##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146651_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4994065 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07886220944261 33.0 31.0 34.0 30.0 34.0 2 32.14267895992543 33.0 31.0 34.0 30.0 34.0 3 32.30673749740942 34.0 31.0 34.0 30.0 34.0 4 35.93094022604832 37.0 35.0 37.0 35.0 37.0 5 35.83567995210315 37.0 35.0 37.0 35.0 37.0 6 35.866679949099584 37.0 35.0 37.0 35.0 37.0 7 35.78533539311162 37.0 35.0 37.0 33.0 37.0 8 35.78632016203233 37.0 35.0 37.0 33.0 37.0 9 37.49247937301577 39.0 37.0 39.0 34.0 39.0 10-11 37.42834124105313 39.0 37.0 39.0 34.0 39.0 12-13 37.401862410681474 39.0 37.0 39.0 34.0 39.0 14-15 38.62072209712929 40.0 38.0 41.0 34.0 41.0 16-17 38.61450812113979 40.0 38.0 41.0 34.0 41.0 18-19 38.66344470886943 40.0 38.0 41.0 34.0 41.0 20-21 38.65880620296292 40.0 38.0 41.0 34.0 41.0 22-23 38.622504612975604 40.0 38.0 41.0 34.0 41.0 24-25 38.55311064233245 40.0 38.0 41.0 34.0 41.0 26-27 38.41438297258846 40.0 38.0 41.0 34.0 41.0 28-29 38.355719338855224 40.0 38.0 41.0 34.0 41.0 30-31 38.233862594900145 40.0 37.5 41.0 33.5 41.0 32-33 38.104733318449 40.0 37.0 41.0 33.0 41.0 34-35 38.01223982867664 40.0 37.0 41.0 33.0 41.0 36-37 38.010432783714265 40.0 37.0 41.0 33.0 41.0 38-39 37.91915093215647 40.0 37.0 41.0 33.0 41.0 40-41 37.815396976210764 40.0 37.0 41.0 33.0 41.0 42-43 37.687718121410114 40.0 37.0 41.0 32.5 41.0 44-45 37.49926923257907 40.0 36.0 41.0 32.0 41.0 46-47 37.28467490911712 40.0 36.0 41.0 31.5 41.0 48-49 37.04341943887394 39.5 35.5 41.0 31.0 41.0 50-51 36.8489616174399 39.0 35.0 41.0 31.0 41.0 52-53 36.66932588983123 39.0 35.0 41.0 30.5 41.0 54-55 36.48886678487364 39.0 35.0 41.0 30.0 41.0 56-57 36.275777948424775 38.5 35.0 40.5 30.0 41.0 58-59 36.01012351661422 38.0 35.0 40.0 30.0 41.0 60-61 35.83490302989649 37.0 35.0 40.0 30.0 41.0 62-63 35.62690763936793 37.0 35.0 40.0 30.0 41.0 64-65 35.38294545625658 36.0 34.0 40.0 29.0 41.0 66-67 35.13158138710649 36.0 34.0 39.0 29.0 41.0 68-69 34.87436937644985 35.5 34.0 39.0 29.0 41.0 70-71 34.64616279523795 35.0 34.0 39.0 29.0 41.0 72-73 34.39972056831459 35.0 34.0 37.5 29.0 40.0 74-75 34.13672599375459 35.0 34.0 37.0 29.0 39.5 76-77 33.88473247745073 35.0 34.0 36.5 29.0 39.0 78-79 33.64977418195398 35.0 33.5 36.0 29.0 39.0 80-81 33.50814977378148 35.0 33.0 36.0 29.0 38.5 82-83 33.34372930668704 35.0 33.0 36.0 29.0 37.0 84-85 33.210236751023466 35.0 33.0 35.0 29.0 37.0 86-87 33.065665144526555 35.0 33.0 35.0 29.0 36.5 88-89 32.95876825792216 35.0 33.0 35.0 29.0 36.0 90-91 32.89479962715744 35.0 33.0 35.0 29.0 36.0 92-93 32.82869776424616 35.0 33.0 35.0 29.0 36.0 94-95 32.78381098363758 35.0 33.0 35.0 29.0 35.5 96-97 32.69620589639902 35.0 33.0 35.0 28.5 35.0 98-99 32.61775147099607 35.0 33.0 35.0 27.5 35.0 100 31.366613770545637 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3811822358915151 1101 2 0.4497614616309207 1101 3 0.41391974329451386 1101 4 0.2843889866493754 1101 5 0.2838568169932856 1101 6 0.27744769789333645 1101 7 0.29485757926678247 1101 8 0.3186534500811433 1101 9 0.4108876290082222 1101 10-11 0.3933411700900393 1101 12-13 0.4211411767812976 1101 14-15 0.5440939962360787 1101 16-17 0.5373031433869855 1101 18-19 0.5596916661799298 1101 20-21 0.5525775050220929 1101 22-23 0.5381925952120099 1101 24-25 0.5306450874449382 1101 26-27 0.5853836070556468 1101 28-29 0.5968007764548631 1101 30-31 0.6516761434039822 1101 32-33 0.7085548560145583 1101 34-35 0.6928602669186361 1101 36-37 0.6030687653930684 1101 38-39 0.6447280903398109 1101 40-41 0.6020936028483348 1101 42-43 0.5929181007369451 1101 44-45 0.5340638018931756 1101 46-47 0.5833475437514437 1101 48-49 0.5750863914859892 1101 50-51 0.5662757493273034 1101 52-53 0.5934219454649252 1101 54-55 0.647973793792076 1101 56-57 0.595506426367379 1101 58-59 0.5033882659302478 1101 60-61 0.5896694494863866 1101 62-63 0.5934530983180935 1101 64-65 0.5813747200364787 1101 66-67 0.505084105106711 1101 68-69 0.5414074839594107 1101 70-71 0.5111419578208967 1101 72-73 0.592935733793297 1101 74-75 0.6162935896818169 1101 76-77 0.5654644263619772 1101 78-79 0.5339540852506843 1101 80-81 0.5684579188991989 1101 82-83 0.49685852189416124 1101 84-85 0.5372342886663901 1101 86-87 0.5309055237078937 1101 88-89 0.5453596642692204 1101 90-91 0.5466845119941013 1101 92-93 0.5264284903308649 1101 94-95 0.5270638693079803 1101 96-97 0.538935547669368 1101 98-99 0.5331441791076372 1101 100 0.874933290191592 1102 1 0.35015963485336243 1102 2 0.4149630666614499 1102 3 0.3438670950614622 1102 4 0.2547882245291717 1102 5 0.22840664767063856 1102 6 0.21753535518161726 1102 7 0.23533074011319854 1102 8 0.27165965072342857 1102 9 0.3400715813959039 1102 10-11 0.32717893678162113 1102 12-13 0.3340873570913985 1102 14-15 0.5072696205081186 1102 16-17 0.44491165462691384 1102 18-19 0.4840081143766213 1102 20-21 0.4633090259635608 1102 22-23 0.4847961445916127 1102 24-25 0.4770175097349352 1102 26-27 0.5091927269145273 1102 28-29 0.5452624314406265 1102 30-31 0.5705461588214362 1102 32-33 0.5251139708240657 1102 34-35 0.5560420177753471 1102 36-37 0.5657123193507729 1102 38-39 0.6120184904529182 1102 40-41 0.5784889156744484 1102 42-43 0.5839848333822175 1102 44-45 0.6258194723502513 1102 46-47 0.6362470341003856 1102 48-49 0.6474313922602306 1102 50-51 0.6436426307610503 1102 52-53 0.6287295538751749 1102 54-55 0.6678661746097987 1102 56-57 0.6071229358299277 1102 58-59 0.6131582833674472 1102 60-61 0.5775260874052321 1102 62-63 0.6291757323455442 1102 64-65 0.6132815353569825 1102 66-67 0.5958643694250796 1102 68-69 0.573750061029827 1102 70-71 0.5609205592091371 1102 72-73 0.5310909248956079 1102 74-75 0.5013477527297567 1102 76-77 0.4780481956253766 1102 78-79 0.4967756804517549 1102 80-81 0.4431194419277489 1102 82-83 0.4147766949600822 1102 84-85 0.42255371263278363 1102 86-87 0.39367019553166216 1102 88-89 0.4242596730214814 1102 90-91 0.432908112136829 1102 92-93 0.44327417445492756 1102 94-95 0.41893574289836977 1102 96-97 0.43024152007954086 1102 98-99 0.4518497192906281 1102 100 0.7590919620018965 1103 1 0.3413007285815155 1103 2 0.3781523904650328 1103 3 0.30664530940580903 1103 4 0.22793794477704665 1103 5 0.2688887240195186 1103 6 0.2980803839395776 1103 7 0.31352896199382485 1103 8 0.23372481005330314 1103 9 0.3413353981934293 1103 10-11 0.34845516199867177 1103 12-13 0.3759623334653739 1103 14-15 0.5003916099853782 1103 16-17 0.47059716320380574 1103 18-19 0.474731236246555 1103 20-21 0.42751046178447183 1103 22-23 0.44656483492797605 1103 24-25 0.4314438171872368 1103 26-27 0.5165779872696064 1103 28-29 0.5577923705345782 1103 30-31 0.5024561003704946 1103 32-33 0.5022158651934774 1103 34-35 0.454473286725424 1103 36-37 0.43999847958509264 1103 38-39 0.45928127485176873 1103 40-41 0.5057645840186709 1103 42-43 0.44641427088990326 1103 44-45 0.4764769903537811 1103 46-47 0.4970190603307145 1103 48-49 0.4793738625822712 1103 50-51 0.5043933184368825 1103 52-53 0.4276453849988542 1103 54-55 0.3885960226325409 1103 56-57 0.3603563780132646 1103 58-59 0.46067293951484345 1103 60-61 0.45388544348980275 1103 62-63 0.43318047478084054 1103 64-65 0.47350114681763245 1103 66-67 0.41845656233255824 1103 68-69 0.4031089633612197 1103 70-71 0.4622715368221577 1103 72-73 0.4744332391222841 1103 74-75 0.3929364585367381 1103 76-77 0.4247611165308669 1103 78-79 0.3733491233483761 1103 80-81 0.3184182651749268 1103 82-83 0.3024233783209169 1103 84-85 0.31525324214269546 1103 86-87 0.3426221953382438 1103 88-89 0.3843004413483655 1103 90-91 0.347736757867267 1103 92-93 0.35936169471987256 1103 94-95 0.32264948956250805 1103 96-97 0.36433208491207836 1103 98-99 0.3836609658509147 1103 100 0.5889441389151386 1104 1 0.23668365908140743 1104 2 0.2622507147642068 1104 3 0.2088821049468379 1104 4 0.190992847092474 1104 5 0.1582772642444823 1104 6 0.24118572721299358 1104 7 0.25123210838220444 1104 8 0.24710064941201892 1104 9 0.2542320928319626 1104 10-11 0.27362674821635835 1104 12-13 0.328275248401134 1104 14-15 0.3138869042766146 1104 16-17 0.3187362210432241 1104 18-19 0.3394908959067635 1104 20-21 0.4464537037110716 1104 22-23 0.4490904043424706 1104 24-25 0.4298384726917419 1104 26-27 0.4123516290486293 1104 28-29 0.44218114745680026 1104 30-31 0.41721786329168964 1104 32-33 0.4484908935167482 1104 34-35 0.4870817593553056 1104 36-37 0.43881819319941684 1104 38-39 0.5073963606616019 1104 40-41 0.4725556046508288 1104 42-43 0.4587609274060327 1104 44-45 0.5190228243983839 1104 46-47 0.4720598648801442 1104 48-49 0.5002827852070908 1104 50-51 0.4579414650394398 1104 52-53 0.39462056960663006 1104 54-55 0.456298482084307 1104 56-57 0.3849589034958427 1104 58-59 0.3749205130141675 1104 60-61 0.3691109690252574 1104 62-63 0.4301853700403768 1104 64-65 0.41693553465870536 1104 66-67 0.38017523464616687 1104 68-69 0.4143267090179279 1104 70-71 0.40611673591349984 1104 72-73 0.35410103130157466 1104 74-75 0.3520049576947315 1104 76-77 0.3348639634200268 1104 78-79 0.2780333235361141 1104 80-81 0.2643917778510314 1104 82-83 0.26930037858964084 1104 84-85 0.2594548539223993 1104 86-87 0.2630632935466437 1104 88-89 0.3055073323774451 1104 90-91 0.2568661551138405 1104 92-93 0.2849076871200964 1104 94-95 0.25080024526353384 1104 96-97 0.24296440697780497 1104 98-99 0.2369975224692169 1104 100 0.48617668483987586 1105 1 0.18386580801590924 1105 2 0.13581924176185822 1105 3 0.1262188876776591 1105 4 0.18215670482847202 1105 5 0.13919137773984147 1105 6 0.14987007413761688 1105 7 0.2233854991852695 1105 8 0.214698673486879 1105 9 0.21707862239714615 1105 10-11 0.22797085866726263 1105 12-13 0.2158984800765964 1105 14-15 0.29935284872734513 1105 16-17 0.3022175976973003 1105 18-19 0.27412905290946554 1105 20-21 0.29696682428762955 1105 22-23 0.29313133118967016 1105 24-25 0.27060172275444927 1105 26-27 0.3035029855217388 1105 28-29 0.3091716381341385 1105 30-31 0.3258234777791813 1105 32-33 0.33595958886023425 1105 34-35 0.32624763949187496 1105 36-37 0.38048871027144315 1105 38-39 0.4217036471146045 1105 40-41 0.4110329114766458 1105 42-43 0.37817652933877355 1105 44-45 0.4160762850375832 1105 46-47 0.44569407577589004 1105 48-49 0.4168439731086977 1105 50-51 0.40163830277335677 1105 52-53 0.4465453228880918 1105 54-55 0.4300717026574432 1105 56-57 0.38468706405707564 1105 58-59 0.4227279741343466 1105 60-61 0.35122769109697316 1105 62-63 0.3956948976568242 1105 64-65 0.3454648293359739 1105 66-67 0.32151360429332243 1105 68-69 0.29041968557722697 1105 70-71 0.33832909141645473 1105 72-73 0.3550368658180645 1105 74-75 0.3121663060773727 1105 76-77 0.31452124034489515 1105 78-79 0.2932691838729653 1105 80-81 0.22788490034243836 1105 82-83 0.29862848428797406 1105 84-85 0.28948394195681004 1105 86-87 0.3046625678707713 1105 88-89 0.2802927217278324 1105 90-91 0.3253321606873243 1105 92-93 0.3005829748412978 1105 94-95 0.2869314238497651 1105 96-97 0.30307502009495835 1105 98-99 0.29538157159567646 1105 100 0.43900000559221297 1106 1 0.1700640766410899 1106 2 0.21515541509587877 1106 3 0.14933710877689066 1106 4 0.12255278309795159 1106 5 0.05987414383446321 1106 6 0.13434537828520376 1106 7 0.15615728718481847 1106 8 0.09725356730358925 1106 9 0.09742211465009376 1106 10-11 0.18861769004465145 1106 12-13 0.15225620252315508 1106 14-15 0.21464322073330067 1106 16-17 0.1581664899416637 1106 18-19 0.2224712993483564 1106 20-21 0.2032730627460282 1106 22-23 0.2416743047763532 1106 24-25 0.2101532564855475 1106 26-27 0.23216163775104093 1106 28-29 0.22950649616333152 1106 30-31 0.2753659065724392 1106 32-33 0.2817356351054485 1106 34-35 0.31329959131470986 1106 36-37 0.26214365787151195 1106 38-39 0.23316653150859423 1106 40-41 0.22873837013256093 1106 42-43 0.22677625346058505 1106 44-45 0.27570759502966524 1106 46-47 0.2366914601176191 1106 48-49 0.3397600519922577 1106 50-51 0.36548029275236615 1106 52-53 0.3499549374135924 1106 54-55 0.2664799625826504 1106 56-57 0.20769047372395022 1106 58-59 0.23375808488489014 1106 60-61 0.23441600871537815 1106 62-63 0.28974417691070187 1106 64-65 0.2528899337888504 1106 66-67 0.29054318846615246 1106 68-69 0.28507108314307317 1106 70-71 0.3244304532984188 1106 72-73 0.2210402695875473 1106 74-75 0.20333024036704472 1106 76-77 0.2368757959649983 1106 78-79 0.29766124507423797 1106 80-81 0.24963373146017886 1106 82-83 0.20484022577598182 1106 84-85 0.23908200339696606 1106 86-87 0.16794663923694486 1106 88-89 0.18565569965026185 1106 90-91 0.13095511075400879 1106 92-93 0.1911366437851072 1106 94-95 0.21621118155318442 1106 96-97 0.17478649819402392 1106 98-99 0.19242856205810455 1106 100 0.3395407817124436 1107 1 0.14844784628798635 1107 2 0.16195495148979688 1107 3 0.16824292836245291 1107 4 0.16329871550496478 1107 5 0.0811250340528673 1107 6 0.06473495045327127 1107 7 0.1439782591942773 1107 8 0.12516777558134606 1107 9 0.1620340634532127 1107 10-11 0.18988802743183442 1107 12-13 0.06822063370361064 1107 14-15 0.16860611293226668 1107 16-17 0.23669470191428843 1107 18-19 0.2207344686171524 1107 20-21 0.17388460937950612 1107 22-23 0.14216716481249136 1107 24-25 0.16728082856899107 1107 26-27 0.21196720634292632 1107 28-29 0.24111753918507617 1107 30-31 0.16852768879508062 1107 32-33 0.22587043031217746 1107 34-35 0.2323185440172182 1107 36-37 0.12291952535541384 1107 38-39 0.21557751953204018 1107 40-41 0.2620903702430937 1107 42-43 0.24981179003985687 1107 44-45 0.26245068900581003 1107 46-47 0.3318958124641114 1107 48-49 0.23548202978404476 1107 50-51 0.2592492831221733 1107 52-53 0.22511054868792257 1107 54-55 0.2955318906536135 1107 56-57 0.2014841651214283 1107 58-59 0.20140958569064082 1107 60-61 0.2366609744014525 1107 62-63 0.29062766414575947 1107 64-65 0.17024299094423156 1107 66-67 0.2319015323645175 1107 68-69 0.1659492257248374 1107 70-71 0.2192650231881217 1107 72-73 0.22202268144224035 1107 74-75 0.2656573152207642 1107 76-77 0.22129320803851016 1107 78-79 0.1858449192285434 1107 80-81 0.13736377205832184 1107 82-83 0.24668198049023005 1107 84-85 0.18291768885262627 1107 86-87 0.16971106320998786 1107 88-89 0.1519575830545108 1107 90-91 0.12852241527660624 1107 92-93 0.11610212581989288 1107 94-95 0.12573440252493384 1107 96-97 0.10221756803907311 1107 98-99 0.1440649056806862 1107 100 0.18292405025682257 1108 1 0.11506880425667987 1108 2 0.09039825135662483 1108 3 0.11785017193405167 1108 4 0.0764341996386051 1108 5 0.11154235989287997 1108 6 0.10788788461233878 1108 7 0.10570923523369657 1108 8 0.13094402083494572 1108 9 0.09077317095742643 1108 10-11 0.07700008953381143 1108 12-13 0.10965174902403163 1108 14-15 0.2005474915150387 1108 16-17 0.153826721650411 1108 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-0.6328738310209161 2215 10-11 -0.6237659452263102 2215 12-13 -0.6412192526102345 2215 14-15 -0.7331388274906203 2215 16-17 -0.8188533250267866 2215 18-19 -0.7502881372400338 2215 20-21 -0.7316246076625887 2215 22-23 -0.7128810672235772 2215 24-25 -0.8050197472491192 2215 26-27 -0.8265454111457586 2215 28-29 -0.7919381613274297 2215 30-31 -0.7678260414987577 2215 32-33 -0.7051901407417489 2215 34-35 -0.6875863835091636 2215 36-37 -0.759496383878016 2215 38-39 -0.9767019499309484 2215 40-41 -0.9713292278553567 2215 42-43 -0.9614684128437219 2215 44-45 -0.9504175132101906 2215 46-47 -1.0049206301105116 2215 48-49 -0.933803714637861 2215 50-51 -0.9718230792710614 2215 52-53 -0.932310578373162 2215 54-55 -0.9216637081969878 2215 56-57 -0.9961044650974173 2215 58-59 -0.9693802567817258 2215 60-61 -0.8828244609387212 2215 62-63 -0.87451500332579 2215 64-65 -0.8469710576542582 2215 66-67 -0.9454613861597991 2215 68-69 -0.8186352960692318 2215 70-71 -0.9076554276400088 2215 72-73 -0.8623910223911437 2215 74-75 -0.9186572041049885 2215 76-77 -0.8053448444802811 2215 78-79 -0.8752360318894716 2215 80-81 -0.9714009498475136 2215 82-83 -0.871773312812131 2215 84-85 -0.823460106476638 2215 86-87 -0.8037490535715719 2215 88-89 -0.871238925642281 2215 90-91 -0.8329157181245108 2215 92-93 -0.7430788355195332 2215 94-95 -0.7718207898594258 2215 96-97 -0.7381153232316464 2215 98-99 -0.7737497704865959 2215 100 -0.3442160053761789 2216 1 -0.4957405310312488 2216 2 -0.29798966612020905 2216 3 -0.36095292157814995 2216 4 -0.44716906490867814 2216 5 -0.4849259917895239 2216 6 -0.4866151961695522 2216 7 -0.45462827021906094 2216 8 -0.5317850403573416 2216 9 -0.681628523507932 2216 10-11 -0.7155131387642655 2216 12-13 -0.6316120959719811 2216 14-15 -0.929162507020429 2216 16-17 -0.8326770315931924 2216 18-19 -0.7160812495929889 2216 20-21 -0.7821681497235673 2216 22-23 -0.8204119183925087 2216 24-25 -0.991159704359859 2216 26-27 -1.0026919360199003 2216 28-29 -0.8937156490615621 2216 30-31 -0.8818460801182368 2216 32-33 -0.7811007086410058 2216 34-35 -0.8199466958883264 2216 36-37 -1.0225583552340467 2216 38-39 -1.0927656371539172 2216 40-41 -1.276623750869014 2216 42-43 -1.1016034987846552 2216 44-45 -1.1649667871374092 2216 46-47 -1.0839620385581412 2216 48-49 -1.1715291201295202 2216 50-51 -1.1119939489423984 2216 52-53 -1.1392123771694003 2216 54-55 -1.0772751564568779 2216 56-57 -1.025150139289181 2216 58-59 -1.150745478203497 2216 60-61 -1.1702367554198219 2216 62-63 -1.3000613251963316 2216 64-65 -1.1253493416875813 2216 66-67 -1.1498394548168491 2216 68-69 -1.0713089337570096 2216 70-71 -1.142452025773082 2216 72-73 -1.0920345611770017 2216 74-75 -1.012648664260439 2216 76-77 -1.1097145838170306 2216 78-79 -1.0285720122754114 2216 80-81 -1.0394181389768562 2216 82-83 -1.05188873185309 2216 84-85 -0.9873272858951836 2216 86-87 -1.0018119340095595 2216 88-89 -0.9247655908560333 2216 90-91 -0.9774344121248255 2216 92-93 -1.0195780356756643 2216 94-95 -1.0059928237487057 2216 96-97 -1.0089340752002585 2216 98-99 -0.9781929372294798 2216 100 -0.36340322814492865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 0.0 11 6.0 12 19.0 13 59.0 14 249.0 15 670.0 16 1671.0 17 3647.0 18 6222.0 19 9885.0 20 14171.0 21 20249.0 22 27751.0 23 32931.0 24 34631.0 25 35506.0 26 39447.0 27 47922.0 28 61299.0 29 78197.0 30 97594.0 31 121852.0 32 152896.0 33 196859.0 34 276739.0 35 396583.0 36 642477.0 37 1187078.0 38 1103284.0 39 403420.0 40 747.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.82781561713754 21.457550111982922 12.69374747825669 26.020886792622843 2 14.457340109264493 20.928563962101457 38.87055709992923 25.74353882870482 3 17.284576792652878 26.7007938422908 29.518238148682485 26.496391216373837 4 11.614537114749945 17.074051539557296 38.98547779544021 32.32593355025255 5 12.413921537412556 38.92459617789499 35.50472631809758 13.15675596659488 6 30.252682034647943 38.90597695703879 17.449179155329038 13.392161852984232 7 26.29787557831146 32.31924294137141 23.805917624219948 17.576963856097187 8 26.719055518900937 34.701911168557075 19.586449115099626 18.992584197442362 9 26.262173199587913 14.030233887624611 21.111839753787745 38.595753158999734 10-11 25.62339496982919 25.834405439256397 27.743942059224295 20.79825753169012 12-13 25.786796287582227 23.60142569657893 28.222314554319638 22.389463461519206 14-15 23.00382560021131 24.74314349249954 25.71504840550261 26.537982501786537 16-17 21.743529569599115 27.15640465232231 27.038624847694216 24.061440930384368 18-19 21.153319566985797 27.40027782992303 29.399742193855204 22.04666040923597 20-21 22.607535144216186 26.61551261347219 28.712301501882738 22.06465074042889 22-23 22.89501638444834 26.30163003485137 28.396376498904196 22.406977081796093 24-25 22.437893779916763 26.296103474824616 29.3117830865237 21.95421965873492 26-27 22.482927234627503 26.524865014772537 28.173301709128733 22.818906041471227 28-29 21.74854552353644 27.0379840871114 28.65226023289645 22.561210156455715 30-31 22.585879438893965 26.742273078143757 28.581706485598406 22.09014099736387 32-33 23.057959798280557 26.834512566416336 27.89404022574796 22.213487409555142 34-35 22.77408283632672 26.671208724756283 28.65313126681371 21.901577172103288 36-37 22.572133122015835 26.205245626558728 28.326914046973755 22.895707204451686 38-39 22.70023517915766 26.338343613869664 28.959444861050066 22.00197634592261 40-41 22.21508931101217 25.673985020218996 29.41969117342285 22.691234495345974 42-43 22.46965147630237 25.807683720576325 28.589065220416632 23.13359958270467 44-45 22.611680064236246 26.732691705053902 28.33519387512978 22.320434355580073 46-47 22.61049866191169 26.60223685514706 27.684741788502954 23.1025226944383 48-49 23.46883751012452 26.460448552431735 27.634081654924397 22.43663228251935 50-51 22.792614833807733 26.278142154737672 27.571427284186328 23.357815727268267 52-53 22.229075913108858 26.937935329235803 27.31692519020077 23.516063567454566 54-55 22.347186109912467 26.87894530808069 27.177239383147793 23.596629198859045 56-57 23.483284658890103 26.230045063490365 26.961953839207137 23.324716438412395 58-59 22.234260961465644 26.755879931831057 27.88822447774487 23.121634628958425 60-61 22.358529574605058 27.346119844255128 27.07528836729198 23.220062213847836 62-63 22.848190802482545 26.52929026754758 26.67610453608433 23.946414393885544 64-65 21.97420315592893 26.821180880768264 27.145135706705997 24.05948025659681 66-67 22.58253159705626 26.45763754798954 27.07481112049782 23.885019734456375 68-69 22.187196201891645 26.34998743508545 27.491982983801773 23.970833379221133 70-71 22.167875240136144 26.037185910731225 27.51167290943889 24.283265939693738 72-73 22.665957421885206 25.945423594042865 27.958621514085426 23.429997469986503 74-75 22.767727256071076 26.35351649674102 27.427815557202408 23.450940689985497 76-77 22.51718591342797 26.329884636971794 27.036183578854967 24.11674587074527 78-79 22.243742219511446 26.39853562119661 27.147885399122917 24.209836760169022 80-81 21.894749067142698 26.846987374012954 27.68337016038037 23.574893398463978 82-83 22.679971125726237 25.768397087342677 28.640746566174048 22.910885220757038 84-85 22.93952922118555 26.280184579095383 27.243077533031705 23.53720866668736 86-87 21.560782649004366 26.91731084797655 27.766318620202178 23.755587882816904 88-89 22.532055549937777 25.82643597950768 27.512307108537836 24.129201362016715 90-91 22.133963014097734 25.759796878895248 27.687434985327585 24.418805121679434 92-93 22.55933793412781 25.953466765050116 27.414010430380863 24.073184870441214 94-95 21.386105306999408 26.00741079661558 28.03372603280094 24.572757863584073 96-97 21.731094809538924 25.88570633341777 27.613126781489626 24.77007207555368 98-99 21.09697210588969 26.53055176494499 27.526854376144485 24.845621753020836 100 21.861918587469415 25.870935439268987 26.647257447708412 25.619888525553193 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 194.0 1 130.0 2 102.5 3 138.0 4 102.5 5 80.0 6 173.0 7 409.0 8 652.5 9 702.5 10 596.5 11 525.0 12 557.0 13 678.5 14 837.0 15 1165.0 16 1541.0 17 1950.5 18 2637.5 19 3422.0 20 4367.0 21 5534.0 22 6963.5 23 8614.0 24 10253.5 25 12326.5 26 15218.5 27 18237.5 28 22559.0 29 31134.0 30 43594.5 31 55221.0 32 70565.5 33 85389.5 34 95726.0 35 110317.0 36 124276.0 37 145904.5 38 156010.5 39 147889.0 40 140403.0 41 132331.5 42 132962.0 43 141856.5 44 154895.5 45 183370.5 46 210279.5 47 227906.5 48 240953.0 49 236930.0 50 225404.5 51 226900.0 52 226438.5 53 214550.0 54 204375.0 55 176944.0 56 152815.5 57 127125.5 58 99741.0 59 97076.0 60 80155.5 61 52445.5 62 37653.0 63 30948.0 64 22485.5 65 10641.0 66 5770.5 67 4033.0 68 2838.0 69 2011.5 70 1576.0 71 1196.0 72 805.5 73 596.0 74 435.0 75 316.5 76 171.0 77 60.0 78 26.5 79 16.5 80 9.0 81 6.5 82 6.5 83 3.0 84 2.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.006227391914202159 3 0.0 4 0.08387956504370689 5 0.004405229006831109 6 4.004753642573735E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.0035652319303013E-4 14-15 0.009401159175941844 16-17 0.0 18-19 5.0059420532171683E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.004753642573735E-5 60-61 0.0 62-63 0.0 64-65 9.010695695790904E-5 66-67 1.601901457029494E-4 68-69 0.0 70-71 4.6054666889597956E-4 72-73 4.104872483638078E-4 74-75 3.6042782783163616E-4 76-77 8.00950728514747E-5 78-79 4.004753642573735E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.05939049651936849 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4994065.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.54689895836661 #Duplication Level Percentage of deduplicated Percentage of total 1 73.11113599281416 47.190971076595766 2 15.837970108902374 20.445837126499043 3 5.652984291198777 10.946478175717237 4 2.4075301391065627 6.215944185125346 5 1.2008085716770769 3.875423477219041 6 0.6386377174990774 2.4733250533448903 7 0.381102542962574 1.7219291133367287 8 0.22756805893811363 1.1751050005144033 9 0.14949805911048514 0.8684672504288764 >10 0.3843188809682567 3.486165322318753 >50 0.004816265964188622 0.2150067988476118 >100 0.002873859255242324 0.41332767250919944 >500 4.44418591395353E-4 0.2121891723271108 >1k 2.2220929411775035E-4 0.2910178641485258 >5k 8.888371764709989E-5 0.468812711067457 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9059 0.18139531624037733 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7284 0.14585312766253541 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.004753642573735E-5 2 0.0 0.0 0.0 0.0 4.004753642573735E-5 3 0.0 0.0 0.0 0.0 4.004753642573735E-5 4 0.0 0.0 0.0 0.0 4.004753642573735E-5 5 0.0 0.0 0.0 0.0 6.007130463860602E-5 6 0.0 0.0 0.0 0.0 6.007130463860602E-5 7 0.0 0.0 0.0 0.0 6.007130463860602E-5 8 0.0 0.0 0.0 0.0 6.007130463860602E-5 9 0.0 0.0 0.0 0.0 6.007130463860602E-5 10-11 0.0 0.0 0.0 0.0 6.007130463860602E-5 12-13 0.0 0.0 0.0 0.0 1.8021391391581808E-4 14-15 0.0 0.0 0.0 0.0 1.9022579802225242E-4 16-17 0.0 0.0 0.0 0.0 2.0023768212868676E-4 18-19 0.0 0.0 0.0 1.0011884106434338E-5 2.3027333444798978E-4 20-21 0.0 0.0 0.0 2.0023768212868675E-5 2.6030898676729277E-4 22-23 0.0 0.0 0.0 5.0059420532171683E-5 2.6030898676729277E-4 24-25 0.0 0.0 0.0 2.8033275498016145E-4 2.6030898676729277E-4 26-27 0.0 0.0 0.0 6.307486987053633E-4 2.8033275498016145E-4 28-29 0.0 0.0 0.0 0.0028934345067595235 2.8033275498016145E-4 30-31 0.0 0.0 0.0 0.011713904404528176 2.903446390865958E-4 32-33 0.0 0.0 0.0 0.02693196824630837 3.404040596187675E-4 34-35 0.0 0.0 0.0 0.053793853303871694 3.6042782783163616E-4 36-37 0.0 0.0 0.0 0.09925781903119002 3.6042782783163616E-4 38-39 0.0 0.0 0.0 0.16763898747813655 4.405229006831109E-4 40-41 0.0 0.0 0.0 0.24199725073662437 4.405229006831109E-4 42-43 0.0 0.0 0.0 0.32168183633973524 4.405229006831109E-4 44-45 0.0 0.0 0.0 0.4181163040529108 4.405229006831109E-4 46-47 0.0 0.0 0.0 0.538148782604952 4.405229006831109E-4 48-49 0.0 0.0 0.0 0.6774341142936666 4.605466688959795E-4 50-51 0.0 0.0 0.0 0.8255399158801497 5.606655099603229E-4 52-53 0.0 0.0 0.0 0.9726945884765216 5.806892781731916E-4 54-55 0.0 0.0 0.0 1.133044924325174 5.806892781731916E-4 56-57 0.0 0.0 0.0 1.3292978765794998 5.907011622796259E-4 58-59 0.0 0.0 0.0 1.5666095655543129 6.007130463860603E-4 60-61 0.0 0.0 0.0 1.8127016768904691 6.007130463860603E-4 62-63 0.0 0.0 0.0 2.0715489285782223 6.407605828117976E-4 64-65 0.0 0.0 0.0 2.338645972769678 6.607843510246662E-4 66-67 0.0 0.0 0.0 2.6475426331054965 6.607843510246662E-4 68-69 0.0 0.0 0.0 2.9926322544860753 6.607843510246662E-4 70-71 0.0 0.0 0.0 3.3737246111133916 6.80808119237535E-4 72-73 0.0 0.0 0.0 3.7755916272615595 7.408794238761411E-4 74-75 0.0 0.0 0.0 4.169619338154389 8.3098638083405E-4 76-77 0.0 0.0 0.0 4.620464491351234 8.61022033153353E-4 78-79 0.0 0.0 0.0 5.105219896016571 8.61022033153353E-4 80-81 0.0 0.0 0.0 5.627790186951913 8.810458013662217E-4 82-83 0.0 0.0 0.0 6.176321293375237 9.411171060048277E-4 84-85 0.0 0.0 0.0 6.715130860331213 9.611408742176964E-4 86-87 0.0 0.0 0.0 7.283365354676 0.001011200294749868 88 0.0 0.0 0.0 7.740407864134728 0.0010612597152820397 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 5325 0.0 42.475746 94 GTATCAA 14190 0.0 38.61915 1 GGTATCA 8260 0.0 38.04132 1 CTGTGCG 7030 0.0 34.168392 9 CAACGCA 15385 0.0 33.60317 5 TAGTACT 7385 0.0 32.736855 4 GTGGTAT 1975 0.0 32.629665 1 GTACTAG 5090 0.0 32.437584 1 ATCAACG 16065 0.0 32.26452 3 ACTGTGC 8280 0.0 31.048912 8 TATCAAC 18250 0.0 29.873062 2 TACTGTG 9160 0.0 29.758053 7 GTACTGT 8420 0.0 29.75001 6 TACCCCG 450 0.0 29.243582 5 AACGCAG 18390 0.0 29.184776 6 CTAGTAC 8640 0.0 29.070456 3 GAACCGT 655 0.0 28.70058 6 TAGGCAT 6690 0.0 28.311571 5 CAGTACG 920 0.0 27.59084 9 ACGCAGA 19195 0.0 26.859041 7 >>END_MODULE