FastQCFastQC Report
Mon 16 Jan 2017
ERR1146648_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146648_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5650559
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC96990.17164673442043524No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC66850.043.28500794
GTACTAG69700.036.7023121
TACCCCG3450.034.054265
TAGGCAT86750.032.7203525
GCATCGT73250.032.5905158
CTGTGCG95200.031.8383279
CAACGCA115050.031.8200865
TATTCTC31500.031.7774875
GGTATCA90250.031.2109431
GTATCAA134800.030.7684781
ACTGTGC101400.030.5872978
TAGTACT99900.030.4855924
CTAACGC37500.030.4551053
TCTAACG37600.030.3746452
CTAGGCA98200.029.9125614
TAACGCC38650.029.4273364
CGCCTAC38200.029.4015127
ATCAACG124950.029.376493
GTACTGT106450.028.8713596
TACTGTG113700.028.8489577
Produced by FastQC (version 0.11.5)