FastQCFastQC Report
Mon 16 Jan 2017
ERR1146652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5149485
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94840.18417375718154338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81110.15751089672074003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51570.10014593692378947No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC56000.046.2755994
GGTATCA92600.040.7517171
GTATCAA159450.038.00791
CTGTGCG78700.035.3613329
TAGTACT81150.034.381054
CAGTACG7800.033.75019
ACTGTGC85500.033.255018
CAACGCA176200.032.7282875
GTACTAG60950.032.346171
ATCAACG179300.032.1184163
GTACTGT87900.031.9727556
TACTGTG98450.031.0288287
TAGGCAT77000.029.8471415
CTAGTAC98050.029.6547223
TATCAAC204600.029.6435222
CTAACGC30950.029.6433283
GTGGTAT20350.028.9020161
AACGCAG210400.028.345476
TCTAACG33200.028.2031442
CGCCTAC32400.027.849927
Produced by FastQC (version 0.11.5)