FastQCFastQC Report
Mon 16 Jan 2017
ERR1146650_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146650_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4895424
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC58400.042.93229794
GTACTAG57400.037.5991021
GCATCGT58700.034.02498
CTGTGCG80950.033.4965829
TAGTACT83000.032.7845044
TAGGCAT72550.032.324135
ACTGTGC89100.031.013138
TACTGTG96600.030.1133827
CTAACGC27650.029.5748313
GTACTGT92300.029.5306036
TCTAACG28550.029.136962
CTAGGCA82950.028.7816374
TAACGCC29200.028.6487274
CTAGTAC100200.028.2824973
CGCCTAC29250.027.9556377
CAGTACG6900.027.9239169
TATTCTC28100.027.0939565
GTACATG198950.027.0370181
TACATGG199150.026.431072
CAACGCA102350.026.3565035
Produced by FastQC (version 0.11.5)