FastQCFastQC Report
Mon 16 Jan 2017
ERR1146651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4994065
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90590.18139531624037733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72840.14585312766253541No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC53250.042.47574694
GTATCAA141900.038.619151
GGTATCA82600.038.041321
CTGTGCG70300.034.1683929
CAACGCA153850.033.603175
TAGTACT73850.032.7368554
GTGGTAT19750.032.6296651
GTACTAG50900.032.4375841
ATCAACG160650.032.264523
ACTGTGC82800.031.0489128
TATCAAC182500.029.8730622
TACTGTG91600.029.7580537
GTACTGT84200.029.750016
TACCCCG4500.029.2435825
AACGCAG183900.029.1847766
CTAGTAC86400.029.0704563
GAACCGT6550.028.700586
TAGGCAT66900.028.3115715
CAGTACG9200.027.590849
ACGCAGA191950.026.8590417
Produced by FastQC (version 0.11.5)