FastQCFastQC Report
Mon 16 Jan 2017
ERR1146649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4451652
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65060.1461479918016952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55450.12456050023676604No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC43750.040.74326394
GGTATCA74700.039.9291651
GTATCAA125200.037.088091
CAACGCA136750.032.270525
CTGTGCG61350.031.6446849
ATCAACG139350.031.6325843
TAACGCC22850.030.4703584
CTAACGC22000.030.1507763
TAGTACT64450.030.0729714
ACTGTGC70600.029.2920538
GTACTAG47750.029.0649931
TCTAACG23350.028.8118232
TATCAAC160650.028.757582
GTGGTAT20900.028.5876871
CGCCTAC23350.028.5807787
AACGCAG163750.028.212396
TACTGTG77300.027.9673257
GTACTGT71400.027.7109626
CTAGTAC78850.026.609413
ACGCAGA169350.026.0309627
Produced by FastQC (version 0.11.5)