FastQCFastQC Report
Mon 16 Jan 2017
ERR1146654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6147020
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150790.24530585552023582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123480.20087782372596805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83880.13645636422201327No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114550.043.40131
GTATCAA208150.040.8775561
GGGCACC60600.039.8857694
GTGGTAT26500.036.918181
GTACTAG63000.034.193831
CAACGCA245500.033.1382145
ATCAACG247700.032.9249153
TATCAAC273100.031.1386342
CTGTGCG84550.030.9121729
TCCGCAC3600.030.0487863
AACGCAG285700.029.6426816
TAGGCAT85200.029.4016115
TAGTACT91450.029.2123344
TATTCTC39800.029.167385
ACTGTGC96450.028.2637968
CTAACGC31200.027.5865963
ACGCAGA297950.027.2409277
TCAACGC302900.027.1265934
CGCAGAG299150.027.1022178
TACTGTG109350.026.9906777
Produced by FastQC (version 0.11.5)