##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146653_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5454646
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08236226512225	33.0	31.0	34.0	30.0	34.0
2	32.14577004630548	33.0	31.0	34.0	30.0	34.0
3	32.304912912771975	34.0	31.0	34.0	30.0	34.0
4	35.925431824540034	37.0	35.0	37.0	35.0	37.0
5	35.83466076441991	37.0	35.0	37.0	35.0	37.0
6	35.87172036462128	37.0	35.0	37.0	35.0	37.0
7	35.795086610570145	37.0	35.0	37.0	33.0	37.0
8	35.797564864887654	37.0	35.0	37.0	33.0	37.0
9	37.49366081685228	39.0	37.0	39.0	34.0	39.0
10-11	37.42912179818819	39.0	37.0	39.0	34.0	39.0
12-13	37.40164439635496	39.0	37.0	39.0	34.0	39.0
14-15	38.60705415163514	40.0	38.0	41.0	34.0	41.0
16-17	38.61093588841512	40.0	38.0	41.0	34.0	41.0
18-19	38.661046748038274	40.0	38.0	41.0	34.0	41.0
20-21	38.657547529207214	40.0	38.0	41.0	34.0	41.0
22-23	38.61737883631679	40.0	38.0	41.0	34.0	41.0
24-25	38.54264676387798	40.0	38.0	41.0	34.0	41.0
26-27	38.396141106132276	40.0	38.0	41.0	34.0	41.0
28-29	38.33884132902483	40.0	38.0	41.0	34.0	41.0
30-31	38.20619459081304	40.0	37.5	41.0	33.5	41.0
32-33	38.06281883370616	40.0	37.0	41.0	33.0	41.0
34-35	37.96305498101985	40.0	37.0	41.0	33.0	41.0
36-37	37.95652256810067	40.0	37.0	41.0	33.0	41.0
38-39	37.853889601635004	40.0	37.0	41.0	33.0	41.0
40-41	37.736670537373094	40.0	37.0	41.0	33.0	41.0
42-43	37.59722335784944	40.0	36.5	41.0	32.5	41.0
44-45	37.38789015822475	40.0	36.0	41.0	32.0	41.0
46-47	37.13589103674189	40.0	36.0	41.0	31.0	41.0
48-49	36.86538823967678	39.0	35.0	41.0	31.0	41.0
50-51	36.651761085870646	39.0	35.0	41.0	30.0	41.0
52-53	36.466247305508006	39.0	35.0	41.0	30.0	41.0
54-55	36.28281202849827	39.0	35.0	41.0	30.0	41.0
56-57	36.044995953907915	38.0	35.0	40.0	30.0	41.0
58-59	35.76194101322066	37.5	35.0	40.0	29.0	41.0
60-61	35.58254825336053	37.0	35.0	40.0	29.0	41.0
62-63	35.36667228267426	36.5	34.5	40.0	29.0	41.0
64-65	35.12447874710843	36.0	34.0	39.5	29.0	41.0
66-67	34.88130677957837	35.5	34.0	39.0	29.0	41.0
68-69	34.63417772665724	35.0	34.0	39.0	29.0	41.0
70-71	34.43072575195531	35.0	34.0	38.5	29.0	41.0
72-73	34.19999033851143	35.0	34.0	37.5	28.5	40.0
74-75	33.95060770213136	35.0	34.0	37.0	28.0	39.5
76-77	33.71173803029564	35.0	33.5	36.5	28.0	39.0
78-79	33.48783569089544	35.0	33.0	36.0	28.0	39.0
80-81	33.36562840191645	35.0	33.0	36.0	28.0	38.5
82-83	33.208520956263705	35.0	33.0	35.5	28.0	37.0
84-85	33.081065389028	35.0	33.0	35.0	28.0	37.0
86-87	32.93944611620992	35.0	33.0	35.0	28.0	36.5
88-89	32.83722610046554	35.0	33.0	35.0	28.0	36.0
90-91	32.77337246083431	35.0	33.0	35.0	28.0	36.0
92-93	32.704702834977745	35.0	33.0	35.0	27.0	36.0
94-95	32.658927545435574	35.0	33.0	35.0	27.0	35.5
96-97	32.57283974065412	35.0	33.0	35.0	27.0	35.0
98-99	32.49897371891778	35.0	33.0	35.0	27.0	35.0
100	31.240061408201374	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.40058425234477824
1101	2	0.4318453158453366
1101	3	0.3765265852747959
1101	4	0.25190875643332333
1101	5	0.2470869467723631
1101	6	0.2837899372639541
1101	7	0.3573155780685937
1101	8	0.31210153193775625
1101	9	0.4178842491579928
1101	10-11	0.44869653061731896
1101	12-13	0.417027082155748
1101	14-15	0.5867097868531346
1101	16-17	0.5908308138432048
1101	18-19	0.5421427847023423
1101	20-21	0.576306254208113
1101	22-23	0.5696779176031512
1101	24-25	0.5365919390404983
1101	26-27	0.6227124695244939
1101	28-29	0.6628862288070465
1101	30-31	0.6480571366598653
1101	32-33	0.6818243927296095
1101	34-35	0.6877136649315645
1101	36-37	0.5929907163171322
1101	38-39	0.6477119007594752
1101	40-41	0.6629471834797727
1101	42-43	0.6414506419682624
1101	44-45	0.5646531525625278
1101	46-47	0.6604500625808001
1101	48-49	0.5821436551992036
1101	50-51	0.5742282411621957
1101	52-53	0.5998255883147863
1101	54-55	0.5843561881947466
1101	56-57	0.596746895849364
1101	58-59	0.6094171143232003
1101	60-61	0.594128886306045
1101	62-63	0.5752670236676352
1101	64-65	0.6561516817321191
1101	66-67	0.5497525689393044
1101	68-69	0.5506576308634976
1101	70-71	0.5229812444529998
1101	72-73	0.5796376738453901
1101	74-75	0.5489872436597736
1101	76-77	0.48254451774318596
1101	78-79	0.5183599676920707
1101	80-81	0.5379848594873593
1101	82-83	0.4750635573595545
1101	84-85	0.4428778653988914
1101	86-87	0.4331033052416231
1101	88-89	0.4394029424133379
1101	90-91	0.483017598432113
1101	92-93	0.45970451023342207
1101	94-95	0.49045418558935694
1101	96-97	0.46405617747617356
1101	98-99	0.47381721561464474
1101	100	0.8576561569419638
1102	1	0.38050598432501914
1102	2	0.4245448710436861
1102	3	0.34336663646827503
1102	4	0.22654953079991458
1102	5	0.22686085935542621
1102	6	0.22900572157802657
1102	7	0.27760508439289566
1102	8	0.2972681063407805
1102	9	0.3860271341295274
1102	10-11	0.3768605315166482
1102	12-13	0.3709368281600618
1102	14-15	0.5280861589044079
1102	16-17	0.507465514219497
1102	18-19	0.5020619562182844
1102	20-21	0.5269160437956373
1102	22-23	0.5196995313989987
1102	24-25	0.5544494784422156
1102	26-27	0.5707137663522417
1102	28-29	0.568692140871697
1102	30-31	0.6288537729684336
1102	32-33	0.6068181792572176
1102	34-35	0.6062917300503301
1102	36-37	0.6143426770282261
1102	38-39	0.6106148627187977
1102	40-41	0.5612985092115395
1102	42-43	0.5730127109024465
1102	44-45	0.6131186915975633
1102	46-47	0.6558345688478155
1102	48-49	0.6459206719500941
1102	50-51	0.6060454279626839
1102	52-53	0.602139173276953
1102	54-55	0.5901168377307968
1102	56-57	0.5963900311378936
1102	58-59	0.5684470776824853
1102	60-61	0.5743711109701906
1102	62-63	0.5664470736994218
1102	64-65	0.5442384103477949
1102	66-67	0.5021309557756766
1102	68-69	0.5554387249957244
1102	70-71	0.48780704397575647
1102	72-73	0.5242997775117928
1102	74-75	0.5725258329782932
1102	76-77	0.4957168618838139
1102	78-79	0.4764689739276733
1102	80-81	0.5204154908591292
1102	82-83	0.514698892715181
1102	84-85	0.4796256252889819
1102	86-87	0.4926884902687547
1102	88-89	0.4607131770395867
1102	90-91	0.4408830444527396
1102	92-93	0.4800880978153259
1102	94-95	0.5022949104852472
1102	96-97	0.47915250506702733
1102	98-99	0.5000194780693477
1102	100	0.7726594108653444
1103	1	0.3260036677828495
1103	2	0.39258416585657585
1103	3	0.3024407036329464
1103	4	0.25616988364220816
1103	5	0.2395192829546957
1103	6	0.2337309373915204
1103	7	0.25779927071646114
1103	8	0.21842131428365974
1103	9	0.29905444366773537
1103	10-11	0.3277026181588596
1103	12-13	0.2980080518494006
1103	14-15	0.4353840931300539
1103	16-17	0.42256942314711665
1103	18-19	0.3653840455835251
1103	20-21	0.37538892768444043
1103	22-23	0.3859913063978837
1103	24-25	0.42765888757013926
1103	26-27	0.4832819321928383
1103	28-29	0.5225315689561185
1103	30-31	0.4136057101841928
1103	32-33	0.40283237175997755
1103	34-35	0.4884052049421328
1103	36-37	0.47106292396023974
1103	38-39	0.47246485303108443
1103	40-41	0.48278763704224303
1103	42-43	0.46413166982652854
1103	44-45	0.4602852067300347
1103	46-47	0.45967854623092563
1103	48-49	0.4519972831218553
1103	50-51	0.45850737586110313
1103	52-53	0.4714088161013734
1103	54-55	0.38496015857483457
1103	56-57	0.37520214706581356
1103	58-59	0.4149176564841994
1103	60-61	0.41457433534291255
1103	62-63	0.4794765924294424
1103	64-65	0.4634319015123438
1103	66-67	0.4603546068267974
1103	68-69	0.4155673048683184
1103	70-71	0.42335984595100484
1103	72-73	0.46135855281440286
1103	74-75	0.3762814426890557
1103	76-77	0.3571787299240512
1103	78-79	0.3769496695187158
1103	80-81	0.3662355854059953
1103	82-83	0.35536130169646896
1103	84-85	0.344062988319088
1103	86-87	0.38098213833500694
1103	88-89	0.3839638489611801
1103	90-91	0.32641982569628425
1103	92-93	0.3056366892346247
1103	94-95	0.3035185000062768
1103	96-97	0.35839983996348934
1103	98-99	0.34674464577155106
1103	100	0.5980646911937839
1104	1	0.2574945388098442
1104	2	0.2587506068282579
1104	3	0.2557117892517624
1104	4	0.19934727990034418
1104	5	0.17360305211283134
1104	6	0.1886366902788552
1104	7	0.25732712718482986
1104	8	0.24302239191235486
1104	9	0.2739027481017331
1104	10-11	0.3173357276131412
1104	12-13	0.33678640640325597
1104	14-15	0.3817434319734616
1104	16-17	0.34582680492963647
1104	18-19	0.3688020599346231
1104	20-21	0.40799358400546026
1104	22-23	0.3901034301098676
1104	24-25	0.4256094733394278
1104	26-27	0.4305100021634729
1104	28-29	0.43888401372570485
1104	30-31	0.411450744635097
1104	32-33	0.4034128229424141
1104	34-35	0.45851569820241167
1104	36-37	0.41691007138316394
1104	38-39	0.437163368632568
1104	40-41	0.4863295242155061
1104	42-43	0.4748588292346412
1104	44-45	0.44153387720497506
1104	46-47	0.4769231347396712
1104	48-49	0.45621528242661213
1104	50-51	0.41418927953344564
1104	52-53	0.39191667521581763
1104	54-55	0.4092729562587394
1104	56-57	0.4353634800670818
1104	58-59	0.40108248905483634
1104	60-61	0.37736238829562296
1104	62-63	0.34316136139040765
1104	64-65	0.34976594046677434
1104	66-67	0.32894548537695556
1104	68-69	0.342831421181657
1104	70-71	0.30471756990787213
1104	72-73	0.28133097003363616
1104	74-75	0.2988729269837975
1104	76-77	0.2423983998905257
1104	78-79	0.23570433258463908
1104	80-81	0.23878828648206962
1104	82-83	0.22941620394720275
1104	84-85	0.22845235367516636
1104	86-87	0.2521805073644714
1104	88-89	0.2724132919874691
1104	90-91	0.2702169103396841
1104	92-93	0.2812160703115296
1104	94-95	0.2527512343399323
1104	96-97	0.2319333089732325
1104	98-99	0.2694527067143895
1104	100	0.49613162661907495
1105	1	0.14933912297806273
1105	2	0.157332633564657
1105	3	0.15289922176000914
1105	4	0.18504315602100974
1105	5	0.15333960992792584
1105	6	0.15931311139177495
1105	7	0.17713122346995647
1105	8	0.1625350650443238
1105	9	0.20120421424537227
1105	10-11	0.18489259559590465
1105	12-13	0.16832041225323024
1105	14-15	0.26841171083755455
1105	16-17	0.2445002510820231
1105	18-19	0.27118741683645453
1105	20-21	0.25803439954269436
1105	22-23	0.2632604027333656
1105	24-25	0.25935541622938985
1105	26-27	0.31388126124620896
1105	28-29	0.3188162351642845
1105	30-31	0.30040282499988535
1105	32-33	0.3086253918247408
1105	34-35	0.2382954507409707
1105	36-37	0.2989547983460099
1105	38-39	0.2945028539548389
1105	40-41	0.3178947636746301
1105	42-43	0.297913317596624
1105	44-45	0.30537530368247445
1105	46-47	0.3129746068584396
1105	48-49	0.24879769135375795
1105	50-51	0.2841140081649911
1105	52-53	0.20133370071591372
1105	54-55	0.16128662777980907
1105	56-57	0.18221217186230376
1105	58-59	0.18537302658580046
1105	60-61	0.2675663660342593
1105	62-63	0.2864952563855141
1105	64-65	0.2612061379370587
1105	66-67	0.20803833751932643
1105	68-69	0.15553473839736398
1105	70-71	0.2115244923185955
1105	72-73	0.11790684087011272
1105	74-75	0.13685045356006498
1105	76-77	0.1427781516903437
1105	78-79	0.09700584196644968
1105	80-81	0.06907088383206883
1105	82-83	0.0874999182762437
1105	84-85	0.10272126994389197
1105	86-87	0.14075726838597546
1105	88-89	0.14137379836520836
1105	90-91	0.12653964662546713
1105	92-93	0.1419058355086591
1105	94-95	0.11510577806416222
1105	96-97	0.135591041632658
1105	98-99	0.15917144996521415
1105	100	0.3242194518342778
1106	1	0.17104552510517124
1106	2	0.2159154291993488
1106	3	0.1353038909457993
1106	4	0.14320328476421906
1106	5	0.11731206560495622
1106	6	0.14588648721473874
1106	7	0.1163258517478738
1106	8	0.06526362618153314
1106	9	0.08252496006922172
1106	10-11	0.17744371709535045
1106	12-13	0.16279781169927077
1106	14-15	0.20943997158055083
1106	16-17	0.18146427329998716
1106	18-19	0.22857264014893985
1106	20-21	0.18648098147109238
1106	22-23	0.23598380873280433
1106	24-25	0.20265325586336047
1106	26-27	0.20713347389599335
1106	28-29	0.20995681351377016
1106	30-31	0.23669371580423615
1106	32-33	0.294756197148331
1106	34-35	0.19951072902189537
1106	36-37	0.1545340285159469
1106	38-39	0.28422683659523784
1106	40-41	0.23558606050787034
1106	42-43	0.24196518996389216
1106	44-45	0.17073941507237578
1106	46-47	0.18837657629945426
1106	48-49	0.23471598358418078
1106	50-51	0.2845520405528248
1106	52-53	0.2090287358021854
1106	54-55	0.20802843110030977
1106	56-57	0.18405769888771317
1106	58-59	0.14089039850289709
1106	60-61	0.16756964733738755
1106	62-63	0.20679845873334557
1106	64-65	0.20982149341033818
1106	66-67	0.21351842760777373
1106	68-69	0.18642123010548772
1106	70-71	0.23040575654073336
1106	72-73	0.10809948488165588
1106	74-75	0.1716408720607845
1106	76-77	0.21237151100242357
1106	78-79	0.1612384164420888
1106	80-81	0.10327866504811567
1106	82-83	0.14948228257323848
1106	84-85	0.10536662277905151
1106	86-87	0.001231316116232506
1106	88-89	0.0523907297846975
1106	90-91	0.11992347235653966
1106	92-93	0.12775854745011372
1106	94-95	0.14545631487698074
1106	96-97	0.1140626655666992
1106	98-99	0.12408237272100564
1106	100	0.26318563131614425
1107	1	0.12619712287358453
1107	2	0.14761038259440795
1107	3	0.15010819438253264
1107	4	0.10930605955062589
1107	5	0.05126786086196944
1107	6	0.09769616982563889
1107	7	0.09427486535066265
1107	8	0.12353614858136552
1107	9	0.11589485370860331
1107	10-11	0.15699863038414463
1107	12-13	0.04411018765182462
1107	14-15	0.16877284921390157
1107	16-17	0.14985861849409332
1107	18-19	0.10624985989265667
1107	20-21	0.14357420558712874
1107	22-23	0.08899546954958026
1107	24-25	0.12722042962406732
1107	26-27	0.1875087675967535
1107	28-29	0.20419512789182903
1107	30-31	0.1407017369029333
1107	32-33	0.2171375500108752
1107	34-35	0.19368796432849678
1107	36-37	0.10500066993086676
1107	38-39	0.14500527830865906
1107	40-41	0.19264558958175826
1107	42-43	0.20381060033971465
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2216	88-89	-1.0268316451017157
2216	90-91	-0.9896553175149378
2216	92-93	-1.053831703659398
2216	94-95	-1.0509875556469268
2216	96-97	-1.065097878035811
2216	98-99	-1.021131873805917
2216	100	-0.3906399058968901
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	19.0
13	85.0
14	299.0
15	935.0
16	2193.0
17	4780.0
18	8387.0
19	13173.0
20	18611.0
21	26094.0
22	35953.0
23	41781.0
24	42854.0
25	42035.0
26	44122.0
27	53723.0
28	67154.0
29	85032.0
30	106356.0
31	133443.0
32	167567.0
33	215154.0
34	305880.0
35	440131.0
36	707352.0
37	1306746.0
38	1131883.0
39	452082.0
40	816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.421165003191774	22.14999836836341	12.889764065349063	25.539072563095754
2	14.277403637073006	21.05942200211357	39.584832157375246	25.07834220343818
3	17.05494728713834	27.48552335018625	29.756853148673628	25.70267621400179
4	11.445915774547407	17.331645976545815	39.694371171520295	31.528067077386485
5	12.08277965515016	39.23268270646696	35.670551947480504	13.013985690902372
6	29.215880855997355	39.73211445735313	17.683522093013238	13.368482593636283
7	25.632621603283663	32.673522355175216	24.355856102773362	17.337999938767762
8	26.53752415830468	34.73299642176596	19.71759487233452	19.01188454759484
9	26.38052038574089	13.927301606740382	21.678162065879253	38.01401594163948
10-11	25.502452404794006	26.05385390729297	27.690962896583937	20.75273079132908
12-13	25.74029796349082	23.894278399920214	28.30105921063728	22.064364425951688
14-15	22.983745877431463	24.815277393766625	26.030587130032245	26.170389598769667
16-17	21.2483633218361	27.122795869796136	27.54232813641802	24.086512671949748
18-19	20.59151285955509	27.454192444029996	30.218233447282184	21.736061249132735
20-21	22.24971153031746	26.655781145100892	29.34014416334259	21.75436316123906
22-23	22.712610497546496	26.295574451577608	28.945095612070887	22.046719438805013
24-25	22.135368638038106	26.290560377337048	29.99999450010138	21.57407648452347
26-27	22.14987920389334	26.496045756223225	28.80191491803501	22.552160121848424
28-29	21.287614264977048	27.185008889669465	29.263173082176188	22.2642037631773
30-31	22.307671295259123	26.775092279132323	29.183919543083086	21.733316882525465
32-33	22.899781214032956	26.935945980729088	28.30075498941636	21.863517815821595
34-35	22.569530634985295	26.71378674253105	29.243483445121825	21.47319917736183
36-37	22.23026022220324	26.248238657467414	28.923389345523066	22.598111774806284
38-39	22.456562717360576	26.26343671064997	29.740518449776577	21.53948212221288
40-41	21.924988349381426	25.543160821068867	30.149069252156785	22.382781577392922
42-43	22.141986849375744	25.766383372999822	29.135382937774512	22.95624683984992
44-45	22.26798036022869	26.928906110497365	28.846271600393496	21.956841928880444
46-47	22.39454219393889	26.64200389906146	28.059181109094887	22.90427279790476
48-49	23.399621166983156	26.483359323409804	27.920253669990686	22.196765839616354
50-51	22.61446480669873	26.367659789471215	27.747831848299597	23.27004355553046
52-53	21.847476444850866	27.08551572366016	27.58278905725505	23.484218774233927
54-55	22.012152575987514	27.054367964483856	27.35193081274202	23.581548646786608
56-57	23.33592317448282	26.339271146101872	27.09310558375374	23.23170009566157
58-59	21.985710155550052	26.8598329899867	28.19573159966656	22.958725254796693
60-61	22.199754117865762	27.57214675342818	27.203946873912628	23.024152254793435
62-63	22.739724997735873	26.66898090178538	26.687643891097608	23.90365020938114
64-65	21.72493630196079	26.970440956528403	27.279333822458007	24.0252889190528
66-67	22.44750958289903	26.43117412822133	27.268324014506184	23.852992274373452
68-69	21.91612434610789	26.355523346519643	27.735502908896382	23.99284939847609
70-71	21.924322867587478	26.060944667089547	27.758221470973233	24.256510994349746
72-73	22.590559216620253	25.972113738780962	28.26616842250191	23.171158622096875
74-75	22.705283454537874	26.508296403646632	27.595933540772073	23.19048660104343
76-77	22.426339055528437	26.46688253802384	27.144287773477156	23.962490632970567
78-79	22.05975716467604	26.543185319369204	27.376576248894747	24.020481267060006
80-81	21.63716032167807	27.069987676560498	28.02597088793663	23.2668811138248
82-83	22.581327917522053	25.80529515572596	29.129580544731958	22.483796382020024
84-85	23.005150105066395	26.493515802858703	27.39387670620605	23.107457385868855
86-87	21.33609587129944	27.187263848103065	28.011955312957067	23.46468496764043
88-89	22.482247243909136	25.91575145298155	27.72034152170462	23.881659781404696
90-91	21.99731201621517	25.86220077343241	28.066798468681558	24.073688741670864
92-93	22.552792610189552	26.181332390772933	27.603908667950222	23.661966331087296
94-95	21.12965717665271	26.27389568452288	28.34357170016166	24.25287543866275
96-97	21.54643032746763	26.17212922708458	27.8124098245789	24.46903062086889
98-99	20.89502233508829	26.912754741554263	27.76798897673653	24.42423394662092
100	21.747177969311934	26.14711523204077	26.790795965369764	25.314910833277537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	182.0
1	127.0
2	111.5
3	149.0
4	102.0
5	78.5
6	170.0
7	382.5
8	635.5
9	717.0
10	595.5
11	523.0
12	609.5
13	762.0
14	1005.5
15	1463.0
16	1942.5
17	2570.5
18	3594.5
19	4816.5
20	6165.0
21	7669.5
22	9344.0
23	10987.5
24	12833.0
25	14903.0
26	17790.5
27	20780.0
28	25163.5
29	35993.5
30	52230.0
31	66280.5
32	84940.5
33	103713.0
34	116629.5
35	133477.0
36	147808.0
37	171987.5
38	182049.0
39	167257.0
40	153346.0
41	140365.5
42	138444.5
43	147879.0
44	162977.5
45	197565.5
46	229771.0
47	250697.0
48	266840.0
49	259435.5
50	241261.5
51	243961.0
52	247436.5
53	233972.0
54	223409.5
55	189745.0
56	159819.0
57	130933.5
58	98445.5
59	97647.5
60	80992.5
61	49985.0
62	34623.0
63	29544.0
64	21222.0
65	8047.5
66	3069.0
67	1770.0
68	1227.5
69	786.0
70	455.0
71	282.5
72	152.5
73	61.5
74	13.5
75	3.5
76	3.0
77	2.0
78	2.0
79	0.5
80	0.0
81	0.5
82	0.5
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00678320829619374
3	0.0
4	0.08620174434784586
5	0.003923261014555299
6	5.499898618535465E-5
7	5.499898618535465E-5
8	0.0
9	0.0
10-11	0.0
12-13	3.299939171121279E-4
14-15	0.00956982359625171
16-17	0.0
18-19	1.0083147467315019E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	5.499898618535465E-5
60-61	0.0
62-63	0.0
64-65	7.333198158047286E-5
66-67	1.3749746546338663E-4
68-69	0.0
70-71	4.7665788027307363E-4
72-73	4.491583871803963E-4
74-75	4.308253917852781E-4
76-77	1.5583046085850485E-4
78-79	5.499898618535465E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.061250537615089966
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5454646.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.32209621911737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.70038805532076	44.685184833317535
2	16.386659986694944	20.425020008015263
3	5.908155987830802	11.046259978534371
4	2.5940593134014858	6.466688565116198
5	1.315795032900506	4.100155232253102
6	0.7355547461328223	2.750478819775092
7	0.4208951690009586	1.8361748454447588
8	0.27843322642894786	1.3882033862483347
9	0.17638121889947908	0.9893202565948686
>10	0.4743392945169403	4.134930000727553
>50	0.004061564636170121	0.16364080778682064
>100	0.0040139618756901314	0.515506223564737
>500	6.965199572253302E-4	0.2994378287973932
>1k	4.353249256084474E-4	0.41362949753272943
>5k	4.3532492560843745E-5	0.17908751890420477
>10k+	8.706498512168749E-5	0.6062821973871025
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12161	0.22294755700003263	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10186	0.18673989109467415	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6601	0.12101610260317536	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.8332995395118215E-5
7	0.0	0.0	0.0	0.0	1.8332995395118215E-5
8	0.0	0.0	0.0	0.0	5.499898618535465E-5
9	0.0	0.0	0.0	0.0	5.499898618535465E-5
10-11	0.0	0.0	0.0	0.0	5.499898618535465E-5
12-13	0.0	0.0	0.0	0.0	6.416548388291375E-5
14-15	0.0	0.0	0.0	0.0	1.099979723707093E-4
16-17	0.0	0.0	0.0	0.0	1.099979723707093E-4
18-19	0.0	0.0	0.0	0.0	1.3749746546338663E-4
20-21	0.0	0.0	0.0	2.749949309267732E-5	1.4666396316094572E-4
22-23	0.0	0.0	0.0	1.4666396316094572E-4	1.8332995395118216E-4
24-25	0.0	0.0	0.0	3.116609217170097E-4	2.108294470438595E-4
26-27	0.0	0.0	0.0	7.883188019900834E-4	2.199959447414186E-4
28-29	0.0	0.0	0.0	0.0035382681112578157	2.199959447414186E-4
30-31	0.0	0.0	0.0	0.013309754656855825	2.199959447414186E-4
32-33	0.0	0.0	0.0	0.030414439360501123	2.199959447414186E-4
34-35	0.0	0.0	0.0	0.05641979332847631	2.9332792632189144E-4
36-37	0.0	0.0	0.0	0.10272893969654492	2.9332792632189144E-4
38-39	0.0	0.0	0.0	0.1733659709539354	3.208274194145688E-4
40-41	0.0	0.0	0.0	0.24780709875581292	3.299939171121279E-4
42-43	0.0	0.0	0.0	0.3271339698304895	3.299939171121279E-4
44-45	0.0	0.0	0.0	0.42307970123084065	3.39160414809687E-4
46-47	0.0	0.0	0.0	0.5430599895941919	3.4832691250724613E-4
48-49	0.0	0.0	0.0	0.6814282723388465	4.0332589869260076E-4
50-51	0.0	0.0	0.0	0.8309980152699186	4.0332589869260076E-4
52-53	0.0	0.0	0.0	0.978771124652269	5.224903687608692E-4
54-55	0.0	0.0	0.0	1.136838210948978	6.416548388291376E-4
56-57	0.0	0.0	0.0	1.3336612495109672	6.416548388291376E-4
58-59	0.0	0.0	0.0	1.571165204854724	6.416548388291376E-4
60-61	0.0	0.0	0.0	1.8154340354992788	6.416548388291376E-4
62-63	0.0	0.0	0.0	2.076734218865899	6.78320829619374E-4
64-65	0.0	0.0	0.0	2.3484383771192485	6.78320829619374E-4
66-67	0.0	0.0	0.0	2.656047706853937	6.78320829619374E-4
68-69	0.0	0.0	0.0	3.0055112650756803	6.78320829619374E-4
70-71	0.0	0.0	0.0	3.3886708688336515	6.966538250144923E-4
72-73	0.0	0.0	0.0	3.789457647664028	7.883188019900833E-4
74-75	0.0	0.0	0.0	4.181682917644885	7.883188019900833E-4
76-77	0.0	0.0	0.0	4.6279538580505495	7.974852996876424E-4
78-79	0.0	0.0	0.0	5.110377465375388	8.066517973852015E-4
80-81	0.0	0.0	0.0	5.642043498331514	8.158182950827606E-4
82-83	0.0	0.0	0.0	6.196974102444045	8.524842858729971E-4
84-85	0.0	0.0	0.0	6.73311338627658	8.616507835705561E-4
86-87	0.0	0.0	0.0	7.300611258732465	8.708172812681152E-4
88	0.0	0.0	0.0	7.76250191121477	8.799837789656744E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6175	0.0	44.550354	94
GGTATCA	10655	0.0	39.68716	1
GTATCAA	18475	0.0	39.259407	1
CTGTGCG	8265	0.0	36.001274	9
CAACGCA	20450	0.0	33.553432	5
ACTGTGC	9005	0.0	33.50678	8
ATCAACG	20855	0.0	32.94974	3
TAGTACT	9075	0.0	32.910843	4
GTGGTAT	2500	0.0	32.36177	1
GTACTAG	6460	0.0	31.965082	1
GTACTGT	9385	0.0	31.899109	6
TACTGTG	10570	0.0	31.035435	7
TATCAAC	23500	0.0	30.80453	2
AACGCAG	23995	0.0	29.790781	6
CTAGTAC	10380	0.0	29.543518	3
CAGTACG	860	0.0	28.969065	9
TATTCTC	3315	0.0	28.921715	5
CTAACGC	3115	0.0	28.83947	3
TAACGCC	3210	0.0	28.425533	4
TCTAACG	3235	0.0	27.771727	2
>>END_MODULE