764672469341434
1103	78-79	0.4446563594510522
1103	80-81	0.3712188095138842
1103	82-83	0.32514490484922476
1103	84-85	0.3674703957026111
1103	86-87	0.37936671898836494
1103	88-89	0.3875393361798629
1103	90-91	0.35477113750228284
1103	92-93	0.32472605343363625
1103	94-95	0.321344324117085
1103	96-97	0.3969647981663371
1103	98-99	0.3924329746656312
1103	100	0.5746322219705391
1104	1	0.22248261603382247
1104	2	0.2374530003279247
1104	3	0.20088721659742959
1104	4	0.17949715040739989
1104	5	0.15547259100110722
1104	6	0.1786476588816086
1104	7	0.22939278637898752
1104	8	0.19815451671104967
1104	9	0.2275536215220697
1104	10-11	0.27214755970718585
1104	12-13	0.30855731094354155
1104	14-15	0.3250107600444707
1104	16-17	0.3462109096101642
1104	18-19	0.35428362632838173
1104	20-21	0.39891145200594025
1104	22-23	0.3848979541070605
1104	24-25	0.38655347124468875
1104	26-27	0.3960657152850544
1104	28-29	0.4046257534672506
1104	30-31	0.38541797296878855
1104	32-33	0.3926099029798351
1104	34-35	0.47197775104854855
1104	36-37	0.41622242616699623
1104	38-39	0.41698108632197517
1104	40-41	0.48443792241442907
1104	42-43	0.5114376741444175
1104	44-45	0.47201427758716363
1104	46-47	0.46290430403475114
1104	48-49	0.4237902448078188
1104	50-51	0.41791569161460984
1104	52-53	0.3979900074000753
1104	54-55	0.4270858069662893
1104	56-57	0.35603705050394296
1104	58-59	0.4344953114803687
1104	60-61	0.38989429242565166
1104	62-63	0.4235410647848141
1104	64-65	0.43822049829693555
1104	66-67	0.35168245506433493
1104	68-69	0.35366659593039884
1104	70-71	0.2906715766186707
1104	72-73	0.3161659917637465
1104	74-75	0.362469041430856
1104	76-77	0.2916630069532786
1104	78-79	0.3275296431604531
1104	80-81	0.27753033957861106
1104	82-83	0.311989282023184
1104	84-85	0.31436505922177105
1104	86-87	0.3526426313513511
1104	88-89	0.3470047716557474
1104	90-91	0.3171054762465104
1104	92-93	0.3211196098096991
1104	94-95	0.2954676464166681
1104	96-97	0.26844901411478617
1104	98-99	0.291854541312631
1104	100	0.5051518025663988
1105	1	0.23484908878918986
1105	2	0.19868581269162178
1105	3	0.16152119015244182
1105	4	0.1918466450034657
1105	5	0.18588895142323025
1105	6	0.13313259748476014
1105	7	0.2249443771225117
1105	8	0.2048843960140374
1105	9	0.18847834007679864
1105	10-11	0.1916894856215734
1105	12-13	0.179260067087057
1105	14-15	0.33413206688830144
1105	16-17	0.36089440491673486
1105	18-19	0.28586964323116604
1105	20-21	0.3364687532935662
1105	22-23	0.3302411582711997
1105	24-25	0.30306089792198776
1105	26-27	0.3658176777689661
1105	28-29	0.35152496408327494
1105	30-31	0.3652548484881706
1105	32-33	0.34817360632481353
1105	34-35	0.2840308787220138
1105	36-37	0.33762265346389597
1105	38-39	0.3567074191983508
1105	40-41	0.3936414047448977
1105	42-43	0.3682916745526654
1105	44-45	0.3278529661348415
1105	46-47	0.35058764663352804
1105	48-49	0.3564525574454649
1105	50-51	0.3284288775226756
1105	52-53	0.3483107116770867
1105	54-55	0.36599378060815724
1105	56-57	0.28468139804906656
1105	58-59	0.30763607749474176
1105	60-61	0.2651534903314854
1105	62-63	0.25548705333837063
1105	64-65	0.24255484062712895
1105	66-67	0.2683579416057711
1105	68-69	0.24133710327988211
1105	70-71	0.30271251110421105
1105	72-73	0.25212730422997964
1105	74-75	0.251430313340812
1105	76-77	0.2122509967181756
1105	78-79	0.20311809181390572
1105	80-81	0.19781301540921703
1105	82-83	0.21570043742949707
1105	84-85	0.21120018565596865
1105	86-87	0.18245708374401204
1105	88-89	0.23985812517220495
1105	90-91	0.2238408304080437
1105	92-93	0.2194451017841459
1105	94-95	0.12460673274109269
1105	96-97	0.21093995925591003
1105	98-99	0.20347186952809437
1105	100	0.37067070820112136
1106	1	0.16214265199770495
1106	2	0.20644348678496272
1106	3	0.15195579473112986
1106	4	0.13677958103428267
1106	5	0.10479376751489866
1106	6	0.16663889441266377
1106	7	0.18377932277201836
1106	8	0.0983818134454566
1106	9	0.13462243022745923
1106	10-11	0.17743666687985638
1106	12-13	0.14799166398551478
1106	14-15	0.19180650528699061
1106	16-17	0.16862742652197937
1106	18-19	0.18755682424406928
1106	20-21	0.22296973325872216
1106	22-23	0.26573637039761167
1106	24-25	0.23619667907231445
1106	26-27	0.26372072102852684
1106	28-29	0.2376709175479661
1106	30-31	0.27838626656983934
1106	32-33	0.3174551875806415
1106	34-35	0.33242298440489293
1106	36-37	0.22267553937693663
1106	38-39	0.28022048693402724
1106	40-41	0.2711250566563663
1106	42-43	0.2387708376409492
1106	44-45	0.18195394395296205
1106	46-47	0.25806797065080644
1106	48-49	0.24928938021132296
1106	50-51	0.25001038639297235
1106	52-53	0.15733118359394638
1106	54-55	0.1294155842961331
1106	56-57	0.14428682021621597
1106	58-59	0.1371852091750725
1106	60-61	0.1590218452782466
1106	62-63	0.17044804643107625
1106	64-65	0.13943766194528706
1106	66-67	0.18624368594988283
1106	68-69	0.12561245815302158
1106	70-71	0.21904050958288224
1106	72-73	0.13122697849507858
1106	74-75	0.13193552448573342
1106	76-77	0.1952072326091283
1106	78-79	0.17169019516572348
1106	80-81	0.12244119607860426
1106	82-83	0.12895320096190943
1106	84-85	0.13032411367530727
1106	86-87	0.196984607115958
1106	88-89	0.2346375870041726
1106	90-91	0.14025566852208726
1106	92-93	0.1931888600655398
1106	94-95	0.22743827995117982
1106	96-97	0.1601186441702751
1106	98-99	0.15179270153231528
1106	100	0.2770943874718661
1107	1	0.1250356466008924
1107	2	0.17195084240678682
1107	3	0.18954204361800464
1107	4	0.11628491808103547
1107	5	0.01849557422176673
1107	6	0.04611550673269704
1107	7	0.09880269102824712
1107	8	0.12005385401418067
1107	9	0.1097871211508945
1107	10-11	0.1745105921492538
1107	12-13	0.048425747274521314
1107	14-15	0.16428913224355313
1107	16-17	0.13791522138932066
1107	18-19	0.1299131333744299
1107	20-21	0.1393002820242728
1107	22-23	0.08425810407121048
1107	24-25	0.08557564226282466
1107	26-27	0.14911569162396887
1107	28-29	0.18508104165074002
1107	30-31	0.12281245560169651
1107	32-33	0.18221682090820224
1107	34-35	0.18916410626048474
1107	36-37	0.09465141210061745
1107	38-39	0.16798636808463385
1107	40-41	0.22852290644808448
1107	42-43	0.2654241342982715
1107	44-45	0.18038388923777404
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2216	76-77	-0.915964079301169
2216	78-79	-0.9283369339775263
2216	80-81	-0.8945820019567776
2216	82-83	-0.8022289470922388
2216	84-85	-0.8182242250670484
2216	86-87	-0.850846554593133
2216	88-89	-0.8251138177385968
2216	90-91	-0.7935282787818245
2216	92-93	-0.7629515180282311
2216	94-95	-0.8387194033945065
2216	96-97	-0.8382670845429452
2216	98-99	-0.8277237287611072
2216	100	-0.39168214052525485
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	16.0
13	77.0
14	336.0
15	959.0
16	2395.0
17	5030.0
18	8926.0
19	14046.0
20	20105.0
21	28719.0
22	40141.0
23	47590.0
24	48681.0
25	46867.0
26	47844.0
27	55430.0
28	69244.0
29	86895.0
30	108074.0
31	135918.0
32	171154.0
33	221423.0
34	317815.0
35	466355.0
36	747456.0
37	1406431.0
38	1151234.0
39	481383.0
40	1397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.826756976860224	22.200463088639324	13.106085400635175	24.866694533865275
2	14.450008601548697	20.695622771317794	39.84577580211622	25.008592825017285
3	17.01278518228009	27.61626588377982	29.742331926249154	25.62861700769093
4	11.52227086557857	17.308271217598776	39.63157313918209	31.537884777640567
5	12.19482280247556	39.1484148450611	35.45332275126921	13.203439601194129
6	29.24006450164316	39.62341242862155	17.570295049832108	13.566228019903184
7	25.633632975607235	32.776058951008956	24.340093224883834	17.25021484849997
8	26.75280724807692	34.4373326485167	19.56900167272976	19.24085843067662
9	26.48596951682046	13.907837265795154	21.724446907054983	37.8817463103294
10-11	25.726977983750025	25.960406479612168	27.370380889708002	20.942234646929805
12-13	25.95743040172675	23.895097481110643	28.148288944859946	21.999183172302665
14-15	23.219243218552286	24.723337207611312	25.901262529572882	26.156157044263516
16-17	21.30820970462398	27.025943012741916	27.454750112876113	24.211097169757995
18-19	20.688829190308663	27.16393662987083	30.353962840003568	21.79327133981694
20-21	22.363932820046518	26.392458549868213	29.418157666468716	21.825450963616557
22-23	22.89881206179819	25.950409878107756	29.151005905845622	21.999772154248433
24-25	22.31169926873028	26.068781503251596	30.118233801144044	21.501285426874077
26-27	22.28815578927094	26.27788144623782	28.88330459383093	22.550658170660306
28-29	21.31870351929222	26.93427260679964	29.457236876538307	22.289786997369827
30-31	22.34434087678395	26.46012315533916	29.363446772371276	21.83208919550561
32-33	23.02427551680511	26.66725169174598	28.3686191849612	21.93985360648771
34-35	22.685001678312506	26.471000783677727	29.368942286287314	21.475055251722452
36-37	22.364909800298257	25.969984375294402	29.05506993433995	22.61003589006739
38-39	22.559930760013874	25.974450570730927	29.84844768305644	21.617170986198758
40-41	21.949257041410704	25.294358916026454	30.307000342640933	22.449383699921913
42-43	22.302574971022068	25.4883854494144	29.218696680430455	22.990342899133072
44-45	22.41894029743466	26.629751351547505	28.948099319812393	22.003209031205447
46-47	22.518173577289954	26.40314427137162	28.131596797458734	22.947085353879693
48-49	23.600824710901076	26.168921979054936	27.941460739001815	22.288792571042165
50-51	22.782350781968013	26.11592080039805	27.74370946840411	23.358018949229827
52-53	21.943456221165995	26.965928511563607	27.51521821202844	23.575397055241954
54-55	22.167568512484763	26.88888664028355	27.24737733140636	23.696167515825337
56-57	23.50379835965016	26.122026926971426	27.04296165980571	23.33121305357271
58-59	22.111066458837325	26.63820222327616	28.121380756083468	23.129350561803044
60-61	22.277723035868433	27.422501041530733	27.1277932039858	23.171982718615034
62-63	22.87064537221901	26.476225883416948	26.589660269074315	24.063468475289724
64-65	21.8346545688545	26.753761004880456	27.178686538453654	24.23289788781139
66-67	22.5682567471673	26.205845575038477	27.20489607144524	24.02100160634898
68-69	22.06497686301411	26.09771582017143	27.67612337027482	24.16118394653964
70-71	22.045276733650812	25.811025032848896	27.71794867992509	24.42574955357521
72-73	22.758399003477013	25.778969736197055	28.177734201220183	23.284897059105745
74-75	22.885657590714743	26.297391591374904	27.531933431078293	23.285017386832067
76-77	22.56794468611162	26.294369342885883	27.025683442016685	24.112002528985812
78-79	22.16674562471928	26.317883462450318	27.331300108959493	24.184070803870902
80-81	21.74718786702182	26.832169447454863	27.95038440683691	23.47025827868641
82-83	22.72093187167783	25.521794684808725	29.12535145119949	22.63192199231396
84-85	23.12700673488315	26.291751076597347	27.28694503160182	23.294297156917683
86-87	21.38983117083407	27.056709167633763	27.90523396234579	23.648225699186384
88-89	22.545895391933072	25.68078077470347	27.64307177943694	24.13025205392652
90-91	22.05107234050274	25.596027737865036	28.01316411104916	24.33973581058307
92-93	22.572160459236546	25.99399889880369	27.480823273344416	23.95301736861535
94-95	21.157187748388505	26.046459249106935	28.280123964837085	24.516229037667472
96-97	21.54576419744213	25.841616148646153	27.785789403532625	24.826830250379103
98-99	20.752037527207158	26.7368964268343	27.726900174251405	24.78416587170714
100	21.729002839453475	25.953180877463573	26.54860196413717	25.76921431894578
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	448.0
1	304.0
2	231.0
3	314.0
4	244.0
5	189.5
6	366.0
7	773.0
8	1304.5
9	1507.5
10	1187.0
11	933.0
12	971.5
13	1084.5
14	1344.0
15	1963.5
16	2699.5
17	3596.5
18	4874.5
19	6302.0
20	7966.5
21	9592.5
22	11363.0
23	13443.0
24	15365.5
25	17272.0
26	20053.0
27	22685.0
28	27305.0
29	39052.0
30	55221.0
31	68935.5
32	89527.0
33	108820.0
34	119425.5
35	134987.5
36	148884.5
37	176263.5
38	186621.0
39	167656.0
40	152480.5
41	137336.5
42	133102.0
43	141695.5
44	155909.0
45	193530.0
46	230563.5
47	255167.5
48	273605.5
49	267434.5
50	252219.5
51	258770.5
52	263888.5
53	252890.0
54	244779.0
55	210543.5
56	180269.5
57	148393.0
58	113764.0
59	112957.0
60	93610.5
61	60173.0
62	42970.0
63	35765.0
64	25701.0
65	10759.0
66	4972.5
67	3155.5
68	2018.5
69	1224.5
70	713.0
71	403.0
72	193.0
73	80.0
74	25.5
75	15.5
76	7.0
77	3.5
78	2.0
79	2.0
80	1.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007362244039897083
3	0.0
4	0.08730016392332937
5	0.004134719993970636
6	1.7446075923926734E-5
7	3.489215184785347E-5
8	0.0
9	0.0
10-11	0.0
12-13	2.965832907067545E-4
14-15	0.009726187327589154
16-17	0.0
18-19	7.850734165767031E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.61691138858901E-5
60-61	0.0
62-63	0.0
64-65	4.361518980981684E-5
66-67	9.595341758159703E-5
68-69	0.0
70-71	3.401984805165713E-4
72-73	3.1402936663068123E-4
74-75	4.187058221742416E-4
76-77	1.046764555435604E-4
78-79	2.61691138858901E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0651262014240185
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5731948.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.463233616154575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.28321160524294	42.4955024258143
2	16.85655400714593	20.384035257947822
3	6.177383328576558	11.205137139967889
4	2.822584481848007	6.826503397092345
5	1.5200549073077974	4.595371748496676
6	0.8315765740124381	3.0167885198541304
7	0.4853062055456316	2.054022773689254
8	0.30099012788792673	1.4559069134915164
9	0.19921934738128824	1.0840901347415413
>10	0.5111514393448874	4.224141920714472
>50	0.0054007413631645546	0.22276469742440855
>100	0.005227738133100823	0.6482275501670023
>500	6.481433485883997E-4	0.27251920869049523
>1k	5.61724200153213E-4	0.5429483185844791
>5k	4.320955385793588E-5	0.21611339097924037
>10k+	8.641910771587176E-5	0.7559266023444561
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15688	0.27369403909456264	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13246	0.2310907216883335	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8272	0.14431394004272197	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.7446075923926734E-5	0.0
14-15	0.0	0.0	0.0	1.7446075923926734E-5	0.0
16-17	0.0	0.0	0.0	1.7446075923926734E-5	0.0
18-19	0.0	0.0	0.0	2.61691138858901E-5	0.0
20-21	0.0	0.0	0.0	3.489215184785347E-5	0.0
22-23	0.0	0.0	0.0	8.723037961963368E-5	0.0
24-25	0.0	0.0	0.0	2.093529110871208E-4	0.0
26-27	0.0	0.0	0.0	6.280587332613625E-4	0.0
28-29	0.0	0.0	0.0	0.0034543230329374935	0.0
30-31	0.0	0.0	0.0	0.012465221247645653	0.0
32-33	0.0	0.0	0.0	0.028585395401353957	0.0
34-35	0.0	0.0	0.0	0.05394326675678146	0.0
36-37	0.0	0.0	0.0	0.09633723125192342	0.0
38-39	0.0	0.0	0.0	0.15942224179284248	0.0
40-41	0.0	0.0	0.0	0.22886634700803288	0.0
42-43	0.0	0.0	0.0	0.3032738608235804	0.0
44-45	0.0	0.0	0.0	0.39277223031332453	0.0
46-47	0.0	0.0	0.0	0.5060583243253428	0.0
48-49	0.0	0.0	0.0	0.6375406755260167	0.0
50-51	0.0	0.0	0.0	0.7779205254478931	0.0
52-53	0.0	0.0	0.0	0.918675466002134	0.0
54-55	0.0	0.0	0.0	1.0729075002076083	0.0
56-57	0.0	0.0	0.0	1.259868372846369	0.0
58-59	0.0	0.0	0.0	1.484233632266029	0.0
60-61	0.0	0.0	0.0	1.7168683316736302	0.0
62-63	0.0	0.0	0.0	1.9655185287793957	0.0
64-65	0.0	0.0	0.0	2.228116863586341	0.0
66-67	0.0	0.0	0.0	2.5223711031572513	0.0
68-69	0.0	0.0	0.0	2.8561843198856653	0.0
70-71	0.0	0.0	0.0	3.2223600074529637	0.0
72-73	0.0	0.0	0.0	3.6100903218242735	0.0
74-75	0.0	0.0	0.0	3.9894029045622887	0.0
76-77	0.0	0.0	0.0	4.424682498864261	0.0
78-79	0.0	0.0	0.0	4.892455409574547	0.0
80-81	0.0	0.0	0.0	5.402247194147609	0.0
82-83	0.0	0.0	0.0	5.944802709305806	0.0
84-85	0.0	0.0	0.0	6.464756833104557	0.0
86-87	0.0	0.0	0.0	7.019699062168742	0.0
88	0.0	0.0	0.0	7.470915646827222	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6310	0.0	44.42131	94
GGTATCA	11900	0.0	44.001812	1
GTGGTAT	2325	0.0	43.504868	1
GTATCAA	20905	0.0	40.57588	1
CAACGCA	24535	0.0	33.177563	5
ATCAACG	24785	0.0	32.854042	3
GTACTAG	6730	0.0	32.156227	1
TGAACCG	820	0.0	32.09644	5
CTGTGCG	9400	0.0	32.00614	9
ACTGTGC	9790	0.0	31.685114	8
TATCAAC	27675	0.0	30.666998	2
TAGTACT	10025	0.0	30.359915	4
GAACCGT	805	0.0	29.774315	6
AACGCAG	28710	0.0	29.415918	6
TACTGTG	11410	0.0	29.24474	7
GTACTGT	10535	0.0	29.13038	6
CTAACGC	3155	0.0	28.77654	3
TAGGCAT	8710	0.0	28.706228	5
TCTAACG	3350	0.0	28.22758	2
CGCCTAC	3255	0.0	27.722048	7
>>END_MODULE