FastQCFastQC Report
Mon 16 Jan 2017
ERR1146653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5454646
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121610.22294755700003263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101860.18673989109467415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66010.12101610260317536No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC61750.044.55035494
GGTATCA106550.039.687161
GTATCAA184750.039.2594071
CTGTGCG82650.036.0012749
CAACGCA204500.033.5534325
ACTGTGC90050.033.506788
ATCAACG208550.032.949743
TAGTACT90750.032.9108434
GTGGTAT25000.032.361771
GTACTAG64600.031.9650821
GTACTGT93850.031.8991096
TACTGTG105700.031.0354357
TATCAAC235000.030.804532
AACGCAG239950.029.7907816
CTAGTAC103800.029.5435183
CAGTACG8600.028.9690659
TATTCTC33150.028.9217155
CTAACGC31150.028.839473
TAACGCC32100.028.4255334
TCTAACG32350.027.7717272
Produced by FastQC (version 0.11.5)