FastQCFastQC Report
Mon 16 Jan 2017
ERR1146650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4895424
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94310.192649298610294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77720.1587605077721562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50440.10303499758141481No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC58050.041.96360894
GTATCAA165500.035.7942051
GGTATCA103400.034.766251
GTACTAG60250.034.5183681
CTGTGCG81250.032.6308179
TAGGCAT70900.032.149855
CAACGCA181100.030.9084195
TAGTACT85200.030.8679924
ATCAACG186450.029.9771423
ACTGTGC90250.029.8918368
CAGTACG8600.029.5166829
CTAGGCA82800.028.7512254
GTACTGT93250.028.6766976
TACTGTG101150.028.435457
TATCAAC208800.028.303762
CTAGTAC100350.027.3330023
TATTCTC26950.027.2050365
AACGCAG213000.027.1609026
TCTAACG26200.027.118192
CTAACGC26300.027.012043
Produced by FastQC (version 0.11.5)