##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146649_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4451652
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.087216386186522	31.0	31.0	34.0	28.0	34.0
2	31.24268024544596	31.0	31.0	34.0	28.0	34.0
3	31.35508772922951	31.0	31.0	34.0	28.0	34.0
4	34.823224501825386	37.0	35.0	37.0	32.0	37.0
5	34.96446981929405	37.0	35.0	37.0	32.0	37.0
6	35.066227548784134	37.0	35.0	37.0	32.0	37.0
7	35.00770028744385	37.0	35.0	37.0	32.0	37.0
8	34.98202532453121	37.0	35.0	37.0	32.0	37.0
9	36.47513147928005	39.0	37.0	39.0	32.0	39.0
10-11	36.31261428341658	39.0	37.0	39.0	31.0	39.0
12-13	36.23050600091831	39.0	37.0	39.0	30.5	39.0
14-15	37.36528237157801	40.0	37.0	41.0	31.0	41.0
16-17	37.37595672348153	40.0	37.0	41.0	31.0	41.0
18-19	37.3284157207257	40.0	37.0	41.0	31.0	41.0
20-21	37.38370721700618	40.0	37.0	41.0	31.0	41.0
22-23	37.3114862752075	40.0	37.0	41.0	30.5	41.0
24-25	37.25033830137666	40.0	36.5	41.0	30.0	41.0
26-27	37.27525085069543	40.0	36.0	41.0	30.0	41.0
28-29	37.246102008872214	40.0	36.0	41.0	30.0	41.0
30-31	37.10058659122501	40.0	36.0	41.0	30.0	41.0
32-33	37.08605546884617	40.0	36.0	41.0	30.0	41.0
34-35	37.022392810579085	40.0	36.0	41.0	30.0	41.0
36-37	36.90422746432111	40.0	36.0	41.0	30.0	41.0
38-39	36.80437969994061	40.0	35.0	41.0	30.0	41.0
40-41	36.71528524691508	39.0	35.0	41.0	29.5	41.0
42-43	36.559612476446944	39.0	35.0	41.0	29.0	41.0
44-45	36.41470357521207	39.0	35.0	41.0	29.0	41.0
46-47	36.42203141665161	39.0	35.0	41.0	29.0	41.0
48-49	36.283630885792505	39.0	35.0	41.0	28.0	41.0
50-51	36.108739070349614	39.0	35.0	41.0	28.0	41.0
52-53	35.89788173019814	38.0	34.0	40.0	28.0	41.0
54-55	35.68028194926288	38.0	34.0	40.0	28.0	41.0
56-57	35.43921548674514	37.5	34.0	40.0	27.5	41.0
58-59	35.13870019489394	37.0	34.0	40.0	27.0	41.0
60-61	34.88712516162539	36.5	33.0	40.0	26.5	41.0
62-63	34.58147323735099	36.0	33.0	39.5	26.0	41.0
64-65	34.42381176695753	35.5	33.0	39.0	26.0	41.0
66-67	34.159026244639065	35.0	33.0	39.0	26.0	41.0
68-69	34.033065028443374	35.0	33.0	39.0	26.0	41.0
70-71	33.94700731324012	35.0	33.0	38.5	26.0	40.5
72-73	33.715248294341066	35.0	33.0	37.5	26.0	40.0
74-75	33.45847013647967	35.0	33.0	37.0	26.0	39.5
76-77	33.18994016154003	35.0	33.0	36.5	26.0	39.0
78-79	32.9371489505469	35.0	32.0	36.0	25.0	39.0
80-81	32.74506217017862	35.0	32.0	36.0	25.0	38.0
82-83	32.48906507067488	35.0	32.0	35.0	25.0	37.0
84-85	32.312298557928614	35.0	32.0	35.0	25.0	37.0
86-87	32.137842648077616	35.0	32.0	35.0	25.0	36.5
88-89	32.009945633665886	35.0	32.0	35.0	25.0	36.0
90-91	31.86466642046593	35.0	32.0	35.0	24.0	36.0
92-93	31.71586267300319	35.0	31.5	35.0	24.0	36.0
94-95	31.641746142780253	35.0	31.0	35.0	24.0	35.0
96-97	31.537121500063346	35.0	31.0	35.0	23.5	35.0
98-99	31.44373504487772	35.0	31.0	35.0	23.0	35.0
100	30.446123596363776	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3508098763960241
1101	2	0.4917209262807276
1101	3	0.511118998324033
1101	4	0.5507902817559582
1101	5	0.49782146212922385
1101	6	0.47898258890977985
1101	7	0.56169353468173
1101	8	0.5674869578842774
1101	9	0.6808073244450839
1101	10-11	0.7423079882928221
1101	12-13	0.6727581776741118
1101	14-15	0.8888537745372673
1101	16-17	0.8953862502000476
1101	18-19	0.9255634565715312
1101	20-21	0.8720164411593956
1101	22-23	0.8567285834850935
1101	24-25	0.9129788955400215
1101	26-27	0.8186816455433004
1101	28-29	0.8343720248848214
1101	30-31	0.8599480888424296
1101	32-33	0.7838591249363844
1101	34-35	0.8207499068630995
1101	36-37	0.859456727339456
1101	38-39	0.7925898846272403
1101	40-41	0.809533023206157
1101	42-43	0.7603558740591083
1101	44-45	0.7595275654196811
1101	46-47	0.7520213504864373
1101	48-49	0.744694286455541
1101	50-51	0.6880771920051032
1101	52-53	0.7303581287249088
1101	54-55	0.6693939496132799
1101	56-57	0.6534097508470609
1101	58-59	0.6523628795346355
1101	60-61	0.6521543968888608
1101	62-63	0.619232050606854
1101	64-65	0.6692084632832405
1101	66-67	0.692242418582623
1101	68-69	0.6630194775784588
1101	70-71	0.7082912151051701
1101	72-73	0.7177861170146826
1101	74-75	0.6527294622421209
1101	76-77	0.6436980929562353
1101	78-79	0.7240205373148072
1101	80-81	0.6722141747731598
1101	82-83	0.6647636082142796
1101	84-85	0.6781388384705949
1101	86-87	0.6143884227856304
1101	88-89	0.7126421005155734
1101	90-91	0.6539912998419197
1101	92-93	0.6735470044588006
1101	94-95	0.7040315072639984
1101	96-97	0.6370194312417858
1101	98-99	0.6824024365325769
1101	100	0.7129030939479719
1102	1	0.3622253725211273
1102	2	0.4391619885931348
1102	3	0.45574987894963215
1102	4	0.5376565413896799
1102	5	0.3815310216153307
1102	6	0.41420377720726265
1102	7	0.37707493732752795
1102	8	0.45530283325914667
1102	9	0.5545180900548417
1102	10-11	0.6600637394119246
1102	12-13	0.5709466499728109
1102	14-15	0.7465295882214562
1102	16-17	0.694156595725552
1102	18-19	0.7352541847956573
1102	20-21	0.7091040033211371
1102	22-23	0.7455405024951887
1102	24-25	0.7612801945792853
1102	26-27	0.7415965501404216
1102	28-29	0.777389717896682
1102	30-31	0.7388341730451131
1102	32-33	0.7072201822727351
1102	34-35	0.7838477194582509
1102	36-37	0.77562561593939
1102	38-39	0.7410571492059432
1102	40-41	0.6930618414179364
1102	42-43	0.7500809658385137
1102	44-45	0.703907194290359
1102	46-47	0.6956024847270683
1102	48-49	0.7060122744746451
1102	50-51	0.6861706502124534
1102	52-53	0.7026011196297048
1102	54-55	0.6349584102746917
1102	56-57	0.7030358115206781
1102	58-59	0.6789370653463251
1102	60-61	0.6379864326365663
1102	62-63	0.6838155513804196
1102	64-65	0.6884460025667423
1102	66-67	0.7308452558472425
1102	68-69	0.6341386772299913
1102	70-71	0.5780128646790246
1102	72-73	0.6234602895020629
1102	74-75	0.5565933523735893
1102	76-77	0.6491515645370853
1102	78-79	0.5683052651059128
1102	80-81	0.6071490555079606
1102	82-83	0.6256304687603915
1102	84-85	0.6161159295666963
1102	86-87	0.585268048565105
1102	88-89	0.6039422173852529
1102	90-91	0.5800188477238493
1102	92-93	0.6075853029952647
1102	94-95	0.6313840849062373
1102	96-97	0.6076387864839354
1102	98-99	0.5040573441030212
1102	100	0.6307572096250027
1103	1	0.26719644590851743
1103	2	0.3788530764707616
1103	3	0.38012068472859184
1103	4	0.47183330976108095
1103	5	0.2681218024271885
1103	6	0.3639781563930313
1103	7	0.3502073714344007
1103	8	0.34128822577698514
1103	9	0.4442606353111529
1103	10-11	0.5377790618610447
1103	12-13	0.4668546547517849
1103	14-15	0.6556396423769613
1103	16-17	0.6092056994427324
1103	18-19	0.6428762589088208
1103	20-21	0.5219952729279882
1103	22-23	0.6410431301033483
1103	24-25	0.7025462252187253
1103	26-27	0.6142625546753848
1103	28-29	0.5662884210154147
1103	30-31	0.6196436083843011
1103	32-33	0.6180883739717586
1103	34-35	0.5298976954957482
1103	36-37	0.5650389554756643
1103	38-39	0.5954689680689569
1103	40-41	0.5808507474175215
1103	42-43	0.5706689841357004
1103	44-45	0.46823180492159366
1103	46-47	0.485174812281592
1103	48-49	0.5035776878510134
1103	50-51	0.49320745301069735
1103	52-53	0.48938882387152205
1103	54-55	0.4930037175696853
1103	56-57	0.5057631214847476
1103	58-59	0.5041514877923703
1103	60-61	0.5535095567658388
1103	62-63	0.5885459976391516
1103	64-65	0.5192930852164608
1103	66-67	0.6167248964372476
1103	68-69	0.5638681339237479
1103	70-71	0.609597868435614
1103	72-73	0.5233664499323396
1103	74-75	0.5547324065448862
1103	76-77	0.43980874966815975
1103	78-79	0.4861688669771169
1103	80-81	0.4266774250831702
1103	82-83	0.4895133012611126
1103	84-85	0.420926618271352
1103	86-87	0.4057024024530804
1103	88-89	0.5529877318625935
1103	90-91	0.5378983277287475
1103	92-93	0.4645318173841595
1103	94-95	0.41593114473333515
1103	96-97	0.4578014885023727
1103	98-99	0.48096017734027896
1103	100	0.5976853218632137
1104	1	0.21247774885838666
1104	2	0.3178051449415342
1104	3	0.24829456214181533
1104	4	0.4244231859785472
1104	5	0.28887972851386934
1104	6	0.3018582155889078
1104	7	0.2675139962559854
1104	8	0.40511728292242566
1104	9	0.38308480920927224
1104	10-11	0.5174430766514391
1104	12-13	0.39191489562307424
1104	14-15	0.5844963603381501
1104	16-17	0.5414732190580267
1104	18-19	0.4585565723004592
1104	20-21	0.4920443152898244
1104	22-23	0.5102770664843632
1104	24-25	0.5321486261668582
1104	26-27	0.508781126746733
1104	28-29	0.4579393237913578
1104	30-31	0.40415143448068847
1104	32-33	0.45043517002365263
1104	34-35	0.5102559301146812
1104	36-37	0.5060307755612286
1104	38-39	0.4959782331359932
1104	40-41	0.5508895885442797
1104	42-43	0.5894448880945404
1104	44-45	0.49253000443292194
1104	46-47	0.47262616078175057
1104	48-49	0.44839293893404175
1104	50-51	0.3867723056490533
1104	52-53	0.43101806444103374
1104	54-55	0.494985133082956
1104	56-57	0.49273525458180956
1104	58-59	0.4954730271633849
1104	60-61	0.5239983744732228
1104	62-63	0.5546954889847413
1104	64-65	0.4999204241706039
1104	66-67	0.46810963372339387
1104	68-69	0.41023546096721475
1104	70-71	0.453553740784173
1104	72-73	0.4688329422013666
1104	74-75	0.452584517794115
1104	76-77	0.4232608399366171
1104	78-79	0.43419117809937546
1104	80-81	0.4225492410340408
1104	82-83	0.4302461676953442
1104	84-85	0.41327073793974733
1104	86-87	0.4062085414330099
1104	88-89	0.3485577611070738
1104	90-91	0.29951707952786677
1104	92-93	0.3661966528836338
1104	94-95	0.49608152084650925
1104	96-97	0.41590884779670745
1104	98-99	0.3513199802601008
1104	100	0.5091432520506132
1105	1	0.16487827870357563
1105	2	0.35183434181315576
1105	3	0.31415832795344656
1105	4	0.4193557133082564
1105	5	0.2577252063219433
1105	6	0.3179786336783792
1105	7	0.3898928268638997
1105	8	0.34291301282825515
1105	9	0.47892819374781226
1105	10-11	0.4859648582258984
1105	12-13	0.37146355612352977
1105	14-15	0.5559334107839078
1105	16-17	0.5508037019938925
1105	18-19	0.4931821164512158
1105	20-21	0.44568544348261696
1105	22-23	0.5318589740445674
1105	24-25	0.4688612139834447
1105	26-27	0.5207644141800714
1105	28-29	0.483025466739889
1105	30-31	0.5739863796972315
1105	32-33	0.5417442758731923
1105	34-35	0.5437559690415981
1105	36-37	0.524797562855575
1105	38-39	0.5313105615063805
1105	40-41	0.5396193681280863
1105	42-43	0.549951243347536
1105	44-45	0.562208800782301
1105	46-47	0.5282091756189331
1105	48-49	0.37925575429463976
1105	50-51	0.5058615834492102
1105	52-53	0.46358147855630705
1105	54-55	0.3692972912944512
1105	56-57	0.4992106228742159
1105	58-59	0.5265011410962472
1105	60-61	0.4529951888102346
1105	62-63	0.3963919069341628
1105	64-65	0.46636954338237757
1105	66-67	0.42647568996036966
1105	68-69	0.44319854436740513
1105	70-71	0.5108975350363423
1105	72-73	0.48744731574149114
1105	74-75	0.35230659651845997
1105	76-77	0.35434662344405865
1105	78-79	0.44954929359195717
1105	80-81	0.4015821733236038
1105	82-83	0.3792588159020127
1105	84-85	0.3687501028843414
1105	86-87	0.3327042533830564
1105	88-89	0.3653015791037362
1105	90-91	0.3724275313844245
1105	92-93	0.44946046370774795
1105	94-95	0.4360042725296296
1105	96-97	0.29336243525679606
1105	98-99	0.29105456317476097
1105	100	0.4479282277387284
1106	1	0.11538855388987557
1106	2	0.2257184379842201
1106	3	0.17556831622976077
1106	4	0.33948067752463373
1106	5	0.22041533825764503
1106	6	0.2676724314778127
1106	7	0.2812893611828571
1106	8	0.2349103250200102
1106	9	0.3707480643152081
1106	10-11	0.4186815461359714
1106	12-13	0.2437634168672247
1106	14-15	0.32497521714326894
1106	16-17	0.28544115366206313
1106	18-19	0.2830281822642249
1106	20-21	0.27915093585379935
1106	22-23	0.3070774743667357
1106	24-25	0.3710031156005158
1106	26-27	0.2975007630172044
1106	28-29	0.3108352416589213
1106	30-31	0.36979529439830827
1106	32-33	0.26202314645997404
1106	34-35	0.2855453985842118
1106	36-37	0.32325996859311346
1106	38-39	0.2954341125593558
1106	40-41	0.3521553457629025
1106	42-43	0.2766409308628184
1106	44-45	0.26990854576867207
1106	46-47	0.3345615253003942
1106	48-49	0.19785772392690149
1106	50-51	0.2725193770268106
1106	52-53	0.2533791374556529
1106	54-55	0.3812199453712495
1106	56-57	0.28244833365593536
1106	58-59	0.238538933682932
1106	60-61	0.21336033557439293
1106	62-63	0.2773388167145967
1106	64-65	0.25184608651878904
1106	66-67	0.2773405399940714
1106	68-69	0.23932146402410126
1106	70-71	0.23285445149235784
1106	72-73	0.21872121759996332
1106	74-75	0.22631616969869128
1106	76-77	0.24559330092371567
1106	78-79	0.2682783124754309
1106	80-81	0.20969889100953054
1106	82-83	0.23683843958063733
1106	84-85	0.2182125230945573
1106	86-87	0.26888715044542977
1106	88-89	0.3228344245502157
1106	90-91	0.18432085508435136
1106	92-93	0.2455863565144618
1106	94-95	0.23479441010524127
1106	96-97	0.32744051948873576
1106	98-99	0.21645775557594504
1106	100	0.23162525492331554
1107	1	0.010881051270384745
1107	2	0.08136548687001266
1107	3	0.03767849523384825
1107	4	0.15728661088540719
1107	5	0.06415041484345352
1107	6	0.1340218036684817
1107	7	0.056527731428708705
1107	8	0.07355053776646514
1107	9	0.11533881155378367
1107	10-11	0.24331728852297374
1107	12-13	0.12496747841225186
1107	14-15	0.26551133876996147
1107	16-17	0.14385706563356138
1107	18-19	0.125304850506474
1107	20-21	0.12434726642874949
1107	22-23	0.17729399904067833
1107	24-25	0.27223395217438906
1107	26-27	0.1405138244306201
1107	28-29	0.22391619518383976
1107	30-31	0.29341519123216386
1107	32-33	0.2936898700395574
1107	34-35	0.2273640776415249
1107	36-37	0.3096880663179675
1107	38-39	0.3057007126079654
1107	40-41	0.2584767926848244
1107	42-43	0.3407359326910182
1107	44-45	0.333900165791718
1107	46-47	0.27365644225846353
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2209	7	0.06791172922776667
2209	8	0.14070124818891117
2209	9	0.23028912498174492
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2212	98-99	-0.006130487887485714
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2214	3	-0.6628330860749614
2214	4	-0.6211784999471277
2214	5	-0.5531847691028133
2214	6	-0.5718138905882313
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2214	14-15	-3.2451931982878364
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2214	84-85	-0.6178345493883057
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2214	88-89	-0.7526253421873719
2214	90-91	-0.7284539544504014
2214	92-93	-0.7162135967890926
2214	94-95	-0.6983741209516872
2214	96-97	-0.6598757014669694
2214	98-99	-0.5852271064414118
2214	100	-0.5596326708290107
2215	1	-0.858933728871559
2215	2	-1.230668039167167
2215	3	-0.8760596157332046
2215	4	-2.403987227798538
2215	5	-1.1039035460871602
2215	6	-0.8851030547422027
2215	7	-0.7987415641932927
2215	8	-0.7210751863181528
2215	9	-1.06786282137071
2215	10-11	-0.7716737560633362
2215	12-13	-0.9563993197246674
2215	14-15	-0.8788782666340964
2215	16-17	-0.9641366915369005
2215	18-19	-0.8927343533411332
2215	20-21	-1.043745811209348
2215	22-23	-0.9357509625850184
2215	24-25	-1.0492791557229566
2215	26-27	-1.0491020492877112
2215	28-29	-0.8816751495541411
2215	30-31	-3.6482549037666203
2215	32-33	-1.393799249488751
2215	34-35	-1.2109450528981256
2215	36-37	-1.063715462985357
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2215	40-41	-1.0116464463541206
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2215	46-47	-1.0623545977738544
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2215	52-53	-1.0061958314646517
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2215	56-57	-0.8984302502369843
2215	58-59	-0.9030534696527752
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2215	62-63	-0.8662259488633097
2215	64-65	-0.9189697835660482
2215	66-67	-0.8931759401313002
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2215	70-71	-0.9017828522232563
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2215	84-85	-0.8413656591481242
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2215	88-89	-0.8055067366200959
2215	90-91	-0.886363466790776
2215	92-93	-0.9100508268037935
2215	94-95	-0.9265356699023961
2215	96-97	-1.1747755107327649
2215	98-99	-0.9657385164793801
2215	100	-0.45352532592613315
2216	1	-0.5602476684095343
2216	2	-0.8144919291885451
2216	3	-0.7335783814853301
2216	4	-0.5757756319480336
2216	5	-0.5594461092875846
2216	6	-0.45272211242417626
2216	7	-0.5956666381864224
2216	8	-0.6660335059717397
2216	9	-0.7642495514460634
2216	10-11	-1.2014638286313186
2216	12-13	-0.7702925226675603
2216	14-15	-0.9071919577928114
2216	16-17	-0.9098679423229683
2216	18-19	-1.2973693904969537
2216	20-21	-1.283881566347624
2216	22-23	-1.110933206908534
2216	24-25	-1.1624217643115458
2216	26-27	-1.6054866671695507
2216	28-29	-1.3969879655154216
2216	30-31	-1.72451315449063
2216	32-33	-1.9617830535890803
2216	34-35	-1.4332504723027881
2216	36-37	-1.5368816727076933
2216	38-39	-1.349909851965002
2216	40-41	-1.2306323453951507
2216	42-43	-1.2909741610255878
2216	44-45	-1.1759612129052357
2216	46-47	-1.2985599783239579
2216	48-49	-1.2084518820414587
2216	50-51	-1.3511128992813255
2216	52-53	-1.4053038048777609
2216	54-55	-1.4317672572463849
2216	56-57	-1.3817846289396982
2216	58-59	-1.2649486819869225
2216	60-61	-1.3524530564203516
2216	62-63	-1.4318894154904527
2216	64-65	-1.3456306909578473
2216	66-67	-1.392100089414484
2216	68-69	-1.3214114713394167
2216	70-71	-1.272516060540049
2216	72-73	-1.2756528978322308
2216	74-75	-1.3666783532174236
2216	76-77	-1.2239221501620605
2216	78-79	-1.172477496916489
2216	80-81	-1.3307636827266762
2216	82-83	-1.2470988945509944
2216	84-85	-1.2418050597139327
2216	86-87	-1.1762116821535926
2216	88-89	-1.243971273513754
2216	90-91	-1.252450780929994
2216	92-93	-1.255593217986526
2216	94-95	-1.2099221730368939
2216	96-97	-1.2497404257590894
2216	98-99	-1.2534590360156912
2216	100	-0.8640347545374638
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	17.0
3	19.0
4	26.0
5	29.0
6	48.0
7	148.0
8	424.0
9	747.0
10	1324.0
11	2848.0
12	5412.0
13	7999.0
14	10479.0
15	13140.0
16	15909.0
17	18007.0
18	20674.0
19	22849.0
20	25695.0
21	28810.0
22	33222.0
23	37851.0
24	43743.0
25	49793.0
26	56018.0
27	62038.0
28	71200.0
29	84683.0
30	98297.0
31	114791.0
32	142378.0
33	183027.0
34	259694.0
35	365981.0
36	585702.0
37	970254.0
38	835325.0
39	282777.0
40	273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60347757603573	20.24221931582618	11.707094615627875	24.44720849251021
2	15.956774044117827	19.8379487193312	39.86314581312859	24.342131423422376
3	18.729910327158947	26.37137711927234	28.725457687341116	26.1732548662276
4	12.058916762089751	16.26375547068012	40.33816278605302	31.33916498117711
5	13.018584788464088	38.76681740271235	35.214209174905484	13.000388633918082
6	30.123745439237226	38.44067627558711	18.118285777698503	13.317292507477157
7	27.115101269897536	31.737105084689414	23.68673060295591	17.461063042457138
8	26.50704255093076	34.766733541753574	19.830563644438396	18.89566026287727
9	26.393853378188954	14.694618763163758	21.073380992430614	37.83814686621667
10-11	25.828352285407785	25.54651128964523	27.78387480432701	20.84126162061998
12-13	25.890565650343905	23.50920571466182	27.612033505876603	22.98819512911767
14-15	23.22998236494005	24.80104258123994	24.969104107280828	26.999870946539183
16-17	22.321363286287223	27.363016048856757	26.276738687924222	24.038881976931798
18-19	21.66180135839257	28.257306105578127	27.693563079217853	22.38732945681145
20-21	23.395076738874423	27.335258099715325	26.778281401893356	22.491383759516896
22-23	23.796277659668146	27.09312005908876	26.367719260096578	22.742883021146515
24-25	23.284502135639503	26.95398547127164	27.3625919165135	22.398920476575356
26-27	23.21473480021633	27.223268997377854	26.157505246823966	23.404490955581853
28-29	22.31619485098586	27.797514688113946	26.757353492051568	23.128936968848627
30-31	23.155507326256867	27.50525366003953	26.780316530419896	22.55892248328371
32-33	23.54457229616745	27.530391976152117	25.85455231247924	23.070483415201195
34-35	23.4573614381385	27.303117406710477	26.91042994565096	22.329091209500067
36-37	23.06141074596789	26.871165092874605	26.537633128318205	23.529791032839302
38-39	23.294452559270304	27.100896215246216	27.090304008684036	22.51434721679944
40-41	22.875998914946276	26.211370936173346	27.712114804524653	23.200515344355725
42-43	22.866590129902807	26.46633805285081	27.01310036800554	23.65397144924085
44-45	23.068575304002167	27.36977221476967	26.930655162686644	22.630997318541517
46-47	22.936232056310352	27.119369888808464	26.49275691845134	23.451641136429842
48-49	23.692272848959696	26.82164190514751	26.67886627454016	22.80721897135264
50-51	23.1696469594902	26.518738335090674	26.80276279873795	23.508851906681176
52-53	22.647796807518404	27.178594226875564	26.58497806660302	23.588630899003014
54-55	22.66300726731009	27.015754282664446	26.62490508774616	23.6963333622793
56-57	23.77793794221993	26.508774133163005	26.26945610417119	23.443831820445872
58-59	22.633127785688835	26.931856529751236	27.22896671530131	23.20604896925862
60-61	22.680211672164944	27.531704261538493	26.500596110102148	23.287487956194415
62-63	23.157902540811687	26.826786898563437	26.05280042847104	23.962510132153838
64-65	22.271829703928105	27.045837769905432	26.657108121457178	24.025224404709284
66-67	22.974612582420278	26.658939639659778	26.524521721704136	23.841926056215808
68-69	22.42874142213658	26.673838895330015	26.89174644816305	24.005673234370356
70-71	22.366955774323944	26.404623116526178	26.95920479556119	24.26921631358869
72-73	22.939470022344125	26.339670234328	27.50855820862394	23.212301534703936
74-75	23.134273031198475	26.575397307705185	26.940831205979045	23.3494984551173
76-77	22.812212682331754	26.546897264047782	26.69235266729084	23.94853738632962
78-79	22.70068437946693	26.498510203909696	26.630847906001026	24.169957510622346
80-81	22.254299710830818	26.916929489022117	27.338186630775223	23.490584169371836
82-83	23.070513644948836	25.892137762004864	28.338057531693877	22.699291061352426
84-85	23.219976615213323	26.371519760638996	27.117793027900778	23.290710596246907
86-87	21.926755838642144	27.05876485453858	27.569722565509142	23.444756741310137
88-89	23.14670298262071	25.787738355742313	27.25932976711204	23.806228894524942
90-91	22.7299692130864	25.83014974232655	27.403678141448605	24.03620290313844
92-93	22.999270993751214	26.067398818088787	27.350730634999266	23.58259955316073
94-95	21.829621871676565	26.143283402368635	28.030375531356132	23.99671919459867
96-97	22.168531838978875	25.941164286282348	27.648665248935018	24.241638625803763
98-99	21.56058322910237	26.59407661102128	27.51991714691539	24.325423012960957
100	22.397271303304006	25.89955666561191	26.59121236246443	25.111959668619647
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	180.0
1	136.0
2	112.5
3	132.5
4	127.0
5	116.5
6	106.5
7	113.0
8	119.0
9	108.5
10	109.5
11	111.5
12	119.0
13	127.5
14	148.5
15	206.5
16	238.0
17	274.5
18	350.0
19	462.0
20	656.5
21	941.5
22	1377.5
23	2021.0
24	2940.0
25	4247.0
26	6144.5
27	8645.5
28	12554.0
29	20403.5
30	32253.5
31	44123.0
32	59259.5
33	74699.0
34	86129.5
35	98937.0
36	111855.0
37	131939.5
38	141426.5
39	135010.0
40	127892.5
41	119028.5
42	118499.5
43	127235.0
44	139702.0
45	164859.0
46	188766.5
47	205510.0
48	217856.0
49	214662.5
50	203707.5
51	207544.0
52	210328.0
53	200307.0
54	188546.5
55	162372.5
56	141790.5
57	117317.0
58	91859.5
59	87470.5
60	72701.0
61	49615.5
62	36315.5
63	30280.5
64	22248.0
65	10928.0
66	5818.5
67	3713.0
68	2302.0
69	1379.0
70	829.0
71	459.5
72	261.0
73	158.5
74	92.5
75	68.0
76	49.5
77	30.0
78	28.5
79	28.0
80	18.5
81	18.5
82	17.5
83	12.5
84	14.0
85	13.0
86	10.5
87	9.5
88	9.5
89	8.0
90	7.0
91	8.0
92	6.5
93	7.5
94	6.5
95	5.0
96	5.0
97	6.0
98	7.5
99	6.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05528284780571348
2	0.007749931935380394
3	0.008086885497788236
4	0.009502090459901177
5	0.003639098474004706
6	0.0021115756577558173
7	7.41297837297255E-4
8	0.0015499863870760786
9	8.086885497788237E-4
10-11	6.402117685749021E-4
12-13	3.369535624078432E-4
14-15	1.9094035203111114E-4
16-17	5.95284626920523E-4
18-19	0.0015050592454216997
20-21	6.626753394020917E-4
22-23	9.8839711639634E-4
24-25	0.0013028871079769936
26-27	0.003358303838664837
28-29	0.004009747392653334
30-31	0.0052003166464943806
32-33	0.006098859479581962
34-35	0.0033133766970104583
36-37	0.007963335858238694
38-39	0.005975309840032419
40-41	0.005829296629655687
42-43	0.014196976762783793
44-45	0.020273372671538566
46-47	0.018386432722054646
48-49	0.02162118692116994
50-51	0.018038247374233204
52-53	0.025451225747205753
54-55	0.01865599557198092
56-57	0.015073056025044187
58-59	0.017476658103553467
60-61	0.01600529421437255
62-63	0.010692659713742225
64-65	0.010928527207427714
66-67	0.01366908284834484
68-69	0.005009376294463269
70-71	0.008289057635232943
72-73	0.008311521206060131
74-75	0.011344103267730722
76-77	0.010322010795093596
78-79	0.01211909646126876
80-81	0.01014230222847608
82-83	0.006278568046199477
84-85	0.010501719361711112
86-87	0.009052819043357387
88-89	0.007828554433275558
90-91	0.012860394298566015
92-93	0.008693401910122356
94-95	0.006873852673120001
96-97	0.009210064039147713
98-99	0.008974196545462224
100	0.25691585955056684
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4451652.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.94136500840261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.62256165699584	53.590865527307805
2	14.447744471622226	20.20989939275722
3	4.7933435407189275	10.057589705762746
4	1.9596088162956464	5.482308619768704
5	0.9297843798325705	3.2515194344490537
6	0.46713472663657335	1.9603224254273368
7	0.2632841181161425	1.2890115424253559
8	0.16684480807709995	0.9335482897181863
9	0.0979699949695932	0.6166939660235716
>10	0.24873809389799184	2.431394731911182
>50	0.0025428868186855905	0.11328207820501858
>100	3.9898869897154007E-4	0.041041196719830926
>500	4.351731977377233E-5	0.02252308952398743
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.2463570827189548E-5	0.0	0.0	0.0
4	0.0	2.2463570827189548E-5	0.0	0.0	0.0
5	0.0	2.2463570827189548E-5	0.0	0.0	0.0
6	0.0	2.2463570827189548E-5	0.0	0.0	0.0
7	0.0	2.2463570827189548E-5	0.0	0.0	0.0
8	0.0	2.2463570827189548E-5	0.0	2.2463570827189548E-5	0.0
9	0.0	2.2463570827189548E-5	0.0	4.4927141654379096E-5	0.0
10-11	0.0	2.2463570827189548E-5	0.0	4.4927141654379096E-5	0.0
12-13	0.0	2.2463570827189548E-5	0.0	4.4927141654379096E-5	0.0
14-15	0.0	2.2463570827189548E-5	0.0	4.4927141654379096E-5	0.0
16-17	0.0	2.2463570827189548E-5	0.0	4.4927141654379096E-5	0.0
18-19	0.0	2.2463570827189548E-5	0.0	1.235496395495425E-4	0.0
20-21	0.0	2.2463570827189548E-5	0.0	1.6847678120392162E-4	0.0
22-23	0.0	2.2463570827189548E-5	0.0	1.9094035203111114E-4	0.0
24-25	0.0	2.2463570827189548E-5	0.0	3.3695356240784325E-4	0.0
26-27	0.0	2.2463570827189548E-5	0.0	8.648474768467976E-4	2.2463570827189548E-5
28-29	0.0	2.2463570827189548E-5	0.0	0.0035604759761095435	2.2463570827189548E-5
30-31	0.0	2.2463570827189548E-5	0.0	0.011950619680064839	2.2463570827189548E-5
32-33	0.0	2.2463570827189548E-5	0.0	0.02683273535307791	2.2463570827189548E-5
34-35	0.0	2.2463570827189548E-5	0.0	0.05260968287727792	4.4927141654379096E-5
36-37	0.0	2.2463570827189548E-5	0.0	0.09629009635074799	4.4927141654379096E-5
38-39	0.0	2.2463570827189548E-5	0.0	0.16446703381126826	4.4927141654379096E-5
40-41	0.0	2.2463570827189548E-5	0.0	0.23932688359287746	4.4927141654379096E-5
42-43	0.0	2.2463570827189548E-5	0.0	0.3198138578666976	4.4927141654379096E-5
44-45	0.0	3.3695356240784324E-5	0.0	0.41509309353022206	4.4927141654379096E-5
46-47	0.0	4.4927141654379096E-5	0.0	0.5359358727950883	4.4927141654379096E-5
48-49	0.0	4.4927141654379096E-5	0.0	0.676771230096153	4.4927141654379096E-5
50-51	0.0	4.4927141654379096E-5	0.0	0.8299166242105178	4.4927141654379096E-5
52-53	0.0	4.4927141654379096E-5	0.0	0.9810402969504355	4.4927141654379096E-5
54-55	0.0	4.4927141654379096E-5	0.0	1.145945370392834	4.4927141654379096E-5
56-57	0.0	4.4927141654379096E-5	0.0	1.3502403152807094	5.615892706797386E-5
58-59	0.0	4.4927141654379096E-5	0.0	1.5965870647570832	6.739071248156863E-5
60-61	0.0	4.4927141654379096E-5	0.0	1.8476287005363403	6.739071248156863E-5
62-63	0.0	4.4927141654379096E-5	0.0	2.1128785448637943	8.985428330875819E-5
64-65	0.0	4.4927141654379096E-5	0.0	2.396582212625785	8.985428330875819E-5
66-67	0.0	4.4927141654379096E-5	0.0	2.7150370244574376	8.985428330875819E-5
68-69	0.0	4.4927141654379096E-5	0.0	3.0734994559323146	8.985428330875819E-5
70-71	0.0	4.4927141654379096E-5	0.0	3.469285110336567	8.985428330875819E-5
72-73	0.0	4.4927141654379096E-5	0.0	3.8808626550323337	8.985428330875819E-5
74-75	0.0	4.4927141654379096E-5	0.0	4.284117446736627	8.985428330875819E-5
76-77	0.0	4.4927141654379096E-5	0.0	4.7462492575789845	8.985428330875819E-5
78-79	0.0	4.4927141654379096E-5	0.0	5.247164423454484	8.985428330875819E-5
80-81	0.0	4.4927141654379096E-5	0.0	5.784526732997099	8.985428330875819E-5
82-83	0.0	4.4927141654379096E-5	0.0	6.350979366760924	8.985428330875819E-5
84-85	0.0	4.4927141654379096E-5	0.0	6.90071910382932	8.985428330875819E-5
86-87	0.0	5.615892706797386E-5	0.0	7.48368246215113	8.985428330875819E-5
88	0.0	6.739071248156863E-5	0.0	7.962841659680496	8.985428330875819E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4610	0.0	42.82622	94
GTACTAG	4950	0.0	34.86132	1
CTGTGCG	6225	0.0	32.837814	9
TAGGCAT	6135	0.0	32.247917	5
TAGTACT	6580	0.0	31.99782	4
GCATCGT	5075	0.0	31.852737	8
ACTGTGC	7065	0.0	30.729408	8
CAACGCA	7340	0.0	30.283022	5
CTAACGC	2565	0.0	29.315742	3
CGCCTAC	2590	0.0	29.211273	7
TCTAACG	2595	0.0	29.158264	2
GTATCAA	8755	0.0	29.055216	1
TAACGCC	2630	0.0	28.948597	4
CTAGGCA	7000	0.0	28.9366	4
GTACTGT	7255	0.0	28.88829	6
GGTATCA	5950	0.0	28.765202	1
TACTGTG	7995	0.0	28.447968	7
CTAGTAC	7820	0.0	28.306257	3
ATCAACG	7950	0.0	27.843386	3
ACGCCTA	2935	0.0	26.0978	6
>>END_MODULE