FastQCFastQC Report
Mon 16 Jan 2017
ERR1146652_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146652_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5149485
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC61000.042.63739894
GTACTAG62400.036.3920331
GCATCGT60800.034.5474328
TAGTACT85300.033.2761764
CTGTGCG82400.033.1900259
TAGGCAT74100.032.7232135
CAACGCA86200.032.1639375
TCTAACG31900.031.9682482
CTAACGC31800.031.7729033
CAGTACG6850.031.5557929
ACTGTGC89900.030.9438158
TAACGCC33450.030.4866124
GTATCAA102000.030.4219881
CGCCTAC33100.030.238727
ATCAACG92300.030.1415463
GTACTGT92700.030.0091576
CTAGGCA85350.029.8429184
TACTGTG101900.029.3749437
CTAGTAC103000.028.5159823
ACGCCTA36750.027.6190176
Produced by FastQC (version 0.11.5)