##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146649_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4451652
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06852377499409	33.0	31.0	34.0	30.0	34.0
2	32.13233446819293	33.0	31.0	34.0	30.0	34.0
3	32.29611434137259	34.0	31.0	34.0	30.0	34.0
4	35.92048772006437	37.0	35.0	37.0	35.0	37.0
5	35.82289428733423	37.0	35.0	37.0	35.0	37.0
6	35.856620194031336	37.0	35.0	37.0	35.0	37.0
7	35.77838923617569	37.0	35.0	37.0	33.0	37.0
8	35.781486962592766	37.0	35.0	37.0	33.0	37.0
9	37.48783979520412	39.0	37.0	39.0	34.0	39.0
10-11	37.41941485992166	39.0	37.0	39.0	34.0	39.0
12-13	37.39538209635434	39.0	37.0	39.0	34.0	39.0
14-15	38.61040586730499	40.0	38.0	41.0	34.0	41.0
16-17	38.605370545586226	40.0	38.0	41.0	34.0	41.0
18-19	38.64829371208711	40.0	38.0	41.0	34.0	41.0
20-21	38.64254023000899	40.0	38.0	41.0	34.0	41.0
22-23	38.607251869642994	40.0	38.0	41.0	34.0	41.0
24-25	38.538051491895594	40.0	38.0	41.0	34.0	41.0
26-27	38.3987362444324	40.0	38.0	41.0	34.0	41.0
28-29	38.341513891921466	40.0	38.0	41.0	34.0	41.0
30-31	38.22440736607444	40.0	37.5	41.0	33.5	41.0
32-33	38.100536497462066	40.0	37.0	41.0	33.0	41.0
34-35	38.01265867143254	40.0	37.0	41.0	33.0	41.0
36-37	38.018233568122575	40.0	37.0	41.0	33.0	41.0
38-39	37.93127124492211	40.0	37.0	41.0	33.0	41.0
40-41	37.82520758585802	40.0	37.0	41.0	33.0	41.0
42-43	37.703729199856596	40.0	37.0	41.0	33.0	41.0
44-45	37.518628926969136	40.0	36.0	41.0	32.0	41.0
46-47	37.31220926523457	40.0	36.0	41.0	31.5	41.0
48-49	37.08020224851359	39.5	35.5	41.0	31.0	41.0
50-51	36.88507131734466	39.0	35.0	41.0	31.0	41.0
52-53	36.70638630333188	39.0	35.0	41.0	31.0	41.0
54-55	36.52145596735774	39.0	35.0	41.0	30.5	41.0
56-57	36.30218017940307	38.0	35.0	40.5	30.0	41.0
58-59	36.03598562960447	38.0	35.0	40.0	30.0	41.0
60-61	35.85754456997088	37.0	35.0	40.0	30.0	41.0
62-63	35.64365532166485	37.0	35.0	40.0	30.0	41.0
64-65	35.396275135612576	36.0	34.0	40.0	29.5	41.0
66-67	35.14331320148116	36.0	34.0	39.0	29.0	41.0
68-69	34.88231121839713	35.0	34.0	39.0	29.0	41.0
70-71	34.657198271563004	35.0	34.0	38.5	29.0	41.0
72-73	34.409422951299874	35.0	34.0	37.5	29.0	40.0
74-75	34.1439483589463	35.0	34.0	37.0	29.0	39.5
76-77	33.89314719569275	35.0	34.0	36.5	29.0	39.0
78-79	33.66156114628906	35.0	33.5	36.0	29.0	39.0
80-81	33.52459805932719	35.0	33.0	36.0	29.0	38.0
82-83	33.36021470231725	35.0	33.0	35.5	29.0	37.0
84-85	33.2352376151595	35.0	33.0	35.0	29.0	37.0
86-87	33.09047191918865	35.0	33.0	35.0	29.0	36.5
88-89	32.98663293986142	35.0	33.0	35.0	29.0	36.0
90-91	32.92358589575286	35.0	33.0	35.0	29.0	36.0
92-93	32.85951923016444	35.0	33.0	35.0	29.0	36.0
94-95	32.81907357089008	35.0	33.0	35.0	29.0	35.5
96-97	32.72873238968365	35.0	33.0	35.0	29.0	35.0
98-99	32.65120588940914	35.0	33.0	35.0	28.0	35.0
100	31.391303273481395	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4072056523059615
1101	2	0.4774742850008664
1101	3	0.4154697886880996
1101	4	0.303693298900086
1101	5	0.25737265933818776
1101	6	0.27742120711778284
1101	7	0.3195784450481227
1101	8	0.2688576531882916
1101	9	0.38448263937842597
1101	10-11	0.416541810692614
1101	12-13	0.41744622786006147
1101	14-15	0.5320944700680172
1101	16-17	0.5550498595828728
1101	18-19	0.5541709090193336
1101	20-21	0.5693225735565619
1101	22-23	0.5730143007446316
1101	24-25	0.588423557531975
1101	26-27	0.598792386729599
1101	28-29	0.6261823828469986
1101	30-31	0.5795838810880412
1101	32-33	0.6097951645532191
1101	34-35	0.7147459055691954
1101	36-37	0.6352768511358562
1101	38-39	0.6902999242140808
1101	40-41	0.6974453839604067
1101	42-43	0.6480823912889733
1101	44-45	0.6597100256405568
1101	46-47	0.6662531687047348
1101	48-49	0.6640647959002592
1101	50-51	0.6769666896861324
1101	52-53	0.6936731710070916
1101	54-55	0.705807494239231
1101	56-57	0.7582833603329107
1101	58-59	0.6589222576997003
1101	60-61	0.6382865605679697
1101	62-63	0.6499502190928013
1101	64-65	0.6687772473852647
1101	66-67	0.558184076852875
1101	68-69	0.5787635347350744
1101	70-71	0.5844098593013527
1101	72-73	0.6270131278800761
1101	74-75	0.6261264980867054
1101	76-77	0.5744296013379753
1101	78-79	0.5235589913855563
1101	80-81	0.6224729288699535
1101	82-83	0.5805668877520489
1101	84-85	0.5467691639380163
1101	86-87	0.5263876632398308
1101	88-89	0.5272213203267739
1101	90-91	0.542560018341625
1101	92-93	0.5476196255730272
1101	94-95	0.5671438069915453
1101	96-97	0.5450884806136642
1101	98-99	0.5864622567011324
1101	100	0.9094987784288371
1102	1	0.36970181018278225
1102	2	0.3963569628958439
1102	3	0.30918330513728165
1102	4	0.23023883955204383
1102	5	0.22108619772495075
1102	6	0.1903005446950914
1102	7	0.24442114208243026
1102	8	0.26434243412594327
1102	9	0.3177030096922877
1102	10-11	0.3032442320672786
1102	12-13	0.35987974973912884
1102	14-15	0.510854495181448
1102	16-17	0.4844618662147582
1102	18-19	0.4499545130191791
1102	20-21	0.46835153211343084
1102	22-23	0.5348024066458805
1102	24-25	0.5533276970711043
1102	26-27	0.5279072388848363
1102	28-29	0.547324587041409
1102	30-31	0.5916944217556264
1102	32-33	0.6079974242878166
1102	34-35	0.6031254991624806
1102	36-37	0.5880011361220241
1102	38-39	0.5890205933111261
1102	40-41	0.5039844466004624
1102	42-43	0.5408184334878356
1102	44-45	0.5932120279321325
1102	46-47	0.5932218608769162
1102	48-49	0.6419328590023952
1102	50-51	0.6073268761449171
1102	52-53	0.6588766477011774
1102	54-55	0.6230647304991734
1102	56-57	0.5690144283741319
1102	58-59	0.5548821822193588
1102	60-61	0.5260780940940322
1102	62-63	0.5297838515860178
1102	64-65	0.5532237765644794
1102	66-67	0.5284216739053349
1102	68-69	0.558783574602522
1102	70-71	0.4979426708172454
1102	72-73	0.4840413065671072
1102	74-75	0.5674779322714301
1102	76-77	0.5365394805543175
1102	78-79	0.5081728156544258
1102	80-81	0.43307222740368445
1102	82-83	0.4330090820656878
1102	84-85	0.4243328220829099
1102	86-87	0.49505806403695374
1102	88-89	0.48259345999611725
1102	90-91	0.45422265126368444
1102	92-93	0.43571214711224115
1102	94-95	0.45108619288228624
1102	96-97	0.4591271416741449
1102	98-99	0.45513728433306255
1102	100	0.6789535052823759
1103	1	0.2949811994483369
1103	2	0.37466857966213496
1103	3	0.2760048199332843
1103	4	0.23671134605498878
1103	5	0.2206020624556757
1103	6	0.22189829379802717
1103	7	0.23690877815700873
1103	8	0.1973510147816242
1103	9	0.3039078476378734
1103	10-11	0.34289832399360165
1103	12-13	0.3315723668077979
1103	14-15	0.4621272824202194
1103	16-17	0.4435299202264744
1103	18-19	0.4285758698856199
1103	20-21	0.43273138928037724
1103	22-23	0.40667441055002485
1103	24-25	0.4152175582389006
1103	26-27	0.4431782118376404
1103	28-29	0.48319670568498196
1103	30-31	0.4509675725029467
1103	32-33	0.4680876550769142
1103	34-35	0.4443793919732997
1103	36-37	0.44080403507140886
1103	38-39	0.4232028857097845
1103	40-41	0.41956948117135795
1103	42-43	0.4343499478754822
1103	44-45	0.45655977509351686
1103	46-47	0.43395209845017035
1103	48-49	0.42184017434267673
1103	50-51	0.42047449318477703
1103	52-53	0.43252389985460127
1103	54-55	0.37265183764500875
1103	56-57	0.3235910724361304
1103	58-59	0.34730226482673743
1103	60-61	0.4173874714372019
1103	62-63	0.4631710661059145
1103	64-65	0.462972256705406
1103	66-67	0.4526125103833536
1103	68-69	0.39259045861774666
1103	70-71	0.4724200990906908
1103	72-73	0.43702243994718515
1103	74-75	0.4025014175292938
1103	76-77	0.3760833048797352
1103	78-79	0.3551570099218111
1103	80-81	0.31023066903174623
1103	82-83	0.32953850859649236
1103	84-85	0.3494310833796632
1103	86-87	0.33397993424961925
1103	88-89	0.3671285761497529
1103	90-91	0.33221307903884423
1103	92-93	0.31197309088666003
1103	94-95	0.2856187161251569
1103	96-97	0.30005591854928326
1103	98-99	0.2928836077233967
1103	100	0.6425606875700147
1104	1	0.195399047699091
1104	2	0.25959949718123454
1104	3	0.1985746754433535
1104	4	0.20209201408969335
1104	5	0.1809782815790939
1104	6	0.21094131805800487
1104	7	0.22532585705872066
1104	8	0.2475571464579147
1104	9	0.27389389307621315
1104	10-11	0.27583092571740764
1104	12-13	0.28340055754586757
1104	14-15	0.3526267094120925
1104	16-17	0.3164806750935938
1104	18-19	0.35632850888143963
1104	20-21	0.3705062615091492
1104	22-23	0.3736175155220849
1104	24-25	0.3537388494803011
1104	26-27	0.3808777324261001
1104	28-29	0.4351177893347895
1104	30-31	0.38685203151477054
1104	32-33	0.3986563101371843
1104	34-35	0.4680207896650401
1104	36-37	0.3945490065398971
1104	38-39	0.34568120308066597
1104	40-41	0.3972202837345691
1104	42-43	0.4490060188458287
1104	44-45	0.3621914250552365
1104	46-47	0.4361043972888936
1104	48-49	0.46393545464674446
1104	50-51	0.4554277159217577
1104	52-53	0.402800544004549
1104	54-55	0.3827044622681086
1104	56-57	0.340505842441452
1104	58-59	0.34825412397503896
1104	60-61	0.3584588490241174
1104	62-63	0.3825387549343162
1104	64-65	0.36945552275709304
1104	66-67	0.3245094341923149
1104	68-69	0.34808765650326734
1104	70-71	0.342119591936644
1104	72-73	0.3167663984502198
1104	74-75	0.3815568765792108
1104	76-77	0.3224570903463686
1104	78-79	0.31681787694874686
1104	80-81	0.2726860090557963
1104	82-83	0.27335199637228413
1104	84-85	0.2827445480636612
1104	86-87	0.3265557806852257
1104	88-89	0.34317544933875865
1104	90-91	0.29071737657957186
1104	92-93	0.259091394640258
1104	94-95	0.20391236351984787
1104	96-97	0.26183842190851436
1104	98-99	0.31146256415060236
1104	100	0.6065953587473096
1105	1	0.23179372799548048
1105	2	0.19366113077406055
1105	3	0.2063069082257556
1105	4	0.1997096545421897
1105	5	0.15167502938555089
1105	6	0.1873172674847723
1105	7	0.2422472606649606
1105	8	0.2081721798747722
1105	9	0.2280444768487513
1105	10-11	0.23595394899223976
1105	12-13	0.23444002809268483
1105	14-15	0.33051189252808655
1105	16-17	0.31440675210487967
1105	18-19	0.30301941942003907
1105	20-21	0.3764691446528019
1105	22-23	0.33306655118688866
1105	24-25	0.3496301337416483
1105	26-27	0.3907851246009386
1105	28-29	0.3761273633218778
1105	30-31	0.4407993535719612
1105	32-33	0.44565216408048514
1105	34-35	0.3460770580334511
1105	36-37	0.40025730422242134
1105	38-39	0.39695478814841323
1105	40-41	0.5094622272311469
1105	42-43	0.41310875718379947
1105	44-45	0.381618699061427
1105	46-47	0.40536995839059387
1105	48-49	0.3605189405310725
1105	50-51	0.3873684682060414
1105	52-53	0.36871080658420397
1105	54-55	0.3427068619307647
1105	56-57	0.29699953352662334
1105	58-59	0.3386444632040124
1105	60-61	0.3428439419748486
1105	62-63	0.4016749564887334
1105	64-65	0.33682659675334037
1105	66-67	0.3182954221428034
1105	68-69	0.2416763219136513
1105	70-71	0.3095309551343277
1105	72-73	0.26889336227938543
1105	74-75	0.335684850413827
1105	76-77	0.24107685814596636
1105	78-79	0.24427814064365094
1105	80-81	0.22178261088988904
1105	82-83	0.2554263414673983
1105	84-85	0.2393105929834647
1105	86-87	0.3303141847147657
1105	88-89	0.3229406121944578
1105	90-91	0.2812382007695504
1105	92-93	0.28399039562055606
1105	94-95	0.2197822626837933
1105	96-97	0.26290619249319747
1105	98-99	0.2799089521550684
1105	100	0.5122848548024947
1106	1	0.10577074770264971
1106	2	0.20309744531615337
1106	3	0.15481821875359714
1106	4	0.1526247344034175
1106	5	0.08605795119259341
1106	6	0.11375877457794559
1106	7	0.1438419615803781
1106	8	0.10621464177844331
1106	9	0.11091921741369504
1106	10-11	0.19131684845812913
1106	12-13	0.15521154661363568
1106	14-15	0.21788090712860964
1106	16-17	0.17530340335429884
1106	18-19	0.20438087419705653
1106	20-21	0.1724096842325622
1106	22-23	0.2348875440932119
1106	24-25	0.129227701055882
1106	26-27	0.24523149161520763
1106	28-29	0.23619783161336017
1106	30-31	0.25936566556098484
1106	32-33	0.3116113137652263
1106	34-35	0.3578103375719266
1106	36-37	0.17762557341895757
1106	38-39	0.24460584442697098
1106	40-41	0.22337662171579353
1106	42-43	0.3016925404101656
1106	44-45	0.28500646139858077
1106	46-47	0.337583770730447
1106	48-49	0.329585269834908
1106	50-51	0.33360808047341095
1106	52-53	0.3416017154412998
1106	54-55	0.2988845455997833
1106	56-57	0.2744520003159323
1106	58-59	0.2533315864328358
1106	60-61	0.2618477867516731
1106	62-63	0.27557684116752057
1106	64-65	0.26093827294437233
1106	66-67	0.26819312764099124
1106	68-69	0.23229316124675847
1106	70-71	0.2613358305440201
1106	72-73	0.21980650433214066
1106	74-75	0.22402950167260371
1106	76-77	0.22838909666749174
1106	78-79	0.2544959098732704
1106	80-81	0.221222691153244
1106	82-83	0.20065096063308374
1106	84-85	0.22606182329310087
1106	86-87	0.19642856306577983
1106	88-89	0.20071268152420174
1106	90-91	0.2016501092371712
1106	92-93	0.19233832964008712
1106	94-95	0.21824238262328777
1106	96-97	0.1946753929246725
1106	98-99	0.21395702672094075
1106	100	0.45870280567847743
1107	1	0.09179697037671275
1107	2	0.1375103666639106
1107	3	0.11782364784600219
1107	4	0.12112359744319434
1107	5	0.017917539650333936
1107	6	0.04134460274368479
1107	7	0.12847144155012558
1107	8	0.09568074075379229
1107	9	0.043401453096649334
1107	10-11	0.09510093804424713
1107	12-13	0.04523517246136777
1107	14-15	0.12708224723797912
1107	16-17	0.056713210068068065
1107	18-19	0.05448064153307541
1107	20-21	0.13361181273552347
1107	22-23	0.10673411447122305
1107	24-25	0.08990315547553251
1107	26-27	0.1400550925410755
1107	28-29	0.12004078512590155
1107	30-31	0.12207129340924894
1107	32-33	0.19562916964216015
1107	34-35	0.1773408919176589
1107	36-37	0.036348315878449
1107	38-39	0.19186424961535664
1107	40-41	0.17529447701361534
1107	42-43	0.10979027925019125
1107	44-45	0.09960034983050292
1107	46-47	0.19113358879884146
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2216	82-83	-0.9373156226994865
2216	84-85	-0.8653220292405948
2216	86-87	-0.9145875530169292
2216	88-89	-0.7813045025146508
2216	90-91	-0.8190806037568592
2216	92-93	-0.8388077169797796
2216	94-95	-0.8277222219053968
2216	96-97	-0.8493012192548974
2216	98-99	-0.8346232895601311
2216	100	-0.26201804757429414
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	5.0
13	44.0
14	195.0
15	586.0
16	1437.0
17	2942.0
18	5361.0
19	8255.0
20	11909.0
21	17015.0
22	23179.0
23	27478.0
24	28681.0
25	30017.0
26	34563.0
27	42937.0
28	55567.0
29	70048.0
30	87158.0
31	109596.0
32	138383.0
33	177963.0
34	249255.0
35	358531.0
36	579362.0
37	1074135.0
38	974159.0
39	342381.0
40	506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.569804647802655	21.525042838029567	12.879757896619054	26.02539461754872
2	14.463078104040925	20.901212050277408	39.04788691514525	25.58782293053642
3	17.151992114388097	26.729852198689386	29.529644275877807	26.588511411044706
4	11.707212116960832	17.228893613270955	38.94836334335166	32.115530926416554
5	12.476268973869418	38.89713713573394	35.46805300114502	13.158540889251617
6	30.135230757134995	38.99280042231532	17.49443464782721	13.377534172722475
7	26.429222230309108	32.23286546208015	23.74770085352584	17.590211454084912
8	26.628339322121313	34.732993504433864	19.48285715055894	19.155810022885884
9	26.28138947069537	14.168852372108152	21.131031805720664	38.41872635147581
10-11	25.687239254101623	25.783720290804403	27.629226183897572	20.8998142711964
12-13	25.893993863158478	23.51443182100254	28.02864608166184	22.562928234177146
14-15	23.088290665188133	24.666633118053078	25.58643051438112	26.65864570237767
16-17	21.880663627794807	27.11251912773056	26.909953877796376	24.09686336667826
18-19	21.311374139616966	27.436124260414847	29.129091656106915	22.123409943861276
20-21	22.819191616954786	26.608807247287075	28.406959933076532	22.165041202681614
22-23	23.143610506841057	26.31302941020547	28.10236514444525	22.440994938508222
24-25	22.675245055094155	26.271864916664644	28.987744325028103	22.065145703213098
26-27	22.65068114039462	26.549851605651114	27.846145655590327	22.95332159836393
28-29	21.92358027986015	27.015981932100715	28.33390840074651	22.726529387292626
30-31	22.791797292331026	26.71199365988177	28.30485177188154	22.191357275905663
32-33	23.207294730136137	26.80135374463233	27.57187668757576	22.419474837655773
34-35	23.015444603486525	26.613378583950407	28.403073735323424	21.96810307723964
36-37	22.710613947361562	26.151572494885045	28.05156377901956	23.086249778733826
38-39	22.82304411935165	26.335066173186945	28.711487330995322	22.130402376466087
40-41	22.38872220919335	25.596104547255717	29.173035088996173	22.84213815455476
42-43	22.646862333354	25.723136040283475	28.31881288115064	23.31118874521189
44-45	22.77821806376599	26.65647494458237	28.098692350615007	22.46661464103663
46-47	22.790101292733574	26.526388405922113	27.41284583790467	23.270664463439637
48-49	23.680175359619305	26.361034061063176	27.381059885184193	22.577730694133326
50-51	22.93559784098128	26.16658939198302	27.362426353183047	23.535386413852656
52-53	22.36969556470272	26.800039625738943	27.08760702768321	23.742657781875135
54-55	22.451968392857303	26.73301956217602	27.00522188167449	23.80979016329219
56-57	23.601249603517974	26.180820064102043	26.739691242711693	23.478239089668286
58-59	22.373825168889606	26.641747819151572	27.71206993900352	23.272357072955298
60-61	22.539969431572818	27.21105558116403	26.889287392635364	23.359687594627793
62-63	23.07481582118279	26.433130891633038	26.438859102193973	24.0531941849902
64-65	22.138771257710204	26.7385269213131	26.96640874354551	24.156293077431183
66-67	22.801001831569508	26.317599566811968	26.91625446899254	23.965144132625984
68-69	22.344345425024237	26.196567027251906	27.329416135852487	24.129671411871367
70-71	22.285487402655093	26.03441546443917	27.3190228528523	24.36107428005344
72-73	22.829143844067232	25.902721392458705	27.790105953170258	23.478028810303805
74-75	22.89755783179609	26.330967799833704	27.24410349133246	23.527370877037747
76-77	22.66187967488441	26.33815907481444	26.81182640808824	24.18813484221291
78-79	22.422854447227433	26.337683780171396	26.939370795098444	24.300090977502723
80-81	22.062944273272034	26.89152251793267	27.452213245779323	23.593319963015976
82-83	22.87687806683901	25.822840599399953	28.37949821774029	22.92078311602075
84-85	23.156796622916616	26.32405902348162	27.01301674075152	23.506127612850243
86-87	21.776926857714844	26.947524200004853	27.48608831058672	23.789460631693583
88-89	22.74977918309877	25.846079163420683	27.226488054322306	24.177653599158244
90-91	22.37556978847403	25.818583752728202	27.441711526417606	24.36413493238016
92-93	22.733403239965746	26.124144474905048	27.11217094238274	24.030281342746466
94-95	21.569127595777925	26.128322699078904	27.77741836064454	24.525131344498625
96-97	21.919121261050954	26.02467578328225	27.32794477196331	24.72825818370349
98-99	21.346210350674312	26.656407553869887	27.268955434971108	24.728426660484693
100	22.119710843286835	26.022620684446647	26.38212229123496	25.47554618103156
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	137.0
1	92.5
2	76.5
3	94.0
4	64.5
5	54.0
6	108.0
7	226.5
8	359.0
9	416.0
10	353.0
11	279.0
12	290.0
13	367.5
14	489.0
15	660.5
16	842.0
17	1124.5
18	1587.5
19	2127.5
20	2821.5
21	3640.5
22	4411.0
23	5504.5
24	6820.0
25	8169.5
26	10143.5
27	12368.0
28	16114.5
29	23935.5
30	35207.0
31	45923.5
32	61055.5
33	76478.0
34	86143.5
35	97931.0
36	110463.5
37	130023.0
38	139753.0
39	133116.5
40	125812.5
41	117981.0
42	117621.5
43	124682.5
44	137061.0
45	163835.0
46	187530.0
47	204192.0
48	217835.5
49	213733.5
50	202234.0
51	204791.0
52	206175.0
53	196963.0
54	189145.0
55	163227.0
56	140048.0
57	116440.0
58	90960.0
59	87650.5
60	72296.0
61	47860.0
62	34319.5
63	28174.5
64	20282.5
65	9391.0
66	4834.0
67	3025.5
68	1853.5
69	1044.5
70	526.0
71	254.0
72	100.5
73	37.0
74	13.5
75	6.5
76	3.0
77	3.5
78	2.5
79	1.5
80	1.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0060427005525139875
3	0.0
4	0.08545142342662904
5	0.004515177736265099
6	4.492714165437909E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	3.032582061670589E-4
14-15	0.00898542833087582
16-17	0.0
18-19	2.2463570827189544E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	3.369535624078432E-5
60-61	0.0
62-63	0.0
64-65	5.615892706797386E-5
66-67	1.235496395495425E-4
68-69	0.0
70-71	4.3803963113019616E-4
72-73	3.48185347821438E-4
74-75	3.032582061670589E-4
76-77	1.0108606872235296E-4
78-79	4.492714165437909E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06098859479581962
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4451652.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.0838232128569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.14133668569582	49.736843229881195
2	16.124571284419112	21.63395778854161
3	5.230037892092854	10.52552812049099
4	2.1363642334222215	5.732619222126674
5	0.9782566527885271	3.281259817623334
6	0.5144369721658428	2.0706239336958467
7	0.3015021875334383	1.4158143612747962
8	0.17425701087818796	0.9351861209082595
9	0.11030309710231352	0.6659598119257969
>10	0.281827690675083	2.6425422961562246
>50	0.004028621190764936	0.18801547481305225
>100	0.002393778339419967	0.32586984444735945
>500	4.559291318340364E-4	0.21226315654022937
>1k	1.3677873877918965E-4	0.26493133338868496
>5k	9.118582585279311E-5	0.36858548818598963
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6506	0.1461479918016952	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5545	0.12456050023676604	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.2463570827189548E-5	0.0
9	0.0	0.0	0.0	4.4927141654379096E-5	0.0
10-11	0.0	0.0	0.0	4.4927141654379096E-5	0.0
12-13	0.0	0.0	0.0	4.4927141654379096E-5	0.0
14-15	0.0	0.0	0.0	4.4927141654379096E-5	0.0
16-17	0.0	0.0	0.0	4.4927141654379096E-5	0.0
18-19	0.0	0.0	0.0	1.1231785413594774E-4	0.0
20-21	0.0	0.0	0.0	1.4601321037673205E-4	0.0
22-23	0.0	0.0	0.0	1.6847678120392162E-4	0.0
24-25	0.0	0.0	0.0	3.3695356240784325E-4	0.0
26-27	0.0	0.0	0.0	8.42383906019608E-4	0.0
28-29	0.0	0.0	0.0	0.0035492441906959486	0.0
30-31	0.0	0.0	0.0	0.011939387894651245	0.0
32-33	0.0	0.0	0.0	0.02703490749052262	0.0
34-35	0.0	0.0	0.0	0.053092649650062496	0.0
36-37	0.0	0.0	0.0	0.09708755311511322	0.0
38-39	0.0	0.0	0.0	0.1659383977004492	0.0
40-41	0.0	0.0	0.0	0.24164063138807795	0.0
42-43	0.0	0.0	0.0	0.3224982545805467	0.0
44-45	0.0	0.0	0.0	0.41810321202106543	0.0
46-47	0.0	0.0	0.0	0.5399119248315007	0.0
48-49	0.0	0.0	0.0	0.6816682885364804	0.0
50-51	0.0	0.0	0.0	0.8360267154755133	0.0
52-53	0.0	0.0	0.0	0.9883184938984448	0.0
54-55	0.0	0.0	0.0	1.1542456598134805	0.0
56-57	0.0	0.0	0.0	1.3603264585821173	0.0
58-59	0.0	0.0	0.0	1.6095148497681309	0.0
60-61	0.0	0.0	0.0	1.8633756636862002	0.0
62-63	0.0	0.0	0.0	2.130961719379682	0.0
64-65	0.0	0.0	0.0	2.4179563002678552	0.0
66-67	0.0	0.0	0.0	2.7398592702214817	0.0
68-69	0.0	0.0	0.0	3.1014666016121657	0.0
70-71	0.0	0.0	0.0	3.5011384537695216	0.0
72-73	0.0	0.0	0.0	3.916366328724707	0.0
74-75	0.0	0.0	0.0	4.323316377830073	0.0
76-77	0.0	0.0	0.0	4.791097776735468	0.0
78-79	0.0	0.0	0.0	5.297516517463629	0.0
80-81	0.0	0.0	0.0	5.841572971112747	1.1231785413594774E-5
82-83	0.0	0.0	0.0	6.416123722159774	2.2463570827189548E-5
84-85	0.0	0.0	2.2463570827189548E-5	6.973332596528211	2.2463570827189548E-5
86-87	0.0	0.0	2.2463570827189548E-5	7.564102045712469	2.2463570827189548E-5
88	0.0	0.0	2.2463570827189548E-5	8.048854672377805	2.2463570827189548E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4375	0.0	40.743263	94
GGTATCA	7470	0.0	39.929165	1
GTATCAA	12520	0.0	37.08809	1
CAACGCA	13675	0.0	32.27052	5
CTGTGCG	6135	0.0	31.644684	9
ATCAACG	13935	0.0	31.632584	3
TAACGCC	2285	0.0	30.470358	4
CTAACGC	2200	0.0	30.150776	3
TAGTACT	6445	0.0	30.072971	4
ACTGTGC	7060	0.0	29.292053	8
GTACTAG	4775	0.0	29.064993	1
TCTAACG	2335	0.0	28.811823	2
TATCAAC	16065	0.0	28.75758	2
GTGGTAT	2090	0.0	28.587687	1
CGCCTAC	2335	0.0	28.580778	7
AACGCAG	16375	0.0	28.21239	6
TACTGTG	7730	0.0	27.967325	7
GTACTGT	7140	0.0	27.710962	6
CTAGTAC	7885	0.0	26.60941	3
ACGCAGA	16935	0.0	26.030962	7
>>END_MODULE