##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146650_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4895424
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.081670351740726	33.0	31.0	34.0	30.0	34.0
2	32.143415973774694	33.0	31.0	34.0	30.0	34.0
3	32.30649949830699	34.0	31.0	34.0	30.0	34.0
4	35.925516564040215	37.0	35.0	37.0	35.0	37.0
5	35.83509293577022	37.0	35.0	37.0	35.0	37.0
6	35.87315153906996	37.0	35.0	37.0	35.0	37.0
7	35.788937995973384	37.0	35.0	37.0	33.0	37.0
8	35.7942325322587	37.0	35.0	37.0	33.0	37.0
9	37.494678091213345	39.0	37.0	39.0	34.0	39.0
10-11	37.43018010288792	39.0	37.0	39.0	34.0	39.0
12-13	37.40230692990025	39.0	37.0	39.0	34.0	39.0
14-15	38.615284089794876	40.0	38.0	41.0	34.0	41.0
16-17	38.614235457439435	40.0	38.0	41.0	34.0	41.0
18-19	38.66357081633787	40.0	38.0	41.0	34.0	41.0
20-21	38.661038042874324	40.0	38.0	41.0	34.0	41.0
22-23	38.61915780941548	40.0	38.0	41.0	34.0	41.0
24-25	38.5457271933953	40.0	38.0	41.0	34.0	41.0
26-27	38.40393569586618	40.0	38.0	41.0	34.0	41.0
28-29	38.345396026983565	40.0	38.0	41.0	34.0	41.0
30-31	38.21420289641919	40.0	37.5	41.0	33.5	41.0
32-33	38.07899622177773	40.0	37.0	41.0	33.0	41.0
34-35	37.97924337095213	40.0	37.0	41.0	33.0	41.0
36-37	37.973624450098704	40.0	37.0	41.0	33.0	41.0
38-39	37.8797490268463	40.0	37.0	41.0	33.0	41.0
40-41	37.76568301336105	40.0	37.0	41.0	33.0	41.0
42-43	37.62885727569257	40.0	36.5	41.0	32.5	41.0
44-45	37.417800276339705	40.0	36.0	41.0	32.0	41.0
46-47	37.1761074219516	40.0	36.0	41.0	31.0	41.0
48-49	36.91696715136421	39.0	35.0	41.0	31.0	41.0
50-51	36.70023597547424	39.0	35.0	41.0	30.5	41.0
52-53	36.50699684031454	39.0	35.0	41.0	30.0	41.0
54-55	36.31228040717208	38.5	35.0	41.0	30.0	41.0
56-57	36.07428089987711	38.0	35.0	40.0	30.0	41.0
58-59	35.790226444124144	37.5	35.0	40.0	29.0	41.0
60-61	35.60809268002117	37.0	35.0	40.0	29.5	41.0
62-63	35.38688507880012	36.5	34.5	40.0	29.0	41.0
64-65	35.141238225738974	36.0	34.0	39.5	29.0	41.0
66-67	34.8919829007661	35.5	34.0	39.0	29.0	41.0
68-69	34.63932817668092	35.0	34.0	39.0	29.0	41.0
70-71	34.430545035527054	35.0	34.0	38.0	29.0	41.0
72-73	34.202111911041825	35.0	34.0	37.0	29.0	40.0
74-75	33.95360340595626	35.0	34.0	37.0	29.0	39.5
76-77	33.72055382741107	35.0	33.5	36.0	28.0	39.0
78-79	33.50005505141128	35.0	33.0	36.0	28.0	39.0
80-81	33.37868946591756	35.0	33.0	36.0	28.0	38.0
82-83	33.22398172252291	35.0	33.0	35.0	28.0	37.0
84-85	33.10866821750271	35.0	33.0	35.0	29.0	37.0
86-87	32.97291143729328	35.0	33.0	35.0	28.5	36.5
88-89	32.87187187463231	35.0	33.0	35.0	28.5	36.0
90-91	32.81219359140291	35.0	33.0	35.0	29.0	36.0
92-93	32.744396399576424	35.0	33.0	35.0	28.0	36.0
94-95	32.7043470596214	35.0	33.0	35.0	28.5	35.5
96-97	32.61450601623066	35.0	33.0	35.0	27.0	35.0
98-99	32.54316694529422	35.0	33.0	35.0	27.0	35.0
100	31.271434507000823	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3740011497195752
1101	2	0.42105661843564235
1101	3	0.35653500269618377
1101	4	0.27826101490091304
1101	5	0.21166837085331736
1101	6	0.2719918657793059
1101	7	0.265156186436208
1101	8	0.2791883365787484
1101	9	0.343239197599182
1101	10-11	0.3738265123025286
1101	12-13	0.3677309236177777
1101	14-15	0.5038172726662964
1101	16-17	0.5529141098235684
1101	18-19	0.5225309451352942
1101	20-21	0.5108149752741866
1101	22-23	0.5234534213993527
1101	24-25	0.4982535673977182
1101	26-27	0.5668747063237447
1101	28-29	0.538353713815539
1101	30-31	0.5408890927725309
1101	32-33	0.6174798689879566
1101	34-35	0.618670059426087
1101	36-37	0.5213235421068987
1101	38-39	0.5093127084734519
1101	40-41	0.5071242283421284
1101	42-43	0.5663420017894651
1101	44-45	0.5466838958102045
1101	46-47	0.5664920987803796
1101	48-49	0.4370148219969323
1101	50-51	0.49848851253625526
1101	52-53	0.541166591497884
1101	54-55	0.5672048679037616
1101	56-57	0.5289067510920944
1101	58-59	0.5191611955368458
1101	60-61	0.5073573681717463
1101	62-63	0.49336706141062336
1101	64-65	0.48433088093377563
1101	66-67	0.503061643154247
1101	68-69	0.54564205417865
1101	70-71	0.5080387021744528
1101	72-73	0.5524814659856787
1101	74-75	0.5339077792378291
1101	76-77	0.42918722565590883
1101	78-79	0.44391594945848567
1101	80-81	0.4879071760463063
1101	82-83	0.44390723033868085
1101	84-85	0.46581003802042886
1101	86-87	0.39604209435994875
1101	88-89	0.46455059366571305
1101	90-91	0.48167430575646364
1101	92-93	0.4688180786104681
1101	94-95	0.45074802694814053
1101	96-97	0.4205604055834158
1101	98-99	0.48803914326529707
1101	100	0.7935679541409222
1102	1	0.40667703413964773
1102	2	0.43243120539162305
1102	3	0.3399543087899062
1102	4	0.23743182000195162
1102	5	0.25665800014527207
1102	6	0.2726412821264006
1102	7	0.3349770614896457
1102	8	0.3202285462236105
1102	9	0.3608785466338773
1102	10-11	0.34136298565294965
1102	12-13	0.40947908309849623
1102	14-15	0.5473042173160323
1102	16-17	0.5385538193004891
1102	18-19	0.4853939007308057
1102	20-21	0.46910272571310685
1102	22-23	0.511269374331583
1102	24-25	0.506472366314199
1102	26-27	0.5685512327095807
1102	28-29	0.6177280184805483
1102	30-31	0.6747495696562638
1102	32-33	0.6386537389990394
1102	34-35	0.6267263523660986
1102	36-37	0.6338473712004955
1102	38-39	0.6660470846845712
1102	40-41	0.6165875921659492
1102	42-43	0.6083738432399173
1102	44-45	0.6101989346159513
1102	46-47	0.6634141709931427
1102	48-49	0.6714596392905818
1102	50-51	0.6644005752506459
1102	52-53	0.6511624805635421
1102	54-55	0.6040603727825271
1102	56-57	0.6327885366599162
1102	58-59	0.5909414315612693
1102	60-61	0.5830111269650402
1102	62-63	0.5895557142853249
1102	64-65	0.5061922356439723
1102	66-67	0.5451386358126413
1102	68-69	0.5312940747143884
1102	70-71	0.49162729332118005
1102	72-73	0.5799924934967109
1102	74-75	0.552643388089713
1102	76-77	0.4775547856513711
1102	78-79	0.5722434847627653
1102	80-81	0.49676766891843016
1102	82-83	0.5070198094396332
1102	84-85	0.47651539901648476
1102	86-87	0.5055827300633737
1102	88-89	0.5157676361618257
1102	90-91	0.5262510349262115
1102	92-93	0.48914715417210175
1102	94-95	0.48039270484863295
1102	96-97	0.45669839957025715
1102	98-99	0.5026931724542152
1102	100	0.7718041126096402
1103	1	0.2621514744672311
1103	2	0.31899952492687333
1103	3	0.2442752457166364
1103	4	0.19592452769156665
1103	5	0.20273842807261389
1103	6	0.24287901326502492
1103	7	0.2577232350302907
1103	8	0.18635679994397947
1103	9	0.2497954575994612
1103	10-11	0.3302330484522997
1103	12-13	0.3018454093539802
1103	14-15	0.3759854845765105
1103	16-17	0.3286816530735237
1103	18-19	0.35206200223856143
1103	20-21	0.3214611896282733
1103	22-23	0.3262889380664902
1103	24-25	0.3442186535800644
1103	26-27	0.38644395925770425
1103	28-29	0.4428077373366577
1103	30-31	0.388828780392501
1103	32-33	0.3958379394396019
1103	34-35	0.4232518291973477
1103	36-37	0.3829908120802443
1103	38-39	0.42767973152662364
1103	40-41	0.4207311524492994
1103	42-43	0.3536692469459979
1103	44-45	0.4053634076266803
1103	46-47	0.45720518719281955
1103	48-49	0.5028334103050227
1103	50-51	0.4857110995844849
1103	52-53	0.4654722080637157
1103	54-55	0.36275963449605797
1103	56-57	0.3586955804720162
1103	58-59	0.40281048332605707
1103	60-61	0.42196650576261163
1103	62-63	0.40439493442621455
1103	64-65	0.4041478016070741
1103	66-67	0.3612584555696756
1103	68-69	0.32879080594238275
1103	70-71	0.35101843971967384
1103	72-73	0.3972969641155828
1103	74-75	0.348315184421061
1103	76-77	0.34421989812593523
1103	78-79	0.3900956188204745
1103	80-81	0.3198208311530877
1103	82-83	0.3006684246276521
1103	84-85	0.2883899241032779
1103	86-87	0.34307311192238643
1103	88-89	0.3175686067411263
1103	90-91	0.32499019447568855
1103	92-93	0.3188255203195496
1103	94-95	0.3202963958330116
1103	96-97	0.36241687907950393
1103	98-99	0.3719294370895554
1103	100	0.6142061148296918
1104	1	0.27311209673842995
1104	2	0.297162854591555
1104	3	0.19784043690379605
1104	4	0.18016927585578912
1104	5	0.18789081989769585
1104	6	0.1928818520590525
1104	7	0.21528375955767132
1104	8	0.2226093081808429
1104	9	0.20585620895520407
1104	10-11	0.3072484707531373
1104	12-13	0.3235439921314551
1104	14-15	0.37370904062837695
1104	16-17	0.34250265710222294
1104	18-19	0.3683141806061485
1104	20-21	0.4020117461757735
1104	22-23	0.41628406251046357
1104	24-25	0.4566244334873488
1104	26-27	0.5059853635992937
1104	28-29	0.5131492248405181
1104	30-31	0.4699146984316869
1104	32-33	0.45074425679953833
1104	34-35	0.524135517241568
1104	36-37	0.4577008584756257
1104	38-39	0.43094079911664096
1104	40-41	0.5242700759474701
1104	42-43	0.5344300267651505
1104	44-45	0.43057650546227677
1104	46-47	0.45439043368387644
1104	48-49	0.48390922786629886
1104	50-51	0.4736250696130142
1104	52-53	0.43857489012896167
1104	54-55	0.45419785056927253
1104	56-57	0.44094352516077606
1104	58-59	0.42695480783466166
1104	60-61	0.4300008274506695
1104	62-63	0.4208917632236293
1104	64-65	0.3772953729381996
1104	66-67	0.3644886509798013
1104	68-69	0.4404025961497311
1104	70-71	0.4205006004502607
1104	72-73	0.36805056967094885
1104	74-75	0.39999621195789814
1104	76-77	0.35150082699129115
1104	78-79	0.3402165297483677
1104	80-81	0.31053260525848003
1104	82-83	0.26251531587931964
1104	84-85	0.26993483056229906
1104	86-87	0.27098041632731196
1104	88-89	0.2776438263711043
1104	90-91	0.2942781065385134
1104	92-93	0.29030420937247925
1104	94-95	0.2332390104803892
1104	96-97	0.22140961646798019
1104	98-99	0.28047692877721886
1104	100	0.49578019695308484
1105	1	0.20228627856650405
1105	2	0.182002446797199
1105	3	0.156632469928482
1105	4	0.15828774745573782
1105	5	0.16798922183708953
1105	6	0.13145564518690378
1105	7	0.18435769131187385
1105	8	0.17727935405179096
1105	9	0.2372754708489353
1105	10-11	0.2028900625739425
1105	12-13	0.2451248522757581
1105	14-15	0.2604279436028136
1105	16-17	0.3007132368361809
1105	18-19	0.27066378175127426
1105	20-21	0.32276009199458855
1105	22-23	0.30804476708620143
1105	24-25	0.26276635648916624
1105	26-27	0.2744131947611095
1105	28-29	0.31593389869910027
1105	30-31	0.374800890167478
1105	32-33	0.36948282955833633
1105	34-35	0.2660310312252605
1105	36-37	0.3511019287673136
1105	38-39	0.3461956204968146
1105	40-41	0.3981110399749994
1105	42-43	0.38911849380752983
1105	44-45	0.4431366560270078
1105	46-47	0.39709510986092056
1105	48-49	0.43871576962484227
1105	50-51	0.41874342015071875
1105	52-53	0.4451198297207952
1105	54-55	0.3798140807938779
1105	56-57	0.3151171826676915
1105	58-59	0.3643671011360041
1105	60-61	0.3368532966845237
1105	62-63	0.3708142225656488
1105	64-65	0.358373974077999
1105	66-67	0.32342414048303425
1105	68-69	0.30142158243008765
1105	70-71	0.3635782943713437
1105	72-73	0.33726401411092866
1105	74-75	0.4102961057665979
1105	76-77	0.36915387770121555
1105	78-79	0.33089076901757863
1105	80-81	0.25320643853658
1105	82-83	0.33276991835867165
1105	84-85	0.3501967868734681
1105	86-87	0.3293535339916147
1105	88-89	0.3125420989605914
1105	90-91	0.32217127616915775
1105	92-93	0.3117687284431341
1105	94-95	0.3741699166621686
1105	96-97	0.3712412319664935
1105	98-99	0.3453069703364946
1105	100	0.5585437317571476
1106	1	0.11845147319792204
1106	2	0.17973855531372607
1106	3	0.13770937822257423
1106	4	0.12710175581695893
1106	5	0.10070248252315395
1106	6	0.12372391431520668
1106	7	0.14205419402598807
1106	8	0.0427471793845271
1106	9	0.061321365331082234
1106	10-11	0.16447305075992347
1106	12-13	0.11210287562983723
1106	14-15	0.13526777495743403
1106	16-17	0.1538917762565788
1106	18-19	0.20287499008091459
1106	20-21	0.15544820933386205
1106	22-23	0.17190942055587755
1106	24-25	0.10113672325810796
1106	26-27	0.19198190947385285
1106	28-29	0.10969157970244225
1106	30-31	0.1440333303300747
1106	32-33	0.18073483022890713
1106	34-35	0.21672620914598895
1106	36-37	0.13591835646091255
1106	38-39	0.13456763878529898
1106	40-41	0.0837462124728674
1106	42-43	0.15155358940910446
1106	44-45	0.14208666973750184
1106	46-47	0.07557268612278989
1106	48-49	0.14690376710916553
1106	50-51	0.1960328638678277
1106	52-53	0.12416358649137749
1106	54-55	0.1429251653841348
1106	56-57	0.10188320202388468
1106	58-59	0.03760838616003781
1106	60-61	0.06250480357311972
1106	62-63	0.13863825517130834
1106	64-65	0.06530488408378687
1106	66-67	0.11111287051370056
1106	68-69	0.10420900241284414
1106	70-71	0.12940146473686553
1106	72-73	0.11834818334101271
1106	74-75	0.11053810608706272
1106	76-77	0.11073147944184569
1106	78-79	0.12876273018968476
1106	80-81	0.06037742901130372
1106	82-83	0.09129500152242542
1106	84-85	0.1028393459122583
1106	86-87	0.08966356797267139
1106	88-89	0.09701014228113536
1106	90-91	0.07111283422738524
1106	92-93	0.16129135624136381
1106	94-95	0.16049871458849907
1106	96-97	0.0580975705943132
1106	98-99	0.09736860657901047
1106	100	0.2770394792061417
1107	1	0.09776287395449401
1107	2	0.13738159526251081
1107	3	0.1016484303257954
1107	4	0.10239570338106319
1107	5	0.017256456802250852
1107	6	0.0538920496363815
1107	7	0.08593812052562555
1107	8	0.093097831615367
1107	9	0.06315147157788203
1107	10-11	0.12510263883814332
1107	12-13	-0.018741861598748244
1107	14-15	0.09519699651030322
1107	16-17	0.06848052188222908
1107	18-19	0.10978507709115348
1107	20-21	0.10988204056881301
1107	22-23	0.12494198408585788
1107	24-25	0.11846029262336799
1107	26-27	0.11853397182933634
1107	28-29	0.13605522073215326
1107	30-31	0.11320573106181797
1107	32-33	0.20913034838419264
1107	34-35	0.1380002334149495
1107	36-37	0.047271542555755275
1107	38-39	0.09129578600696675
1107	40-41	0.15620713253095886
1107	42-43	0.10750954312491956
1107	44-45	0.09879682966236203
1107	46-47	0.1358700690696324
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2216	82-83	-1.1531700564588547
2216	84-85	-1.13738751000394
2216	86-87	-1.0891668551029952
2216	88-89	-1.0425990353755665
2216	90-91	-1.0732588669609058
2216	92-93	-1.1720550931241647
2216	94-95	-1.1635622909589785
2216	96-97	-1.1723511621283258
2216	98-99	-1.1766252991522492
2216	100	-0.5667256916767052
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	14.0
13	69.0
14	245.0
15	795.0
16	1823.0
17	3736.0
18	6678.0
19	10608.0
20	15071.0
21	21671.0
22	29658.0
23	34705.0
24	36398.0
25	36461.0
26	39631.0
27	48595.0
28	60906.0
29	77598.0
30	97060.0
31	120558.0
32	151595.0
33	195189.0
34	276341.0
35	400477.0
36	649195.0
37	1193489.0
38	1003758.0
39	382457.0
40	641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.842514151991736	21.87647076126603	12.891059078845876	25.38995600789635
2	14.566280967491322	20.644980595874276	39.323420413054464	25.46531802357994
3	17.347302297002262	26.908721287471728	29.374146141376112	26.3698302741499
4	11.629758449049323	17.044792402265156	39.18763757012166	32.13781157856386
5	12.382579044649379	38.9119318321101	35.436144899256576	13.269344223983945
6	29.880196256789247	39.15972123348687	17.408546718026205	13.551535791697674
7	26.168994997980764	32.316921336521894	23.93143554704057	17.58264811845677
8	26.742218855813103	34.500995215123346	19.4292874325084	19.32749849655515
9	26.22749326718176	14.110687858702331	21.523712757056384	38.13810611705952
10-11	25.824943865945016	25.842450010458744	27.294244584330187	21.038361539266056
12-13	25.97068704030294	23.544139241410043	28.01241529951057	22.47275841877644
14-15	23.14147519975093	24.615753596905883	25.659917086332072	26.582854117011113
16-17	21.62905603273588	26.99342283732727	27.10286177458786	24.274659355348994
18-19	20.951810156412538	27.35243629274263	29.647020035770172	22.04873351507465
20-21	22.592997051940753	26.588432380933703	28.819863202860468	21.998707364265076
22-23	23.002992182086782	26.177109480200283	28.51333204233178	22.306566295381156
24-25	22.47502974206116	26.151401798904445	29.479867320992014	21.893701138042385
26-27	22.52718048528585	26.303145549803247	28.265580264344827	22.90409370056608
28-29	21.568142003634414	27.00993826071041	28.826645046476056	22.595274689179117
30-31	22.639969489874627	26.579362686459845	28.74387387078218	22.036793952883347
32-33	23.178002559124604	26.74900069942869	27.822830055169888	22.250166686276817
34-35	22.88281873030814	26.59173138016237	28.751084686433696	21.774365203095787
36-37	22.592935770221334	25.986033079055055	28.436280493783585	22.98475065694003
38-39	22.78928239923651	26.14769425487966	29.158894101920485	21.90412924396334
40-41	22.181030693153442	25.387811147716725	29.666398661280414	22.764759497849422
42-43	22.50536419317305	25.535581800473256	28.686922726203086	23.272131280150607
44-45	22.684991126407027	26.525414346132226	28.4496501222366	22.339944405224145
46-47	22.722352548012186	26.356419791217267	27.69666120850819	23.22456645226236
48-49	23.69305498359284	26.246112696264923	27.5902965708384	22.470535749303842
50-51	22.883165993384843	26.05572060765319	27.55135203814828	23.50976136081369
52-53	22.190988972558863	26.79701492659267	27.28960760089422	23.722388499954242
54-55	22.299835519865084	26.755384211868062	27.116997424533608	23.82778284373325
56-57	23.590796221124055	26.027296103463154	26.88011293812344	23.501794737289355
58-59	22.224174159467168	26.568498749290537	27.938922113243976	23.26840497799832
60-61	22.371575986063718	27.31986034304689	26.994086722620963	23.31447694826842
62-63	22.94633723248487	26.405067262815233	26.468585764991957	24.180009739707938
64-65	21.986247545408286	26.72197281661603	27.037839677396224	24.25393996057946
66-67	22.693354629448717	26.225986333540025	26.99884207990108	24.081816957110178
68-69	22.20342916160071	26.125244718332873	27.459357963682002	24.211968156384412
70-71	22.17283909762579	25.855196217998255	27.4228116739051	24.549153010470857
72-73	22.82162935750731	25.73389274261848	27.936806824036104	23.5076710758381
74-75	22.90663364790425	26.262029536512703	27.323301667882603	23.508035147700436
76-77	22.620182791336717	26.280554592069567	26.812368218607684	24.286894397986035
78-79	22.37850996298174	26.402739130559123	26.90351311899158	24.315237787467556
80-81	21.948129518505446	26.87943883920984	27.603431286033654	23.569000356251063
82-83	22.88843622125479	25.642753314115385	28.718268325685376	22.750542138944454
84-85	23.218080803623955	26.343009308284635	27.02378792929887	23.415121958792536
86-87	21.58115415539083	27.062313703572972	27.569307581937746	23.787224559098455
88-89	22.69699212979305	25.827446202821246	27.315274427710452	24.160287239675256
90-91	22.221660473127557	25.7836399870573	27.50361357872168	24.49108596109346
92-93	22.780130995803425	26.14232189081068	27.092096619210103	23.985450494175787
94-95	21.478313216587573	26.167733377129338	27.812820707664955	24.54113269861814
96-97	21.79380172177119	26.05797781765175	27.37244005830751	24.775780402269547
98-99	21.072270757344	26.897476091958534	27.224352783333988	24.80590036736348
100	22.017432390364867	26.066928065993146	26.1721936551626	25.74344588847939
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	237.0
1	159.5
2	142.5
3	174.0
4	103.0
5	81.0
6	199.0
7	447.5
8	711.5
9	766.5
10	571.0
11	431.0
12	454.5
13	542.5
14	706.0
15	1031.0
16	1390.5
17	1851.5
18	2554.0
19	3384.5
20	4442.5
21	5614.5
22	6868.5
23	8256.0
24	9705.0
25	11386.0
26	13976.5
27	16577.5
28	20380.5
29	29513.0
30	42517.0
31	54068.0
32	70967.5
33	87929.5
34	98245.5
35	111549.5
36	123733.0
37	144180.5
38	153942.5
39	143428.0
40	131891.0
41	120861.5
42	118764.5
43	126670.0
44	141389.0
45	173099.0
46	202731.5
47	223516.5
48	239879.5
49	234461.5
50	219689.0
51	224603.0
52	228009.5
53	215991.0
54	208164.5
55	179199.5
56	152801.0
57	126696.5
58	97991.5
59	97175.0
60	81674.0
61	54109.0
62	39603.0
63	32942.5
64	23716.5
65	10353.0
66	4984.0
67	3342.0
68	2417.5
69	1722.0
70	1188.5
71	825.5
72	576.0
73	472.0
74	337.0
75	224.0
76	142.5
77	62.5
78	28.0
79	13.5
80	4.0
81	0.5
82	0.5
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006761416375782771
3	0.0
4	0.08746944084925024
5	0.004412283798093893
6	2.0427239805990247E-5
7	2.0427239805990247E-5
8	0.0
9	0.0
10-11	0.0
12-13	2.5534049757487806E-4
14-15	0.009253539632113582
16-17	0.0
18-19	9.192257912695611E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	5.106809951497562E-5
60-61	0.0
62-63	0.0
64-65	1.4299067864193172E-4
66-67	1.736315383509171E-4
68-69	0.0
70-71	5.515354747617367E-4
72-73	4.4939927573178545E-4
74-75	5.208946150527513E-4
76-77	1.225634388359415E-4
78-79	4.0854479611980494E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.062037527290792385
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4895424.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.07373436367318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72046123422994	46.59471515925843
2	16.13245871924624	20.67333749219814
3	5.649897663916245	10.860301262991214
4	2.4278575081701574	6.222475882053766
5	1.2229718986494462	3.9180188284150828
6	0.6511473125180162	2.5032863960339435
7	0.3839140801199204	1.7219166151654406
8	0.23166127785808638	1.187472254386249
9	0.15921994949644494	0.9181635074489405
>10	0.4124481895188293	3.696153425875042
>50	0.0038749529180325494	0.16870469862978285
>100	0.003223639563968104	0.4392905587089802
>500	4.999637786641044E-4	0.23743423215992135
>1k	2.2725625996177645E-4	0.21084555673778899
>5k	1.363537559770658E-4	0.6478841299372362
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9431	0.192649298610294	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7772	0.1587605077721562	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5044	0.10303499758141481	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	1.0213619902995123E-5	0.0
18-19	0.0	0.0	0.0	2.0427239805990247E-5	0.0
20-21	0.0	0.0	0.0	3.064085970898537E-5	0.0
22-23	0.0	0.0	0.0	6.128171941797074E-5	0.0
24-25	0.0	0.0	0.0	1.6341791844792198E-4	0.0
26-27	0.0	0.0	0.0	5.004673752467611E-4	0.0
28-29	0.0	0.0	0.0	0.00291088167235361	0.0
30-31	0.0	0.0	0.0	0.011398399811742559	0.0
32-33	0.0	0.0	0.0	0.028598135728386348	0.0
34-35	0.0	0.0	0.0	0.056828581140264865	0.0
36-37	0.0	0.0	0.0	0.10631356957027624	0.0
38-39	0.0	0.0	0.0	0.18187392961263416	0.0
40-41	0.0	0.0	0.0	0.2642569877501928	0.0
42-43	0.0	0.0	0.0	0.35243116837274974	0.0
44-45	0.0	0.0	0.0	0.45769273509301744	0.0
46-47	0.0	0.0	0.0	0.588570060529997	0.0
48-49	0.0	0.0	0.0	0.739220954099175	0.0
50-51	0.0	0.0	0.0	0.9028227177053509	0.0
52-53	0.0	0.0	0.0	1.0630335595037326	0.0
54-55	0.0	0.0	0.0	1.237768168804173	0.0
56-57	0.0	0.0	0.0	1.453438966675818	0.0
58-59	0.0	0.0	0.0	1.7167767286347413	0.0
60-61	0.0	0.0	0.0	1.983454344301944	0.0
62-63	0.0	0.0	0.0	2.2635628701415853	0.0
64-65	0.0	0.0	0.0	2.5579602502255168	0.0
66-67	0.0	0.0	0.0	2.892374593089383	0.0
68-69	0.0	0.0	0.0	3.264793815612294	0.0
70-71	0.0	0.0	0.0	3.6755447536311463	0.0
72-73	0.0	0.0	0.0	4.107059980912787	0.0
74-75	0.0	0.0	0.0	4.527187022002588	0.0
76-77	0.0	0.0	0.0	5.010791710789505	0.0
78-79	0.0	0.0	0.0	5.533483922945184	0.0
80-81	0.0	0.0	0.0	6.09658121543711	0.0
82-83	0.0	0.0	0.0	6.685764093161287	0.0
84-85	0.0	0.0	0.0	7.254519731079474	0.0
86-87	0.0	0.0	0.0	7.861035121779032	0.0
88	0.0	0.0	0.0	8.357171922186923	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5805	0.0	41.963608	94
GTATCAA	16550	0.0	35.794205	1
GGTATCA	10340	0.0	34.76625	1
GTACTAG	6025	0.0	34.518368	1
CTGTGCG	8125	0.0	32.630817	9
TAGGCAT	7090	0.0	32.14985	5
CAACGCA	18110	0.0	30.908419	5
TAGTACT	8520	0.0	30.867992	4
ATCAACG	18645	0.0	29.977142	3
ACTGTGC	9025	0.0	29.891836	8
CAGTACG	860	0.0	29.516682	9
CTAGGCA	8280	0.0	28.751225	4
GTACTGT	9325	0.0	28.676697	6
TACTGTG	10115	0.0	28.43545	7
TATCAAC	20880	0.0	28.30376	2
CTAGTAC	10035	0.0	27.333002	3
TATTCTC	2695	0.0	27.205036	5
AACGCAG	21300	0.0	27.160902	6
TCTAACG	2620	0.0	27.11819	2
CTAACGC	2630	0.0	27.01204	3
>>END_MODULE