FastQCFastQC Report
Mon 16 Jan 2017
ERR1146654_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146654_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6147020
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC63400.042.65023894
GTACTAG65700.035.206651
CAACGCA104100.033.6777845
GCATCGT67750.032.2538348
GTATCAA122450.031.8672281
CTGTGCG90200.031.7284269
TAGGCAT82350.031.2161315
TAGTACT95650.031.102254
ATCAACG114350.030.865753
CTAGGCA92200.028.5959824
ACTGTGC103450.028.5731358
GTACTGT104700.027.9631546
CTAGTAC109350.027.6783473
AACGCAG136600.027.4534536
TATCAAC141300.027.3735262
TACTGTG114950.027.1863177
GGTATCA78700.026.8820761
ACGCAGA129600.026.8327037
AGGGCTA79900.026.5856385
CGCAGAG130550.026.385478
Produced by FastQC (version 0.11.5)