##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6147020
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.084604409941726	33.0	31.0	34.0	30.0	34.0
2	32.14649456159245	33.0	31.0	34.0	30.0	34.0
3	32.30502975425491	34.0	31.0	34.0	30.0	34.0
4	35.92705278329988	37.0	35.0	37.0	35.0	37.0
5	35.83559985163543	37.0	35.0	37.0	35.0	37.0
6	35.869221671639266	37.0	35.0	37.0	35.0	37.0
7	35.78819802115497	37.0	35.0	37.0	33.0	37.0
8	35.79316351663082	37.0	35.0	37.0	33.0	37.0
9	37.489589752432884	39.0	37.0	39.0	34.0	39.0
10-11	37.425325442246816	39.0	37.0	39.0	34.0	39.0
12-13	37.39874418824081	39.0	37.0	39.0	34.0	39.0
14-15	38.598159921392806	40.0	38.0	41.0	34.0	41.0
16-17	38.600766550295916	40.0	38.0	41.0	34.0	41.0
18-19	38.66287867942515	40.0	38.0	41.0	34.0	41.0
20-21	38.66112791238682	40.0	38.0	41.0	34.0	41.0
22-23	38.62597120230616	40.0	38.0	41.0	34.0	41.0
24-25	38.55022978614028	40.0	38.0	41.0	34.0	41.0
26-27	38.403411246425094	40.0	38.0	41.0	34.0	41.0
28-29	38.34392063146045	40.0	38.0	41.0	34.0	41.0
30-31	38.209230082218696	40.0	37.5	41.0	33.5	41.0
32-33	38.06665319130245	40.0	37.0	41.0	33.0	41.0
34-35	37.96261367296674	40.0	37.0	41.0	33.0	41.0
36-37	37.95637243737616	40.0	37.0	41.0	33.0	41.0
38-39	37.86335224222469	40.0	37.0	41.0	33.0	41.0
40-41	37.7492057126868	40.0	37.0	41.0	33.0	41.0
42-43	37.62056630692596	40.0	36.5	41.0	32.5	41.0
44-45	37.411091227944596	40.0	36.0	41.0	32.0	41.0
46-47	37.16484532342501	40.0	36.0	41.0	31.0	41.0
48-49	36.89745852461843	39.0	35.0	41.0	31.0	41.0
50-51	36.69296813740642	39.0	35.0	41.0	30.0	41.0
52-53	36.51251964366473	39.0	35.0	41.0	30.0	41.0
54-55	36.33282671928837	39.0	35.0	41.0	30.0	41.0
56-57	36.106834124502605	38.0	35.0	40.5	30.0	41.0
58-59	35.83048891983432	38.0	35.0	40.0	29.0	41.0
60-61	35.653520649029936	37.0	35.0	40.0	29.0	41.0
62-63	35.444035971901826	37.0	34.5	40.0	29.0	41.0
64-65	35.203479816236154	36.0	34.0	40.0	29.0	41.0
66-67	34.96100126565393	36.0	34.0	39.0	29.0	41.0
68-69	34.7156206584654	35.0	34.0	39.0	29.0	41.0
70-71	34.502938090326694	35.0	34.0	39.0	29.0	41.0
72-73	34.27066480994043	35.0	34.0	38.0	28.5	40.5
74-75	34.00907049269402	35.0	34.0	37.0	28.0	40.0
76-77	33.76683474268833	35.0	34.0	37.0	28.0	39.0
78-79	33.53907527549934	35.0	33.0	36.0	28.0	39.0
80-81	33.40402056606291	35.0	33.0	36.0	28.0	39.0
82-83	33.24077959726827	35.0	33.0	36.0	28.0	37.0
84-85	33.10478060914069	35.0	33.0	35.0	28.0	37.0
86-87	32.95286106113206	35.0	33.0	35.0	27.5	37.0
88-89	32.83985695507742	35.0	33.0	35.0	27.5	36.0
90-91	32.76924347082001	35.0	33.0	35.0	27.5	36.0
92-93	32.69846885157361	35.0	33.0	35.0	27.0	36.0
94-95	32.64836302793874	35.0	33.0	35.0	27.0	36.0
96-97	32.55544198652355	35.0	33.0	35.0	27.0	35.0
98-99	32.47444273810724	35.0	33.0	35.0	27.0	35.0
100	31.235938226978277	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3727600721765114
1101	2	0.4777966612792781
1101	3	0.3969221510932641
1101	4	0.30329644281287926
1101	5	0.2505324054710485
1101	6	0.2817394128041286
1101	7	0.33452487964871125
1101	8	0.3139352887320257
1101	9	0.4200927038937792
1101	10-11	0.41164759135904916
1101	12-13	0.4177955947048275
1101	14-15	0.5548459619276755
1101	16-17	0.6039582607915079
1101	18-19	0.5558348692113242
1101	20-21	0.5484060143688154
1101	22-23	0.570012264568156
1101	24-25	0.5705466001269173
1101	26-27	0.6131080751505849
1101	28-29	0.6082101901419819
1101	30-31	0.6119952291737434
1101	32-33	0.6704668611778004
1101	34-35	0.7119090506557697
1101	36-37	0.6223444133644236
1101	38-39	0.6705845840454003
1101	40-41	0.6478816054745877
1101	42-43	0.5892888600500967
1101	44-45	0.5421099186130292
1101	46-47	0.6098990527842574
1101	48-49	0.5943785354348066
1101	50-51	0.5884012791426869
1101	52-53	0.627896412551948
1101	54-55	0.625376437466592
1101	56-57	0.6003586792888385
1101	58-59	0.5472087599822117
1101	60-61	0.5523425333288614
1101	62-63	0.5854362137860107
1101	64-65	0.5608681405239295
1101	66-67	0.5248413051551211
1101	68-69	0.5339574663265552
1101	70-71	0.5079943723451379
1101	72-73	0.5731220818602694
1101	74-75	0.5139837402083316
1101	76-77	0.500880545449661
1101	78-79	0.48285192236949825
1101	80-81	0.469219834477137
1101	82-83	0.4746817429390475
1101	84-85	0.4715530552264582
1101	86-87	0.3783478583973974
1101	88-89	0.4369596757506784
1101	90-91	0.4317577782777704
1101	92-93	0.4687945076340725
1101	94-95	0.4549564292726487
1101	96-97	0.4927969306729807
1101	98-99	0.489703958822588
1101	100	0.8436641635691693
1102	1	0.37945705689148923
1102	2	0.3935141340978703
1102	3	0.339489763975763
1102	4	0.2200795716779993
1102	5	0.24665744420780555
1102	6	0.23012061430760156
1102	7	0.30955985305752165
1102	8	0.2920041171789478
1102	9	0.3494578506062638
1102	10-11	0.3081971724252526
1102	12-13	0.3752180713288169
1102	14-15	0.5155400655106774
1102	16-17	0.46445741398640195
1102	18-19	0.4939489280108518
1102	20-21	0.5095472195112407
1102	22-23	0.5306599431184438
1102	24-25	0.5391306831932567
1102	26-27	0.594056854092301
1102	28-29	0.6191926466920066
1102	30-31	0.547020164211709
1102	32-33	0.6213105006251425
1102	34-35	0.6414286752746321
1102	36-37	0.5917650157514629
1102	38-39	0.6123169285811372
1102	40-41	0.5596417438712251
1102	42-43	0.6059019155337779
1102	44-45	0.5818755827005333
1102	46-47	0.642243366124319
1102	48-49	0.5830799994704492
1102	50-51	0.6167586169283794
1102	52-53	0.585283931404291
1102	54-55	0.5499590413257494
1102	56-57	0.5428095693533237
1102	58-59	0.46846292181611915
1102	60-61	0.48446778028327486
1102	62-63	0.5436133751857639
1102	64-65	0.5261752973666702
1102	66-67	0.5520040670803894
1102	68-69	0.5328706827202367
1102	70-71	0.4827636701644309
1102	72-73	0.45209144821904346
1102	74-75	0.5095651960209153
1102	76-77	0.42560152092577397
1102	78-79	0.48589333537513824
1102	80-81	0.4447793308322545
1102	82-83	0.404274480034573
1102	84-85	0.3769532595105076
1102	86-87	0.43195897188304144
1102	88-89	0.4260745171733973
1102	90-91	0.413654013603761
1102	92-93	0.38554486793194087
1102	94-95	0.3734969853958816
1102	96-97	0.4196169535394034
1102	98-99	0.43216298021764743
1102	100	0.633654134233133
1103	1	0.29462552382426566
1103	2	0.3472904957427261
1103	3	0.26983485583030387
1103	4	0.2334719654616748
1103	5	0.21739397055750942
1103	6	0.22796635213303063
1103	7	0.2568180974770229
1103	8	0.2290224797015128
1103	9	0.2728539549591247
1103	10-11	0.3364613704817785
1103	12-13	0.29459486073953656
1103	14-15	0.38686462719941517
1103	16-17	0.363097790786469
1103	18-19	0.3673337944997144
1103	20-21	0.355935676897964
1103	22-23	0.3557441847249905
1103	24-25	0.36939994232519524
1103	26-27	0.3673034428909858
1103	28-29	0.4327474286138866
1103	30-31	0.4204060900348523
1103	32-33	0.42527861961456637
1103	34-35	0.4142652682575729
1103	36-37	0.4125927295952536
1103	38-39	0.4052273707668803
1103	40-41	0.42992944032588554
1103	42-43	0.40186453392441734
1103	44-45	0.40987272593247326
1103	46-47	0.4698541088175574
1103	48-49	0.45700203764207714
1103	50-51	0.4773214017526968
1103	52-53	0.4120488570115981
1103	54-55	0.3560837753269155
1103	56-57	0.38489774289644885
1103	58-59	0.42810724821578816
1103	60-61	0.4770846106917972
1103	62-63	0.411279564995894
1103	64-65	0.41120293885458636
1103	66-67	0.3660092779345234
1103	68-69	0.3854146416357267
1103	70-71	0.41104096382272814
1103	72-73	0.4133127107661849
1103	74-75	0.3200243690327653
1103	76-77	0.3345125003166629
1103	78-79	0.3601119536901294
1103	80-81	0.3201465797224188
1103	82-83	0.3135169641471194
1103	84-85	0.3363817939436231
1103	86-87	0.36877852524006016
1103	88-89	0.40778928140729676
1103	90-91	0.31880306282783977
1103	92-93	0.3211414365988077
1103	94-95	0.32838815697543566
1103	96-97	0.3196656985271318
1103	98-99	0.3882003403280976
1103	100	0.6526490459049512
1104	1	0.2828696555417025
1104	2	0.30790959530371254
1104	3	0.25104054443310275
1104	4	0.2036939117041925
1104	5	0.17675703349020466
1104	6	0.21969111002973563
1104	7	0.2661334604529628
1104	8	0.22859265711512933
1104	9	0.2552865071067174
1104	10-11	0.2761330224047924
1104	12-13	0.2799204066938188
1104	14-15	0.3452545024886362
1104	16-17	0.34913730756229455
1104	18-19	0.3529287711305713
1104	20-21	0.420650103090388
1104	22-23	0.40722413568713023
1104	24-25	0.4125076768848146
1104	26-27	0.4704341088917374
1104	28-29	0.4500037169255293
1104	30-31	0.42852251243044037
1104	32-33	0.46192153008907866
1104	34-35	0.4874627183466558
1104	36-37	0.43969605814582025
1104	38-39	0.44021129685643956
1104	40-41	0.5124305928919526
1104	42-43	0.5035535943172604
1104	44-45	0.4508680104397982
1104	46-47	0.45039493061938884
1104	48-49	0.4766887047502735
1104	50-51	0.4676263956408775
1104	52-53	0.4444385131533153
1104	54-55	0.44294481355808557
1104	56-57	0.4206855992467027
1104	58-59	0.4193925756243644
1104	60-61	0.4370379208748929
1104	62-63	0.46810569095082144
1104	64-65	0.43335131416167627
1104	66-67	0.39307081211248374
1104	68-69	0.4290318679397487
1104	70-71	0.4489462161110538
1104	72-73	0.36855671336652307
1104	74-75	0.35423374538844854
1104	76-77	0.3041317090365183
1104	78-79	0.30280092348483123
1104	80-81	0.2507382404261804
1104	82-83	0.27632601568148374
1104	84-85	0.29384670124625956
1104	86-87	0.29956811963789676
1104	88-89	0.3200604039916968
1104	90-91	0.28982224655403854
1104	92-93	0.31783864940547346
1104	94-95	0.29750363323289974
1104	96-97	0.2607483308712233
1104	98-99	0.29953366619346866
1104	100	0.5962052871444286
1105	1	0.21313088132958313
1105	2	0.20267281643403834
1105	3	0.16015982455095923
1105	4	0.18815921010988745
1105	5	0.1531991483124031
1105	6	0.1660652984954183
1105	7	0.2182354376817699
1105	8	0.20313435151484782
1105	9	0.26189247094335144
1105	10-11	0.23506912441668248
1105	12-13	0.2019006733879678
1105	14-15	0.3060145295040968
1105	16-17	0.28925458378972024
1105	18-19	0.2859789660278267
1105	20-21	0.2996346165044699
1105	22-23	0.2967105141906785
1105	24-25	0.2848897978807301
1105	26-27	0.2925743772618503
1105	28-29	0.3447432261792116
1105	30-31	0.37970372901332894
1105	32-33	0.37271295258728543
1105	34-35	0.34723738089657985
1105	36-37	0.3590187104789564
1105	38-39	0.37353730532228724
1105	40-41	0.37952670053977045
1105	42-43	0.37535883276527926
1105	44-45	0.43544505538877587
1105	46-47	0.3842208312188191
1105	48-49	0.47427195621775553
1105	50-51	0.4647259248246627
1105	52-53	0.4293552047879743
1105	54-55	0.3753450635211806
1105	56-57	0.3375585459339092
1105	58-59	0.433233823423258
1105	60-61	0.44459509555864685
1105	62-63	0.4887656920625858
1105	64-65	0.40318389372878016
1105	66-67	0.42863202182845583
1105	68-69	0.36336905088960236
1105	70-71	0.43202350482131635
1105	72-73	0.3819317872672059
1105	74-75	0.43946565754846034
1105	76-77	0.3369188633756366
1105	78-79	0.3403587743429526
1105	80-81	0.3187487195534189
1105	82-83	0.36740141778860647
1105	84-85	0.31461166267397545
1105	86-87	0.3497667479875375
1105	88-89	0.34454860908151375
1105	90-91	0.37442423714239936
1105	92-93	0.30632109632551874
1105	94-95	0.3076478561585603
1105	96-97	0.3480243771196001
1105	98-99	0.3680821041976827
1105	100	0.4389866350277707
1106	1	0.1492916871153156
1106	2	0.20146505377925905
1106	3	0.13288459926384633
1106	4	0.1701111653760492
1106	5	0.13866515460235007
1106	6	0.1689532829574958
1106	7	0.17309955969079738
1106	8	0.0995849392546333
1106	9	0.19130003134087303
1106	10-11	0.21919429418439762
1106	12-13	0.17335998882145986
1106	14-15	0.23668304456491285
1106	16-17	0.1984897224981097
1106	18-19	0.18882486986778702
1106	20-21	0.18425540665918305
1106	22-23	0.25501991675094615
1106	24-25	0.1829617846491658
1106	26-27	0.2341663611473379
1106	28-29	0.2473069908971084
1106	30-31	0.20888602106710863
1106	32-33	0.23570193805014128
1106	34-35	0.2153933862630808
1106	36-37	0.19735618430525648
1106	38-39	0.25881595712071714
1106	40-41	0.22249533264552213
1106	42-43	0.21209119925784847
1106	44-45	0.21848761575250109
1106	46-47	0.24457273933666102
1106	48-49	0.2893026589303602
1106	50-51	0.26990353057615835
1106	52-53	0.20250784295393487
1106	54-55	0.16818156022117847
1106	56-57	0.1948936375690593
1106	58-59	0.12154881170750542
1106	60-61	0.15582510350929368
1106	62-63	0.14870996996135943
1106	64-65	0.1279482084603032
1106	66-67	0.20456517182391565
1106	68-69	0.20504947540462837
1106	70-71	0.27563899979978856
1106	72-73	0.25099626108674755
1106	74-75	0.19443259742009644
1106	76-77	0.22512390148843764
1106	78-79	0.2855862086562624
1106	80-81	0.20128886506753219
1106	82-83	0.23077982731376068
1106	84-85	0.1995805094997607
1106	86-87	0.15867601525917507
1106	88-89	0.15418559708496815
1106	90-91	0.16867323129915945
1106	92-93	0.15497755703363225
1106	94-95	0.20474688338671143
1106	96-97	0.1415209126944248
1106	98-99	0.17850995263536618
1106	100	0.3266641825946408
1107	1	0.11322449428073611
1107	2	0.18325721417861018
1107	3	0.1436852534043851
1107	4	0.1370416691651215
1107	5	0.07502143197417865
1107	6	0.0561907311282539
1107	7	0.11586226215312223
1107	8	0.09266452788222779
1107	9	0.10990043385866954
1107	10-11	0.1667878160448666
1107	12-13	0.043994412803819216
1107	14-15	0.16204500604344219
1107	16-17	0.1740087189674071
1107	18-19	0.132396308263111
1107	20-21	0.17418417685792775
1107	22-23	0.1392804949593156
1107	24-25	0.1355727799474451
1107	26-27	0.1751279176193563
1107	28-29	0.20297629626245595
1107	30-31	0.14396044363482474
1107	32-33	0.2093494494278474
1107	34-35	0.2054757203423705
1107	36-37	0.18410639993979316
1107	38-39	0.24171220891112455
1107	40-41	0.2311292772217115
1107	42-43	0.24439500457702223
1107	44-45	0.2175180298821502
1107	46-47	0.17802667737669253
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2216	72-73	-1.0182563401723073
2216	74-75	-0.8818435129667392
2216	76-77	-1.0418459884703353
2216	78-79	-1.0371551482856418
2216	80-81	-1.0679240144520818
2216	82-83	-1.0038831088362983
2216	84-85	-0.9960455048700183
2216	86-87	-0.9767538082054514
2216	88-89	-0.872078978825293
2216	90-91	-0.9138904849544218
2216	92-93	-0.9370666276281625
2216	94-95	-0.959597819418633
2216	96-97	-0.9636377341902147
2216	98-99	-1.0294928902200944
2216	100	-0.41036783633238016
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	32.0
13	120.0
14	361.0
15	1069.0
16	2459.0
17	5044.0
18	9088.0
19	14253.0
20	20513.0
21	29450.0
22	40611.0
23	48145.0
24	49434.0
25	48582.0
26	50710.0
27	60095.0
28	75564.0
29	95710.0
30	119586.0
31	148790.0
32	186316.0
33	240401.0
34	341149.0
35	491023.0
36	778983.0
37	1443578.0
38	1307723.0
39	536770.0
40	1455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.95643742821725	22.284456533409685	13.557707637196561	25.201398401176505
2	14.021255868254354	20.873349828003366	40.29198429318691	24.813410010555373
3	16.898188065111224	27.45704422630803	29.834456370729228	25.81031133785151
4	11.37470027094676	17.655370244246104	39.52374199277508	31.446187492032056
5	12.151501983724462	39.12786079064927	35.60492167350955	13.115715552116713
6	29.35951556356016	39.71513671911539	17.64744829973683	13.277899417587612
7	25.669886006478592	32.69374929233957	24.260291497533114	17.37607320364873
8	26.279237744468055	35.11008586274325	19.611193716630172	18.99948267615853
9	26.130222449251832	14.312073167160674	21.66420476914017	37.89349961444732
10-11	25.36088218356212	26.195660661588864	27.769976346262094	20.673480808586923
12-13	25.713701689653213	24.046335766010124	28.312375416890283	21.927587127446376
14-15	22.926444830918378	24.99241635970786	26.144641597851248	25.936497211522514
16-17	21.244920302845934	27.306882033896102	27.55342426086136	23.89477340239661
18-19	20.674870868176136	27.438383488045332	30.264894510553336	21.621851133225196
20-21	22.2467716064044	26.682677134611566	29.453450615094795	21.617100643889234
22-23	22.57717560704211	26.31383987688343	29.203703583199665	21.905280932874792
24-25	21.992762346633	26.41603573764198	30.106010717388266	21.485191198336757
26-27	22.047008143783493	26.653858292310744	28.9634652237995	22.335668340106263
28-29	21.233760423750045	27.19038656129311	29.440663931465977	22.13518908349086
30-31	22.197544501238	26.730057816633103	29.343071927535618	21.729325754593283
32-33	22.730615810587896	26.91334174933545	28.58583508757089	21.770207352505764
34-35	22.49970717518407	26.78665434633367	29.360958643375163	21.35267983510709
36-37	22.146568581198693	26.349548236381203	29.108991023292585	22.394892159127515
38-39	22.314105046022302	26.389193462848663	29.790101545138945	21.50659994599009
40-41	21.843673845212802	25.694556061311008	30.259182498186117	22.202587595290073
42-43	22.094356289714366	25.883533809878607	29.331171852377246	22.69093804802978
44-45	22.252554896518966	26.966766010196814	28.933222927532366	21.84745616575186
46-47	22.30558872429242	26.742502871310002	28.195361329554807	22.75654707484277
48-49	23.273708235860628	26.490502715136767	28.082477363014924	22.15331168598768
50-51	22.54541224853669	26.504615244459917	27.88633354047978	23.063638966523616
52-53	21.79033092457809	27.175908000950056	27.72054589052907	23.31321518394279
54-55	22.04298993658716	27.147146096807884	27.46392560948232	23.345938357122638
56-57	23.161702743768526	26.486395033691124	27.26127456881546	23.090627653724894
58-59	22.00555261013509	26.951990875577252	28.208461695010218	22.833994819277443
60-61	22.201139739255773	27.585805805089297	27.3106318183445	22.902422637310437
62-63	22.650243532638576	26.70868160507042	26.91413888355659	23.726935978734414
64-65	21.735919260961502	26.974405784662274	27.439566592820512	23.85010836155571
66-67	22.416312131040012	26.585372916384685	27.32535893885285	23.672956013722448
68-69	21.958143946172292	26.503769306102793	27.72233537551529	23.815751372209622
70-71	21.994921253729164	26.160553877633923	27.825442031420422	24.019082837216494
72-73	22.57148490555632	26.146157221160188	28.281622717564364	23.000735155719127
74-75	22.654798959295356	26.640685382713393	27.666244400307207	23.038271257684045
76-77	22.367426453094083	26.66369232208329	27.185238357657482	23.78364286716515
78-79	22.08964394689881	26.651481527182654	27.431949326327874	23.82692519959066
80-81	21.684303939144495	27.119099986660206	28.010653942886147	23.185942131309154
82-83	22.56539754222371	25.991203867890455	29.039095366535328	22.404303223350503
84-85	22.994345227443542	26.67048423463727	27.362209656060987	22.9729608818582
86-87	21.4991898513426	27.32849413211605	27.89604556354136	23.276270452999988
88-89	22.508841682636465	26.113027125338782	27.672921187827598	23.705210004197156
90-91	22.129373257285646	25.99171631131833	28.024099482350795	23.85481094904523
92-93	22.483463531922784	26.48654144609909	27.504969887848098	23.525025134130033
94-95	21.21165215014755	26.528838363955217	28.166208992324737	24.093300493572496
96-97	21.689704930193816	26.36047222881982	27.717446827893845	24.232376013092523
98-99	20.958562034937252	27.144364261056573	27.666731196579807	24.230342507426364
100	21.873996344884425	26.447237102792677	26.677281163099643	25.001485389223255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	506.0
1	326.0
2	192.0
3	244.5
4	183.0
5	147.0
6	282.5
7	621.0
8	1008.5
9	1117.0
10	936.0
11	767.5
12	821.0
13	959.5
14	1220.0
15	1867.0
16	2560.0
17	3346.5
18	4633.0
19	6199.5
20	7966.0
21	9873.0
22	12106.5
23	14486.5
24	16948.5
25	19559.5
26	23245.0
27	27590.0
28	33746.0
29	46400.0
30	63393.0
31	78144.5
32	100058.0
33	121043.5
34	132763.5
35	150177.0
36	167211.5
37	195638.5
38	207926.5
39	191069.5
40	175349.0
41	159904.0
42	155690.5
43	164349.0
44	179697.0
45	217475.5
46	252277.5
47	274036.5
48	292486.0
49	286115.5
50	268217.0
51	268876.5
52	269422.0
53	257379.0
54	246027.0
55	210973.0
56	180050.0
57	147667.5
58	112842.5
59	109788.5
60	91083.0
61	58563.5
62	40938.5
63	34118.0
64	24753.0
65	10298.5
66	4519.0
67	2850.5
68	1912.0
69	1160.0
70	642.5
71	325.0
72	131.0
73	42.5
74	13.0
75	6.0
76	3.5
77	2.5
78	2.5
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0063120015877612245
3	0.0
4	0.0849680007548373
5	0.004050743286991095
6	1.6268045329281504E-5
7	6.507218131712602E-5
8	0.0
9	0.0
10-11	0.0
12-13	3.090928612563486E-4
14-15	0.009199579633708693
16-17	0.0
18-19	1.1387631730497053E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.6268045329281504E-5
60-61	0.0
62-63	0.0
64-65	9.760827197568905E-5
66-67	1.952165439513781E-4
68-69	0.0
70-71	4.961753825430859E-4
72-73	3.741650425734746E-4
74-75	5.043094052077266E-4
76-77	1.2201033996961129E-4
78-79	2.440206799392226E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06261570647240451
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6147020.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.39250130369791
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03934562698112	42.29851272087606
2	17.02539618871393	20.564125230457588
3	6.259896851700073	11.341524863319332
4	2.8366459411094707	6.852485747863322
5	1.4836772019001114	4.480148867500967
6	0.8091152501550007	2.9318696279896646
7	0.49282521900374826	2.0834063376825354
8	0.3026501193726357	1.4622238183020984
9	0.2037208446552658	1.1072890238790223
>10	0.5367885774611334	4.513037116295961
>50	0.004626583120013108	0.1815537119065411
>100	0.00399031672361859	0.5199653773314786
>500	7.019296092586123E-4	0.29763667272504396
>1k	4.954795956196333E-4	0.47313648817385284
>5k	4.1289966301632674E-5	0.20916353234963933
>10k+	8.257993260326535E-5	0.6839208633468716
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15079	0.24530585552023582	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12348	0.20087782372596805	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8388	0.13645636422201327	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.6268045329281504E-5	0.0
4	0.0	0.0	0.0	1.6268045329281504E-5	0.0
5	0.0	0.0	0.0	1.6268045329281504E-5	0.0
6	0.0	0.0	0.0	1.6268045329281504E-5	0.0
7	0.0	0.0	0.0	3.253609065856301E-5	0.0
8	0.0	0.0	0.0	3.253609065856301E-5	0.0
9	0.0	0.0	0.0	1.7894849862209656E-4	0.0
10-11	0.0	0.0	0.0	2.765567705977856E-4	0.0
12-13	0.0	0.0	0.0	3.578969972441931E-4	0.0
14-15	0.0	0.0	0.0	4.473712465552414E-4	0.0
16-17	0.0	0.0	0.0	5.368454958662896E-4	0.0
18-19	0.0	0.0	0.0	5.693815865248527E-4	0.0
20-21	0.0	0.0	0.0	6.507218131712602E-4	0.0
22-23	0.0	0.0	0.0	6.91391926494464E-4	0.0
24-25	0.0	0.0	0.0	7.5646410781159E-4	0.0
26-27	0.0	0.0	0.0	0.0011143611050557832	0.0
28-29	0.0	0.0	0.0	0.003253609065856301	0.0
30-31	0.0	0.0	0.0	0.011094806914569986	0.0
32-33	0.0	0.0	0.0	0.025337480600355945	0.0
34-35	0.0	0.0	0.0	0.0473237438628799	1.6268045329281504E-5
36-37	0.0	0.0	0.0	0.08447182537229422	1.6268045329281504E-5
38-39	0.0	0.0	0.0	0.1418085511353469	1.6268045329281504E-5
40-41	0.0	0.0	0.0	0.2044242576077514	1.6268045329281504E-5
42-43	0.0	0.0	0.0	0.2707328103699028	1.6268045329281504E-5
44-45	0.0	0.0	0.0	0.3525854804441827	1.6268045329281504E-5
46-47	0.0	0.0	0.0	0.45526124853994293	1.6268045329281504E-5
48-49	0.0	0.0	0.0	0.5741399897836675	1.6268045329281504E-5
50-51	0.0	0.0	0.0	0.7028120943156195	1.6268045329281504E-5
52-53	0.0	0.0	0.0	0.8287267651642585	1.6268045329281504E-5
54-55	0.0	0.0	0.0	0.96465441791307	1.6268045329281504E-5
56-57	0.0	0.0	0.0	1.1351109968732818	1.6268045329281504E-5
58-59	0.0	0.0	0.0	1.3416256983058457	1.6268045329281504E-5
60-61	0.0	0.0	0.0	1.556380164697691	3.253609065856301E-5
62-63	0.0	0.0	0.0	1.7817251285988984	3.253609065856301E-5
64-65	0.0	0.0	0.0	2.01875054904653	3.253609065856301E-5
66-67	0.0	0.0	0.0	2.288800101512603	3.253609065856301E-5
68-69	0.0	0.0	0.0	2.592313023221008	3.253609065856301E-5
70-71	0.0	0.0	0.0	2.9348448516516945	3.253609065856301E-5
72-73	0.0	0.0	0.0	3.288634167450244	3.253609065856301E-5
74-75	0.0	0.0	0.0	3.6354119557118736	3.253609065856301E-5
76-77	0.0	0.0	0.0	4.0434958727969	3.253609065856301E-5
78-79	0.0	0.0	0.0	4.481683807763762	3.253609065856301E-5
80-81	0.0	0.0	0.0	4.957467195486593	3.253609065856301E-5
82-83	0.0	0.0	0.0	5.464135467267067	3.253609065856301E-5
84-85	0.0	0.0	0.0	5.953852435814428	3.253609065856301E-5
86-87	0.0	0.0	0.0	6.476975835445469	3.253609065856301E-5
88	0.0	0.0	0.0	6.899831137689482	3.253609065856301E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11455	0.0	43.4013	1
GTATCAA	20815	0.0	40.877556	1
GGGCACC	6060	0.0	39.88576	94
GTGGTAT	2650	0.0	36.91818	1
GTACTAG	6300	0.0	34.19383	1
CAACGCA	24550	0.0	33.138214	5
ATCAACG	24770	0.0	32.924915	3
TATCAAC	27310	0.0	31.138634	2
CTGTGCG	8455	0.0	30.912172	9
TCCGCAC	360	0.0	30.048786	3
AACGCAG	28570	0.0	29.642681	6
TAGGCAT	8520	0.0	29.401611	5
TAGTACT	9145	0.0	29.212334	4
TATTCTC	3980	0.0	29.16738	5
ACTGTGC	9645	0.0	28.263796	8
CTAACGC	3120	0.0	27.586596	3
ACGCAGA	29795	0.0	27.240927	7
TCAACGC	30290	0.0	27.126593	4
CGCAGAG	29915	0.0	27.102217	8
TACTGTG	10935	0.0	26.990677	7
>>END_MODULE