FastQCFastQC Report
Mon 16 Jan 2017
ERR1146651_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146651_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4994065
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC53950.040.2571794
CAACGCA74300.033.837425
CTGTGCG69700.033.237559
GTACTAG55950.032.691441
TAGTACT74500.031.9832884
GCATCGT54200.031.4718468
GTATCAA90550.031.208331
ATCAACG81250.031.0036493
CTAACGC25550.030.1664583
TAGGCAT68000.029.8543135
TCTAACG26200.029.7771112
ACTGTGC82450.029.3515768
CAGTACG8500.028.7474489
GGCGTCG39450.028.587698
CTAGTAC85800.028.5378883
TAACGCC26950.028.4249844
GTACTGT86700.028.0217346
ATACGGG41300.027.8796583
TACTGTG93100.027.7103427
CGCCTAC27450.027.5614937
Produced by FastQC (version 0.11.5)