##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146652_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5149485
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18961430123595	31.0	31.0	34.0	28.0	34.0
2	31.28595131357796	31.0	31.0	34.0	28.0	34.0
3	31.378240930889206	31.0	31.0	34.0	28.0	34.0
4	34.83650170842327	37.0	35.0	37.0	32.0	37.0
5	34.99017416304737	37.0	35.0	37.0	32.0	37.0
6	35.12435146427264	37.0	35.0	37.0	32.0	37.0
7	35.08961828221657	37.0	35.0	37.0	32.0	37.0
8	35.0844165969995	37.0	35.0	37.0	32.0	37.0
9	36.59464257105322	39.0	37.0	39.0	32.0	39.0
10-11	36.424192613436105	39.0	37.0	39.0	31.5	39.0
12-13	36.34013566405184	39.0	37.0	39.0	31.0	39.0
14-15	37.494737434908544	40.0	37.0	41.0	31.0	41.0
16-17	37.512613105970786	40.0	37.0	41.0	31.0	41.0
18-19	37.469331302062244	40.0	37.0	41.0	31.0	41.0
20-21	37.53337586185803	40.0	37.0	41.0	31.0	41.0
22-23	37.47029304872235	40.0	37.0	41.0	31.0	41.0
24-25	37.413708264030284	40.0	37.0	41.0	31.0	41.0
26-27	37.44623035119046	40.0	37.0	41.0	31.0	41.0
28-29	37.42675325785005	40.0	37.0	41.0	31.0	41.0
30-31	37.2884645746128	40.0	36.0	41.0	30.0	41.0
32-33	37.28016209387929	40.0	36.0	41.0	30.0	41.0
34-35	37.21522307570562	40.0	36.0	41.0	30.0	41.0
36-37	37.0956078132085	40.0	36.0	41.0	30.0	41.0
38-39	36.985852662936196	40.0	36.0	41.0	30.0	41.0
40-41	36.89768103023894	40.0	35.5	41.0	30.0	41.0
42-43	36.73476172860005	39.0	35.0	41.0	30.0	41.0
44-45	36.57341365204482	39.0	35.0	41.0	29.0	41.0
46-47	36.573063034458784	39.0	35.0	41.0	29.5	41.0
48-49	36.42561828998434	39.0	35.0	41.0	29.0	41.0
50-51	36.24186438061282	39.0	35.0	41.0	29.0	41.0
52-53	36.01964147871098	38.0	35.0	40.0	28.0	41.0
54-55	35.79950810615043	38.0	34.0	40.0	28.0	41.0
56-57	35.546429594415756	37.5	34.0	40.0	28.0	41.0
58-59	35.22990172803688	37.0	34.0	40.0	27.0	41.0
60-61	34.97943639024096	36.5	33.5	40.0	27.0	41.0
62-63	34.667213323274076	36.0	33.0	39.5	26.5	41.0
64-65	34.51130384883149	35.5	33.0	39.0	26.0	41.0
66-67	34.25258953079774	35.0	33.0	39.0	26.0	41.0
68-69	34.12641846708942	35.0	33.0	39.0	26.0	41.0
70-71	34.05865033105252	35.0	33.0	38.0	26.0	41.0
72-73	33.83917148996453	35.0	33.0	37.0	26.0	40.0
74-75	33.59132816194241	35.0	33.0	37.0	26.0	39.5
76-77	33.33084017139578	35.0	33.0	36.5	26.0	39.0
78-79	33.08080672144884	35.0	33.0	36.0	26.0	39.0
80-81	32.902350623411856	35.0	33.0	36.0	26.0	38.5
82-83	32.64063056791116	35.0	32.5	35.0	25.0	37.0
84-85	32.46530420032295	35.0	32.0	35.0	25.0	37.0
86-87	32.29445973723586	35.0	32.0	35.0	25.0	36.5
88-89	32.174254707024105	35.0	32.0	35.0	25.0	36.0
90-91	32.0243868076128	35.0	32.0	35.0	25.0	36.0
92-93	31.862816572919428	35.0	32.0	35.0	24.0	36.0
94-95	31.79175150524761	35.0	32.0	35.0	24.0	35.5
96-97	31.683332313813906	35.0	32.0	35.0	24.0	35.0
98-99	31.59159964540143	35.0	31.5	35.0	24.0	35.0
100	30.606180229673452	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3982489966675864
1101	2	0.47182109273639483
1101	3	0.49056939698322566
1101	4	0.6172072134357194
1101	5	0.5590066953764321
1101	6	0.5120577730088343
1101	7	0.5455333913259395
1101	8	0.543693449908794
1101	9	0.731231532821063
1101	10-11	0.7311490221290384
1101	12-13	0.6354908376443262
1101	14-15	0.8538793876630066
1101	16-17	0.7986100973354269
1101	18-19	0.8508588993985171
1101	20-21	0.8219329667175117
1101	22-23	0.8404374637546752
1101	24-25	0.8654745153328136
1101	26-27	0.8011726852246994
1101	28-29	0.7747968063413282
1101	30-31	0.8566377412954296
1101	32-33	0.8201360383831826
1101	34-35	0.8342563463012027
1101	36-37	0.866954408152246
1101	38-39	0.8135937914337603
1101	40-41	0.8055536516169255
1101	42-43	0.7573513975577413
1101	44-45	0.8312582363348113
1101	46-47	0.8376564338953187
1101	48-49	0.761836415167835
1101	50-51	0.7182704656565448
1101	52-53	0.7364738024940394
1101	54-55	0.7350916541898727
1101	56-57	0.72771811165169
1101	58-59	0.709682431587126
1101	60-61	0.7240240302240935
1101	62-63	0.7513928019794704
1101	64-65	0.7657439925268505
1101	66-67	0.7581764486762594
1101	68-69	0.7612923854660139
1101	70-71	0.7339860243150014
1101	72-73	0.7499073745606353
1101	74-75	0.673242348879505
1101	76-77	0.6838052056293478
1101	78-79	0.7117144542882841
1101	80-81	0.6841865288638473
1101	82-83	0.652188537895924
1101	84-85	0.6101919915374481
1101	86-87	0.6318798903251519
1101	88-89	0.6713681304535584
1101	90-91	0.6252856819493644
1101	92-93	0.6577127864837848
1101	94-95	0.6502505797685387
1101	96-97	0.5829659214464051
1101	98-99	0.6479275135442393
1101	100	0.8253718744132534
1102	1	0.33224418897528096
1102	2	0.47125378504408744
1102	3	0.4729011277524542
1102	4	0.5414620211280265
1102	5	0.3757230415302786
1102	6	0.45090392685499125
1102	7	0.45720646824901223
1102	8	0.47282806529341315
1102	9	0.6397363405133731
1102	10-11	0.6945769067444303
1102	12-13	0.6044836261058606
1102	14-15	0.7699587145860889
1102	16-17	0.7571485588738867
1102	18-19	0.8238588993985161
1102	20-21	0.7892671013328965
1102	22-23	0.8094566945239023
1102	24-25	0.7705682653328196
1102	26-27	0.7840188390708605
1102	28-29	0.7682319024951738
1102	30-31	0.8100295682185035
1102	32-33	0.7294677691524143
1102	34-35	0.787862115531965
1102	36-37	0.8568246004599374
1102	38-39	0.8056274452799173
1102	40-41	0.73173153623231
1102	42-43	0.770901878326967
1102	44-45	0.7775178517194234
1102	46-47	0.7162357608183925
1102	48-49	0.706050357475533
1102	50-51	0.6622704656565475
1102	52-53	0.677800725570961
1102	54-55	0.6514185772667958
1102	56-57	0.6547926308824543
1102	58-59	0.678881950817896
1102	60-61	0.6707716263779346
1102	62-63	0.6686307827486999
1102	64-65	0.684138223296074
1102	66-67	0.6713831794454919
1102	68-69	0.5893981546967808
1102	70-71	0.6036278512380733
1102	72-73	0.6214746822529378
1102	74-75	0.6058505219564339
1102	76-77	0.5745792440908915
1102	78-79	0.5847048389036686
1102	80-81	0.5807562404023088
1102	82-83	0.540722191742077
1102	84-85	0.5461126646143697
1102	86-87	0.5268462364790025
1102	88-89	0.5313681304535578
1102	90-91	0.5709996242570554
1102	92-93	0.5823257672530104
1102	94-95	0.6961087528454613
1102	96-97	0.596727940677173
1102	98-99	0.5214515520057788
1102	100	0.7140209128747941
1103	1	0.284512141182482
1103	2	0.3199112949136911
1103	3	0.3431070809167096
1103	4	0.4238483337850454
1103	5	0.26831728874884675
1103	6	0.3142742462711894
1103	7	0.3372261326825594
1103	8	0.3614129737752876
1103	9	0.41439258677488766
1103	10-11	0.5724075010140695
1103	12-13	0.5439636214701409
1103	14-15	0.6875644576474542
1103	16-17	0.6037861650468699
1103	18-19	0.6558999629228595
1103	20-21	0.5606313307562729
1103	22-23	0.659330894488015
1103	24-25	0.6399843699805032
1103	26-27	0.6404830542879907
1103	28-29	0.5861587814579678
1103	30-31	0.5257215805704618
1103	32-33	0.5941433807686352
1103	34-35	0.5974720793434329
1103	36-37	0.5939335548803228
1103	38-39	0.6555121130033328
1103	40-41	0.5617926978545142
1103	42-43	0.5579927945549912
1103	44-45	0.448318784845398
1103	46-47	0.4425901206401477
1103	48-49	0.454265884901055
1103	50-51	0.433820591269658
1103	52-53	0.420368990315545
1103	54-55	0.3414168201786296
1103	56-57	0.3887923804039346
1103	58-59	0.3691438504171245
1103	60-61	0.4657378491617621
1103	62-63	0.3833236848601942
1103	64-65	0.4060253733738364
1103	66-67	0.43317249335864716
1103	68-69	0.4120806675571771
1103	70-71	0.40687575394773035
1103	72-73	0.40773144891163327
1103	74-75	0.39054151744034016
1103	76-77	0.3409588573819491
1103	78-79	0.4095738049881277
1103	80-81	0.3334304725173922
1103	82-83	0.4712775811427292
1103	84-85	0.3595671934156641
1103	86-87	0.3437602662672532
1103	88-89	0.45957369481532595
1103	90-91	0.5154428329243643
1103	92-93	0.39610161887999595
1103	94-95	0.3654042913369082
1103	96-97	0.43798782897123445
1103	98-99	0.39717644433739707
1103	100	0.478207530592222
1104	1	0.24630910733157307
1104	2	0.40879392975852724
1104	3	0.3286478678081757
1104	4	0.4835339236972871
1104	5	0.34796393883719645
1104	6	0.3258304088237267
1104	7	0.34369511164786815
1104	8	0.37740069336955884
1104	9	0.4469050740685532
1104	10-11	0.5457795553282381
1104	12-13	0.466070817523601
1104	14-15	0.6026228484275933
1104	16-17	0.6151649263092622
1104	18-19	0.610327712275776
1104	20-21	0.5290559546450808
1104	22-23	0.5418696820645295
1104	24-25	0.6104591732805105
1104	26-27	0.5435718300939243
1104	28-29	0.5040307097618495
1104	30-31	0.4840858046757148
1104	32-33	0.5129119438157801
1104	34-35	0.5643336853353986
1104	36-37	0.5562045339067723
1104	38-39	0.4847698477717941
1104	40-41	0.5575789282768824
1104	42-43	0.6169450846804807
1104	44-45	0.5845804194334008
1104	46-47	0.5431560566317302
1104	48-49	0.4895050519887576
1104	50-51	0.41530114976117716
1104	52-53	0.42155604595480156
1104	54-55	0.5466106431234792
1104	56-57	0.528662653905208
1104	58-59	0.5552010432571493
1104	60-61	0.4915742062804256
1104	62-63	0.5317891802490848
1104	64-65	0.5213047319634683
1104	66-67	0.4923933752356149
1104	68-69	0.4974875564921746
1104	70-71	0.5084719398079613
1104	72-73	0.4417847638966492
1104	74-75	0.41735452191773703
1104	76-77	0.4209925798748131
1104	78-79	0.4146675478496462
1104	80-81	0.4569614282602288
1104	82-83	0.4527211083184639
1104	84-85	0.42003492413302723
1104	86-87	0.4504421388161006
1104	88-89	0.4307990912181481
1104	90-91	0.47886641635579963
1104	92-93	0.4580910561014875
1104	94-95	0.4964394630683415
1104	96-97	0.48810299388101797
1104	98-99	0.4698298022766423
1104	100	0.5474941078136553
1105	1	0.09939997675470735
1105	2	0.21119757064554534
1105	3	0.2147526577175114
1105	4	0.240936600492347
1105	5	0.13862041659609758
1105	6	0.18175752409782575
1105	7	0.20491585865637063
1105	8	0.21144547231078548
1105	9	0.305847120873338
1105	10-11	0.3406621677419821
1105	12-13	0.2022313479119049
1105	14-15	0.3572804455348191
1105	16-17	0.3301114974598818
1105	18-19	0.3261185447003214
1105	20-21	0.29367386590855205
1105	22-23	0.32632183681005245
1105	24-25	0.43109982647158773
1105	26-27	0.3344566771350941
1105	28-29	0.38544195257654934
1105	30-31	0.43406761839561625
1105	32-33	0.39793527609320734
1105	34-35	0.38209101493841047
1105	36-37	0.3963114009960549
1105	38-39	0.43662008265965113
1105	40-41	0.4319209120400771
1105	42-43	0.4222222360767205
1105	44-45	0.43159496626635274
1105	46-47	0.4767869177160975
1105	48-49	0.32068640738936693
1105	50-51	0.3713479392097483
1105	52-53	0.40906613292340666
1105	54-55	0.3243872749117145
1105	56-57	0.35487045297091413
1105	58-59	0.4065548646922892
1105	60-61	0.4001608146049236
1105	62-63	0.3733581100068548
1105	64-65	0.37222691878695713
1105	66-67	0.36917454295131336
1105	68-69	0.4016876872706163
1105	70-71	0.4060291170343504
1105	72-73	0.2732536331169513
1105	74-75	0.2280797788732869
1105	76-77	0.24228894862872608
1105	78-79	0.32294551371578706
1105	80-81	0.22418287298332018
1105	82-83	0.3274563116980431
1105	84-85	0.25479766204149
1105	86-87	0.18080712274581145
1105	88-89	0.256583010665139
1105	90-91	0.27687225631153467
1105	92-93	0.2929861218913743
1105	94-95	0.3169126052819138
1105	96-97	0.22722735579612774
1105	98-99	0.2343804149132538
1105	100	0.30828242201748424
1106	1	0.08708689930964297
1106	2	0.16097611746796758
1106	3	0.16890665117552928
1106	4	0.2912791008769062
1106	5	0.08786840969661824
1106	6	0.2008806105043135
1106	7	0.13613338830989363
1106	8	0.18050639477548458
1106	9	0.283465909706095
1106	10-11	0.33299524905629596
1106	12-13	0.2463490835123423
1106	14-15	0.3505760338205661
1106	16-17	0.3126334113660647
1106	18-19	0.3318476495493172
1106	20-21	0.30340279118367164
1106	22-23	0.334944438359571
1106	24-25	0.41982716643669704
1106	26-27	0.42964676231481747
1106	28-29	0.38688599080266073
1106	30-31	0.4838031035224759
1106	32-33	0.4361565022509595
1106	34-35	0.4448869636856827
1106	36-37	0.42987536514343816
1106	38-39	0.5066885857394681
1106	40-41	0.43840204255673143
1106	42-43	0.42879391927326793
1106	44-45	0.38634303245014223
1106	46-47	0.43456966981038647
1106	48-49	0.33395591842516126
1106	50-51	0.3755667016319393
1106	52-53	0.33704336758029996
1106	54-55	0.45035181072262986
1106	56-57	0.378650876460469
1106	58-59	0.49146527632111514
1106	60-61	0.4003381438083551
1106	62-63	0.44047806558183566
1106	64-65	0.38016448203100595
1106	66-67	0.37351636452860504
1106	68-69	0.3776860095461174
1106	70-71	0.34967582404959074
1106	72-73	0.26771133915225676
1106	74-75	0.291612025559445
1106	76-77	0.27504825364479046
1106	78-79	0.23390328134824756
1106	80-81	0.21214995931384806
1106	82-83	0.2641094194859903
1106	84-85	0.27392877371865154
1106	86-87	0.2582337855477448
1106	88-89	0.2998766583218213
1106	90-91	0.2720256234380827
1106	92-93	0.287565573309692
1106	94-95	0.2862393751600223
1106	96-97	0.2624021471672435
1106	98-99	0.27600975356113366
1106	100	0.18170927940782633
1107	1	0.07205731736029364
1107	2	0.18214772653743339
1107	3	0.09337087593658211
1107	4	0.21611925047275804
1107	5	0.14007296386334644
1107	6	0.2015846975442912
1107	7	0.21214843683219442
1107	8	0.2528272509453231
1107	9	0.2839908868850287
1107	10-11	0.3850956312808549
1107	12-13	0.24752420889954152
1107	14-15	0.37785631851877355
1107	16-17	0.2621306067526916
1107	18-19	0.20845392531180096
1107	20-21	0.22380019273192175
1107	22-23	0.34239602619936704
1107	24-25	0.3674206504611206
1107	26-27	0.3076830347380408
1107	28-29	0.30253574579271714
1107	30-31	0.4103052691048106
1107	32-33	0.3597430886928805
1107	34-35	0.30763775351898914
1107	36-37	0.3113622816827757
1107	38-39	0.32141672785140685
1107	40-41	0.22729327840241353
1107	42-43	0.3038847609125668
1107	44-45	0.31116938852795784
1107	46-47	0.29866625408239855
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2214	100	-0.35372553021463915
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2215	4	-2.370629224129864
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2215	26-27	-1.0908628604206285
2215	28-29	-0.9154621128611851
2215	30-31	-3.5663368783268226
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2216	3	-0.638019896730377
2216	4	-0.4228290901330425
2216	5	-0.4130648665169545
2216	6	-0.3998979534358398
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2216	12-13	-0.6964350993423807
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2216	16-17	-0.7947652573009805
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2216	54-55	-1.4199815222324048
2216	56-57	-1.3003148602013823
2216	58-59	-1.1953266769689392
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2216	62-63	-1.3498427139231737
2216	64-65	-1.3359142219721818
2216	66-67	-1.2924824937606516
2216	68-69	-1.3329090853418109
2216	70-71	-1.2026795348906134
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2216	74-75	-1.1806271075935442
2216	76-77	-1.2444321207854685
2216	78-79	-1.030663821432185
2216	80-81	-1.1529961088843983
2216	82-83	-1.1652685790967539
2216	84-85	-1.115515685407452
2216	86-87	-1.1395158185743526
2216	88-89	-1.1837722775649553
2216	90-91	-1.2579309262334064
2216	92-93	-1.2530783513391235
2216	94-95	-1.265739845048543
2216	96-97	-1.1730708300841286
2216	98-99	-1.2507914371053026
2216	100	-0.8626235339785637
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	2.0
2	13.0
3	36.0
4	33.0
5	41.0
6	47.0
7	206.0
8	507.0
9	843.0
10	1304.0
11	2748.0
12	4996.0
13	7604.0
14	10283.0
15	13564.0
16	16204.0
17	18762.0
18	21048.0
19	24216.0
20	27735.0
21	31333.0
22	36018.0
23	41816.0
24	48568.0
25	56134.0
26	62103.0
27	69391.0
28	79707.0
29	95072.0
30	110854.0
31	129861.0
32	160919.0
33	207855.0
34	303173.0
35	425258.0
36	690727.0
37	1150643.0
38	947852.0
39	351601.0
40	408.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49506470291066	20.661407531185638	11.445575719892744	24.39795204601096
2	15.971543565976262	19.920280656833942	39.92096427622574	24.18721150096406
3	18.597626166354797	26.776505591802767	28.864908221023082	25.760960020819358
4	11.879771325938224	16.31126349488482	40.792734416937854	31.016230762239104
5	12.741243533841143	39.1013046604894	35.45687392230686	12.700577883362602
6	29.60668311669043	39.03217386947516	18.1621157221497	13.199027291684711
7	26.80753255161681	31.957454766414273	23.990622664186397	17.244390017782525
8	26.625012671364424	34.783914882609764	19.818577132974173	18.77249531305164
9	26.506231160580118	14.466790770728169	21.357226666500953	37.66975140219076
10-11	25.84646151148331	25.62095205673847	27.829429988805604	20.703156442972613
12-13	25.700856583881066	23.67459813555443	27.83448537824258	22.79005990232192
14-15	23.16137378236409	24.984685336460903	24.962566583224525	26.89137429795048
16-17	22.023943148857896	27.507608582512145	26.41423896308886	24.0542093055411
18-19	21.24215709140313	28.55277035349964	28.01918360538137	22.18588894971586
20-21	23.063079158569106	27.556934561362713	26.931944574582587	22.448041705485593
22-23	23.678767147429223	27.254633455570314	26.454592441270368	22.612006955730095
24-25	23.13837583503312	27.115026416074116	27.556561450640622	22.190036298252146
26-27	23.071849203742136	27.35234877790801	26.30993635937895	23.265865658970906
28-29	22.105608020402123	28.027469147071	26.872741912054295	22.99418092047258
30-31	22.96186002648558	27.718835698709142	26.880873344432754	22.438430930372522
32-33	23.46694650475572	27.78402258384267	25.867042986362073	22.88198792503954
34-35	23.36442009808134	27.500673635578444	27.056914074201384	22.077992192138833
36-37	22.90639229300472	27.041078461960794	26.641139560770256	23.41138968426423
38-39	23.21144231021327	27.254995256484825	27.308460547256967	22.225101886044936
40-41	22.70068048216956	26.273148755045256	28.02157562918723	23.00459513359796
42-43	22.669208542520806	26.533724785581427	27.18173415339938	23.61533251849839
44-45	22.844358575230586	27.621902968651607	27.122300956530555	22.41143749958725
46-47	22.804032498192182	27.35321807192854	26.575997029020847	23.26675240085843
48-49	23.544642928248063	26.95826025914927	26.77448139747688	22.722615415125787
50-51	23.0271667063872	26.61262926410579	26.86579180498443	23.49441222452258
52-53	22.427945029807393	27.358652467480702	26.7158177946171	23.497584708094806
54-55	22.380536905321254	27.29193612578279	26.71591597517768	23.61161099371828
56-57	23.713173438173875	26.604672355995834	26.358267695507042	23.323886510323252
58-59	22.43461969240074	27.084035998876963	27.430424821592958	23.050919487129338
60-61	22.45814770484281	27.83804374479532	26.560495667522076	23.143312882839794
62-63	22.961308435664723	27.02655775271904	26.133423761547636	23.8787100500686
64-65	22.047027778336155	27.218832630700774	26.669292495480303	24.06484709548277
66-67	22.76191805227441	26.726834861427573	26.63376342939482	23.8774836569032
68-69	22.187518722654	26.8840658307207	26.90077724078792	24.02763820583738
70-71	22.110878680996755	26.545184977448926	27.092371128334374	24.25156521321994
72-73	22.84499848465854	26.41047410232405	27.719843709594738	23.02468370342267
74-75	23.025288668969125	26.767252283156573	27.086925763483965	23.12053328439034
76-77	22.723653678922215	26.650197105494044	26.763891458211646	23.862257757372095
78-79	22.505962779206207	26.665696216926012	26.79660011122947	24.031740892638318
80-81	21.979052494227673	27.111139941329427	27.58336532225165	23.32644224219125
82-83	22.87992043675112	25.934894392449493	28.736096386347082	22.449088784452307
84-85	23.176120621620814	26.489055077745327	27.234069371590348	23.100754929043507
86-87	21.595596706444248	27.302016483315867	27.845213134137275	23.25717367610261
88-89	23.022377954167382	25.897712350421738	27.411165836751216	23.668743858659667
90-91	22.50214202205911	25.951868184094547	27.609753927962995	23.936235865883347
92-93	22.926448706481466	26.199949368794726	27.559903153982273	23.313698770741535
94-95	21.61938899398347	26.26781500448965	28.239386350785402	23.87340965074148
96-97	21.948535795148434	26.089681017716664	27.80871511756405	24.153068069570853
98-99	21.24331259870329	26.8569570774028	27.683386986620295	24.216343337273617
100	22.208770707663454	26.021029013605933	26.708373717081805	25.061826561648804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	367.0
1	265.5
2	202.0
3	245.5
4	213.0
5	176.0
6	179.0
7	187.5
8	201.0
9	219.0
10	204.5
11	189.5
12	191.0
13	199.0
14	226.5
15	379.5
16	474.0
17	533.5
18	756.0
19	929.0
20	1180.0
21	1595.5
22	2309.5
23	3304.5
24	4434.5
25	5988.0
26	8871.5
27	12005.0
28	16568.5
29	27475.5
30	43462.0
31	58347.5
32	77125.0
33	94410.0
34	106958.0
35	122763.5
36	136284.0
37	159200.0
38	168289.5
39	157764.0
40	147811.0
41	135604.5
42	133543.5
43	142516.5
44	156554.5
45	186027.5
46	215561.5
47	235654.5
48	247378.5
49	241516.0
50	229127.0
51	236298.0
52	241659.0
53	230446.0
54	217104.5
55	184860.5
56	160753.5
57	131560.5
58	100383.0
59	98380.5
60	82395.0
61	54526.0
62	39864.0
63	33594.5
64	24385.5
65	10940.0
66	5456.0
67	3644.5
68	2493.0
69	1741.5
70	1159.0
71	745.0
72	440.5
73	232.0
74	136.5
75	94.5
76	72.5
77	42.5
78	29.0
79	29.5
80	28.5
81	25.0
82	18.5
83	16.5
84	11.5
85	11.5
86	16.0
87	14.5
88	9.5
89	12.0
90	11.0
91	5.0
92	4.0
93	4.5
94	6.0
95	6.5
96	3.5
97	2.5
98	3.0
99	4.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05602501997772593
2	0.008175574839037303
3	0.008350349598066603
4	0.0096514505819514
5	0.0034178175099063303
6	0.002932332068158272
7	9.32132048156272E-4
8	0.001534133995923864
9	8.350349598066603E-4
10-11	6.505504919423981E-4
12-13	3.5925922689356314E-4
14-15	1.6506505019433983E-4
16-17	7.185184537871263E-4
18-19	0.0017089087549531652
20-21	8.253252509716991E-4
22-23	0.0011942941867002235
24-25	0.001233133022040068
26-27	0.003485785471751059
28-29	0.004214013634373146
30-31	0.005204403935539185
32-33	0.00615595540136538
34-35	0.0036411408131104372
36-37	0.008709608824960165
38-39	0.006796796184472817
40-41	0.006641440843113438
42-43	0.015419017629918333
44-45	0.021108907007205573
46-47	0.019691289517301244
48-49	0.02287607401516851
50-51	0.019691289517301244
52-53	0.026206504145560187
54-55	0.020468066224098136
56-57	0.01632202055156972
58-59	0.01816686523021234
60-61	0.016943441917007234
62-63	0.012612911776614555
64-65	0.01232162051156572
66-67	0.014593692378946633
68-69	0.005990890351171039
70-71	0.0098262253409807
72-73	0.00933103019039768
74-75	0.012379878764575487
76-77	0.011506004969428982
78-79	0.013001300130013
80-81	0.011001100110011
82-83	0.007262862208550953
84-85	0.011467166134089136
86-87	0.010117516606029536
88-89	0.009059158343018767
90-91	0.014147045772538419
92-93	0.009447546696417215
94-95	0.008146445712532419
96-97	0.010622421465447516
98-99	0.010302001073893797
100	0.2591132899697737
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5149485.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.63229859577116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.49918799409703	47.504214649811985
2	15.295975661590095	19.77228132547079
3	5.427818595172267	10.524371765005595
4	2.4536277237987023	6.3433439874968025
5	1.2900729603187955	4.169019039082741
6	0.709107421092783	2.7498745545927594
7	0.4127271635530943	1.867285369134449
8	0.2660783635048587	1.3757804991936136
9	0.17482263275478688	1.0169269741355609
>10	0.46657472760357366	4.265113768548674
>50	0.0030127297609785484	0.12782655811702898
>100	8.175924851125829E-4	0.07992702868231524
>500	8.821713395192171E-5	0.0422209118377253
>1k	8.821713392795277E-5	0.16181356888994014
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.941941766992233E-5
7	0.0	0.0	0.0	0.0	1.941941766992233E-5
8	0.0	0.0	0.0	0.0	1.941941766992233E-5
9	0.0	0.0	0.0	0.0	1.941941766992233E-5
10-11	0.0	0.0	0.0	0.0	1.941941766992233E-5
12-13	0.0	0.0	0.0	9.709708834961166E-6	4.8548544174805826E-5
14-15	0.0	0.0	0.0	1.941941766992233E-5	7.767767067968934E-5
16-17	0.0	0.0	0.0	1.941941766992233E-5	9.709708834961167E-5
18-19	0.0	0.0	0.0	3.883883533984466E-5	9.709708834961167E-5
20-21	0.0	0.0	0.0	5.8258253009767E-5	1.16516506019534E-4
22-23	0.0	0.0	0.0	1.2622621485449515E-4	1.16516506019534E-4
24-25	0.0	0.0	0.0	3.0100097388379614E-4	1.16516506019534E-4
26-27	0.0	0.0	0.0	6.699699096123204E-4	1.16516506019534E-4
28-29	0.0	0.0	0.0	0.0032042039155371848	1.16516506019534E-4
30-31	0.0	0.0	0.0	0.013146945762537419	1.16516506019534E-4
32-33	0.0	0.0	0.0	0.03081861584216674	1.16516506019534E-4
34-35	0.0	0.0	0.0	0.05903502971656389	1.16516506019534E-4
36-37	0.0	0.0	0.0	0.10704953990544686	1.16516506019534E-4
38-39	0.0	0.0	0.0	0.18240659017358046	1.16516506019534E-4
40-41	0.0	0.0	0.0	0.2641137900197787	1.16516506019534E-4
42-43	0.0	0.0	0.0	0.34789886755665855	1.16516506019534E-4
44-45	0.0	0.0	0.0	0.44964690643821664	1.2622621485449515E-4
46-47	0.0	0.0	0.0	0.5791355834612588	1.3593592368945634E-4
48-49	0.0	0.0	0.0	0.7292768111762633	1.3593592368945634E-4
50-51	0.0	0.0	0.0	0.889341361320598	1.74774759029301E-4
52-53	0.0	0.0	0.0	1.0453375434630843	1.74774759029301E-4
54-55	0.0	0.0	0.0	1.2190636539382094	1.74774759029301E-4
56-57	0.0	0.0	0.0	1.4293176890504584	1.74774759029301E-4
58-59	0.0	0.0	0.0	1.686207455697026	1.74774759029301E-4
60-61	0.0	0.0	0.0	1.9503212457168047	1.74774759029301E-4
62-63	0.0	0.0	0.0	2.228300499952908	2.1361359436914565E-4
64-65	0.0	0.0	0.0	2.518436309650382	2.1361359436914565E-4
66-67	0.0	0.0	0.0	2.845362206123525	2.33033012039068E-4
68-69	0.0	0.0	0.0	3.216418729251566	2.33033012039068E-4
70-71	0.0	0.0	0.0	3.6243333071171193	2.33033012039068E-4
72-73	0.0	0.0	0.0	4.0570853201825035	2.4274272087402914E-4
74-75	0.0	0.0	0.0	4.478583780708168	2.621621385439515E-4
76-77	0.0	0.0	0.0	4.955233387416412	2.7187184737891267E-4
78-79	0.0	0.0	0.0	5.470352860528772	2.7187184737891267E-4
80-81	0.0	0.0	0.0	6.030360317585156	2.7187184737891267E-4
82-83	0.0	0.0	0.0	6.614302206919721	2.7187184737891267E-4
84-85	0.0	0.0	0.0	7.1724745290062994	2.7187184737891267E-4
86-87	0.0	0.0	0.0	7.770320721392528	2.8158155621387383E-4
88	0.0	0.0	0.0	8.250048305801453	2.91291265048835E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6100	0.0	42.637398	94
GTACTAG	6240	0.0	36.392033	1
GCATCGT	6080	0.0	34.547432	8
TAGTACT	8530	0.0	33.276176	4
CTGTGCG	8240	0.0	33.190025	9
TAGGCAT	7410	0.0	32.723213	5
CAACGCA	8620	0.0	32.163937	5
TCTAACG	3190	0.0	31.968248	2
CTAACGC	3180	0.0	31.772903	3
CAGTACG	685	0.0	31.555792	9
ACTGTGC	8990	0.0	30.943815	8
TAACGCC	3345	0.0	30.486612	4
GTATCAA	10200	0.0	30.421988	1
CGCCTAC	3310	0.0	30.23872	7
ATCAACG	9230	0.0	30.141546	3
GTACTGT	9270	0.0	30.009157	6
CTAGGCA	8535	0.0	29.842918	4
TACTGTG	10190	0.0	29.374943	7
CTAGTAC	10300	0.0	28.515982	3
ACGCCTA	3675	0.0	27.619017	6
>>END_MODULE