##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146654_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6147020
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.062010697866608	31.0	31.0	34.0	28.0	34.0
2	31.0769081603769	31.0	31.0	34.0	27.0	34.0
3	31.245395817810905	31.0	31.0	34.0	27.0	34.0
4	34.697225810229995	37.0	35.0	37.0	31.0	37.0
5	34.87775328533176	37.0	35.0	37.0	32.0	37.0
6	34.92152457613608	37.0	35.0	37.0	32.0	37.0
7	34.86009236996138	37.0	35.0	37.0	31.0	37.0
8	34.85779743680678	37.0	35.0	37.0	31.0	37.0
9	36.309688109034944	39.0	37.0	39.0	31.0	39.0
10-11	36.13062272450716	39.0	37.0	39.0	30.0	39.0
12-13	36.04395804796471	39.0	35.0	39.0	30.0	39.0
14-15	37.150761588542096	40.0	37.0	41.0	30.0	41.0
16-17	37.16760649875875	40.0	36.5	41.0	30.0	41.0
18-19	37.12623791690933	40.0	36.5	41.0	30.0	41.0
20-21	37.20509075942489	40.0	37.0	41.0	30.0	41.0
22-23	37.1370821145856	40.0	36.5	41.0	30.0	41.0
24-25	37.08984686888931	40.0	36.0	41.0	30.0	41.0
26-27	37.13587095861084	40.0	36.0	41.0	30.0	41.0
28-29	37.12427761744715	40.0	36.0	41.0	30.0	41.0
30-31	36.989410885274495	40.0	36.0	41.0	30.0	41.0
32-33	36.98932336319062	40.0	36.0	41.0	30.0	41.0
34-35	36.92583040237383	40.0	36.0	41.0	30.0	41.0
36-37	36.80140311890965	40.0	35.5	41.0	30.0	41.0
38-39	36.69297155369594	40.0	35.0	41.0	29.5	41.0
40-41	36.61330555293459	39.0	35.0	41.0	29.0	41.0
42-43	36.44048872461778	39.0	35.0	41.0	28.5	41.0
44-45	36.280839577551404	39.0	35.0	41.0	28.0	41.0
46-47	36.28843244368816	39.0	35.0	41.0	28.0	41.0
48-49	36.15854527885057	39.0	35.0	41.0	28.0	41.0
50-51	35.978311441967	39.0	34.5	40.0	28.0	41.0
52-53	35.77296657567406	38.0	34.0	40.0	28.0	41.0
54-55	35.57309436117013	38.0	34.0	40.0	27.5	41.0
56-57	35.337094722320735	37.5	34.0	40.0	27.0	41.0
58-59	35.04212073492522	37.0	33.0	40.0	26.5	41.0
60-61	34.79868684338102	36.5	33.0	40.0	26.0	41.0
62-63	34.5034732276778	36.0	33.0	39.5	26.0	41.0
64-65	34.356496969263155	35.5	33.0	39.0	26.0	41.0
66-67	34.10270334243259	35.0	33.0	39.0	26.0	41.0
68-69	33.985585942456666	35.0	33.0	39.0	26.0	41.0
70-71	33.91603842186946	35.0	33.0	38.5	26.0	41.0
72-73	33.70464696714831	35.0	33.0	37.5	26.0	40.0
74-75	33.45454415310183	35.0	33.0	37.0	26.0	40.0
76-77	33.18809976216117	35.0	32.5	36.5	25.5	39.0
78-79	32.93865930158027	35.0	32.0	36.0	25.0	39.0
80-81	32.74642721188478	35.0	32.0	36.0	25.0	39.0
82-83	32.49011651174065	35.0	32.0	35.5	25.0	37.0
84-85	32.3067797892312	35.0	32.0	35.0	25.0	37.0
86-87	32.12464966764384	35.0	32.0	35.0	24.5	36.5
88-89	31.99025479012595	35.0	32.0	35.0	24.0	36.0
90-91	31.83497930704634	35.0	32.0	35.0	24.0	36.0
92-93	31.681030645743792	35.0	31.0	35.0	24.0	36.0
94-95	31.60940837999551	35.0	31.0	35.0	24.0	35.5
96-97	31.502774596471134	35.0	31.0	35.0	23.0	35.0
98-99	31.41225471854655	35.0	31.0	35.0	23.0	35.0
100	30.427017156280606	34.0	29.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4573630972135412
1101	2	0.4854892576495473
1101	3	0.5406619056296975
1101	4	0.6511333540029511
1101	5	0.5231996232098552
1101	6	0.5063981986527253
1101	7	0.5241031994076835
1101	8	0.548259371476135
1101	9	0.7537416660123526
1101	10-11	0.7590842739866588
1101	12-13	0.7063572973339447
1101	14-15	0.9334380913801255
1101	16-17	0.859580157243002
1101	18-19	0.8617726005337403
1101	20-21	0.8304434948089536
1101	22-23	0.851179969438725
1101	24-25	0.8546721277004181
1101	26-27	0.8130708297241114
1101	28-29	0.8101427088512025
1101	30-31	0.8665666473925171
1101	32-33	0.8589722230532644
1101	34-35	0.931375582829908
1101	36-37	0.8956204272265893
1101	38-39	0.8637161114107315
1101	40-41	0.7993161072014416
1101	42-43	0.8373584206371874
1101	44-45	0.8313528377692379
1101	46-47	0.8100427306192799
1101	48-49	0.7816865740719408
1101	50-51	0.7712936600156723
1101	52-53	0.7569151538593388
1101	54-55	0.8072637937209493
1101	56-57	0.7286949490353436
1101	58-59	0.7370241680822787
1101	60-61	0.7229530071170842
1101	62-63	0.6984530053304709
1101	64-65	0.7503281036467868
1101	66-67	0.7088184498664063
1101	68-69	0.7331099498633975
1101	70-71	0.7166661471926119
1101	72-73	0.6887526154623416
1101	74-75	0.6349420232534868
1101	76-77	0.6620480398512996
1101	78-79	0.6812413309305967
1101	80-81	0.6443158374312645
1101	82-83	0.6338406688826623
1101	84-85	0.6508460183511531
1101	86-87	0.6607736271370683
1101	88-89	0.6860464170378222
1101	90-91	0.6524852189303658
1101	92-93	0.6401955051768979
1101	94-95	0.6620623878879499
1101	96-97	0.6257622487697034
1101	98-99	0.6251870080335884
1101	100	0.7629712393753287
1102	1	0.3543361503314948
1102	2	0.46948648632528744
1102	3	0.44382477777967466
1102	4	0.5560756323281097
1102	5	0.4485251665894978
1102	6	0.45731335211004165
1102	7	0.47884890455354423
1102	8	0.46983963789049454
1102	9	0.6125286355064503
1102	10-11	0.7674896373567179
1102	12-13	0.6301489034239367
1102	14-15	0.7960689389101105
1102	16-17	0.7973728026143405
1102	18-19	0.800715116821948
1102	20-21	0.7810089663020747
1102	22-23	0.7920775494921983
1102	24-25	0.8073388879121381
1102	26-27	0.7791201963525367
1102	28-29	0.797248173390301
1102	30-31	0.8195662709188625
1102	32-33	0.7610032244113327
1102	34-35	0.7821814602390518
1102	36-37	0.8380355602860803
1102	38-39	0.8205262536680067
1102	40-41	0.7419545391083915
1102	42-43	0.7444451984056286
1102	44-45	0.7599835237845767
1102	46-47	0.771704792659385
1102	48-49	0.6960392169524496
1102	50-51	0.7136116688487988
1102	52-53	0.703925079434029
1102	54-55	0.7139232422680024
1102	56-57	0.6636798876566488
1102	58-59	0.6384906848266709
1102	60-61	0.6525278830847583
1102	62-63	0.7189992444893036
1102	64-65	0.6798255929768402
1102	66-67	0.7072176375951358
1102	68-69	0.6625629079962891
1102	70-71	0.5953264187415286
1102	72-73	0.5917288318763809
1102	74-75	0.5578108796944292
1102	76-77	0.5751219614996046
1102	78-79	0.5677696551696911
1102	80-81	0.580992162311702
1102	82-83	0.6298513825014425
1102	84-85	0.5959441082599071
1102	86-87	0.5900479739914246
1102	88-89	0.5487299618448276
1102	90-91	0.4976674993849599
1102	92-93	0.5407075114339861
1102	94-95	0.5977044005687944
1102	96-97	0.5999785466110268
1102	98-99	0.5518903949551692
1102	100	0.5797928982376561
1103	1	0.29409656058779277
1103	2	0.34588267216882684
1103	3	0.3406840679918126
1103	4	0.4292498139567087
1103	5	0.28652026207253556
1103	6	0.31249464077881584
1103	7	0.3195592633395776
1103	8	0.31966682813323644
1103	9	0.4818233343167506
1103	10-11	0.6079593711506988
1103	12-13	0.5353950434217154
1103	14-15	0.6620628190356683
1103	16-17	0.6266472130546745
1103	18-19	0.6915452896593592
1103	20-21	0.5343791881079412
1103	22-23	0.6141457660697185
1103	24-25	0.6207781354930972
1103	26-27	0.5837698241069305
1103	28-29	0.5357975170245481
1103	30-31	0.5478600656598402
1103	32-33	0.5513284895426338
1103	34-35	0.6152584123769032
1103	36-37	0.5903135192509055
1103	38-39	0.6575556265764604
1103	40-41	0.550349671772949
1103	42-43	0.5052570539038683
1103	44-45	0.4787305088634852
1103	46-47	0.43171654301814044
1103	48-49	0.44935462099748946
1103	50-51	0.39791163466910007
1103	52-53	0.4090829913831442
1103	54-55	0.3605659041970597
1103	56-57	0.4000698954627211
1103	58-59	0.3383579022936374
1103	60-61	0.3627042183064688
1103	62-63	0.3441790915713909
1103	64-65	0.31881061738011596
1103	66-67	0.38946818188108523
1103	68-69	0.3628762836057504
1103	70-71	0.39499820130136953
1103	72-73	0.3198413577971806
1103	74-75	0.3014388056370194
1103	76-77	0.2694727570774873
1103	78-79	0.3744148345230158
1103	80-81	0.32852295448276436
1103	82-83	0.38935803781917855
1103	84-85	0.40977533182310566
1103	86-87	0.36124074780624227
1103	88-89	0.437156572125474
1103	90-91	0.4724616551247607
1103	92-93	0.31405964337877634
1103	94-95	0.3005001532048297
1103	96-97	0.30599725128741184
1103	98-99	0.3384186715307109
1103	100	0.4776740111372213
1104	1	0.30927702351353403
1104	2	0.40208808945757823
1104	3	0.39240993210739106
1104	4	0.5420870537646252
1104	5	0.43419491292738854
1104	6	0.39569351261899044
1104	7	0.4137587568765113
1104	8	0.5080718747360393
1104	9	0.5244199339624132
1104	10-11	0.66495903983094
1104	12-13	0.5672137718381904
1104	14-15	0.6629315753379785
1104	16-17	0.6725369537786747
1104	18-19	0.5998094149836035
1104	20-21	0.603739924618246
1104	22-23	0.6604750955938741
1104	24-25	0.6850841985660452
1104	26-27	0.6157612818524996
1104	28-29	0.6106355327142126
1104	30-31	0.6241947304081634
1104	32-33	0.6663694872236547
1104	34-35	0.6777730712508614
1104	36-37	0.6399157750464894
1104	38-39	0.6501643551062699
1104	40-41	0.6697172823002333
1104	42-43	0.6722897816271356
1104	44-45	0.6255267190877802
1104	46-47	0.5871819902848614
1104	48-49	0.6207456458066432
1104	50-51	0.5076667469408136
1104	52-53	0.544803299816877
1104	54-55	0.6646322040471375
1104	56-57	0.664395702773767
1104	58-59	0.6164957016155341
1104	60-61	0.583738337952667
1104	62-63	0.6098361828503229
1104	64-65	0.5263421467469129
1104	66-67	0.4694893111855549
1104	68-69	0.5072210104038746
1104	70-71	0.5234417633619373
1104	72-73	0.5108404177731742
1104	74-75	0.4537440666248571
1104	76-77	0.3617952705814531
1104	78-79	0.4282553978133379
1104	80-81	0.4632646762873165
1104	82-83	0.4772859298178034
1104	84-85	0.4968930757200738
1104	86-87	0.4801230446555209
1104	88-89	0.42010219714448027
1104	90-91	0.5696141918542921
1104	92-93	0.5769374197976234
1104	94-95	0.6286975553635799
1104	96-97	0.5796503659431522
1104	98-99	0.5413760917776465
1104	100	0.610793094812415
1105	1	0.24990475177651206
1105	2	0.3315752382378463
1105	3	0.31006833005146106
1105	4	0.4532531026238047
1105	5	0.28675059975076067
1105	6	0.3372541315765716
1105	7	0.3641738749261023
1105	8	0.3671324824630773
1105	9	0.5272046610143875
1105	10-11	0.5333671035253715
1105	12-13	0.4384669147662734
1105	14-15	0.5869419725408136
1105	16-17	0.5656398166128085
1105	18-19	0.52546420821146
1105	20-21	0.4530232394004301
1105	22-23	0.5409737094683535
1105	24-25	0.5525016593596987
1105	26-27	0.5106399486853235
1105	28-29	0.5092017769273198
1105	30-31	0.520089079908935
1105	32-33	0.5448930374976086
1105	34-35	0.5631733372028478
1105	36-37	0.5421865489018671
1105	38-39	0.5529965282661848
1105	40-41	0.49882497292567507
1105	42-43	0.4977604802091449
1105	44-45	0.45383696679349583
1105	46-47	0.524081391868414
1105	48-49	0.41128008500015767
1105	50-51	0.476136029325005
1105	52-53	0.5026212870471909
1105	54-55	0.4500378414976467
1105	56-57	0.4431601571984487
1105	58-59	0.4459821914664346
1105	60-61	0.45270706111979564
1105	62-63	0.4355407694223885
1105	64-65	0.46232895776999783
1105	66-67	0.40004463875935414
1105	68-69	0.368123197970597
1105	70-71	0.45983348594871387
1105	72-73	0.39283977787717816
1105	74-75	0.3795968047893439
1105	76-77	0.378888744640804
1105	78-79	0.38915185727194057
1105	80-81	0.3300426402756216
1105	82-83	0.3818802280711182
1105	84-85	0.2652182314099676
1105	86-87	0.2736322039759713
1105	88-89	0.3274231270213477
1105	90-91	0.3609866529456909
1105	92-93	0.34651156598962274
1105	94-95	0.3922766844667507
1105	96-97	0.3407560083308354
1105	98-99	0.34468042242538033
1105	100	0.409380720697996
1106	1	0.09631273335563506
1106	2	0.19310964562181354
1106	3	0.17508255337681433
1106	4	0.2654257014944932
1106	5	0.2284338396508474
1106	6	0.23988212461243563
1106	7	0.2630788027508899
1106	8	0.20373789385840269
1106	9	0.3904966194487898
1106	10-11	0.4099591287031714
1106	12-13	0.30595169479460793
1106	14-15	0.4487073541155837
1106	16-17	0.36142120609167705
1106	18-19	0.3997823782562193
1106	20-21	0.3444898823427778
1106	22-23	0.34943240251404717
1106	24-25	0.41362351898533944
1106	26-27	0.41372703717925674
1106	28-29	0.40997597028196253
1106	30-31	0.4319858085816435
1106	32-33	0.4353353682760783
1106	34-35	0.41860139960979126
1106	36-37	0.41220573856647036
1106	38-39	0.43984226093072465
1106	40-41	0.4547431751033528
1106	42-43	0.39540073706236
1106	44-45	0.4025932798252754
1106	46-47	0.4103463669015781
1106	48-49	0.3244941135759447
1106	50-51	0.35455816741100676
1106	52-53	0.3290440512748347
1106	54-55	0.438582415049396
1106	56-57	0.35486652830223875
1106	58-59	0.37047743046259285
1106	60-61	0.4185983332008618
1106	62-63	0.46247300573154604
1106	64-65	0.41199182479297747
1106	66-67	0.38569533430962366
1106	68-69	0.34023500704694953
1106	70-71	0.3705172716581231
1106	72-73	0.3076966399465775
1106	74-75	0.30949968526610405
1106	76-77	0.2997579178260281
1106	78-79	0.31608856065873425
1106	80-81	0.319377023184515
1106	82-83	0.2815830735620395
1106	84-85	0.3279328857519985
1106	86-87	0.3212988879103378
1106	88-89	0.35400362788961104
1106	90-91	0.2733700303116393
1106	92-93	0.3201146657661198
1106	94-95	0.2965531042279004
1106	96-97	0.2777317367084926
1106	98-99	0.3554182635280476
1106	100	0.3075495191742377
1107	1	0.09397595576407625
1107	2	0.13530383917484912
1107	3	0.11176259364169283
1107	4	0.21443578015771436
1107	5	0.07816996978210966
1107	6	0.24087276636831945
1107	7	0.16031386698934824
1107	8	0.1743915563053804
1107	9	0.2540503551820379
1107	10-11	0.31406550120307486
1107	12-13	0.23115929010769065
1107	14-15	0.3475900823296172
1107	16-17	0.2062694727305754
1107	18-19	0.19389247728764758
1107	20-21	0.16956333234471543
1107	22-23	0.37278619574983907
1107	24-25	0.38788332110586765
1107	26-27	0.24890217106259627
1107	28-29	0.2763964337210183
1107	30-31	0.42389995558250604
1107	32-33	0.36888433614472405
1107	34-35	0.28346721500304994
1107	36-37	0.35888305106266216
1107	38-39	0.38663112377537345
1107	40-41	0.2556927693436748
1107	42-43	0.27150549975303306
1107	44-45	0.3329923134157937
1107	46-47	0.30759362914526633
1107	48-49	0.26305945788060825
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2209	7	0.023576499988649857
2209	8	0.10753536639413852
2209	9	0.13539113777083145
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2209	26-27	0.08919143651078798
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2210	3	0.17911800428224822
2210	4	0.2026978996013682
2210	5	0.1492140225498062
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2210	7	0.13491803895609422
2210	8	0.10058198075064695
2210	9	0.1403898508829542
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2210	26-27	0.20350141475459083
2210	28-29	0.1504277603324624
2210	30-31	0.09410119453330879
2210	32-33	0.05002990327558621
2210	34-35	0.14969638808840813
2210	36-37	0.10493451861466951
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2211	2	-0.1969958837694321
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2211	14-15	-0.14889068276816886
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2211	30-31	-0.07223655635500847
2211	32-33	-0.21577562390634597
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2211	36-37	-0.26387948573304243
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2211	88-89	-0.0029483487052210933
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2212	60-61	-0.19915381651387065
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2212	96-97	-0.06028445327386578
2212	98-99	-0.16468440522162098
2212	100	-0.18087531928312828
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2213	4	-0.29676068297013103
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2213	24-25	-0.6237716904381969
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2213	30-31	-0.6472728888829735
2213	32-33	-0.6854989063197721
2213	34-35	-0.7073169502252838
2213	36-37	-0.7763451979439537
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2213	42-43	-1.0437817965440956
2213	44-45	-0.8101877835490399
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2213	60-61	-0.701758424793347
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2213	64-65	-0.6573353392710217
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2213	68-69	-0.709740595747121
2213	70-71	-0.7781119396856795
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2213	80-81	-0.5038151120159711
2213	82-83	-0.464169544023342
2213	84-85	-0.5487186777307969
2213	86-87	-0.595152215711007
2213	88-89	-0.5743092778582088
2213	90-91	-0.662132879284016
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2213	94-95	-0.5590667941491816
2213	96-97	-0.508390336562087
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2213	100	-0.4287971914694886
2214	1	-0.6920295242007732
2214	2	-0.7765141653198491
2214	3	-0.6115051620253773
2214	4	-0.5974296476152432
2214	5	-0.5655453680469691
2214	6	-0.569243928018885
2214	7	-0.5979472207650076
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2214	9	-1.1814161318860528
2214	10-11	-0.759498117624176
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2214	14-15	-3.30853772025074
2214	16-17	-1.5693606864239484
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2214	22-23	-0.6983953825673126
2214	24-25	-0.6420058637841919
2214	26-27	-0.8097721419504964
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2214	32-33	-0.9463982490232965
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2214	36-37	-0.8014302698575477
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2214	52-53	-0.6300911844199462
2214	54-55	-0.8333032816945263
2214	56-57	-0.7487365793651364
2214	58-59	-0.6867041703052905
2214	60-61	-0.8075176398230326
2214	62-63	-0.8702053460124333
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2214	80-81	-0.6555309565843928
2214	82-83	-0.6728747844199923
2214	84-85	-0.7010477084729878
2214	86-87	-0.6690502234757716
2214	88-89	-0.7095581246675486
2214	90-91	-0.6954231502827817
2214	92-93	-0.6683627163650527
2214	94-95	-0.6786385613094339
2214	96-97	-0.6022207289852659
2214	98-99	-0.6313421165485629
2214	100	-0.38205343945383774
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2215	2	-1.0628503099479083
2215	3	-0.8873621715363491
2215	4	-2.4168871956473836
2215	5	-1.082774884290295
2215	6	-0.8230339783373353
2215	7	-0.864962472677334
2215	8	-0.7384348006901433
2215	9	-1.1266990371913508
2215	10-11	-0.8159625992232975
2215	12-13	-1.026196438654317
2215	14-15	-1.0500697200612876
2215	16-17	-1.0175505350500842
2215	18-19	-0.9058256413926671
2215	20-21	-1.1186268743924686
2215	22-23	-0.9623806091229739
2215	24-25	-0.9795762544347966
2215	26-27	-1.0019910015091753
2215	28-29	-0.9502378511454239
2215	30-31	-3.6515155957706256
2215	32-33	-1.506473240132884
2215	34-35	-1.309006840340622
2215	36-37	-1.0389044719055889
2215	38-39	-0.9104784796187957
2215	40-41	-0.9670420129531649
2215	42-43	-0.9790448724203671
2215	44-45	-0.8864022095987139
2215	46-47	-1.0564858062049822
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2215	50-51	-1.0783359362153675
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2215	54-55	-0.9625392449367567
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2215	58-59	-1.0254041639039642
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2215	62-63	-0.9596039557439937
2215	64-65	-0.9748782592383307
2215	66-67	-0.9019387420228995
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2215	70-71	-1.07344645202096
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2215	80-81	-0.8959229470775831
2215	82-83	-0.8195936618101243
2215	84-85	-0.8499126548374321
2215	86-87	-0.8509233856968947
2215	88-89	-0.8295064652004029
2215	90-91	-0.8825833106061189
2215	92-93	-0.9330948558572061
2215	94-95	-0.9132487449036617
2215	96-97	-1.1574636772075593
2215	98-99	-0.9794021893121432
2215	100	-0.6248549600662656
2216	1	-0.5976342195074125
2216	2	-0.7816434142134234
2216	3	-0.8183364336511936
2216	4	-0.6801362852578592
2216	5	-0.5613542550144857
2216	6	-0.4933249936402646
2216	7	-0.614156637303104
2216	8	-0.654840722282259
2216	9	-0.8051017560484439
2216	10-11	-1.2701354600979187
2216	12-13	-0.8522113511553826
2216	14-15	-0.9401580372632736
2216	16-17	-0.9570369299125332
2216	18-19	-1.2048492350569475
2216	20-21	-1.3394073059259526
2216	22-23	-1.2004855095470575
2216	24-25	-1.205705369461576
2216	26-27	-1.6169039017356681
2216	28-29	-1.4619453968819371
2216	30-31	-1.6249496534323313
2216	32-33	-1.8823026195262642
2216	34-35	-1.4622363251114265
2216	36-37	-1.6102466044860506
2216	38-39	-1.4546170042700979
2216	40-41	-1.2897119284242038
2216	42-43	-1.3344810336388306
2216	44-45	-1.2377645070858492
2216	46-47	-1.3643820336485675
2216	48-49	-1.3183926911047266
2216	50-51	-1.4613632367996985
2216	52-53	-1.3743898653751927
2216	54-55	-1.446232370382532
2216	56-57	-1.3247780818617017
2216	58-59	-1.3111440598088393
2216	60-61	-1.3617885963909444
2216	62-63	-1.3870106908677258
2216	64-65	-1.296075441739589
2216	66-67	-1.2845980386735505
2216	68-69	-1.3618433191780284
2216	70-71	-1.1990878409970946
2216	72-73	-1.1770910793760336
2216	74-75	-1.3242075832455384
2216	76-77	-1.323103902191292
2216	78-79	-1.153000650748659
2216	80-81	-1.2476239665991002
2216	82-83	-1.2801671092956717
2216	84-85	-1.2434184784115914
2216	86-87	-1.2426285751604524
2216	88-89	-1.2501824548412799
2216	90-91	-1.4114056244237574
2216	92-93	-1.430694563374999
2216	94-95	-1.368365044250364
2216	96-97	-1.3600077971402271
2216	98-99	-1.4031739648792012
2216	100	-1.0008024898963832
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	19.0
3	42.0
4	45.0
5	54.0
6	50.0
7	212.0
8	655.0
9	1159.0
10	2013.0
11	4376.0
12	8307.0
13	11941.0
14	15934.0
15	19804.0
16	23266.0
17	26489.0
18	30093.0
19	33353.0
20	38021.0
21	42475.0
22	48084.0
23	54651.0
24	62698.0
25	72578.0
26	79886.0
27	87926.0
28	100368.0
29	119828.0
30	139271.0
31	160092.0
32	197089.0
33	252837.0
34	361089.0
35	504893.0
36	797552.0
37	1297826.0
38	1124157.0
39	427079.0
40	807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.61768797660393	21.134394069142232	12.22114666700957	24.026771287244266
2	15.424044825364597	19.79987049582528	41.032942217710186	23.74314246109994
3	18.333610184252557	27.054674450163503	29.031373744022826	25.580341621561114
4	11.786633580648715	16.689257115096424	40.95873635360389	30.565372950650975
5	12.665744993486033	38.958712121705105	35.63528748003837	12.74025540477049
6	29.402992213295576	39.16018967646718	18.344616007319477	13.092202102917769
7	26.46558528730606	31.997747180559376	24.321079479235603	17.21558805289896
8	25.98091991877577	35.36289799109605	20.032637409153896	18.623544680974288
9	26.12333635758313	14.960096658862835	21.635278077247378	37.281288906306656
10-11	25.429889612549893	26.025937919158743	28.047546886807112	20.496625581484256
12-13	25.5561981454368	24.038465918334147	27.91759394826745	22.487741987961606
14-15	22.9971626044888	25.27809249715777	25.260327764203428	26.464417134150008
16-17	21.85289033136649	27.7190339882858	26.610783448244675	23.81729223210304
18-19	21.171659953277413	28.654301666525672	28.18398482492045	21.990053555276464
20-21	22.99618144423481	27.746710466112706	27.103896521165144	22.15321156848734
22-23	23.461755861925017	27.40882835032188	26.74910123914591	22.38031454860719
24-25	22.832520353242884	27.436857931729193	27.73998475987696	21.99063695515096
26-27	22.811779552471883	27.720270769102623	26.56487039489086	22.903079283534634
28-29	21.854564128143625	28.259703695135478	27.125786198822187	22.759945977898706
30-31	22.743251771238718	27.889674551510428	27.102169022108985	22.26490465514187
32-33	23.263097604769328	28.01021798924789	26.134452475880813	22.592231930101963
34-35	23.135783611582518	27.765616523788765	27.227978922686336	21.870620941942377
36-37	22.689006997812736	27.367814031157934	26.952137902400263	22.991041068629066
38-39	22.901897851473706	27.520786462973852	27.55362571082899	22.02368997472346
40-41	22.507702609792897	26.563420729903825	28.20209422482412	22.726782435479155
42-43	22.47089795877525	26.85686565416921	27.462519053782085	23.209717333273456
44-45	22.61354643647206	27.95820986399315	27.24889930149364	22.179344398041152
46-47	22.556746556522015	27.636639801687558	26.77804521175442	23.028568430036003
48-49	23.365275384151143	27.16123026971633	27.010465351934098	22.463028994198428
50-51	22.884666341299038	26.974324296069792	27.050570639446647	23.090438723184526
52-53	22.15772737302317	27.701038101021698	26.90189427889112	23.239340247064007
54-55	22.29393378285149	27.560488069979044	26.832003436470846	23.31357471069862
56-57	23.492163258817833	26.888188840320453	26.5834906354561	23.036157265405606
58-59	22.3492495834635	27.377373932625222	27.55737139435593	22.716005089555345
60-61	22.352299535161308	28.060700588659003	26.728078395015427	22.858921481164263
62-63	22.80970779562818	27.259183272531146	26.324026781977278	23.60708214986339
64-65	21.862922368567588	27.533906344225812	26.916925948441712	23.686245338764888
66-67	22.705557003615596	27.038787183009084	26.741650144138916	23.514005669236404
68-69	22.064284154790602	27.185261697456742	27.049741339956313	23.700712807796343
70-71	22.097570769052556	26.748059699938437	27.278860133097492	23.875509397911518
72-73	22.70605431222059	26.834770962547232	27.79303768574507	22.666137039487104
74-75	22.897787684410474	27.10370102216383	27.220560420742647	22.777950872683046
76-77	22.49930426238838	27.072779482537168	26.943149797168388	23.484766457906066
78-79	22.34082761100477	26.989202350378154	27.020099263859475	23.649870774757595
80-81	21.848412322076328	27.317861225128638	27.75166336511398	23.082063087681057
82-83	22.732248500614283	26.257798924787775	28.776012041127448	22.23394053347049
84-85	23.008705360338727	26.88650849743131	27.355479564383806	22.749306577846156
86-87	21.58268968593326	27.623770696307353	27.803509573423803	22.99003004433559
88-89	22.86883453596976	26.25107052863941	27.50659888170431	23.373496053686523
90-91	22.426126669421222	26.223786662918	27.870874906923888	23.47921176073689
92-93	22.71588664728261	26.567802721582606	27.654802304513304	23.061508326621478
94-95	21.451520430654735	26.63275386444554	28.304602843742536	23.611122861157188
96-97	21.819648601493032	26.472899961798852	27.90639482026262	23.801056616445496
98-99	21.13075257008633	27.252070813377667	27.782093285744793	23.83508333079121
100	22.060485684769727	26.50910035095319	26.864669200766816	24.565744763510263
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	587.0
1	393.0
2	261.5
3	303.5
4	280.5
5	264.5
6	247.0
7	261.5
8	301.0
9	317.0
10	304.5
11	291.5
12	288.5
13	308.0
14	344.0
15	590.0
16	771.5
17	905.5
18	1291.5
19	1744.5
20	2353.5
21	3065.5
22	4308.0
23	6173.5
24	8399.0
25	10988.5
26	15044.5
27	20223.5
28	27357.5
29	40772.0
30	58941.0
31	76189.5
32	99620.0
33	121173.0
34	135152.0
35	154045.0
36	171273.0
37	201436.5
38	213339.5
39	194828.0
40	179641.5
41	163536.0
42	159035.0
43	169541.0
44	185756.5
45	221166.5
46	254299.0
47	277142.0
48	293721.0
49	287854.0
50	270591.5
51	272909.5
52	276170.5
53	262019.5
54	245594.0
55	210601.5
56	182617.5
57	148635.0
58	113201.0
59	108823.0
60	90916.0
61	61007.0
62	44226.0
63	37271.5
64	27468.0
65	12312.0
66	5924.0
67	3808.5
68	2540.0
69	1589.0
70	974.0
71	527.0
72	279.5
73	183.0
74	117.5
75	75.0
76	60.0
77	49.0
78	40.5
79	41.5
80	30.0
81	20.5
82	20.0
83	23.0
84	23.0
85	19.5
86	18.0
87	13.0
88	11.5
89	11.5
90	10.5
91	9.0
92	11.0
93	11.0
94	11.0
95	11.0
96	8.5
97	7.0
98	5.5
99	8.5
100	16.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.055685519162130596
2	0.008134022664640752
3	0.00831297116326285
4	0.010037383968166688
5	0.0037091143350761833
6	0.002147381983465159
7	9.110105384397642E-4
8	0.0013990518983182094
9	6.669898585005417E-4
10-11	5.693815865248527E-4
12-13	3.253609065856301E-4
14-15	1.545464306281743E-4
16-17	5.61247563860212E-4
18-19	0.0016268045329281503
20-21	0.001016752833080094
22-23	0.0013746498303242871
24-25	0.0014315879889767724
26-27	0.0037904545617225907
28-29	0.0044981145335463366
30-31	0.005417259094650742
32-33	0.006661764562340777
34-35	0.0036033720404358535
36-37	0.008589527933860636
38-39	0.00669430065299934
40-41	0.006539754222371165
42-43	0.01513741617889644
44-45	0.020554675273547183
46-47	0.018911602695289752
48-49	0.022116407625158208
50-51	0.018732654196667652
52-53	0.025508295076313404
54-55	0.019196293488552174
56-57	0.015267560541530693
58-59	0.017894849862209655
60-61	0.01654460209987929
62-63	0.011753662750405888
64-65	0.011623518387771635
66-67	0.014088127255157783
68-69	0.005563671502614275
70-71	0.008914888840446264
72-73	0.00907756929373908
74-75	0.01211969377031472
76-77	0.010826384166636841
78-79	0.012607735130193165
80-81	0.010891456347953967
82-83	0.006824445015633592
84-85	0.011257487367862801
86-87	0.009817765356221388
88-89	0.008524455752543509
90-91	0.013673292099261106
92-93	0.009101971361733003
94-95	0.007678517395420871
96-97	0.009890971560203155
98-99	0.009476136404306477
100	0.25570113648564674
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6147020.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.80006343138326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.38269100736137	47.55203032042616
2	15.233084525428339	19.742096870067588
3	5.583765778719728	10.85485129941077
4	2.5250960981958794	6.545055493337257
5	1.2774790198349166	4.139036075878197
6	0.7004875263774137	2.7234981685289505
7	0.4060444870845092	1.8418195963327777
8	0.2664825840548069	1.381447068008833
9	0.17073116955770262	0.9957051555348045
>10	0.4516380867650314	4.041651170942324
>50	0.001913440202446991	0.08442193579714763
>100	5.483158976591397E-4	0.07408358588618413
>500	3.7960520160106816E-5	0.024303259848920667
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.253609065856301E-5	0.0
3	0.0	0.0	0.0	4.8804135987844516E-5	0.0
4	0.0	0.0	0.0	6.507218131712602E-5	0.0
5	0.0	0.0	0.0	6.507218131712602E-5	0.0
6	0.0	0.0	0.0	6.507218131712602E-5	0.0
7	0.0	0.0	0.0	8.134022664640752E-5	0.0
8	0.0	0.0	0.0	8.134022664640752E-5	0.0
9	0.0	0.0	0.0	2.1148458928065957E-4	0.0
10-11	0.0	0.0	0.0	2.2775263460994108E-4	0.0
12-13	0.0	0.0	0.0	2.4402067993922259E-4	0.0
14-15	0.0	0.0	0.0	3.1722688392098934E-4	0.0
16-17	0.0	0.0	0.0	4.0670113323203763E-4	0.0
18-19	0.0	0.0	0.0	4.2296917856131914E-4	0.0
20-21	0.0	0.0	0.0	5.693815865248527E-4	0.0
22-23	0.0	0.0	0.0	6.91391926494464E-4	0.0
24-25	0.0	0.0	0.0	7.5646410781159E-4	0.0
26-27	0.0	0.0	0.0	0.0011062270823911423	0.0
28-29	0.0	0.0	0.0	0.0032861451565148643	0.0
30-31	0.0	0.0	0.0	0.011054136801246783	0.0
32-33	0.0	0.0	0.0	0.02519106819239241	0.0
34-35	0.0	0.0	0.0	0.04708785720560532	0.0
36-37	0.0	0.0	0.0	0.08386177367244615	0.0
38-39	0.0	0.0	0.0	0.14066978796229718	0.0
40-41	0.0	0.0	0.0	0.20271611284817684	0.0
42-43	0.0	0.0	0.0	0.26860983045443154	0.0
44-45	0.0	0.0	0.0	0.3496165621715888	0.0
46-47	0.0	0.0	0.0	0.4517880208621413	0.0
48-49	0.0	0.0	0.0	0.5697313494994323	0.0
50-51	0.0	0.0	0.0	0.6975656496969262	0.0
52-53	0.0	0.0	0.0	0.8227401244830828	0.0
54-55	0.0	0.0	0.0	0.9578462409427657	0.0
56-57	0.0	0.0	0.0	1.1270013762766349	0.0
58-59	0.0	0.0	0.0	1.3316208504283376	0.0
60-61	0.0	0.0	0.0	1.544081522428754	0.0
62-63	0.0	0.0	0.0	1.7674336507771247	0.0
64-65	0.0	0.0	0.0	2.0020514005160224	0.0
66-67	0.0	8.134022664640752E-6	0.0	2.269213374936148	0.0
68-69	0.0	1.6268045329281504E-5	0.0	2.5690497184001355	0.0
70-71	0.0	1.6268045329281504E-5	0.0	2.908848515215503	0.0
72-73	0.0	1.6268045329281504E-5	0.0	3.2594167580388547	0.0
74-75	0.0	1.6268045329281504E-5	0.0	3.6028758650533104	0.0
76-77	0.0	1.6268045329281504E-5	0.0	4.006347791287486	0.0
78-79	0.0	1.6268045329281504E-5	0.0	4.43915913727302	0.0
80-81	0.0	1.6268045329281504E-5	0.0	4.9084109047961455	0.0
82-83	0.0	1.6268045329281504E-5	0.0	5.4081896593796674	0.0
84-85	0.0	1.6268045329281504E-5	0.0	5.891171657160705	0.0
86-87	0.0	1.6268045329281504E-5	0.0	6.40697443639357	0.0
88	0.0	1.6268045329281504E-5	0.0	6.823224912233895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6340	0.0	42.650238	94
GTACTAG	6570	0.0	35.20665	1
CAACGCA	10410	0.0	33.677784	5
GCATCGT	6775	0.0	32.253834	8
GTATCAA	12245	0.0	31.867228	1
CTGTGCG	9020	0.0	31.728426	9
TAGGCAT	8235	0.0	31.216131	5
TAGTACT	9565	0.0	31.10225	4
ATCAACG	11435	0.0	30.86575	3
CTAGGCA	9220	0.0	28.595982	4
ACTGTGC	10345	0.0	28.573135	8
GTACTGT	10470	0.0	27.963154	6
CTAGTAC	10935	0.0	27.678347	3
AACGCAG	13660	0.0	27.453453	6
TATCAAC	14130	0.0	27.373526	2
TACTGTG	11495	0.0	27.186317	7
GGTATCA	7870	0.0	26.882076	1
ACGCAGA	12960	0.0	26.832703	7
AGGGCTA	7990	0.0	26.585638	5
CGCAGAG	13055	0.0	26.38547	8
>>END_MODULE