##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146653_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5454646
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.158224016737293	31.0	31.0	34.0	28.0	34.0
2	31.21192961009752	31.0	31.0	34.0	27.0	34.0
3	31.35625794964513	31.0	31.0	34.0	28.0	34.0
4	34.80191125143593	37.0	35.0	37.0	32.0	37.0
5	34.956037293712555	37.0	35.0	37.0	32.0	37.0
6	35.01775715600976	37.0	35.0	37.0	32.0	37.0
7	34.963798200653166	37.0	35.0	37.0	32.0	37.0
8	34.91279910740312	37.0	35.0	37.0	32.0	37.0
9	36.38841017363913	39.0	37.0	39.0	31.0	39.0
10-11	36.224902037639104	39.0	37.0	39.0	30.5	39.0
12-13	36.13615778182489	39.0	37.0	39.0	30.0	39.0
14-15	37.26413611442429	40.0	37.0	41.0	30.5	41.0
16-17	37.291470610558406	40.0	37.0	41.0	30.5	41.0
18-19	37.25094900750663	40.0	37.0	41.0	30.0	41.0
20-21	37.32349129531046	40.0	37.0	41.0	30.0	41.0
22-23	37.26183028192847	40.0	37.0	41.0	30.0	41.0
24-25	37.20732894490311	40.0	36.0	41.0	30.0	41.0
26-27	37.245508507793176	40.0	36.0	41.0	30.0	41.0
28-29	37.239353479584196	40.0	36.0	41.0	30.0	41.0
30-31	37.10510636253939	40.0	36.0	41.0	30.0	41.0
32-33	37.09815339070583	40.0	36.0	41.0	30.0	41.0
34-35	37.03519320960517	40.0	36.0	41.0	30.0	41.0
36-37	36.913360922047005	40.0	36.0	41.0	30.0	41.0
38-39	36.804101036070904	40.0	35.0	41.0	30.0	41.0
40-41	36.71558988429314	39.0	35.0	41.0	30.0	41.0
42-43	36.55430746559905	39.0	35.0	41.0	29.0	41.0
44-45	36.38380978343966	39.0	35.0	41.0	29.0	41.0
46-47	36.38913066035816	39.0	35.0	41.0	29.0	41.0
48-49	36.25041597566552	39.0	35.0	41.0	28.0	41.0
50-51	36.064970119050805	39.0	35.0	40.5	28.0	41.0
52-53	35.84631908285157	38.0	34.0	40.0	28.0	41.0
54-55	35.634118786077046	38.0	34.0	40.0	28.0	41.0
56-57	35.38564638291834	37.5	34.0	40.0	27.5	41.0
58-59	35.07227077614203	37.0	33.5	40.0	27.0	41.0
60-61	34.83008384045454	36.5	33.0	40.0	26.5	41.0
62-63	34.52270743142635	36.0	33.0	39.5	26.0	41.0
64-65	34.36885748406038	35.5	33.0	39.0	26.0	41.0
66-67	34.117609190403925	35.0	33.0	39.0	26.0	41.0
68-69	33.99399851062745	35.0	33.0	39.0	26.0	41.0
70-71	33.929989040535354	35.0	33.0	38.0	26.0	41.0
72-73	33.71310631340695	35.0	33.0	37.5	26.0	40.0
74-75	33.46278548598754	35.0	33.0	37.0	26.0	39.5
76-77	33.2044633143929	35.0	32.5	36.5	26.0	39.0
78-79	32.95246547621972	35.0	32.0	36.0	25.0	39.0
80-81	32.77322634686101	35.0	32.0	36.0	25.0	38.5
82-83	32.509930983605535	35.0	32.0	35.5	25.0	37.0
84-85	32.33408639167418	35.0	32.0	35.0	25.0	37.0
86-87	32.16003687498694	35.0	32.0	35.0	25.0	36.5
88-89	32.03561404351447	35.0	32.0	35.0	25.0	36.0
90-91	31.881360953579755	35.0	32.0	35.0	24.0	36.0
92-93	31.72513789162486	35.0	31.5	35.0	24.0	36.0
94-95	31.65513444868833	35.0	31.0	35.0	24.0	35.5
96-97	31.547969015771145	35.0	31.0	35.0	24.0	35.0
98-99	31.453914424510774	35.0	31.0	35.0	23.0	35.0
100	30.45772007935987	34.0	29.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5679884614124049
1101	2	0.5913214613436963
1101	3	0.6146476785085504
1101	4	0.6880809256246749
1101	5	0.5924108235154435
1101	6	0.5359124617013507
1101	7	0.6324411638072149
1101	8	0.607591242127576
1101	9	0.8368405186901029
1101	10-11	0.8459314235389428
1101	12-13	0.7520557961324741
1101	14-15	0.9390723496037552
1101	16-17	0.9345370962374133
1101	18-19	0.9615173733958784
1101	20-21	0.8812192378893684
1101	22-23	0.948887202333033
1101	24-25	0.9925024192795817
1101	26-27	0.894923967752959
1101	28-29	0.9249165959178498
1101	30-31	0.9989018354534451
1101	32-33	0.9462904458670565
1101	34-35	0.9579560214767824
1101	36-37	0.9819354371305664
1101	38-39	0.9161953689144013
1101	40-41	0.9430998814503084
1101	42-43	0.9061152036060207
1101	44-45	0.9092691435478244
1101	46-47	0.9532698237198289
1101	48-49	0.9176864038480446
1101	50-51	0.8290678451541567
1101	52-53	0.8327144792815133
1101	54-55	0.834104838713948
1101	56-57	0.8010122297279523
1101	58-59	0.777532836797775
1101	60-61	0.8023212029507789
1101	62-63	0.7864130698022294
1101	64-65	0.8025803674058238
1101	66-67	0.8479743552027657
1101	68-69	0.8209245059597734
1101	70-71	0.8073493592722585
1101	72-73	0.8311660603421345
1101	74-75	0.8124970018692608
1101	76-77	0.8271469485000793
1101	78-79	0.7995191730728948
1101	80-81	0.7139513902253185
1101	82-83	0.7135422441803385
1101	84-85	0.6252498424598514
1101	86-87	0.6878253475410858
1101	88-89	0.728400306082527
1101	90-91	0.7707283638050413
1101	92-93	0.702975121621769
1101	94-95	0.731535012923942
1101	96-97	0.7130305455044486
1101	98-99	0.7538847179003163
1101	100	0.7737272324508417
1102	1	0.4800596676084119
1102	2	0.5756325324673099
1102	3	0.581544391828615
1102	4	0.6443926874414387
1102	5	0.5761870025835663
1102	6	0.5995825158464783
1102	7	0.577742652551251
1102	8	0.5725908410671963
1102	9	0.6396641023048701
1102	10-11	0.8194243749521632
1102	12-13	0.6932763868489715
1102	14-15	0.9231548755808134
1102	16-17	0.8867286837687445
1102	18-19	0.9453991123462657
1102	20-21	0.8856849563149467
1102	22-23	0.9623183390803973
1102	24-25	0.9392295690126176
1102	26-27	0.876006700384778
1102	28-29	0.9001088776985284
1102	30-31	0.9191633070644869
1102	32-33	0.8457571738584235
1102	34-35	0.9428754201944187
1102	36-37	0.9281308953884846
1102	38-39	0.9613285308388697
1102	40-41	0.8835888827151308
1102	42-43	0.93843780551191
1102	44-45	0.8724514450496059
1102	46-47	0.8856524661426945
1102	48-49	0.899406624917269
1102	50-51	0.9088692787770825
1102	52-53	0.8485845372989544
1102	54-55	0.8582799681497661
1102	56-57	0.8299354991721728
1102	58-59	0.8022018236876391
1102	60-61	0.7941644053329568
1102	62-63	0.7880046868105026
1102	64-65	0.8163259117830535
1102	66-67	0.8683672481757583
1102	68-69	0.7876522602468725
1102	70-71	0.7103599792720914
1102	72-73	0.7460855459891107
1102	74-75	0.7124527074174551
1102	76-77	0.7378960186524495
1102	78-79	0.6867377541410207
1102	80-81	0.7077561137287631
1102	82-83	0.7051263576057423
1102	84-85	0.7307670898342877
1102	86-87	0.7088858578716426
1102	88-89	0.7341198815038084
1102	90-91	0.6892881737616889
1102	92-93	0.7121711792970089
1102	94-95	0.707558991197935
1102	96-97	0.7399196287989547
1102	98-99	0.6532009395875242
1102	100	0.7504670343546742
1103	1	0.3255726026719188
1103	2	0.3896466470755975
1103	3	0.39931143238872124
1103	4	0.4749403935475769
1103	5	0.3481565150820245
1103	6	0.38765417286470694
1103	7	0.40766474826587284
1103	8	0.46728210405724013
1103	9	0.5144338554252315
1103	10-11	0.6124336574852123
1103	12-13	0.6032852448899035
1103	14-15	0.7221216938471287
1103	16-17	0.6892945209575245
1103	18-19	0.7046526395255484
1103	20-21	0.6572669602057459
1103	22-23	0.7252049231003852
1103	24-25	0.7059745883487665
1103	26-27	0.6665540841125548
1103	28-29	0.6774558494365834
1103	30-31	0.6010257359448801
1103	32-33	0.6318464494267886
1103	34-35	0.6330768117561547
1103	36-37	0.676810535084023
1103	38-39	0.7005483293867627
1103	40-41	0.6538174702462527
1103	42-43	0.6314474480614081
1103	44-45	0.5683914578007361
1103	46-47	0.558006328922751
1103	48-49	0.5957768164067474
1103	50-51	0.5586593564620799
1103	52-53	0.5704913496609976
1103	54-55	0.5743095120809372
1103	56-57	0.5569100339000101
1103	58-59	0.549857093870159
1103	60-61	0.5752842266600737
1103	62-63	0.5227348757602286
1103	64-65	0.48793085853989737
1103	66-67	0.49587357628296047
1103	68-69	0.5430752942952637
1103	70-71	0.5266203919294838
1103	72-73	0.4830680468989712
1103	74-75	0.4245050515172437
1103	76-77	0.40062369632384076
1103	78-79	0.44256052917394584
1103	80-81	0.42699017220066793
1103	82-83	0.3667531622699798
1103	84-85	0.38299828355717125
1103	86-87	0.3546091407241292
1103	88-89	0.44915643462783095
1103	90-91	0.4925037588080414
1103	92-93	0.35607445629759127
1103	94-95	0.35615270969648805
1103	96-97	0.39250997983462454
1103	98-99	0.370491891130623
1103	100	0.5867603574242679
1104	1	0.3476839014298214
1104	2	0.385103886955644
1104	3	0.391997747490052
1104	4	0.49251703904561595
1104	5	0.3922954498017006
1104	6	0.3755415510672435
1104	7	0.3945501185613054
1104	8	0.4287388484459953
1104	9	0.4956434508345424
1104	10-11	0.6485218315868408
1104	12-13	0.49712639842475426
1104	14-15	0.6139807200900051
1104	16-17	0.6679715332747591
1104	18-19	0.5852403739110841
1104	20-21	0.5726248553795088
1104	22-23	0.6395450830584579
1104	24-25	0.6068471459139602
1104	26-27	0.6078910609930901
1104	28-29	0.5683736692230283
1104	30-31	0.5291877680817691
1104	32-33	0.5761571847254814
1104	34-35	0.6953237155841521
1104	36-37	0.6228094429278386
1104	38-39	0.5786328275137578
1104	40-41	0.6563062762938472
1104	42-43	0.665143774162118
1104	44-45	0.6614799380174006
1104	46-47	0.5607046162350642
1104	48-49	0.5252771290072715
1104	50-51	0.44529237641663144
1104	52-53	0.49695256255103715
1104	54-55	0.5411973663182934
1104	56-57	0.5514143934450928
1104	58-59	0.5370584695718961
1104	60-61	0.4956262194170762
1104	62-63	0.5579275660963319
1104	64-65	0.4679737146450762
1104	66-67	0.44797549053961916
1104	68-69	0.4541944366024353
1104	70-71	0.4838948071978848
1104	72-73	0.46908355110675615
1104	74-75	0.383175792367517
1104	76-77	0.3668929971363468
1104	78-79	0.40490902817258956
1104	80-81	0.387980285722648
1104	82-83	0.44726482188043803
1104	84-85	0.44150792488374435
1104	86-87	0.4883337435528361
1104	88-89	0.4536951413177448
1104	90-91	0.4928932123667593
1104	92-93	0.4570351840731277
1104	94-95	0.5019031909197054
1104	96-97	0.4706049844522937
1104	98-99	0.42399554635170134
1104	100	0.48739230338199135
1105	1	0.15421439825170324
1105	2	0.24615289297726406
1105	3	0.23531078929811144
1105	4	0.2759050610705742
1105	5	0.18518951582878884
1105	6	0.2017625842033297
1105	7	0.21618393942139846
1105	8	0.2330525738083864
1105	9	0.34981519635765324
1105	10-11	0.3437908252062769
1105	12-13	0.2763777869352211
1105	14-15	0.4285630512318832
1105	16-17	0.3677316211329966
1105	18-19	0.3923988292347431
1105	20-21	0.3557236270241262
1105	22-23	0.35354688189706707
1105	24-25	0.3501050590438908
1105	26-27	0.37289337825529145
1105	28-29	0.42078028127487954
1105	30-31	0.4732240933667171
1105	32-33	0.4091646836162752
1105	34-35	0.4113759329639919
1105	36-37	0.38879670526451093
1105	38-39	0.3934677163351381
1105	40-41	0.3969737282006065
1105	42-43	0.3729909224195396
1105	44-45	0.3196789559675537
1105	46-47	0.37370582060302837
1105	48-49	0.20522087700560832
1105	50-51	0.31671379243874753
1105	52-53	0.30068346876677765
1105	54-55	0.2228939173021942
1105	56-57	0.26287415673453296
1105	58-59	0.28605263871878606
1105	60-61	0.35973424511837493
1105	62-63	0.27467444610321223
1105	64-65	0.2832296596774029
1105	66-67	0.28107275210713567
1105	68-69	0.19302163141802708
1105	70-71	0.22672832347868166
1105	72-73	0.2352256506658108
1105	74-75	0.2194191203254121
1105	76-77	0.20559250895774994
1105	78-79	0.22022037891156998
1105	80-81	0.16816037165146724
1105	82-83	0.28758256057147946
1105	84-85	0.16681661614880028
1105	86-87	0.19793087019116484
1105	88-89	0.20511885070155955
1105	90-91	0.1411806925890957
1105	92-93	0.18193615452037193
1105	94-95	0.1748806218939727
1105	96-97	0.1758571358232892
1105	98-99	0.21087735989563683
1105	100	0.2216766376465742
1106	1	0.12146938639481064
1106	2	0.25588490585179713
1106	3	0.17434090776348654
1106	4	0.3124726145095238
1106	5	0.20966420522447748
1106	6	0.2558038287580544
1106	7	0.15806887007146742
1106	8	0.21648200612175827
1106	9	0.31069908133266466
1106	10-11	0.3138263155925145
1106	12-13	0.21236880926895196
1106	14-15	0.31155382209930593
1106	16-17	0.24713321659177012
1106	18-19	0.32757087937462614
1106	20-21	0.28814033070626976
1106	22-23	0.27763254135476956
1106	24-25	0.35230106036866005
1106	26-27	0.39014510732809526
1106	28-29	0.38212393449180126
1106	30-31	0.4694919085932341
1106	32-33	0.3568767914181663
1106	34-35	0.36056517645216246
1106	36-37	0.37891556245974556
1106	38-39	0.38696344509595804
1106	40-41	0.3909647781030401
1106	42-43	0.3662776364872826
1106	44-45	0.34269946833629916
1106	46-47	0.3126691817476157
1106	48-49	0.21216707791572986
1106	50-51	0.30504356128382426
1106	52-53	0.24920768928269155
1106	54-55	0.36007910641275487
1106	56-57	0.34487326130103924
1106	58-59	0.32011267628649875
1106	60-61	0.2741257398132788
1106	62-63	0.35458771952232127
1106	64-65	0.3164029410971807
1106	66-67	0.33269546439541386
1106	68-69	0.3215616395923817
1106	70-71	0.2557542221542377
1106	72-73	0.24881208306722868
1106	74-75	0.27592785513077445
1106	76-77	0.25158883918182084
1106	78-79	0.27377989050300755
1106	80-81	0.253380279904718
1106	82-83	0.2858415474316729
1106	84-85	0.2600912342529895
1106	86-87	0.2629849278618508
1106	88-89	0.29124143118843193
1106	90-91	0.21247597450678057
1106	92-93	0.31318332931707715
1106	94-95	0.2758247988848197
1106	96-97	0.30390652444419786
1106	98-99	0.30118375880029546
1106	100	0.38915365583893546
1107	1	0.08444252671135644
1107	2	0.1983477285721662
1107	3	0.13815309883860394
1107	4	0.24365827877924318
1107	5	0.13991345614569894
1107	6	0.256117060022639
1107	7	0.1603405015228745
1107	8	0.17280268538100074
1107	9	0.2323831153300162
1107	10-11	0.3520547728989385
1107	12-13	0.2572989599399307
1107	14-15	0.35118775798252244
1107	16-17	0.24889459144278447
1107	18-19	0.23751730086183898
1107	20-21	0.2594179373989931
1107	22-23	0.3524722156798532
1107	24-25	0.37202234118211663
1107	26-27	0.22442099843766528
1107	28-29	0.22287515542932113
1107	30-31	0.3684740697241651
1107	32-33	0.34614466741329153
1107	34-35	0.34536005642547707
1107	36-37	0.3351435819548598
1107	38-39	0.3743706743284889
1107	40-41	0.2951131215288001
1107	42-43	0.3483942827901956
1107	44-45	0.35264946884053217
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2216	80-81	-1.3642522088360174
2216	82-83	-1.3540699887351302
2216	84-85	-1.3859795081658746
2216	86-87	-1.3711662705734327
2216	88-89	-1.4072015402032463
2216	90-91	-1.5094216628167452
2216	92-93	-1.6074038507329291
2216	94-95	-1.6290337151655692
2216	96-97	-1.546406852278409
2216	98-99	-1.523008990898738
2216	100	-1.110648246629907
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20.0
3	29.0
4	43.0
5	38.0
6	55.0
7	161.0
8	541.0
9	942.0
10	1610.0
11	3112.0
12	6327.0
13	9286.0
14	12669.0
15	15969.0
16	19259.0
17	22181.0
18	25378.0
19	28291.0
20	32092.0
21	36421.0
22	41133.0
23	47731.0
24	55051.0
25	63221.0
26	70637.0
27	77816.0
28	87518.0
29	104966.0
30	121906.0
31	141494.0
32	173604.0
33	223623.0
34	320989.0
35	449404.0
36	717171.0
37	1182784.0
38	988042.0
39	372672.0
40	460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.05329988066068	20.959384644959115	11.612342759537333	24.37497271484287
2	15.70514389518708	20.0020057889195	40.26679926141498	24.026051054478437
3	18.37287347521288	27.117504222430988	28.96182467689144	25.547797625464685
4	11.837355831331022	16.44307096568371	41.09060773379366	30.62896546919161
5	12.651755114971705	39.05844223107497	35.601453424363946	12.688349229589384
6	29.28096877464718	39.23878691966491	18.302154669979416	13.178089635708496
7	26.501974020116947	31.96851101042147	24.34603422245974	17.183480747001845
8	26.255788504336095	35.0630600702788	20.039687886889524	18.641463538495582
9	26.406282684503076	14.587383041613784	21.54424944326919	37.462084830613954
10-11	25.631201685985015	25.884519266298422	27.9116190682918	20.572659979424763
12-13	25.611393552155427	23.917254500718563	27.85593667323662	22.61541527388939
14-15	23.040199199359957	25.096587563313115	25.13307945754767	26.73013377977926
16-17	21.84209751983344	27.582339243651273	26.569581877799408	24.005981358715882
18-19	21.08705570083157	28.705736916060776	28.138514209992575	22.06869317311508
20-21	23.02209843665725	27.673416206595764	27.00620797808233	22.298277378664665
22-23	23.59595107379447	27.4025764027381	26.53379305691985	22.467679466547573
24-25	22.924235246494316	27.346876966241872	27.69993884025279	22.028948947011017
26-27	22.920888571593835	27.531023123457505	26.432541785457513	23.115546519491144
28-29	21.918486318271484	28.245825693872085	26.92956955285444	22.906118435001993
30-31	22.81853773119637	27.927399545243368	26.988001861268064	22.266060862292196
32-33	23.399612084952626	27.95669718150145	25.936075604382385	22.707615129163536
34-35	23.18020956532054	27.661454453652652	27.19102058820779	21.967315392819014
36-37	22.701482057802153	27.269287850081575	26.82603736116394	23.203192730952335
38-39	23.040099284053653	27.371941822610058	27.49211417629501	22.09584471704127
40-41	22.56896090996637	26.428929274187595	28.114361827688132	22.887747988157898
42-43	22.50747117579718	26.737618503524196	27.340180738416297	23.41472958226233
44-45	22.62524394897439	27.89710895595982	27.221771644873005	22.255875450192786
46-47	22.594140609886622	27.538689786930437	26.73048540121693	23.136684201966016
48-49	23.42078609102961	27.08442611460022	26.935473208785375	22.559314585584794
50-51	22.89672426532306	26.786359505208562	27.00642634032145	23.310489889146936
52-53	22.26476999994132	27.59333208147266	26.789292776959506	23.35260514162652
54-55	22.271828148243227	27.44546085912126	26.796725978321412	23.485985014314103
56-57	23.6304106130073	26.750422942114895	26.442103572388266	23.177062872489543
58-59	22.309719467686858	27.26415166993299	27.574522813420487	22.851606048959667
60-61	22.354851476942645	28.031321032422756	26.629689429160724	22.98413806147387
62-63	22.851859141839192	27.161255195656658	26.217490446610803	23.76939521589335
64-65	21.80994659565033	27.485285223672022	26.784317036632032	23.92045114404561
66-67	22.665351626184524	26.84848953243728	26.771295996048277	23.714862845329915
68-69	21.984834012001517	27.02621413157668	27.069895183808768	23.919056672613035
70-71	22.018506403975568	26.684490527513923	27.222754134861916	24.074248933648594
72-73	22.720352188739486	26.681292915943743	27.80202346079231	22.796331434524465
74-75	22.885771638620948	26.970085178600765	27.170857022577387	22.973286160200896
76-77	22.576000752474073	26.887481015110264	26.878588473046932	23.657929759368727
78-79	22.346737216572972	26.873346257377957	26.931167085967754	23.84874944008132
80-81	21.843097841696675	27.27014036136035	27.72426331760765	23.162498479335326
82-83	22.808089782660748	26.05260349841719	28.887344116630043	22.251962602292018
84-85	23.070333000275305	26.685227340754853	27.335996880823988	22.908442778145858
86-87	21.492652604460645	27.46476465168111	27.91228320913416	23.13029953472408
88-89	22.935868545952363	26.019503078794195	27.547353953112342	23.4972744221411
90-91	22.395059799760464	26.0926373722283	27.818100841457845	23.694201986553395
92-93	22.832029926813846	26.316249291474104	27.702828630652633	23.148892151059417
94-95	21.445172018968886	26.46055925759897	28.399332990477973	23.694935732954175
96-97	21.822780716101462	26.34775640628972	27.890273205889137	23.939189671719678
98-99	21.137321320935904	27.11932105966397	27.729899822839293	24.013457796560836
100	22.054426477678334	26.31390593141006	26.79136406605524	24.84030352485637
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	335.0
1	239.5
2	162.0
3	186.0
4	180.0
5	168.5
6	149.0
7	143.0
8	177.0
9	180.0
10	180.0
11	199.0
12	198.5
13	223.0
14	253.5
15	411.0
16	529.5
17	610.0
18	833.0
19	1074.0
20	1429.5
21	1910.5
22	2718.5
23	3863.0
24	5435.5
25	7467.0
26	10587.5
27	13982.0
28	18992.0
29	30555.0
30	47951.5
31	64207.5
32	84122.0
33	103607.0
34	118680.5
35	136657.5
36	151369.0
37	176599.5
38	185929.0
39	170438.5
40	157342.5
41	143345.0
42	140643.5
43	151389.5
44	167255.0
45	200545.0
46	232674.5
47	253122.5
48	266632.0
49	261016.5
50	244727.0
51	249293.0
52	253561.0
53	239668.5
54	224373.0
55	189256.5
56	162208.5
57	131924.0
58	99699.5
59	97286.0
60	80786.5
61	52008.5
62	37155.5
63	31879.0
64	23301.5
65	9504.5
66	3987.0
67	2375.5
68	1639.5
69	1101.5
70	685.0
71	453.5
72	285.5
73	166.5
74	96.5
75	67.5
76	56.0
77	47.0
78	34.5
79	30.5
80	24.5
81	18.0
82	17.0
83	16.5
84	13.5
85	16.0
86	17.0
87	15.0
88	12.0
89	10.5
90	11.0
91	9.5
92	7.0
93	7.5
94	7.0
95	5.5
96	6.0
97	8.0
98	6.5
99	5.0
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05433899835113039
2	0.007938187006086188
3	0.008103183964642251
4	0.009734820554807774
5	0.003978260000740653
6	0.00307994322637986
7	7.516528111998469E-4
8	0.0015583046085850484
9	6.416548388291376E-4
10-11	5.591563595511056E-4
12-13	3.116609217170097E-4
14-15	1.8332995395118216E-4
16-17	5.95822350341342E-4
18-19	0.0015858041016777258
20-21	9.533157605461473E-4
22-23	0.0011733117052875658
24-25	0.001429973640819221
26-27	0.003785763549091912
28-29	0.004335753410945458
30-31	0.005499898618535465
32-33	0.006581545346847439
34-35	0.0039049280191601806
36-37	0.009285662167627377
38-39	0.0072690326741643736
40-41	0.006939038757052245
42-43	0.01562887857433828
44-45	0.021064611708990832
46-47	0.019707970049752083
48-49	0.02281541276922462
50-51	0.01971713654744964
52-53	0.02621618341501905
54-55	0.02032212539548854
56-57	0.01623386742237718
58-59	0.01858965733064987
60-61	0.017058852215157502
62-63	0.012677266315724245
64-65	0.01264060032493401
66-67	0.015060555717089615
68-69	0.00615988645275972
70-71	0.009789819540993129
72-73	0.009624822582437064
74-75	0.012723098804212043
76-77	0.011687284564387862
78-79	0.013364753643041181
80-81	0.01130229166109038
82-83	0.007479862121208233
84-85	0.011760616545968336
86-87	0.010458973872914942
88-89	0.008827337282749422
90-91	0.014263070417401972
92-93	0.009908984011061396
94-95	0.008268180923198316
96-97	0.010495639863705178
98-99	0.010238977928173524
100	0.25976021175343
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5454646.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.10001969813011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.473656340019	49.22710151067029
2	14.8237169747961	19.596959680670558
3	5.302254416185686	10.514373640631137
4	2.357682132394377	6.233713415727909
5	1.1965781408096527	3.954691933893497
6	0.649612573591844	2.576364234634433
7	0.38055558426664005	1.7608312131380828
8	0.2405276290174579	1.2719104812798798
9	0.1584447024282375	0.9425878156413776
>10	0.4148346478510199	3.7785551466071086
>50	0.0016388567202755835	0.06847620531000494
>100	4.572326316567826E-4	0.05877763896048097
>500	4.0769288095210904E-5	0.01565708283526038
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.8332995395118215E-5
7	0.0	0.0	0.0	0.0	1.8332995395118215E-5
8	0.0	0.0	0.0	0.0	1.8332995395118215E-5
9	0.0	0.0	0.0	0.0	3.666599079023643E-5
10-11	0.0	0.0	0.0	0.0	3.666599079023643E-5
12-13	0.0	0.0	0.0	0.0	3.666599079023643E-5
14-15	0.0	0.0	0.0	0.0	1.283309677658275E-4
16-17	0.0	0.0	0.0	0.0	1.6499695855606394E-4
18-19	0.0	0.0	0.0	0.0	2.0166294934630038E-4
20-21	0.0	0.0	0.0	2.749949309267732E-5	2.0166294934630038E-4
22-23	0.0	0.0	0.0	1.4666396316094572E-4	2.4749543783409594E-4
24-25	0.0	0.0	0.0	3.116609217170097E-4	3.116609217170097E-4
26-27	0.0	0.0	0.0	7.699858065949651E-4	3.116609217170097E-4
28-29	0.0	0.0	0.0	0.003464936129677343	3.208274194145688E-4
30-31	0.0	0.0	0.0	0.013282255163763149	3.39160414809687E-4
32-33	0.0	0.0	0.0	0.030350273876618206	3.4832691250724613E-4
34-35	0.0	0.0	0.0	0.05645645931926655	3.666599079023643E-4
36-37	0.0	0.0	0.0	0.10242644527252548	3.666599079023643E-4
38-39	0.0	0.0	0.0	0.17267848362661847	3.7582640559992344E-4
40-41	0.0	0.0	0.0	0.24680795050677898	3.8499290329748257E-4
42-43	0.0	0.0	0.0	0.3255573322265093	3.8499290329748257E-4
44-45	0.0	0.0	0.0	0.4206689123363826	3.941594009950417E-4
46-47	0.0	0.0	0.0	0.5398425489023486	4.124923963901599E-4
48-49	0.0	0.0	0.0	0.6775966763012669	4.399918894828372E-4
50-51	0.0	0.0	0.0	0.8260847725040268	4.399918894828372E-4
52-53	0.0	0.0	0.0	0.9727487356649726	4.7665788027307363E-4
54-55	0.0	0.0	0.0	1.1295508452794185	5.1332387106331E-4
56-57	0.0	0.0	0.0	1.32437558734334	5.1332387106331E-4
58-59	0.0	0.0	0.0	1.559853746695936	5.1332387106331E-4
60-61	0.0	0.0	0.0	1.802041782363145	5.1332387106331E-4
62-63	0.0	0.0	0.0	2.0607295138859607	5.316568664584283E-4
64-65	0.0	0.0	0.0	2.329124566470491	5.316568664584283E-4
66-67	0.0	0.0	0.0	2.633030631135366	5.499898618535465E-4
68-69	0.0	0.0	0.0	2.9792675821675685	5.683228572486648E-4
70-71	0.0	0.0	0.0	3.3588980843119796	6.141553457364602E-4
72-73	0.0	0.0	0.0	3.7559907645702397	7.333198158047286E-4
74-75	0.0	0.0	0.0	4.144155276071078	7.333198158047286E-4
76-77	0.0	0.0	0.0	4.58491715136051	7.333198158047286E-4
78-79	0.0	0.0	0.0	5.0613275361957495	7.333198158047286E-4
80-81	0.0	0.0	0.0	5.587512003528735	7.60819308897406E-4
82-83	0.0	0.0	0.0	6.136026059252975	8.066517973852015E-4
84-85	0.0	0.0	0.0	6.665510465757081	8.158182950827606E-4
86-87	0.0	0.0	0.0	7.226078465953611	8.249847927803198E-4
88	0.0	0.0	0.0	7.681745066499274	8.249847927803198E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5980	0.0	42.071285	94
GTACTAG	6905	0.0	35.61185	1
CAACGCA	9260	0.0	32.987984	5
TAGGCAT	8235	0.0	32.756817	5
CTGTGCG	8475	0.0	32.215878	9
GCATCGT	6950	0.0	32.05022	8
ATCAACG	9890	0.0	30.983368	3
TAGTACT	8715	0.0	30.95436	4
TATTCTC	2990	0.0	30.649038	5
ACTGTGC	9300	0.0	30.166775	8
GTATCAA	11590	0.0	29.735622	1
CTAGGCA	9460	0.0	29.659273	4
GTACTGT	9460	0.0	29.110651	6
CTAACGC	3070	0.0	29.08657	3
TAACGCC	3155	0.0	28.302938	4
CGCCTAC	3175	0.0	28.270348	7
TACTGTG	10525	0.0	27.995415	7
TCTAACG	3205	0.0	27.861397	2
CAGTACG	760	0.0	27.206495	9
CTAGTAC	10620	0.0	27.12772	3
>>END_MODULE