##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146651_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4994065
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226490644394897	31.0	31.0	34.0	28.0	34.0
2	31.228983002824354	31.0	31.0	34.0	28.0	34.0
3	31.395044517842678	31.0	31.0	34.0	28.0	34.0
4	34.8684824887141	37.0	35.0	37.0	32.0	37.0
5	35.003188584850214	37.0	35.0	37.0	32.0	37.0
6	35.08457218718619	37.0	35.0	37.0	32.0	37.0
7	34.97881325132933	37.0	35.0	37.0	32.0	37.0
8	34.963695706803975	37.0	35.0	37.0	32.0	37.0
9	36.459401309354206	39.0	37.0	39.0	32.0	39.0
10-11	36.3270104614177	39.0	37.0	39.0	31.0	39.0
12-13	36.26893762896558	39.0	37.0	39.0	31.0	39.0
14-15	37.414270338892265	40.0	37.0	41.0	31.0	41.0
16-17	37.428718689083944	40.0	37.0	41.0	31.0	41.0
18-19	37.37711633709213	40.0	37.0	41.0	31.0	41.0
20-21	37.45298259033473	40.0	37.0	41.0	31.0	41.0
22-23	37.372956699602426	40.0	37.0	41.0	30.5	41.0
24-25	37.3161131262809	40.0	37.0	41.0	30.0	41.0
26-27	37.362563062355015	40.0	37.0	41.0	30.5	41.0
28-29	37.339685506696455	40.0	37.0	41.0	30.0	41.0
30-31	37.1969019225821	40.0	36.0	41.0	30.0	41.0
32-33	37.193702625015895	40.0	36.0	41.0	30.0	41.0
34-35	37.13165537493004	40.0	36.0	41.0	30.0	41.0
36-37	37.00909369421503	40.0	36.0	41.0	30.0	41.0
38-39	36.90104494034419	40.0	36.0	41.0	30.0	41.0
40-41	36.819536890288774	39.5	35.0	41.0	30.0	41.0
42-43	36.648637332513694	39.0	35.0	41.0	29.5	41.0
44-45	36.49324227858468	39.0	35.0	41.0	29.0	41.0
46-47	36.50352077916487	39.0	35.0	41.0	29.0	41.0
48-49	36.36934481229219	39.0	35.0	41.0	29.0	41.0
50-51	36.19309019806511	39.0	35.0	41.0	28.0	41.0
52-53	35.98669320483414	38.5	34.5	40.0	28.0	41.0
54-55	35.771941594672874	38.0	34.0	40.0	28.0	41.0
56-57	35.536203473523074	38.0	34.0	40.0	28.0	41.0
58-59	35.245355937497806	37.0	34.0	40.0	27.0	41.0
60-61	34.996219212204885	37.0	33.5	40.0	27.0	41.0
62-63	34.6965932561951	36.0	33.0	40.0	26.0	41.0
64-65	34.54904531679104	36.0	33.0	39.0	26.0	41.0
66-67	34.28488936367468	35.0	33.0	39.0	26.0	41.0
68-69	34.1615548656255	35.0	33.0	39.0	26.0	41.0
70-71	34.0744853140678	35.0	33.0	38.5	26.0	41.0
72-73	33.843193470649666	35.0	33.0	37.5	26.0	40.0
74-75	33.58619781680855	35.0	33.0	37.0	26.0	39.5
76-77	33.3171517591381	35.0	33.0	36.5	26.0	39.0
78-79	33.05744498719981	35.0	33.0	36.0	26.0	39.0
80-81	32.86040850489531	35.0	32.5	36.0	25.5	38.5
82-83	32.602400849808724	35.0	32.0	35.5	25.0	37.0
84-85	32.41955391049176	35.0	32.0	35.0	25.0	37.0
86-87	32.24459703668254	35.0	32.0	35.0	25.0	36.5
88-89	32.113656310040014	35.0	32.0	35.0	25.0	36.0
90-91	31.960866148117816	35.0	32.0	35.0	24.5	36.0
92-93	31.810055535921137	35.0	32.0	35.0	24.0	36.0
94-95	31.73095634518173	35.0	32.0	35.0	24.0	35.0
96-97	31.626177272422368	35.0	31.5	35.0	24.0	35.0
98-99	31.53603647529618	35.0	31.0	35.0	23.5	35.0
100	30.549839659676035	34.0	29.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4079138901000263
1101	2	0.5158969547326819
1101	3	0.5622583058950177
1101	4	0.6723300857739503
1101	5	0.5226815697948979
1101	6	0.5428714394056655
1101	7	0.5371405496965167
1101	8	0.5683736620609139
1101	9	0.7504020524194885
1101	10-11	0.7646728605176207
1101	12-13	0.7246145853796477
1101	14-15	0.8989428098840264
1101	16-17	0.918925420273105
1101	18-19	0.8695689765579928
1101	20-21	0.8690640889585808
1101	22-23	0.840888447229716
1101	24-25	0.904213045964589
1101	26-27	0.8609342955218651
1101	28-29	0.8583090613418136
1101	30-31	0.8963475695504499
1101	32-33	0.8216955538301676
1101	34-35	0.9229882267046321
1101	36-37	0.9576323113107108
1101	38-39	0.8417608246368147
1101	40-41	0.8192838716646733
1101	42-43	0.8298113961564937
1101	44-45	0.8424000316922147
1101	46-47	0.8396613079310242
1101	48-49	0.8242758146365503
1101	50-51	0.731221495473406
1101	52-53	0.6938481152205114
1101	54-55	0.7189335389845652
1101	56-57	0.6674612995368889
1101	58-59	0.6022798293023683
1101	60-61	0.6930784740479581
1101	62-63	0.7076059456789281
1101	64-65	0.7383514872155033
1101	66-67	0.7616144543260148
1101	68-69	0.7681369608427175
1101	70-71	0.6741080997047959
1101	72-73	0.7010359372376342
1101	74-75	0.6310371770831367
1101	76-77	0.6694863391998069
1101	78-79	0.7101914072858904
1101	80-81	0.6216674526698753
1101	82-83	0.6541934916330874
1101	84-85	0.611273020320624
1101	86-87	0.6194696896379241
1101	88-89	0.6687760770891984
1101	90-91	0.6400984698469223
1101	92-93	0.647182725483443
1101	94-95	0.6481964206467978
1101	96-97	0.6273048250892366
1101	98-99	0.6734936933158586
1101	100	0.7248932098987773
1102	1	0.40776745799403
1102	2	0.46472240092982275
1102	3	0.4786142671965443
1102	4	0.6026574204403232
1102	5	0.5046207348289329
1102	6	0.5257586092601798
1102	7	0.507548472515019
1102	8	0.47563851229621434
1102	9	0.6530243653061873
1102	10-11	0.7437467363566554
1102	12-13	0.690270769527281
1102	14-15	0.9073674697239227
1102	16-17	0.8330589124936836
1102	18-19	0.8614070866076133
1102	20-21	0.802358945993717
1102	22-23	0.8423858249168319
1102	24-25	0.8742096123841705
1102	26-27	0.7712565471427197
1102	28-29	0.7881327032261041
1102	30-31	0.8376806403158668
1102	32-33	0.7812501309275106
1102	34-35	0.8308791331025418
1102	36-37	0.8043517945231997
1102	38-39	0.8407636051636942
1102	40-41	0.7569359085857528
1102	42-43	0.8541274067399982
1102	44-45	0.7971280719256981
1102	46-47	0.742603987335599
1102	48-49	0.670719096600827
1102	50-51	0.7218241224990436
1102	52-53	0.7241604700507551
1102	54-55	0.655166127028977
1102	56-57	0.6990163411774617
1102	58-59	0.6940670752342442
1102	60-61	0.7063760914124515
1102	62-63	0.7052285615285996
1102	64-65	0.6615091356841987
1102	66-67	0.7176074659799951
1102	68-69	0.6067647068654836
1102	70-71	0.5834745367928491
1102	72-73	0.6095978729690898
1102	74-75	0.5603618389427965
1102	76-77	0.5900358803792045
1102	78-79	0.6290557673944193
1102	80-81	0.6735023647769509
1102	82-83	0.5951857627108978
1102	84-85	0.6467600838014604
1102	86-87	0.6202363542848275
1102	88-89	0.645750116528248
1102	90-91	0.5383190672899083
1102	92-93	0.5852969155960785
1102	94-95	0.6637564645427716
1102	96-97	0.6325843589301989
1102	98-99	0.5689044101262617
1102	100	0.6975086556246417
1103	1	0.435193811930322
1103	2	0.3908043791814606
1103	3	0.4119536247477704
1103	4	0.5164034100276425
1103	5	0.3967623563106244
1103	6	0.4367241751780142
1103	7	0.4326200251296157
1103	8	0.44841622707127016
1103	9	0.5689998164179215
1103	10-11	0.6341419858267372
1103	12-13	0.5827068396855068
1103	14-15	0.7135708847208093
1103	16-17	0.7396558184500392
1103	18-19	0.7589774500736581
1103	20-21	0.6792112260266165
1103	22-23	0.7541383627354961
1103	24-25	0.7629366143312879
1103	26-27	0.6595194104842079
1103	28-29	0.6830255623105685
1103	30-31	0.7556558212060338
1103	32-33	0.720072528568906
1103	34-35	0.7121835572694621
1103	36-37	0.786033714082123
1103	38-39	0.8236190617359611
1103	40-41	0.717242139884469
1103	42-43	0.7438685410214205
1103	44-45	0.7035686788142215
1103	46-47	0.7322272887165369
1103	48-49	0.722302213643367
1103	50-51	0.6544079482888137
1103	52-53	0.6739932779680942
1103	54-55	0.6815493295325936
1103	56-57	0.7111088732978317
1103	58-59	0.6666421405288929
1103	60-61	0.7092830103001617
1103	62-63	0.6471083165366167
1103	64-65	0.6370102878325667
1103	66-67	0.6917018096401151
1103	68-69	0.653828508408786
1103	70-71	0.6073294712217105
1103	72-73	0.5692300363619012
1103	74-75	0.5735252893316272
1103	76-77	0.5548616943265188
1103	78-79	0.5892605821203247
1103	80-81	0.5286856766515129
1103	82-83	0.5483265641776498
1103	84-85	0.605062694959301
1103	86-87	0.5417011234965088
1103	88-89	0.6536186166676359
1103	90-91	0.6210114759338516
1103	92-93	0.5490550170671504
1103	94-95	0.5104725778830215
1103	96-97	0.5163692314378459
1103	98-99	0.5229954618385015
1103	100	0.6448030938823521
1104	1	0.30362875935458433
1104	2	0.39740897242043616
1104	3	0.3632756111060296
1104	4	0.4540838836485719
1104	5	0.40224106172583163
1104	6	0.34993201736988766
1104	7	0.3809394617180999
1104	8	0.450099060975532
1104	9	0.5038167355895382
1104	10-11	0.6370409966251458
1104	12-13	0.4500340535799481
1104	14-15	0.6449601799328519
1104	16-17	0.6558017940241712
1104	18-19	0.599841781636087
1104	20-21	0.578769628052541
1104	22-23	0.6011603445785028
1104	24-25	0.640623851833702
1104	26-27	0.5996582874560445
1104	28-29	0.5654202452153569
1104	30-31	0.5520648053850081
1104	32-33	0.6199981972676127
1104	34-35	0.5856294596603959
1104	36-37	0.5892919322688854
1104	38-39	0.5417054531485874
1104	40-41	0.6219549624311185
1104	42-43	0.5612312144809621
1104	44-45	0.5234148082287078
1104	46-47	0.5211155551316509
1104	48-49	0.5514412029177649
1104	50-51	0.5170493121898829
1104	52-53	0.5787217463322847
1104	54-55	0.5938309007341545
1104	56-57	0.6281558421385682
1104	58-59	0.6167259847445266
1104	60-61	0.615636649771389
1104	62-63	0.6453610662448312
1104	64-65	0.5491223439174675
1104	66-67	0.567295996947152
1104	68-69	0.5254207753806526
1104	70-71	0.5041558851694532
1104	72-73	0.4951629934066233
1104	74-75	0.4577405311429601
1104	76-77	0.42254114011531385
1104	78-79	0.4238296187997861
1104	80-81	0.48030880881747606
1104	82-83	0.5244292743226922
1104	84-85	0.4717266580458528
1104	86-87	0.43045370063441624
1104	88-89	0.41462204839198336
1104	90-91	0.4749171833351262
1104	92-93	0.46542876551430723
1104	94-95	0.4751054726532047
1104	96-97	0.4660615925354641
1104	98-99	0.46047413174911966
1104	100	0.45893779237571053
1105	1	0.2135209877689519
1105	2	0.32884700136052203
1105	3	0.31515651004337286
1105	4	0.384479013333646
1105	5	0.2635536623531607
1105	6	0.2489940506286601
1105	7	0.3368441469983452
1105	8	0.3202203324492814
1105	9	0.4795370140215027
1105	10-11	0.5165646951271583
1105	12-13	0.39416522257347
1105	14-15	0.5481834719193657
1105	16-17	0.5164454110595074
1105	18-19	0.5148268373079077
1105	20-21	0.44480083281578686
1105	22-23	0.5053485720291775
1105	24-25	0.5040620053559692
1105	26-27	0.4471593600250472
1105	28-29	0.535601935360269
1105	30-31	0.5539107170815782
1105	32-33	0.5371145245626039
1105	34-35	0.5214373271792354
1105	36-37	0.4750141941156798
1105	38-39	0.5064669572774179
1105	40-41	0.5260964869346054
1105	42-43	0.5201424058291764
1105	44-45	0.4920765080671714
1105	46-47	0.5254276760622147
1105	48-49	0.40975829680520093
1105	50-51	0.3897405217113601
1105	52-53	0.48860781789004903
1105	54-55	0.4650630044199815
1105	56-57	0.49926075024014693
1105	58-59	0.5100273447503412
1105	60-61	0.49744747061365757
1105	62-63	0.4215324416487789
1105	64-65	0.42314849215662065
1105	66-67	0.4046603239632134
1105	68-69	0.32182551086886946
1105	70-71	0.38475464308628204
1105	72-73	0.36213469068273696
1105	74-75	0.2900803866141004
1105	76-77	0.3576933908007476
1105	78-79	0.41534407147106833
1105	80-81	0.2908383648959685
1105	82-83	0.39183264334232604
1105	84-85	0.3033211341725277
1105	86-87	0.3203267061280215
1105	88-89	0.3065199294924952
1105	90-91	0.28607383947052156
1105	92-93	0.3951075706838161
1105	94-95	0.34692536013938025
1105	96-97	0.2813820977365289
1105	98-99	0.34711751622284837
1105	100	0.37761080514993495
1106	1	0.09221168381774802
1106	2	0.20339699938111977
1106	3	0.14123830826544292
1106	4	0.2807054184048212
1106	5	0.16636466005130757
1106	6	0.23312384108574946
1106	7	0.29814655207019314
1106	8	0.20911953451575016
1106	9	0.35586442352756364
1106	10-11	0.3976400577159751
1106	12-13	0.2716277428684961
1106	14-15	0.44490478889763807
1106	16-17	0.29151694551245555
1106	18-19	0.26013994078566327
1106	20-21	0.28535153073296016
1106	22-23	0.3188355039234949
1106	24-25	0.36082917330517006
1106	26-27	0.405997753129391
1106	28-29	0.3804739179878496
1106	30-31	0.41904545548749184
1106	32-33	0.3091393901538666
1106	34-35	0.2865538590969976
1106	36-37	0.32614300177039723
1106	38-39	0.3845734489421204
1106	40-41	0.38575321117626515
1106	42-43	0.2953858348444456
1106	44-45	0.31678578283317904
1106	46-47	0.3429985107956668
1106	48-49	0.22743365254810755
1106	50-51	0.30233562824648885
1106	52-53	0.21725454751805273
1106	54-55	0.2589129438777107
1106	56-57	0.30748373740655666
1106	58-59	0.2794925003667714
1106	60-61	0.2567600300216739
1106	62-63	0.24822957882787478
1106	64-65	0.21665924646463708
1106	66-67	0.19917837871266642
1106	68-69	0.2352278066140272
1106	70-71	0.2957425004334624
1106	72-73	0.19146993629271236
1106	74-75	0.17863733164791284
1106	76-77	0.1418705252100665
1106	78-79	0.1479278694735271
1106	80-81	0.15192901945663806
1106	82-83	0.17987414489656572
1106	84-85	0.17560634607724523
1106	86-87	0.17411917504437469
1106	88-89	0.2835535086837311
1106	90-91	0.20993820630748772
1106	92-93	0.2888858929248279
1106	94-95	0.25883781575625164
1106	96-97	0.28903463547137775
1106	98-99	0.2540954730837832
1106	100	0.350678722048837
1107	1	0.12551666894009372
1107	2	0.1604448787484536
1107	3	0.23005401210350485
1107	4	0.2579919295422286
1107	5	0.1689692870954218
1107	6	0.19449076026702983
1107	7	0.15857709545631593
1107	8	0.1776198390979289
1107	9	0.2510912747249776
1107	10-11	0.3468247380867737
1107	12-13	0.25392598901628816
1107	14-15	0.4157987968918704
1107	16-17	0.30378230246393656
1107	18-19	0.3391409521856872
1107	20-21	0.2948861224644901
1107	22-23	0.39619737110658093
1107	24-25	0.4421547853291443
1107	26-27	0.35227355444068564
1107	28-29	0.4255426457951259
1107	30-31	0.4577920210450799
1107	32-33	0.47752683070066126
1107	34-35	0.4802529196242915
1107	36-37	0.4833485348732296
1107	38-39	0.4840195668189651
1107	40-41	0.45509336060435857
1107	42-43	0.44344670512324313
1107	44-45	0.45764037632051213
1107	46-47	0.4463980229524296
1107	48-49	0.4202841816821561
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2216	100	-1.0258615708560015
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	2.0
2	19.0
3	22.0
4	31.0
5	40.0
6	45.0
7	180.0
8	456.0
9	846.0
10	1365.0
11	3027.0
12	5508.0
13	8285.0
14	10982.0
15	13977.0
16	16591.0
17	19380.0
18	21954.0
19	24726.0
20	27748.0
21	31376.0
22	36019.0
23	41153.0
24	47890.0
25	54884.0
26	61345.0
27	68556.0
28	77670.0
29	93340.0
30	108359.0
31	126256.0
32	156389.0
33	202271.0
34	289412.0
35	405601.0
36	657349.0
37	1089616.0
38	956838.0
39	334080.0
40	477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69976214403749	20.20172878329623	11.486547674573295	24.611961398092987
2	15.938940445146487	19.932446639573765	39.64898045169877	24.47963246358098
3	18.762774951193105	26.339043174553968	28.758711798302606	26.13947007595032
4	12.00275234336685	16.15305544357806	40.39660659132567	31.447585621729413
5	12.894702484316827	38.8465259933743	35.38919975778391	12.869571764524956
6	30.196744493675233	38.47946480723933	18.0764522754483	13.247338423637133
7	27.088796529612193	31.71915072895124	23.707655389721797	17.48439735171477
8	26.51947928683177	34.91079389915532	19.92073683664608	18.648989977366824
9	26.362081597844146	14.589662975525371	21.08031411111149	37.96794131551899
10-11	25.7565879120142	25.70374484910292	27.91847791153963	20.621189327343252
12-13	25.748463246109193	23.58306620709306	27.858384263111528	22.810086283686214
14-15	23.070237433379518	24.938979928829358	25.052124491657928	26.9386581461332
16-17	22.249662772248897	27.493265206636288	26.28651398365067	23.970558037464144
18-19	21.55689845780721	28.357528735022257	27.78533129702024	22.300241510150297
20-21	23.227444473401267	27.424048996145494	26.869516385128588	22.478990145324648
22-23	23.627485727902027	27.158235566208315	26.525447486088332	22.688831219801322
24-25	23.092125168627387	27.106923729860576	27.473413590991907	22.327537510520127
26-27	23.132895337971604	27.347677065677377	26.254110399140867	23.265317197210152
28-29	22.242459327989696	27.887580569067726	26.81913681062234	23.05082329232024
30-31	23.031964183533855	27.649286589300615	26.82563402464159	22.493115202523935
32-33	23.433427561221844	27.65848352874281	25.955953493415613	22.952135416619733
34-35	23.302068722737246	27.442197482377672	26.97841967921326	22.277314115671818
36-37	22.92731813413024	27.070018477507084	26.65494041519621	23.347722973166462
38-39	23.18352431926718	27.295525834765794	27.136706866362175	22.38424297960485
40-41	22.76787073126579	26.377951888680094	27.79497259775816	23.059204782295957
42-43	22.76993201413689	26.575418046218775	27.12710537633547	23.527544563308865
44-45	22.887462940713352	27.492598156664123	27.083256142133017	22.53668276048951
46-47	22.794246059562195	27.3028479301436	26.60702769822355	23.29587831207066
48-49	23.511740869200352	26.990064436209455	26.772196043499573	22.725998651090617
50-51	23.04615901021572	26.668887069484853	26.90690610700232	23.3780478132971
52-53	22.572437726573664	27.340440415288704	26.698305402496576	23.38881645564106
54-55	22.53075719714107	27.217122809884682	26.712812726627106	23.539307266347144
56-57	23.65243422950587	26.63621023847477	26.413319491990546	23.298036040028812
58-59	22.524661136868172	27.081873201966822	27.364231190078286	23.02923447108672
60-61	22.489794393848527	27.704854881472574	26.624077510035377	23.18127321464352
62-63	22.97274686820335	26.970774704192923	26.205860238896587	23.850618188707138
64-65	22.11009935855362	27.23643358575246	26.735210040371644	23.91825701532228
66-67	22.767114412840698	26.82954353721981	26.62808529557105	23.775256754368442
68-69	22.284448371097554	26.85543370648998	26.981660429589375	23.878457492823088
70-71	22.2367496059233	26.562236383139375	27.064656013570875	24.13635799736645
72-73	22.840239079966267	26.5100148328585	27.58665367147792	23.063092415697316
74-75	22.99090692808531	26.73540574574164	27.052619900542147	23.221067425630903
76-77	22.735104250744488	26.694370606988443	26.79904640289848	23.771478739368582
78-79	22.55738882857639	26.67881861731709	26.786850419861107	23.97694213424541
80-81	22.11157267379367	27.075092066385327	27.487084071661737	23.32625118815927
82-83	22.91545014357976	26.07714859003472	28.43477033245623	22.57263093392928
84-85	23.096738043370728	26.50883968097765	27.229745994097133	23.164676281554485
86-87	21.749009203114035	27.23088628872884	27.69690451632761	23.32319999182951
88-89	23.006636310195912	25.949585910224027	27.43443766285013	23.609340116729932
90-91	22.592182013221265	26.025807489112367	27.54485399764549	23.83715650002088
92-93	22.92685383623657	26.18921791775022	27.493793077484103	23.39013516852911
94-95	21.780814415176458	26.294558433492405	28.112515786199022	23.812111365132118
96-97	22.10525757177367	26.12412860595577	27.700382831632382	24.070230990638176
98-99	21.4927622284146	26.78000518664409	27.57459305370179	24.152639531239515
100	22.30521578650148	26.061426836634954	26.716142938619942	24.917214438243626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	232.0
1	176.0
2	151.5
3	182.5
4	175.5
5	163.5
6	149.5
7	154.5
8	167.0
9	183.5
10	193.0
11	167.5
12	194.5
13	222.5
14	239.0
15	354.0
16	448.0
17	531.5
18	687.0
19	877.0
20	1213.5
21	1708.5
22	2491.5
23	3736.5
24	5232.5
25	7236.5
26	10405.0
27	14024.5
28	18777.0
29	27645.0
30	40982.0
31	53881.0
32	70090.5
33	85387.5
34	96936.0
35	112331.5
36	126696.0
37	148527.0
38	158532.5
39	151102.5
40	143495.5
41	134691.5
42	135023.0
43	145100.0
44	159295.0
45	185660.0
46	211495.5
47	230042.0
48	242131.5
49	238223.5
50	226115.0
51	230159.5
52	230896.0
53	217181.0
54	203959.0
55	175382.5
56	153090.5
57	126802.5
58	99745.0
59	96026.5
60	79574.0
61	53845.5
62	39880.5
63	33129.0
64	24481.0
65	12284.0
66	6793.0
67	4699.0
68	3304.5
69	2332.5
70	1822.5
71	1413.0
72	1017.0
73	740.5
74	545.5
75	397.0
76	250.0
77	132.0
78	80.0
79	58.0
80	36.5
81	28.5
82	26.0
83	25.0
84	26.5
85	20.5
86	12.0
87	13.5
88	13.0
89	8.5
90	7.5
91	5.5
92	4.0
93	6.5
94	9.0
95	10.0
96	8.0
97	5.5
98	4.5
99	4.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05624676490994811
2	0.0076691032255287025
3	0.008129649894424681
4	0.010051931642860074
5	0.0035442069736777554
6	0.002522994794821453
7	8.209744967276156E-4
8	0.00136161623847507
9	7.208556556632723E-4
10-11	6.407605828117976E-4
12-13	3.103684072994645E-4
14-15	2.3027333444798978E-4
16-17	7.308675397697066E-4
18-19	0.0014417113113265447
20-21	7.909388444083126E-4
22-23	0.0011013072517077772
24-25	0.0013215687020493327
26-27	0.003534195089571321
28-29	0.004265062629341028
30-31	0.005456476838006714
32-33	0.006417617712224411
34-35	0.0037344327717000075
36-37	0.008530125258682056
38-39	0.006587819742033794
40-41	0.006267439450627895
42-43	0.014967766739119336
44-45	0.021065004159937846
46-47	0.01932293632541827
48-49	0.02287715518320246
50-51	0.019563221543972695
52-53	0.0269419801304148
54-55	0.020143910822145887
56-57	0.01624928790474293
58-59	0.018732235163138645
60-61	0.017090286169683416
62-63	0.011323440924377235
64-65	0.011703892520421741
66-67	0.014467172533797618
68-69	0.005466488722113148
70-71	0.008620232215639964
72-73	0.00845003018583058
74-75	0.011874094550231125
76-77	0.011043108169397076
78-79	0.012574926437681528
80-81	0.010442395123011015
82-83	0.006507724669182319
84-85	0.011193286430993588
86-87	0.009681491930922005
88-89	0.008039542937466773
90-91	0.013405912818515577
92-93	0.008950624391152299
94-95	0.007679115109635136
96-97	0.009731551351454176
98-99	0.009411171060048278
100	0.25674475602540214
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4994065.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.6056477248676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.53460350147171	51.06565795358048
2	14.585670981590457	19.721474684244566
3	4.997746763976623	10.136277214305018
4	2.1921694032355927	5.928121297135149
5	1.0800268368850774	3.6507956933927783
6	0.5707805988203567	2.3152795255222807
7	0.33364787096437226	1.57895362999986
8	0.22125525288589917	1.1966483747104475
9	0.1335894221899655	0.8128259474709076
>10	0.34781805339385097	3.2789543319388588
>50	0.002133238956594293	0.09535558474988266
>100	4.7375773051863137E-4	0.0583068984192138
>500	0.0	0.0
>1k	8.431789894731555E-5	0.16134886453053524
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0023768212868675E-5
2	0.0	0.0	0.0	0.0	2.0023768212868675E-5
3	0.0	0.0	0.0	0.0	2.0023768212868675E-5
4	0.0	0.0	0.0	0.0	2.0023768212868675E-5
5	0.0	0.0	0.0	0.0	2.0023768212868675E-5
6	0.0	0.0	0.0	0.0	6.007130463860602E-5
7	0.0	0.0	0.0	0.0	6.007130463860602E-5
8	0.0	0.0	0.0	0.0	6.007130463860602E-5
9	0.0	0.0	0.0	0.0	6.007130463860602E-5
10-11	0.0	0.0	0.0	0.0	6.007130463860602E-5
12-13	0.0	0.0	0.0	0.0	1.8021391391581808E-4
14-15	0.0	0.0	0.0	0.0	2.3027333444798978E-4
16-17	0.0	0.0	0.0	0.0	2.6030898676729277E-4
18-19	0.0	0.0	0.0	1.0011884106434338E-5	2.8033275498016145E-4
20-21	0.0	0.0	0.0	2.0023768212868675E-5	3.6042782783163616E-4
22-23	0.0	0.0	0.0	5.0059420532171683E-5	4.004753642573735E-4
24-25	0.0	0.0	0.0	2.8033275498016145E-4	4.004753642573735E-4
26-27	0.0	0.0	0.0	6.407605828117976E-4	4.805704371088482E-4
28-29	0.0	0.0	0.0	0.0029034463908659577	4.805704371088482E-4
30-31	0.0	0.0	0.0	0.011713904404528176	4.805704371088482E-4
32-33	0.0	0.0	0.0	0.026831849405244024	4.805704371088482E-4
34-35	0.0	0.0	0.0	0.05354355620121083	5.206179735345855E-4
36-37	0.0	0.0	0.0	0.09859703468016535	5.206179735345855E-4
38-39	0.0	0.0	0.0	0.16658773964696094	5.206179735345855E-4
40-41	0.0	0.0	0.0	0.24055553942529784	5.206179735345855E-4
42-43	0.0	0.0	0.0	0.3198296377800449	5.206179735345855E-4
44-45	0.0	0.0	0.0	0.41581357070843095	5.506536258538886E-4
46-47	0.0	0.0	0.0	0.5351652411412346	5.806892781731916E-4
48-49	0.0	0.0	0.0	0.6740601093497982	5.806892781731916E-4
50-51	0.0	0.0	0.0	0.8208143065819127	6.207368145989289E-4
52-53	0.0	0.0	0.0	0.9667875768537254	6.607843510246662E-4
54-55	0.0	0.0	0.0	1.125646141970519	6.607843510246662E-4
56-57	0.0	0.0	0.0	1.3201570263903253	6.80808119237535E-4
58-59	0.0	0.0	0.0	1.5556865999941931	7.008318874504036E-4
60-61	0.0	0.0	0.0	1.799405894797124	7.008318874504036E-4
62-63	0.0	0.0	0.0	2.0554598308191823	7.208556556632723E-4
64-65	0.0	0.0	0.0	2.3202140941297316	7.408794238761411E-4
66-67	0.0	0.0	0.0	2.626547712134303	7.809269603018783E-4
68-69	0.0	0.0	0.0	2.9686938395875906	8.00950728514747E-4
70-71	0.0	1.0011884106434338E-5	0.0	3.346512310112103	8.409982649404844E-4
72-73	0.0	2.0023768212868675E-5	0.0	3.745876355233662	9.311052218983934E-4
74-75	0.0	2.0023768212868675E-5	0.0	4.135749134222322	0.0010212121788563025
76-77	0.0	2.0023768212868675E-5	0.0	4.582749723922296	0.001041235947069171
78-79	0.0	2.0023768212868675E-5	0.0	5.063169982769548	0.0010512478311756053
80-81	0.0	2.0023768212868675E-5	0.0	5.579823250197984	0.0010612597152820397
82-83	0.0	2.0023768212868675E-5	0.0	6.12158632296536	0.0011413547881335144
84-85	0.0	2.0023768212868675E-5	0.0	6.654278628732305	0.0011613785563463833
86-87	0.0	2.0023768212868675E-5	0.0	7.214473580139626	0.0012014260927721205
88	0.0	2.0023768212868675E-5	0.0	7.664738044058297	0.0012414736291978578
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5395	0.0	40.25717	94
CAACGCA	7430	0.0	33.83742	5
CTGTGCG	6970	0.0	33.23755	9
GTACTAG	5595	0.0	32.69144	1
TAGTACT	7450	0.0	31.983288	4
GCATCGT	5420	0.0	31.471846	8
GTATCAA	9055	0.0	31.20833	1
ATCAACG	8125	0.0	31.003649	3
CTAACGC	2555	0.0	30.166458	3
TAGGCAT	6800	0.0	29.854313	5
TCTAACG	2620	0.0	29.777111	2
ACTGTGC	8245	0.0	29.351576	8
CAGTACG	850	0.0	28.747448	9
GGCGTCG	3945	0.0	28.58769	8
CTAGTAC	8580	0.0	28.537888	3
TAACGCC	2695	0.0	28.424984	4
GTACTGT	8670	0.0	28.021734	6
ATACGGG	4130	0.0	27.879658	3
TACTGTG	9310	0.0	27.710342	7
CGCCTAC	2745	0.0	27.561493	7
>>END_MODULE