##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146650_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4895424 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.962339115059287 31.0 31.0 34.0 27.0 34.0 2 31.0966666012995 31.0 31.0 34.0 27.0 34.0 3 31.234990268462955 31.0 31.0 34.0 27.0 34.0 4 34.686538898367125 37.0 35.0 37.0 30.0 37.0 5 34.836935064255925 37.0 35.0 37.0 32.0 37.0 6 34.91617273600816 37.0 35.0 37.0 31.0 37.0 7 34.83265821305775 37.0 35.0 37.0 31.0 37.0 8 34.80842108875554 37.0 35.0 37.0 31.0 37.0 9 36.25852489998823 39.0 37.0 39.0 30.0 39.0 10-11 36.09380617490946 39.0 36.0 39.0 30.0 39.0 12-13 35.98816884911297 39.0 35.0 39.0 30.0 39.0 14-15 37.095131494228085 40.0 36.5 41.0 30.0 41.0 16-17 37.11036061432064 40.0 36.0 41.0 30.0 41.0 18-19 37.068560455641844 40.0 36.0 41.0 30.0 41.0 20-21 37.12569289197422 40.0 36.0 41.0 30.0 41.0 22-23 37.05977010367233 40.0 36.0 41.0 30.0 41.0 24-25 36.99713416039142 40.0 36.0 41.0 30.0 41.0 26-27 37.02931043358042 40.0 36.0 41.0 30.0 41.0 28-29 37.00765643588788 40.0 36.0 41.0 30.0 41.0 30-31 36.86137942290596 40.0 36.0 41.0 30.0 41.0 32-33 36.83652417032722 40.0 36.0 41.0 30.0 41.0 34-35 36.76254569573545 40.0 35.0 41.0 30.0 41.0 36-37 36.64458083712463 39.5 35.0 41.0 29.0 41.0 38-39 36.534655220875656 39.0 35.0 41.0 29.0 41.0 40-41 36.4469022499379 39.0 35.0 41.0 29.0 41.0 42-43 36.28726480076088 39.0 35.0 41.0 28.0 41.0 44-45 36.129833289210495 39.0 35.0 41.0 28.0 41.0 46-47 36.12571045940045 39.0 35.0 41.0 28.0 41.0 48-49 35.97840432207711 39.0 34.5 40.5 28.0 41.0 50-51 35.794472654462616 38.0 34.0 40.0 27.5 41.0 52-53 35.57627276003059 38.0 34.0 40.0 27.0 41.0 54-55 35.35305052228367 38.0 34.0 40.0 27.0 41.0 56-57 35.09336780634323 37.0 33.5 40.0 26.5 41.0 58-59 34.78142618494333 37.0 33.0 40.0 26.0 41.0 60-61 34.531103026009596 36.0 33.0 40.0 26.0 41.0 62-63 34.219756041560444 35.5 33.0 39.0 26.0 41.0 64-65 34.05934429785857 35.0 33.0 39.0 26.0 41.0 66-67 33.798497739930184 35.0 32.5 39.0 25.5 41.0 68-69 33.67624142464473 35.0 32.5 38.5 25.5 40.5 70-71 33.612784817004616 35.0 33.0 37.5 26.0 40.0 72-73 33.398756267077175 35.0 32.5 37.0 25.5 40.0 74-75 33.15598618219791 35.0 32.0 37.0 25.0 39.5 76-77 32.90373571318848 35.0 32.0 36.0 25.0 39.0 78-79 32.66104815435803 35.0 32.0 36.0 25.0 39.0 80-81 32.48244758778811 35.0 32.0 35.5 24.5 38.0 82-83 32.22406057166856 35.0 32.0 35.0 24.0 37.0 84-85 32.05746550247741 35.0 32.0 35.0 24.0 37.0 86-87 31.888413342746205 35.0 31.0 35.0 24.0 36.5 88-89 31.761796016034566 35.0 31.0 35.0 24.0 36.0 90-91 31.611407510360696 35.0 31.0 35.0 23.0 36.0 92-93 31.454923720601116 35.0 31.0 35.0 23.0 36.0 94-95 31.380058499529355 35.0 31.0 35.0 23.0 35.0 96-97 31.27572412522388 34.5 31.0 35.0 23.0 35.0 98-99 31.17920633636637 34.0 31.0 35.0 22.5 35.0 100 30.16695244375155 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3494904575923812 1101 2 0.46773498034340477 1101 3 0.5300783585440989 1101 4 0.6269257172153146 1101 5 0.48861745781763943 1101 6 0.4227679980303378 1101 7 0.4365715462857125 1101 8 0.472433043762166 1101 9 0.5907782533776853 1101 10-11 0.6845452081124037 1101 12-13 0.5793127515101375 1101 14-15 0.7587960443601389 1101 16-17 0.7430440840662342 1101 18-19 0.7603746619380587 1101 20-21 0.7201135379935408 1101 22-23 0.6939547789725964 1101 24-25 0.7842842930520604 1101 26-27 0.7387895152457489 1101 28-29 0.7255441354693488 1101 30-31 0.7012466941640838 1101 32-33 0.7022412257229718 1101 34-35 0.737950879074063 1101 36-37 0.7707691251352458 1101 38-39 0.7289607278785724 1101 40-41 0.763860103805797 1101 42-43 0.7290562733448027 1101 44-45 0.7421184780216166 1101 46-47 0.7373052593199958 1101 48-49 0.6899202568216154 1101 50-51 0.6261551072046245 1101 52-53 0.6364886377505456 1101 54-55 0.6364104520934646 1101 56-57 0.5999419960625048 1101 58-59 0.5648340660463447 1101 60-61 0.589091916607444 1101 62-63 0.545258675261131 1101 64-65 0.5365246130363559 1101 66-67 0.6283712794629039 1101 68-69 0.6575586752881861 1101 70-71 0.6125595259240768 1101 72-73 0.5874315385705415 1101 74-75 0.518155119538136 1101 76-77 0.552277717946545 1101 78-79 0.5484627398635027 1101 80-81 0.5025088028092242 1101 82-83 0.5531899753218354 1101 84-85 0.5507776349842288 1101 86-87 0.540759951136117 1101 88-89 0.6252965178703356 1101 90-91 0.6164015121368251 1101 92-93 0.5691138558612643 1101 94-95 0.5593017950226447 1101 96-97 0.5278505914364402 1101 98-99 0.5279699895549612 1101 100 0.6681859262447354 1102 1 0.37690475535551826 1102 2 0.4803722022829504 1102 3 0.40813246043541085 1102 4 0.5343981973622149 1102 5 0.43813306721697387 1102 6 0.432522577552362 1102 7 0.42051971628039553 1102 8 0.5143068879615882 1102 9 0.6068065164989704 1102 10-11 0.7246731842868925 1102 12-13 0.6262220905706428 1102 14-15 0.745810483409457 1102 16-17 0.7638356397415151 1102 18-19 0.8058113105723805 1102 20-21 0.7273914042830327 1102 22-23 0.8311744961341745 1102 24-25 0.8690129010364842 1102 26-27 0.8238360683155506 1102 28-29 0.8017000095903413 1102 30-31 0.8537649804032981 1102 32-33 0.7444280745958665 1102 34-35 0.7815414284785618 1102 36-37 0.8538569727579812 1102 38-39 0.85821830771755 1102 40-41 0.7380078076046672 1102 42-43 0.7566073038028094 1102 44-45 0.7142316434487341 1102 46-47 0.6705953568542782 1102 48-49 0.6619056110744097 1102 50-51 0.6848022414331467 1102 52-53 0.6428640074980123 1102 54-55 0.6109388234241564 1102 56-57 0.6472399009185494 1102 58-59 0.6326280931426993 1102 60-61 0.5938134315033778 1102 62-63 0.6415592788291775 1102 64-65 0.6105502422945435 1102 66-67 0.570111256086598 1102 68-69 0.572399308152086 1102 70-71 0.5550346646201447 1102 72-73 0.5562485219224058 1102 74-75 0.5495558980319402 1102 76-77 0.5274654823784957 1102 78-79 0.5303682014201314 1102 80-81 0.5278517729196324 1102 82-83 0.5568347202456536 1102 84-85 0.5909660312772758 1102 86-87 0.47738003019805575 1102 88-89 0.48581767344963467 1102 90-91 0.5097093356772078 1102 92-93 0.5867788662588289 1102 94-95 0.6576582974489149 1102 96-97 0.5873145404352726 1102 98-99 0.5319988874792045 1102 100 0.680741799265725 1103 1 0.26181994236662476 1103 2 0.2780826691011633 1103 3 0.29201686475800415 1103 4 0.3840196059441894 1103 5 0.23402811041567873 1103 6 0.3198147794123898 1103 7 0.3302249824647632 1103 8 0.3594789589527565 1103 9 0.4575969761864087 1103 10-11 0.5055824532330959 1103 12-13 0.44192248351099295 1103 14-15 0.5667749975757914 1103 16-17 0.5418414927574844 1103 18-19 0.5880852033027821 1103 20-21 0.5073071706460652 1103 22-23 0.584392904111688 1103 24-25 0.6055841449345536 1103 26-27 0.48608339287847 1103 28-29 0.5100969499181645 1103 30-31 0.5313068802605585 1103 32-33 0.44425707350508503 1103 34-35 0.4644280351586758 1103 36-37 0.5006349463941078 1103 38-39 0.557728252964452 1103 40-41 0.4902745042573571 1103 42-43 0.4750411021434857 1103 44-45 0.3884658113443251 1103 46-47 0.3640714141305281 1103 48-49 0.4926870172247817 1103 50-51 0.3424451535773727 1103 52-53 0.32724977600273775 1103 54-55 0.39923585464698164 1103 56-57 0.3916323563400752 1103 58-59 0.45198423930317233 1103 60-61 0.4022626743915225 1103 62-63 0.33924797223617276 1103 64-65 0.3549481316049139 1103 66-67 0.4294993424722193 1103 68-69 0.33222276969984676 1103 70-71 0.3497046221992193 1103 72-73 0.24152092915851142 1103 74-75 0.301050856282167 1103 76-77 0.2790801360293571 1103 78-79 0.28997967032315586 1103 80-81 0.2774384819019673 1103 82-83 0.290246019762769 1103 84-85 0.27930284424484597 1103 86-87 0.29216965920240767 1103 88-89 0.365899861925417 1103 90-91 0.4624874141108961 1103 92-93 0.3892570224200007 1103 94-95 0.31846811786937934 1103 96-97 0.383120377949286 1103 98-99 0.38244408622909276 1103 100 0.5050998677136711 1104 1 0.2077739895002928 1104 2 0.3256525081119044 1104 3 0.27586613141441063 1104 4 0.45291214498814014 1104 5 0.31745991318856426 1104 6 0.3193806978528215 1104 7 0.3182805384264995 1104 8 0.40113935802445866 1104 9 0.3915155114611437 1104 10-11 0.5090625033977574 1104 12-13 0.4616164107291567 1104 14-15 0.5487670671597868 1104 16-17 0.607609165005023 1104 18-19 0.5251140143971327 1104 20-21 0.49206061403404533 1104 22-23 0.5632679448323827 1104 24-25 0.5932224822921199 1104 26-27 0.5119923608119805 1104 28-29 0.4782637781154335 1104 30-31 0.4943639420960011 1104 32-33 0.5809262012984675 1104 34-35 0.5237579892029203 1104 36-37 0.540498493403021 1104 38-39 0.5111777461960898 1104 40-41 0.5331423302431659 1104 42-43 0.5593484971697009 1104 44-45 0.5648525370711113 1104 46-47 0.5223206673531706 1104 48-49 0.4901343274072971 1104 50-51 0.4379323028182682 1104 52-53 0.4788803703314244 1104 54-55 0.5682458746386629 1104 56-57 0.5178333780826634 1104 58-59 0.4661131632558977 1104 60-61 0.5359776875522471 1104 62-63 0.5853878137614927 1104 64-65 0.5044707307245488 1104 66-67 0.5437209049768441 1104 68-69 0.49933773941154413 1104 70-71 0.5154406502658517 1104 72-73 0.5573299333351898 1104 74-75 0.48863558280614683 1104 76-77 0.39111725006559084 1104 78-79 0.38810621405990275 1104 80-81 0.4095796585331115 1104 82-83 0.4372073993182468 1104 84-85 0.4773640178898688 1104 86-87 0.4488035985282508 1104 88-89 0.4134938908260217 1104 90-91 0.48121215770788694 1104 92-93 0.4418161578958184 1104 94-95 0.5495275480970783 1104 96-97 0.5114970196759145 1104 98-99 0.43954924649627003 1104 100 0.5055368423117379 1105 1 0.15117914832601542 1105 2 0.28293999639111433 1105 3 0.271934617254292 1105 4 0.3197481361613299 1105 5 0.2830593716415777 1105 6 0.23474596169369732 1105 7 0.36391052980447114 1105 8 0.3028928447275874 1105 9 0.4664720012624457 1105 10-11 0.37907279238718417 1105 12-13 0.327759806839218 1105 14-15 0.5500371066686114 1105 16-17 0.5189367412791199 1105 18-19 0.4914470264373847 1105 20-21 0.3649118731008727 1105 22-23 0.4647137913046393 1105 24-25 0.43630409886399946 1105 26-27 0.44285185133055194 1105 28-29 0.42495952378923363 1105 30-31 0.47983522907283316 1105 32-33 0.4412773123714189 1105 34-35 0.48613975998766534 1105 36-37 0.4447912703652577 1105 38-39 0.4691493971402707 1105 40-41 0.4223878220245325 1105 42-43 0.4241196973408137 1105 44-45 0.4231992012371748 1105 46-47 0.46827345938931586 1105 48-49 0.29570051358502525 1105 50-51 0.3367835727488142 1105 52-53 0.40887977250123697 1105 54-55 0.3225624493409569 1105 56-57 0.40723824788123153 1105 58-59 0.37340514989832485 1105 60-61 0.37975617637282255 1105 62-63 0.3196848506704484 1105 64-65 0.3225268435610644 1105 66-67 0.24561358560971058 1105 68-69 0.2811932904958354 1105 70-71 0.32887217411875014 1105 72-73 0.31435342981827574 1105 74-75 0.3261738123882907 1105 76-77 0.3481507743533072 1105 78-79 0.37658427304121744 1105 80-81 0.2462643049916906 1105 82-83 0.38823326682580017 1105 84-85 0.3095317287102404 1105 86-87 0.3255663356616054 1105 88-89 0.32930907230423756 1105 90-91 0.2536399379988481 1105 92-93 0.30632374194755485 1105 94-95 0.34235131543171704 1105 96-97 0.27156142433266695 1105 98-99 0.28761210102291557 1105 100 0.22824841467800994 1106 1 0.018295458490200645 1106 2 0.15999529169303273 1106 3 0.12071161752236748 1106 4 0.21892411416117596 1106 5 0.11038211088702354 1106 6 0.20227229592342866 1106 7 0.14216493051307566 1106 8 0.10371719255945067 1106 9 0.25969645228713034 1106 10-11 0.2972546776519991 1106 12-13 0.15404483598390328 1106 14-15 0.3235790065818094 1106 16-17 0.2200717121446516 1106 18-19 0.23540474894275576 1106 20-21 0.21840825815778686 1106 22-23 0.2607633469851933 1106 24-25 0.3314460407392019 1106 26-27 0.2526808459059069 1106 28-29 0.22730901956786198 1106 30-31 0.33014742376533945 1106 32-33 0.27273060739887 1106 34-35 0.3145781693615959 1106 36-37 0.3408506840521852 1106 38-39 0.29613977207162634 1106 40-41 0.3365566745321402 1106 42-43 0.29004461344873533 1106 44-45 0.31602668382561916 1106 46-47 0.3399245004024607 1106 48-49 0.20621220864334333 1106 50-51 0.16302316114798288 1106 52-53 0.2084998846742252 1106 54-55 0.24206400093877534 1106 56-57 0.15525810150996477 1106 58-59 0.18122021702093605 1106 60-61 0.13910992623578977 1106 62-63 0.30439708249498665 1106 64-65 0.22358761424121099 1106 66-67 0.1895707075625097 1106 68-69 0.20126910450650115 1106 70-71 0.19951433474842162 1106 72-73 0.17407112411554948 1106 74-75 0.15290868095544852 1106 76-77 0.17826630661026144 1106 78-79 0.19796885718923818 1106 80-81 0.2674057675748358 1106 82-83 0.21819019570734355 1106 84-85 0.15352334729649897 1106 86-87 0.2488166130018854 1106 88-89 0.28270156176837347 1106 90-91 0.22555939406178283 1106 92-93 0.26207447034114395 1106 94-95 0.2417763949665961 1106 96-97 0.22730761663777344 1106 98-99 0.30207085111203824 1106 100 0.3037103673733803 1107 1 0.10789526305726937 1107 2 0.07434134247547064 1107 3 0.14840096433465177 1107 4 0.16916112333825595 1107 5 0.020377588323036377 1107 6 0.14088983216145579 1107 7 0.15571687307544124 1107 8 0.17707589640843935 1107 9 0.18507671290694105 1107 10-11 0.31028714250007994 1107 12-13 0.18212944505066986 1107 14-15 0.3467112354701669 1107 16-17 0.17341990499645732 1107 18-19 0.11584734215277592 1107 20-21 0.18210490565868298 1107 22-23 0.28546994935117453 1107 24-25 0.2859737037963015 1107 26-27 0.24793700380158867 1107 28-29 0.25905685046789273 1107 30-31 0.336409057651025 1107 32-33 0.2992889160436505 1107 34-35 0.27834262629814077 1107 36-37 0.22666730870164997 1107 38-39 0.27751781775633333 1107 40-41 0.2208370312766803 1107 42-43 0.33822929726627393 1107 44-45 0.2914818220103754 1107 46-47 0.27049783490937784 1107 48-49 0.2665083007820499 1107 50-51 0.2621286677721457 1107 52-53 0.23940766680524916 1107 54-55 0.27700212906778177 1107 56-57 0.3234917914312021 1107 58-59 0.272124669630756 1107 60-61 0.23082240182461788 1107 62-63 0.23684667154903138 1107 64-65 0.22712672754553864 1107 66-67 0.18924323946784938 1107 68-69 0.21708796584385937 1107 70-71 0.2341554547995912 1107 72-73 0.17975531612756157 1107 74-75 0.15808881620953485 1107 76-77 0.10863974152831446 1107 78-79 0.1532633672096253 1107 80-81 0.018811663858080863 1107 82-83 0.04310431572158535 1107 84-85 0.07332600304945203 1107 86-87 0.079676554269966 1107 88-89 0.10620361406165557 1107 90-91 0.09953726114753181 1107 92-93 0.0874524181994012 1107 94-95 0.07620157121843008 1107 96-97 0.18962330565755892 1107 98-99 0.23186976947897975 1107 100 0.2937402875421675 1108 1 0.07759191846769298 1108 2 0.10900408892699787 1108 3 0.025313434609564922 1108 4 0.1932058413576314 1108 5 0.08917959239037287 1108 6 0.0987965000305735 1108 7 0.18171800521309223 1108 8 0.16267704849380493 1108 9 0.11850531158177802 1108 10-11 0.2622227720973953 1108 12-13 0.16571082929514347 1108 14-15 0.21787605445604896 1108 16-17 0.2722968350504331 1108 18-19 0.2019726519017695 1108 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2215 44-45 -0.9674564836267976 2215 46-47 -1.1971354556302032 2215 48-49 -1.2110750674557025 2215 50-51 -1.1721140796901608 2215 52-53 -1.072543908367308 2215 54-55 -1.0268962464620515 2215 56-57 -1.0182655587564184 2215 58-59 -1.1605879307026683 2215 60-61 -1.0667636103973734 2215 62-63 -1.0239965181054345 2215 64-65 -1.0803214984779999 2215 66-67 -0.9790426834012962 2215 68-69 -0.935316890928604 2215 70-71 -1.0787823706903268 2215 72-73 -0.9448573746425453 2215 74-75 -1.046892209134782 2215 76-77 -0.9283490473366207 2215 78-79 -0.9495127776617736 2215 80-81 -0.9064270071929386 2215 82-83 -0.8698031923395781 2215 84-85 -0.9321213831636044 2215 86-87 -0.8445551895107783 2215 88-89 -0.8296440700678822 2215 90-91 -0.9031836697706268 2215 92-93 -0.9494801097113701 2215 94-95 -0.9096633463358579 2215 96-97 -1.133630363883377 2215 98-99 -0.9831239080910699 2215 100 -0.5519398436440675 2216 1 -0.5753837343777235 2216 2 -0.8835602839181149 2216 3 -0.9686757039310976 2216 4 -0.8752459401517214 2216 5 -0.6549768671710012 2216 6 -0.6133926580237485 2216 7 -0.8302467009943442 2216 8 -0.7181360171443743 2216 9 -0.9467169561867976 2216 10-11 -1.3725030398772233 2216 12-13 -0.9372383244325917 2216 14-15 -1.017776295378276 2216 16-17 -1.068887700778511 2216 18-19 -1.3172563256618446 2216 20-21 -1.4336099719688704 2216 22-23 -1.2966216100274153 2216 24-25 -1.3620348421658335 2216 26-27 -1.8428823592769277 2216 28-29 -1.6259544747992436 2216 30-31 -1.7406591629282815 2216 32-33 -1.9483173641118157 2216 34-35 -1.5507901871980891 2216 36-37 -1.6827538512562867 2216 38-39 -1.5442649643080415 2216 40-41 -1.3779822269796966 2216 42-43 -1.3849742960587648 2216 44-45 -1.2322584602762774 2216 46-47 -1.4393010357365696 2216 48-49 -1.4411483117469501 2216 50-51 -1.4761037801341956 2216 52-53 -1.4511567946995 2216 54-55 -1.4624068073587964 2216 56-57 -1.3864787658911126 2216 58-59 -1.26830114408709 2216 60-61 -1.3402073063196696 2216 62-63 -1.418475939783427 2216 64-65 -1.4158454261454025 2216 66-67 -1.374420790824722 2216 68-69 -1.394410236138981 2216 70-71 -1.434147917430785 2216 72-73 -1.3146536767917638 2216 74-75 -1.3415434091213108 2216 76-77 -1.3024653163035964 2216 78-79 -1.1393922190838168 2216 80-81 -1.3648671917870203 2216 82-83 -1.342148262156723 2216 84-85 -1.3791234129230325 2216 86-87 -1.242439949070885 2216 88-89 -1.3853898810265441 2216 90-91 -1.5034908248958274 2216 92-93 -1.3669306073812457 2216 94-95 -1.4211044347175097 2216 96-97 -1.4094631149714054 2216 98-99 -1.3730840093009924 2216 100 -1.041478687505137 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16.0 3 30.0 4 35.0 5 34.0 6 51.0 7 174.0 8 484.0 9 862.0 10 1551.0 11 3342.0 12 6539.0 13 9935.0 14 13222.0 15 16646.0 16 19549.0 17 22816.0 18 25796.0 19 29062.0 20 32482.0 21 36632.0 22 41336.0 23 46994.0 24 53488.0 25 60965.0 26 67329.0 27 73869.0 28 83664.0 29 98708.0 30 113951.0 31 131894.0 32 162294.0 33 207051.0 34 293865.0 35 411755.0 36 657453.0 37 1044265.0 38 832630.0 39 294379.0 40 276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.59418247162792 20.598663876638117 11.613616472995682 24.193537178738282 2 15.888944841160807 19.74229041816776 39.88294285343649 24.48582188723495 3 18.711532490030724 26.603378113355557 28.60187046479702 26.0832189318167 4 12.043529742477556 16.211517498026538 40.57984633965767 31.165106419838235 5 12.889738057947275 38.86424024302777 35.28813584943587 12.957885849589083 6 29.871501445874294 38.709985153130305 18.01870280685097 13.399810594144434 7 27.010236792601027 31.695755652113693 23.834488061296966 17.459519493988314 8 26.51061660121683 34.726328157811736 19.817042778537683 18.946012462433753 9 26.241911280757968 14.767399913142915 21.43284366255818 37.55784514354094 10-11 25.976687098768824 25.70781229157734 27.44626027571592 20.869240333937913 12-13 25.87294544745904 23.64967870917425 27.552672309913227 22.924703533453485 14-15 23.218706394164172 24.913659040690508 24.842398512731396 27.02523605241392 16-17 22.068000360746787 27.43380240288818 26.26697243667806 24.23122479968697 18-19 21.315166433452156 28.549337636736904 27.855046115190945 22.280449814619995 20-21 23.25698449211614 27.556905172447248 26.775188693178325 22.410921642258284 22-23 23.805114389859465 27.20336015871343 26.32225101136504 22.66927444006207 24-25 23.179409408333846 27.0926133756809 27.4194431382194 22.308534077765852 26-27 23.18246732699399 27.29000097849977 26.169199973280215 23.35833172122603 28-29 22.06848616176282 27.976041947020796 26.81460342200993 23.140868469206456 30-31 23.043637512821313 27.608147951582467 26.808951846197754 22.539262689398466 32-33 23.587351303228342 27.698063354206532 25.732582679909534 22.98200266265559 34-35 23.453552676103754 27.391739418157623 26.911519826166554 22.243188079572068 36-37 22.993503087578336 26.938351088632473 26.59111024628615 23.47703557750304 38-39 23.278871142488466 27.167171064747592 27.16371863871863 22.390239154045307 40-41 22.78560774371448 26.169356267881973 27.865260054480807 23.179775933922745 42-43 22.763864134303198 26.45896105105996 27.0856266226682 23.691548191968646 44-45 22.94960601751762 27.460425276132007 27.025850844942838 22.56411786140753 46-47 22.874687277493276 27.235333837979862 26.476392451550357 23.413586432976505 48-49 23.725302424812902 26.868667324430973 26.64646244644598 22.759567804310148 50-51 23.11781970701294 26.505396396150672 26.859915581038496 23.516868315797893 52-53 22.53354630792673 27.287531536262293 26.564405488778224 23.61451666703275 54-55 22.506386520266624 27.123150866530793 26.62364003919919 23.746822574003392 56-57 23.794870359506323 26.503530727962175 26.259397849150417 23.44220106338108 58-59 22.534861066503876 27.014486659916333 27.293479389004798 23.157172884574994 60-61 22.561933787809835 27.75554087964545 26.437702038504344 23.24482329404037 62-63 23.10898656763208 26.892080879609214 25.954371891059907 24.044560661698796 64-65 22.13114243357845 27.182662361600812 26.568721617511127 24.11747358730961 66-67 22.93161957582438 26.66699559108484 26.497425859976463 23.90395897311431 68-69 22.27918211858061 26.750313243112917 26.841750508844502 24.12875412946197 70-71 22.240614031521595 26.377698599364695 27.010150984894153 24.371536384219546 72-73 22.93884960501508 26.34844311446973 27.57642139515101 23.13628588536418 74-75 23.094215912031142 26.69363845014427 27.039939888068492 23.1722057497561 76-77 22.78740026860621 26.61115716813711 26.620493419883317 23.980949143373365 78-79 22.65055463343364 26.612557649077047 26.584047857225084 24.152839860264226 80-81 22.124728608252557 26.971868212014222 27.451223542017907 23.45217963771532 82-83 23.071143366893708 25.74397482711568 28.678547166012763 22.506334639977847 84-85 23.251133244553955 26.460868345670818 27.104517135213335 23.183481274561892 86-87 21.708665247385735 27.214075191926728 27.627959493904658 23.44930006678288 88-89 23.11226897485817 25.76838139909255 27.31137500689475 23.80797461915453 90-91 22.62104306498979 25.84727821586951 27.486811706315905 24.044867012824795 92-93 23.087326925716976 26.072347583497308 27.382386300492257 23.45793919029346 94-95 21.787131785780424 26.139824800975433 28.141025398761588 23.932018014482555 96-97 22.032683982617655 26.00776736674296 27.721997688850376 24.237550961789015 98-99 21.346946200865315 26.78189568427959 27.519059590990885 24.35209852386421 100 22.32797189262919 25.8924305277505 26.536602149832934 25.242995429787385 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 365.0 1 277.5 2 219.5 3 233.5 4 204.5 5 186.0 6 173.0 7 177.0 8 199.0 9 207.0 10 194.5 11 195.5 12 208.0 13 220.5 14 248.0 15 383.5 16 470.0 17 502.5 18 668.5 19 861.5 20 1170.0 21 1590.5 22 2169.0 23 3067.0 24 4259.5 25 5781.0 26 8294.5 27 11351.0 28 15756.5 29 25164.5 30 39022.0 31 52450.0 32 70371.0 33 87585.0 34 99249.5 35 113531.5 36 126453.5 37 147667.0 38 157115.0 39 146795.5 40 135767.0 41 123700.5 42 120806.5 43 130505.0 44 145477.5 45 174935.5 46 204850.0 47 225816.5 48 239522.0 49 235699.5 50 222575.5 51 227288.0 52 231189.5 53 219598.0 54 207681.5 55 177430.5 56 153833.0 57 127169.5 58 98542.0 59 96297.0 60 81544.0 61 55568.0 62 41612.5 63 35137.0 64 25503.0 65 11850.5 66 6107.5 67 4111.5 68 2904.5 69 2063.5 70 1512.5 71 1084.5 72 747.0 73 574.0 74 445.5 75 348.5 76 232.5 77 126.5 78 79.0 79 47.0 80 34.0 81 27.0 82 26.0 83 26.0 84 18.0 85 13.5 86 13.5 87 13.5 88 11.5 89 10.0 90 10.5 91 9.5 92 9.0 93 8.0 94 5.5 95 4.5 96 3.5 97 6.5 98 7.0 99 5.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05642003634414506 2 0.007741923886470304 3 0.007844060085500256 4 0.009641657188427397 5 0.003697330404884235 6 0.002369559817494869 7 8.783713116575807E-4 8 0.0014707612660312978 9 5.311082349557465E-4 10-11 4.698265155377757E-4 12-13 3.0640859708985367E-4 14-15 1.4299067864193172E-4 16-17 4.800401354407708E-4 18-19 0.0015116157456432782 20-21 8.783713116575807E-4 22-23 0.0012256343883594147 24-25 0.0012664888679713954 26-27 0.003574766966048293 28-29 0.004116088820907035 30-31 0.005208946150527513 32-33 0.006352871579662967 34-35 0.003339853708279405 36-37 0.008303672981135036 38-39 0.006444794158789923 40-41 0.00624052176073002 42-43 0.014462485782641095 44-45 0.020028908629773436 46-47 0.018568360983645133 48-49 0.021795864872991594 50-51 0.01853772012393615 52-53 0.025666826816226746 54-55 0.019109682838503877 56-57 0.01539192519381365 58-59 0.01740400831470369 60-61 0.015963887908381377 62-63 0.011612885829705455 64-65 0.011653740309317435 66-67 0.013941591167588344 68-69 0.0054030049286844205 70-71 0.008804140356381796 72-73 0.008722431397157835 74-75 0.011347331712227583 76-77 0.01074472813795087 78-79 0.012552538860781007 80-81 0.010550669359793962 82-83 0.006444794158789923 84-85 0.010591523839405944 86-87 0.009335248591337542 88-89 0.008048332483560157 90-91 0.013226637774378686 92-93 0.008497731759291943 94-95 0.007404874429671464 96-97 0.009345462211240538 98-99 0.009141189813180637 100 0.25981406309238997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4895424.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.83977155690222 #Duplication Level Percentage of deduplicated Percentage of total 1 75.74442746679452 51.384846560556895 2 14.501914278989272 19.67613103648821 3 4.9447724640103194 10.063567030779616 4 2.139804200197043 5.806553124714691 5 1.068877472249305 3.6256201769855965 6 0.5651695960164211 2.300458577079643 7 0.33460889274507283 1.5889853591313614 8 0.20837199556790073 1.130872686254578 9 0.1359748697789996 0.8302053692958176 >10 0.3533369303929307 3.3123764799359363 >50 0.002144235690003459 0.0947865939307399 >100 4.706524975505704E-4 0.06327896998028024 >500 8.463004755715801E-5 0.04185395360960968 >1k 4.231502309854514E-5 0.08046408125706252 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0213619902995123E-5 0.0 18-19 0.0 0.0 0.0 2.0427239805990247E-5 0.0 20-21 0.0 0.0 0.0 3.064085970898537E-5 0.0 22-23 0.0 0.0 0.0 6.128171941797074E-5 0.0 24-25 0.0 0.0 0.0 1.4299067864193172E-4 0.0 26-27 0.0 0.0 0.0 4.800401354407708E-4 0.0 28-29 0.0 0.0 0.0 0.002900668052450615 0.0 30-31 0.0 0.0 0.0 0.011326904472421592 0.0 32-33 0.0 0.0 0.0 0.02821001817207253 0.0 34-35 0.0 0.0 0.0 0.056225977565988156 0.0 36-37 0.0 0.0 0.0 0.10500622622269287 0.0 38-39 0.0 0.0 0.0 0.17943287445581835 0.0 40-41 0.0 0.0 0.0 0.2605290164855996 0.0 42-43 0.0 0.0 0.0 0.34752863081931207 0.0 44-45 0.0 0.0 0.0 0.45169734020995933 0.0 46-47 0.0 0.0 0.0 0.5814715946974154 0.0 48-49 0.0 0.0 0.0 0.7306517270005621 0.0 50-51 0.0 0.0 0.0 0.8927929429606097 0.0 52-53 0.0 0.0 0.0 1.051165333176452 0.0 54-55 0.0 0.0 0.0 1.2241840543331897 0.0 56-57 0.0 0.0 0.0 1.4371584565504438 0.0 58-59 0.0 0.0 0.0 1.69648226588749 0.0 60-61 0.0 0.0 0.0 1.9596157554483535 0.0 62-63 0.0 0.0 0.0 2.236343573100103 0.0 64-65 0.0 0.0 0.0 2.527625799113621 0.0 66-67 0.0 0.0 0.0 2.8577197807585204 0.0 68-69 0.0 0.0 0.0 3.2248892026512923 0.0 70-71 0.0 0.0 0.0 3.630502689858938 0.0 72-73 0.0 0.0 0.0 4.056502562392961 0.0 74-75 0.0 0.0 0.0 4.471737279549227 0.0 76-77 0.0 0.0 0.0 4.947855384947249 0.0 78-79 0.0 0.0 0.0 5.462131574302859 0.0 80-81 0.0 0.0 0.0 6.016996689152972 0.0 82-83 0.0 0.0 0.0 6.597069017923678 0.0 84-85 0.0 0.0 0.0 7.157255428743251 0.0 86-87 0.0 2.0427239805990247E-5 0.0 7.755181165104391 0.0 88 0.0 2.0427239805990247E-5 0.0 8.242922369952021 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 5840 0.0 42.932297 94 GTACTAG 5740 0.0 37.599102 1 GCATCGT 5870 0.0 34.0249 8 CTGTGCG 8095 0.0 33.496582 9 TAGTACT 8300 0.0 32.784504 4 TAGGCAT 7255 0.0 32.32413 5 ACTGTGC 8910 0.0 31.01313 8 TACTGTG 9660 0.0 30.113382 7 CTAACGC 2765 0.0 29.574831 3 GTACTGT 9230 0.0 29.530603 6 TCTAACG 2855 0.0 29.13696 2 CTAGGCA 8295 0.0 28.781637 4 TAACGCC 2920 0.0 28.648727 4 CTAGTAC 10020 0.0 28.282497 3 CGCCTAC 2925 0.0 27.955637 7 CAGTACG 690 0.0 27.923916 9 TATTCTC 2810 0.0 27.093956 5 GTACATG 19895 0.0 27.037018 1 TACATGG 19915 0.0 26.43107 2 CAACGCA 10235 0.0 26.356503 5 >>END_MODULE