FastQCFastQC Report
Mon 16 Jan 2017
ERR1146653_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146653_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5454646
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC59800.042.07128594
GTACTAG69050.035.611851
CAACGCA92600.032.9879845
TAGGCAT82350.032.7568175
CTGTGCG84750.032.2158789
GCATCGT69500.032.050228
ATCAACG98900.030.9833683
TAGTACT87150.030.954364
TATTCTC29900.030.6490385
ACTGTGC93000.030.1667758
GTATCAA115900.029.7356221
CTAGGCA94600.029.6592734
GTACTGT94600.029.1106516
CTAACGC30700.029.086573
TAACGCC31550.028.3029384
CGCCTAC31750.028.2703487
TACTGTG105250.027.9954157
TCTAACG32050.027.8613972
CAGTACG7600.027.2064959
CTAGTAC106200.027.127723
Produced by FastQC (version 0.11.5)