##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146652_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5149485
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.103807856513804	33.0	31.0	34.0	30.0	34.0
2	32.149328719279694	33.0	31.0	34.0	30.0	34.0
3	32.31527269231778	34.0	31.0	34.0	30.0	34.0
4	35.93427245637185	37.0	35.0	37.0	35.0	37.0
5	35.846514942756414	37.0	35.0	37.0	35.0	37.0
6	35.880360074842436	37.0	35.0	37.0	35.0	37.0
7	35.80080551744495	37.0	35.0	37.0	35.0	37.0
8	35.80849308231794	37.0	35.0	37.0	35.0	37.0
9	37.5105520260764	39.0	37.0	39.0	35.0	39.0
10-11	37.44332103113224	39.0	37.0	39.0	34.0	39.0
12-13	37.41372913990428	39.0	37.0	39.0	34.0	39.0
14-15	38.62826282628263	40.0	38.0	41.0	34.0	41.0
16-17	38.62661596256713	40.0	38.0	41.0	34.0	41.0
18-19	38.673193921333876	40.0	38.0	41.0	34.0	41.0
20-21	38.66845791375254	40.0	38.0	41.0	34.0	41.0
22-23	38.6329668889219	40.0	38.0	41.0	34.0	41.0
24-25	38.55757828210005	40.0	38.0	41.0	34.0	41.0
26-27	38.4130555774024	40.0	38.0	41.0	34.0	41.0
28-29	38.360249617194725	40.0	38.0	41.0	34.0	41.0
30-31	38.23484756242614	40.0	37.5	41.0	33.5	41.0
32-33	38.101862322154545	40.0	37.0	41.0	33.0	41.0
34-35	38.00267502478403	40.0	37.0	41.0	33.0	41.0
36-37	37.99442225775976	40.0	37.0	41.0	33.0	41.0
38-39	37.9006975454827	40.0	37.0	41.0	33.0	41.0
40-41	37.78662759479832	40.0	37.0	41.0	33.0	41.0
42-43	37.6575770198379	40.0	36.5	41.0	32.5	41.0
44-45	37.44914637094777	40.0	36.0	41.0	32.0	41.0
46-47	37.21456485454371	40.0	36.0	41.0	31.0	41.0
48-49	36.956266500436456	39.0	35.0	41.0	31.0	41.0
50-51	36.75090392534399	39.0	35.0	41.0	31.0	41.0
52-53	36.5609065760945	39.0	35.0	41.0	30.0	41.0
54-55	36.37304361504111	39.0	35.0	41.0	30.0	41.0
56-57	36.137815432028646	38.0	35.0	40.0	30.0	41.0
58-59	35.84997227878128	37.5	35.0	40.0	29.5	41.0
60-61	35.670383349014514	37.0	35.0	40.0	30.0	41.0
62-63	35.45086256198435	36.5	34.5	40.0	29.0	41.0
64-65	35.20427479641168	36.0	34.0	39.5	29.0	41.0
66-67	34.958823260966874	35.5	34.0	39.0	29.0	41.0
68-69	34.70609847392507	35.0	34.0	39.0	29.0	41.0
70-71	34.498946982076845	35.0	34.0	38.5	29.0	41.0
72-73	34.2688107645716	35.0	34.0	37.5	29.0	40.0
74-75	34.021542736797954	35.0	34.0	37.0	29.0	39.5
76-77	33.78227512071595	35.0	34.0	36.5	28.5	39.0
78-79	33.5600881447368	35.0	33.0	36.0	28.0	39.0
80-81	33.438515696229814	35.0	33.0	36.0	28.5	38.5
82-83	33.279982075877484	35.0	33.0	35.5	29.0	37.0
84-85	33.15356118136086	35.0	33.0	35.0	29.0	37.0
86-87	33.01563350509808	35.0	33.0	35.0	29.0	36.5
88-89	32.91558427687429	35.0	33.0	35.0	29.0	36.0
90-91	32.851833047382414	35.0	33.0	35.0	29.0	36.0
92-93	32.783885573023326	35.0	33.0	35.0	29.0	36.0
94-95	32.74033529566549	35.0	33.0	35.0	29.0	35.5
96-97	32.65463119127446	35.0	33.0	35.0	27.5	35.0
98-99	32.58038425201744	35.0	33.0	35.0	27.0	35.0
100	31.311975469391598	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3739719754469917
1101	2	0.4125109486340719
1101	3	0.3917400001606808
1101	4	0.2965552307319044
1101	5	0.2588710222502755
1101	6	0.2861690351180144
1101	7	0.33481048015902815
1101	8	0.28462967747033474
1101	9	0.37055380994572573
1101	10-11	0.39767324664348536
1101	12-13	0.38639638180169555
1101	14-15	0.486208807429513
1101	16-17	0.531312129210086
1101	18-19	0.5144379774611991
1101	20-21	0.5251749730025708
1101	22-23	0.4984000352026001
1101	24-25	0.498154631794705
1101	26-27	0.5946435990597081
1101	28-29	0.595102687171682
1101	30-31	0.5808339376529688
1101	32-33	0.6065618253958789
1101	34-35	0.5963865521343124
1101	36-37	0.4974178219854295
1101	38-39	0.558304169818058
1101	40-41	0.5630091274677227
1101	42-43	0.5513574048419159
1101	44-45	0.47547768672215796
1101	46-47	0.4856786812416871
1101	48-49	0.44530535505896296
1101	50-51	0.5380660910982016
1101	52-53	0.6105230656060954
1101	54-55	0.563684737264694
1101	56-57	0.4997623056522471
1101	58-59	0.43623349920254384
1101	60-61	0.4868717973696377
1101	62-63	0.4603337147509876
1101	64-65	0.5311112918230165
1101	66-67	0.4791644074614254
1101	68-69	0.46308973702018363
1101	70-71	0.4874844210927023
1101	72-73	0.4796739614046146
1101	74-75	0.5356578233800491
1101	76-77	0.4507506959974066
1101	78-79	0.4285131212832951
1101	80-81	0.4509185636670523
1101	82-83	0.43561497885756495
1101	84-85	0.4387767229628352
1101	86-87	0.39938464540761487
1101	88-89	0.4217388482666209
1101	90-91	0.3990812448807759
1101	92-93	0.395292529734121
1101	94-95	0.3958229676478382
1101	96-97	0.39810841871995706
1101	98-99	0.42962304963431563
1101	100	0.667170315931692
1102	1	0.3634623600623783
1102	2	0.41231383324945625
1102	3	0.32649961554529483
1102	4	0.2333773461165194
1102	5	0.23477486840412354
1102	6	0.21925557357955228
1102	7	0.30405086477441756
1102	8	0.31159121593186967
1102	9	0.38040957917649365
1102	10-11	0.3501900735665586
1102	12-13	0.36020888180169663
1102	14-15	0.48641313435258127
1102	16-17	0.4539948215177745
1102	18-19	0.44946922746120066
1102	20-21	0.47557881915641786
1102	22-23	0.5726019582795203
1102	24-25	0.5310777087177883
1102	26-27	0.5174512913674008
1102	28-29	0.5541844179409097
1102	30-31	0.5940142261145098
1102	32-33	0.582590671549724
1102	34-35	0.6199803021343087
1102	36-37	0.6033745527546586
1102	38-39	0.6078354198180591
1102	40-41	0.602369704390803
1102	42-43	0.5851602894572991
1102	44-45	0.6012613405683069
1102	46-47	0.5734887773955322
1102	48-49	0.5658245858281958
1102	50-51	0.5878833987905097
1102	52-53	0.6144317194522486
1102	54-55	0.5341799295723888
1102	56-57	0.4908416325753251
1102	58-59	0.4357984030486932
1102	60-61	0.474869393523484
1102	62-63	0.5463361185971465
1102	64-65	0.48602956105378325
1102	66-67	0.44457306130757246
1102	68-69	0.4612291600971048
1102	70-71	0.4579050941696252
1102	72-73	0.49791674986615675
1102	74-75	0.49368426568774026
1102	76-77	0.4169646383051031
1102	78-79	0.4262991789756043
1102	80-81	0.3906541405901294
1102	82-83	0.3641702673191034
1102	84-85	0.41355076142437497
1102	86-87	0.44551204925377164
1102	88-89	0.4265176944204754
1102	90-91	0.39421586026539046
1102	92-93	0.39065551050335046
1102	94-95	0.4110801791863068
1102	96-97	0.43585120718149994
1102	98-99	0.41049804963431313
1102	100	0.6577616620855373
1103	1	0.2664209974591998
1103	2	0.3411467154722132
1103	3	0.2315297477381435
1103	4	0.20998807257901575
1103	5	0.23273888922000907
1103	6	0.22838420738742116
1103	7	0.23968536052749556
1103	8	0.195258520633395
1103	9	0.26657905219958167
1103	10-11	0.3051705773648621
1103	12-13	0.2922078873645617
1103	14-15	0.4122263484033084
1103	16-17	0.33511864763333676
1103	18-19	0.343492791135084
1103	20-21	0.3578884928614059
1103	22-23	0.3608797501804659
1103	24-25	0.35560161259384415
1103	26-27	0.46838553888504464
1103	28-29	0.45633946788707647
1103	30-31	0.4050781803853525
1103	32-33	0.44561499413616446
1103	34-35	0.48550969036854497
1103	36-37	0.42761937869077826
1103	38-39	0.41746005718496804
1103	40-41	0.41561553224102
1103	42-43	0.35781177288833277
1103	44-45	0.4905507666360194
1103	46-47	0.4607759641104465
1103	48-49	0.452020789022356
1103	50-51	0.47376519535708894
1103	52-53	0.4082628445868366
1103	54-55	0.31008296073640196
1103	56-57	0.32993076928426746
1103	58-59	0.4198899323883296
1103	60-61	0.4412836812672296
1103	62-63	0.3648465751409873
1103	64-65	0.3622675605154413
1103	66-67	0.39369825570882
1103	68-69	0.3785761065021731
1103	70-71	0.3764791049663714
1103	72-73	0.3156200673981573
1103	74-75	0.3002285779559557
1103	76-77	0.3485010997538396
1103	78-79	0.32191458976038945
1103	80-81	0.3047200052270469
1103	82-83	0.3025522695023781
1103	84-85	0.3229620247333784
1103	86-87	0.28490101249698085
1103	88-89	0.28343688331866446
1103	90-91	0.30660071491307406
1103	92-93	0.3154851290282963
1103	94-95	0.2776120422979176
1103	96-97	0.29590651807394863
1103	98-99	0.30667096206403244
1103	100	0.4928411796713732
1104	1	0.2503384241391444
1104	2	0.28385727660187854
1104	3	0.21099553335987764
1104	4	0.21469481825705117
1104	5	0.1476879236587223
1104	6	0.1957210974922603
1104	7	0.2410526833783493
1104	8	0.20014174990494382
1104	9	0.26529727071031317
1104	10-11	0.2988564709895556
1104	12-13	0.28794089890431707
1104	14-15	0.3575078516951038
1104	16-17	0.32847221472316335
1104	18-19	0.35089182554973064
1104	20-21	0.3630611651554858
1104	22-23	0.365605895363565
1104	24-25	0.401887906442596
1104	26-27	0.4342042127417969
1104	28-29	0.4239527877752991
1104	30-31	0.3967954567676557
1104	32-33	0.44700963726710796
1104	34-35	0.4626409535427598
1104	36-37	0.42465512077818346
1104	38-39	0.3975188327959245
1104	40-41	0.5005786697212429
1104	42-43	0.5016012428700805
1104	44-45	0.47481395432778584
1104	46-47	0.4800716641390679
1104	48-49	0.4860582474130908
1104	50-51	0.47355439592718085
1104	52-53	0.49409285936867064
1104	54-55	0.46468725235524033
1104	56-57	0.41299583180919086
1104	58-59	0.42672872053050526
1104	60-61	0.4286510981744627
1104	62-63	0.4256687248113522
1104	64-65	0.40005143266808574
1104	66-67	0.37157449297450285
1104	68-69	0.39792965150709847
1104	70-71	0.3263318808512494
1104	72-73	0.30345514852734823
1104	74-75	0.3286825970218956
1104	76-77	0.3226181507257806
1104	78-79	0.3452558275207238
1104	80-81	0.27650457976363185
1104	82-83	0.28158618107084266
1104	84-85	0.3275414362425195
1104	86-87	0.31750373494483597
1104	88-89	0.33726777180786627
1104	90-91	0.319952975263071
1104	92-93	0.3129908446234637
1104	94-95	0.2839937422957277
1104	96-97	0.2830740877340361
1104	98-99	0.29640725486569863
1104	100	0.5299333944025157
1105	1	0.17513275640530424
1105	2	0.19625106997819586
1105	3	0.1276967518719445
1105	4	0.1340095711177156
1105	5	0.14380537197025234
1105	6	0.14321236119144487
1105	7	0.17554546771348356
1105	8	0.15183222880823166
1105	9	0.2548568591056508
1105	10-11	0.15780777526824608
1105	12-13	0.20465797172079903
1105	14-15	0.27722350873629154
1105	16-17	0.27106115690143895
1105	18-19	0.2067610561793103
1105	20-21	0.2702317947200612
1105	22-23	0.23151885131958494
1105	24-25	0.23034959912513386
1105	26-27	0.24478228916549227
1105	28-29	0.24187446688542735
1105	30-31	0.30347340560567915
1105	32-33	0.19397902359128238
1105	34-35	0.15665432593642947
1105	36-37	0.206682795538633
1105	38-39	0.30993706496429496
1105	40-41	0.2840787836593819
1105	42-43	0.26438669077844423
1105	44-45	0.2214166257389536
1105	46-47	0.23819902193863385
1105	48-49	0.2608575096949366
1105	50-51	0.23976541437138366
1105	52-53	0.20615767201555713
1105	54-55	0.19465595692866344
1105	56-57	0.16293603776177434
1105	58-59	0.21427713641473645
1105	60-61	0.21000492586994568
1105	62-63	0.15440901033282017
1105	64-65	0.17437404851249738
1105	66-67	0.1675392907843829
1105	68-69	0.16447652140101354
1105	70-71	0.20603859813066805
1105	72-73	0.2133799352689536
1105	74-75	0.2318550464665492
1105	76-77	0.17194274640188922
1105	78-79	0.17702268880041316
1105	80-81	0.07585089098503772
1105	82-83	0.13448916211829953
1105	84-85	0.1516099136908977
1105	86-87	0.22967295436841084
1105	88-89	0.24613080532947862
1105	90-91	0.18712309304505936
1105	92-93	0.18006411498614483
1105	94-95	0.17721119104547967
1105	96-97	0.2019493101325267
1105	98-99	0.24460469244701244
1105	100	0.43629959408974983
1106	1	0.1660250126046705
1106	2	0.19761923974277096
1106	3	0.156244463907818
1106	4	0.16234047322195266
1106	5	0.10983814735583763
1106	6	0.16544161021754178
1106	7	0.203942809149261
1106	8	0.14721883177121242
1106	9	0.1855043468017996
1106	10-11	0.15704208637082928
1106	12-13	0.1349444123240744
1106	14-15	0.2194063433204505
1106	16-17	0.16157534702887943
1106	18-19	0.24280536948677423
1106	20-21	0.22285734572545834
1106	22-23	0.24164392017062397
1106	24-25	0.2073506973635375
1106	26-27	0.243742419785967
1106	28-29	0.18220073880637955
1106	30-31	0.24183543059542245
1106	32-33	0.2781568383648789
1106	34-35	0.2929937650067984
1106	36-37	0.22478743080434782
1106	38-39	0.239863276767025
1106	40-41	0.2242285221474205
1106	42-43	0.21190408568399022
1106	44-45	0.24665118473156156
1106	46-47	0.2559569189663833
1106	48-49	0.2524848926992078
1106	50-51	0.26169990549077227
1106	52-53	0.21396843748448902
1106	54-55	0.18588708374798557
1106	56-57	0.1290512804984303
1106	58-59	0.04212357942934375
1106	60-61	0.12146940413764185
1106	62-63	0.17851841736891316
1106	64-65	0.20180772685739612
1106	66-67	0.23274192736732147
1106	68-69	0.2051895002606159
1106	70-71	0.2289418873129918
1106	72-73	0.17861454531099952
1106	74-75	0.13780012794030938
1106	76-77	0.20853081121034478
1106	78-79	0.20314143893319425
1106	80-81	0.13589428450189445
1106	82-83	0.13459201921225628
1106	84-85	0.13839978515369467
1106	86-87	0.08424387903048824
1106	88-89	0.13050409437592947
1106	90-91	0.13398566640327658
1106	92-93	0.1345989071620366
1106	94-95	0.1481412358345935
1106	96-97	0.12364481484735279
1106	98-99	0.08000168396897323
1106	100	0.19785860764359953
1107	1	0.16241811057529532
1107	2	0.19255928323146776
1107	3	0.17501929481369416
1107	4	0.17046259073696035
1107	5	0.0465127236786671
1107	6	0.05805433722899522
1107	7	0.11232749760309702
1107	8	0.10901325729589217
1107	9	0.09206499690059644
1107	10-11	0.14442513483713526
1107	12-13	0.054783485202030135
1107	14-15	0.16986455575462145
1107	16-17	0.1562028746910613
1107	18-19	0.14333763458419213
1107	20-21	0.14658166147431473
1107	22-23	0.12394218379316868
1107	24-25	0.08614746613929469
1107	26-27	0.20194291330569314
1107	28-29	0.258785439036167
1107	30-31	0.2075478328621685
1107	32-33	0.28227430643494245
1107	34-35	0.27345143805264627
1107	36-37	0.12981205943960816
1107	38-39	0.176208867391054
1107	40-41	0.23185819048124756
1107	42-43	0.32980716625008455
1107	44-45	0.23879052643900422
1107	46-47	0.320279387536722
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2216	88-89	-0.9214709284990938
2216	90-91	-0.8824536588074423
2216	92-93	-0.8978729081485106
2216	94-95	-0.8832233030703946
2216	96-97	-0.9304720370811523
2216	98-99	-0.9540471758397793
2216	100	-0.40831225088927425
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	6.0
12	10.0
13	76.0
14	247.0
15	770.0
16	1848.0
17	4005.0
18	6892.0
19	10943.0
20	15455.0
21	22244.0
22	30150.0
23	35763.0
24	37083.0
25	37607.0
26	40964.0
27	50384.0
28	63143.0
29	80669.0
30	100883.0
31	126112.0
32	158946.0
33	203551.0
34	290021.0
35	417891.0
36	675837.0
37	1247877.0
38	1071353.0
39	418071.0
40	682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.11154513509603	21.718734980294148	12.626505368983501	25.54321451562632
2	14.573461579468965	20.766315133669348	39.288536520804065	25.371686766057618
3	17.193175628242436	27.142617174338795	29.62573927295642	26.03846792446235
4	11.46692851510439	17.119585551766086	39.538569874484956	31.874916058644565
5	12.196569947745799	39.16868686339042	35.63871716488476	12.99602602397902
6	29.566055466150683	39.41013107529435	17.61137962781895	13.412433830736017
7	25.967514415036536	32.56576775459445	24.104240347188185	17.36247748318084
8	26.77729908913221	34.56947636511224	19.529098540922053	19.124126004833496
9	26.438818639145467	13.890534684536416	21.455970839802426	38.21467583651569
10-11	25.679276665530633	25.766197979021204	27.675097606848066	20.8794277486001
12-13	25.785370193203512	23.65527574555539	28.23207996401181	22.327274097229296
14-15	23.08363538338945	24.765608962239323	25.763932708238773	26.386822946132447
16-17	21.487148714871488	27.132897755795	27.233995244184612	24.145958285148904
18-19	20.853231782626594	27.48672681513473	29.727720080474146	21.93232132176453
20-21	22.45793511389974	26.641343746025086	28.911493091056677	21.989228049018493
22-23	22.93373997593934	26.29892115425135	28.504442677277435	22.262896192531876
24-25	22.440418799161467	26.22851605548904	29.54455639738731	21.786508747962174
26-27	22.470810187814898	26.454548367458102	28.309607659795105	22.76503378493189
28-29	21.554281641756408	27.115876636207307	28.83497087572835	22.494870846307933
30-31	22.49390958513327	26.750170162647336	28.813327934735224	21.942592317484173
32-33	23.082240262861237	26.849053837422577	27.922335922912676	22.146369976803506
34-35	22.834875720581767	26.68571711540086	28.837903207796504	21.641503956220866
36-37	22.494725200675408	26.11309674656786	28.485809746023143	22.906368306733587
38-39	22.709533089231254	26.2071741154698	29.301735998842602	21.781556796456343
40-41	22.161186992485657	25.49359790347967	29.736993116787406	22.60822198724727
42-43	22.402075158972206	25.66083792845304	28.730416730993486	23.206670181581266
44-45	22.559226796466053	26.713147042859624	28.5521464767836	22.17547968389072
46-47	22.625165429164277	26.51651572924283	27.774796897165444	23.083521944427453
48-49	23.59329136797175	26.37805528125628	27.6566103212263	22.372043029545672
50-51	22.791444192962988	26.18926941237813	27.559804524141736	23.45948187051715
52-53	22.04182554177748	26.91458466234973	27.419139972249653	23.624449823623138
54-55	22.152302608901667	26.942393268453056	27.195855507880886	23.70944861476439
56-57	23.562094073485017	26.16071315869451	26.92195433135546	23.355238436465005
58-59	22.118004650369304	26.7192913619395	28.001623852178575	23.16108013551262
60-61	22.335660750541074	27.454551280370755	27.031256523710624	23.178531445377548
62-63	22.880152092879193	26.49450381931397	26.593533139721735	24.0318109480851
64-65	21.928144013750515	26.81985105099437	27.046029206247113	24.205975729008003
66-67	22.564253886488807	26.348834235415673	27.09967784091367	23.98723403718185
68-69	22.1272321406898	26.251207645036352	27.498526551684293	24.12303366258956
70-71	22.066960885561848	25.985939480185667	27.548542039602648	24.39855759464984
72-73	22.697349480649677	25.825258461441365	28.124090621108515	23.353301436800443
74-75	22.832256764662333	26.327912864692188	27.4678570821712	23.371973288474283
76-77	22.549532700116927	26.27056106862891	26.983866516929776	24.196039714324385
78-79	22.179372181634545	26.368258716457554	27.141190678753574	24.311178423154324
80-81	21.75389383598554	26.88242610668834	27.820617013157626	23.543063044168495
82-83	22.708649505727273	25.59882201812414	28.89061721706151	22.801911259087074
84-85	23.080987710421528	26.22017541559981	27.25872587258726	23.4401110013914
86-87	21.3842258012209	26.923129206124496	27.888623813837693	23.80402117881691
88-89	22.53301058261166	25.750312895367205	27.503963988631874	24.21271253338926
90-91	22.050544860311273	25.6546334245075	27.781651951602925	24.5131697635783
92-93	22.630787350579716	25.959751314937318	27.358726163878522	24.05073517060444
94-95	21.264728414589033	26.037477534161184	28.07128285644098	24.6265111948088
96-97	21.66582677685244	25.807736113417167	27.634821734600646	24.891615375129746
98-99	20.89623525459342	26.547499410135188	27.629209522894037	24.927055812377354
100	21.84945063956351	25.743572982294005	26.576503750559155	25.83047262758333
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	275.0
1	185.0
2	143.5
3	182.0
4	113.0
5	85.5
6	202.5
7	440.0
8	699.5
9	750.5
10	607.0
11	496.5
12	501.0
13	580.5
14	745.5
15	1147.5
16	1557.0
17	2063.5
18	2861.5
19	3731.5
20	4761.5
21	5954.0
22	7241.0
23	8650.0
24	9984.5
25	11608.5
26	14442.5
27	17199.0
28	21316.5
29	31608.5
30	46499.5
31	59828.0
32	78083.5
33	94985.0
34	105440.5
35	119796.5
36	132734.5
37	155195.5
38	165385.0
39	154568.0
40	144150.0
41	132469.5
42	129559.0
43	137272.0
44	150680.0
45	182221.0
46	213997.0
47	233081.0
48	246374.5
49	241291.0
50	227262.5
51	232604.5
52	238208.0
53	228939.0
54	219369.5
55	186711.5
56	159541.5
57	131841.5
58	101152.5
59	100435.0
60	83022.5
61	52967.0
62	37927.5
63	31333.0
64	22275.5
65	9502.0
66	4504.0
67	2952.0
68	1970.0
69	1362.0
70	933.5
71	536.5
72	287.5
73	140.0
74	58.0
75	24.5
76	11.5
77	3.0
78	0.5
79	0.5
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0062918913250548355
3	0.0
4	0.08785344553872863
5	0.004544143734761826
6	3.883883533984466E-5
7	1.941941766992233E-5
8	0.0
9	0.0
10-11	0.0
12-13	3.5925922689356314E-4
14-15	0.009787386505640854
16-17	0.0
18-19	9.709708834961165E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.91291265048835E-5
60-61	0.0
62-63	0.0
64-65	8.73873795146505E-5
66-67	2.0390388553418452E-4
68-69	0.0
70-71	6.020019477675923E-4
72-73	4.369368975732525E-4
74-75	4.369368975732525E-4
76-77	1.5535534135937865E-4
78-79	3.883883533984466E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06445304724647222
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5149485.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.65941351748301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.1701057714809	43.883069818467455
2	16.500654787712303	20.348413937295774
3	6.028112605794282	11.150696636718651
4	2.682174049882102	6.615251154701705
5	1.3754993633252017	4.2406242018151605
6	0.7975581388382992	2.950618025211889
7	0.4344736135446008	1.8752571739987154
8	0.28184993913674	1.3902961557687736
9	0.18687125194789964	1.0370134618546767
>10	0.5324390071139287	4.679327621737299
>50	0.0055193653529453475	0.2352193091401738
>100	0.0038124845330483846	0.480515396735777
>500	5.577728100037905E-4	0.25154841058614524
>1k	2.32405329751683E-4	0.21007745300990946
>5k	1.394431978510098E-4	0.6520712429579073
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9484	0.18417375718154338	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8111	0.15751089672074003	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5157	0.10014593692378947	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	9.709708834961165E-5
14-15	0.0	0.0	0.0	0.0	1.16516506019534E-4
16-17	0.0	0.0	0.0	0.0	1.16516506019534E-4
18-19	0.0	0.0	0.0	1.941941766992233E-5	1.16516506019534E-4
20-21	0.0	0.0	0.0	2.91291265048835E-5	1.2622621485449515E-4
22-23	0.0	0.0	0.0	8.73873795146505E-5	1.3593592368945634E-4
24-25	0.0	0.0	0.0	2.6216213854395146E-4	1.3593592368945634E-4
26-27	0.0	0.0	0.0	6.311310742724758E-4	1.3593592368945634E-4
28-29	0.0	0.0	0.0	0.0031459456625274177	1.3593592368945634E-4
30-31	0.0	0.0	0.0	0.013166365180207341	1.3593592368945634E-4
32-33	0.0	0.0	0.0	0.031109907107215576	1.5535534135937865E-4
34-35	0.0	0.0	0.0	0.059685580208506284	1.5535534135937865E-4
36-37	0.0	0.0	0.0	0.10811760787729258	1.5535534135937865E-4
38-39	0.0	0.0	0.0	0.18387275620765958	1.5535534135937865E-4
40-41	0.0	0.0	0.0	0.2658421181924018	1.5535534135937865E-4
42-43	0.0	0.0	0.0	0.3502777462212241	1.5535534135937865E-4
44-45	0.0	0.0	0.0	0.45267633559472453	1.5535534135937865E-4
46-47	0.0	0.0	0.0	0.5827767242743691	1.5535534135937865E-4
48-49	0.0	0.0	0.0	0.7334714053929665	1.5535534135937865E-4
50-51	0.0	0.0	0.0	0.8942544739910885	1.5535534135937865E-4
52-53	0.0	0.0	0.0	1.0514158211937699	1.74774759029301E-4
54-55	0.0	0.0	0.0	1.2262197093495757	1.74774759029301E-4
56-57	0.0	0.0	0.0	1.4383962668111472	1.9419417669922333E-4
58-59	0.0	0.0	0.0	1.6974124596925713	1.9419417669922333E-4
60-61	0.0	0.0	0.0	1.9641381613889544	1.9419417669922333E-4
62-63	0.0	0.0	0.0	2.244272970986419	2.1361359436914565E-4
64-65	0.0	0.0	0.0	2.5373799515873916	2.2332330320410683E-4
66-67	0.0	0.0	0.0	2.8674906325584013	2.33033012039068E-4
68-69	0.0	0.0	0.0	3.242673781941301	2.33033012039068E-4
70-71	0.0	0.0	0.0	3.6540450161521005	2.33033012039068E-4
72-73	0.0	0.0	0.0	4.09048671857477	2.33033012039068E-4
74-75	0.0	0.0	0.0	4.51559719078704	2.33033012039068E-4
76-77	0.0	0.0	0.0	4.9962180684087825	2.33033012039068E-4
78-79	0.0	0.0	0.0	5.516231234773963	2.33033012039068E-4
80-81	0.0	0.0	0.0	6.082472324902393	2.33033012039068E-4
82-83	0.0	0.0	0.0	6.67334694634512	2.33033012039068E-4
84-85	0.0	0.0	0.0	7.238626775298889	2.33033012039068E-4
86-87	0.0	0.0	0.0	7.843939733779203	2.524524297089903E-4
88	0.0	0.0	0.0	8.330367017284253	2.7187184737891267E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5600	0.0	46.27559	94
GGTATCA	9260	0.0	40.751717	1
GTATCAA	15945	0.0	38.0079	1
CTGTGCG	7870	0.0	35.361332	9
TAGTACT	8115	0.0	34.38105	4
CAGTACG	780	0.0	33.7501	9
ACTGTGC	8550	0.0	33.25501	8
CAACGCA	17620	0.0	32.728287	5
GTACTAG	6095	0.0	32.34617	1
ATCAACG	17930	0.0	32.118416	3
GTACTGT	8790	0.0	31.972755	6
TACTGTG	9845	0.0	31.028828	7
TAGGCAT	7700	0.0	29.847141	5
CTAGTAC	9805	0.0	29.654722	3
TATCAAC	20460	0.0	29.643522	2
CTAACGC	3095	0.0	29.643328	3
GTGGTAT	2035	0.0	28.902016	1
AACGCAG	21040	0.0	28.34547	6
TCTAACG	3320	0.0	28.203144	2
CGCCTAC	3240	0.0	27.84992	7
>>END_MODULE