FastQCFastQC Report
Mon 16 Jan 2017
ERR1146649_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146649_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4451652
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC46100.042.8262294
GTACTAG49500.034.861321
CTGTGCG62250.032.8378149
TAGGCAT61350.032.2479175
TAGTACT65800.031.997824
GCATCGT50750.031.8527378
ACTGTGC70650.030.7294088
CAACGCA73400.030.2830225
CTAACGC25650.029.3157423
CGCCTAC25900.029.2112737
TCTAACG25950.029.1582642
GTATCAA87550.029.0552161
TAACGCC26300.028.9485974
CTAGGCA70000.028.93664
GTACTGT72550.028.888296
GGTATCA59500.028.7652021
TACTGTG79950.028.4479687
CTAGTAC78200.028.3062573
ATCAACG79500.027.8433863
ACGCCTA29350.026.09786
Produced by FastQC (version 0.11.5)