##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146648_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5650559 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47679795928155 31.0 31.0 34.0 28.0 34.0 2 31.58897447137531 33.0 31.0 34.0 28.0 34.0 3 31.719137168552706 33.0 31.0 34.0 28.0 34.0 4 35.114552737171664 37.0 35.0 37.0 32.0 37.0 5 35.29607495470802 37.0 35.0 37.0 32.0 37.0 6 35.33658758363553 37.0 35.0 37.0 32.0 37.0 7 35.23993201380607 37.0 35.0 37.0 32.0 37.0 8 35.25162802476711 37.0 35.0 37.0 32.0 37.0 9 36.74148770059741 39.0 37.0 39.0 32.0 39.0 10-11 36.58072741121719 39.0 37.0 39.0 32.0 39.0 12-13 36.51117898246882 39.0 37.0 39.0 32.0 39.0 14-15 37.71125449712144 40.0 38.0 41.0 32.0 41.0 16-17 37.761573235497586 40.0 38.0 41.0 32.0 41.0 18-19 37.74407425884766 40.0 38.0 41.0 32.0 41.0 20-21 37.811059047432295 40.0 38.0 41.0 32.0 41.0 22-23 37.76823576216087 40.0 37.5 41.0 31.5 41.0 24-25 37.74112225002872 40.0 37.0 41.0 31.5 41.0 26-27 37.779528538680864 40.0 37.0 41.0 32.0 41.0 28-29 37.78938083824981 40.0 37.0 41.0 32.0 41.0 30-31 37.67178530478135 40.0 37.0 41.0 31.0 41.0 32-33 37.670630463286905 40.0 37.0 41.0 31.0 41.0 34-35 37.61573536352775 40.0 37.0 41.0 31.0 41.0 36-37 37.5092027178196 40.0 36.5 41.0 31.0 41.0 38-39 37.39742758194366 40.0 36.0 41.0 31.0 41.0 40-41 37.298539578119616 40.0 36.0 41.0 30.0 41.0 42-43 37.141027639920225 40.0 36.0 41.0 30.0 41.0 44-45 36.97075855680827 39.5 35.0 41.0 30.0 41.0 46-47 36.95161770720384 39.0 35.0 41.0 30.0 41.0 48-49 36.80057760656955 39.0 35.0 41.0 30.0 41.0 50-51 36.60513260723408 39.0 35.0 41.0 30.0 41.0 52-53 36.37126963898616 39.0 35.0 41.0 29.0 41.0 54-55 36.15371753484921 38.0 35.0 40.0 29.0 41.0 56-57 35.898786827993476 38.0 34.5 40.0 29.0 41.0 58-59 35.57814288816381 37.0 34.0 40.0 28.0 41.0 60-61 35.327926493644256 37.0 34.0 40.0 28.0 41.0 62-63 35.01060682668741 36.0 34.0 39.5 28.0 41.0 64-65 34.84211845235135 35.5 34.0 39.0 28.0 41.0 66-67 34.592209461046245 35.0 33.5 39.0 28.0 41.0 68-69 34.460745742146926 35.0 33.5 39.0 28.0 41.0 70-71 34.39622814309168 35.0 34.0 38.0 28.0 41.0 72-73 34.1868093935485 35.0 34.0 37.0 28.0 40.5 74-75 33.94318694840634 35.0 33.0 37.0 28.0 40.0 76-77 33.693893294451044 35.0 33.0 36.5 27.0 39.0 78-79 33.453213832472144 35.0 33.0 36.0 27.0 39.0 80-81 33.289625769768975 35.0 33.0 36.0 27.0 38.5 82-83 33.0333982885587 35.0 33.0 35.5 26.5 37.0 84-85 32.870547940478104 35.0 33.0 35.0 26.0 37.0 86-87 32.71194389794001 35.0 33.0 35.0 26.0 36.5 88-89 32.5939589339745 35.0 33.0 35.0 26.0 36.0 90-91 32.450522240365956 35.0 33.0 35.0 26.0 36.0 92-93 32.298204124583066 35.0 33.0 35.0 25.0 36.0 94-95 32.24638349940245 35.0 33.0 35.0 25.0 36.0 96-97 32.13939063374084 35.0 33.0 35.0 25.0 35.0 98-99 32.05977010416137 35.0 32.0 35.0 25.0 35.0 100 31.140715281443835 34.0 31.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3120868928828848 1101 2 0.4511322372415627 1101 3 0.4872630183336746 1101 4 0.5479109856788327 1101 5 0.4557274704556491 1101 6 0.4747255499956182 1101 7 0.45338874338920476 1101 8 0.4869298859616933 1101 9 0.6623043511608913 1101 10-11 0.6591739780960069 1101 12-13 0.6020659804254294 1101 14-15 0.7368883836412081 1101 16-17 0.7197407454907747 1101 18-19 0.7037056268357418 1101 20-21 0.7027215539760832 1101 22-23 0.7162436104079362 1101 24-25 0.7408627277090432 1101 26-27 0.6907622762536718 1101 28-29 0.6904711265436987 1101 30-31 0.7623891006508998 1101 32-33 0.6997823363829028 1101 34-35 0.7340508360931182 1101 36-37 0.7475208168751877 1101 38-39 0.7118731161431668 1101 40-41 0.737918469851401 1101 42-43 0.742589395931482 1101 44-45 0.696866187395905 1101 46-47 0.6956412124010214 1101 48-49 0.6681457802926829 1101 50-51 0.6387134382270432 1101 52-53 0.5782300566639904 1101 54-55 0.5745053891927725 1101 56-57 0.5683649802586856 1101 58-59 0.5801958973583012 1101 60-61 0.5434150126835746 1101 62-63 0.5062497673604227 1101 64-65 0.522677516654177 1101 66-67 0.5451442075916546 1101 68-69 0.5361342639187612 1101 70-71 0.49785390100562665 1101 72-73 0.5208827098027484 1101 74-75 0.4690062913965747 1101 76-77 0.4656238575653404 1101 78-79 0.49083982655955083 1101 80-81 0.4574303050692947 1101 82-83 0.4771947922020843 1101 84-85 0.46326261179126504 1101 86-87 0.4810388056640207 1101 88-89 0.49881570459135105 1101 90-91 0.5147585920538376 1101 92-93 0.4787157819650716 1101 94-95 0.453158416435258 1101 96-97 0.4759132015612053 1101 98-99 0.4805108187929932 1101 100 0.5228644539040808 1102 1 0.3046171659652863 1102 2 0.38698105729874044 1102 3 0.42496199598994977 1102 4 0.4771511363420231 1102 5 0.3647409708467535 1102 6 0.37024184125456117 1102 7 0.3923343699318167 1102 8 0.4508129609016649 1102 9 0.5156399765746968 1102 10-11 0.6142199293508952 1102 12-13 0.5714824568830963 1102 14-15 0.7494609276301745 1102 16-17 0.7192350108215706 1102 18-19 0.7185396062552201 1102 20-21 0.6445270016870879 1102 22-23 0.6772978804512277 1102 24-25 0.7118190001752609 1102 26-27 0.6579533292485493 1102 28-29 0.6429053788490933 1102 30-31 0.674961724189167 1102 32-33 0.6760230449253228 1102 34-35 0.7082300325036002 1102 36-37 0.7125967733302261 1102 38-39 0.7485449293843232 1102 40-41 0.6622308357660813 1102 42-43 0.636908287275169 1102 44-45 0.5814132428348486 1102 46-47 0.5781905940205618 1102 48-49 0.5701465644896757 1102 50-51 0.5196976317017601 1102 52-53 0.485482900372439 1102 54-55 0.49235261651388385 1102 56-57 0.4964567095100989 1102 58-59 0.5230027196016565 1102 60-61 0.5061421328931388 1102 62-63 0.5067779031122583 1102 64-65 0.5067601712397547 1102 66-67 0.560005774708074 1102 68-69 0.4644762154905848 1102 70-71 0.5036188466682887 1102 72-73 0.47629652883652085 1102 74-75 0.48025241446203637 1102 76-77 0.46491788935531275 1102 78-79 0.47410851680659505 1102 80-81 0.5097355681861586 1102 82-83 0.4843368635115084 1102 84-85 0.4749765404201156 1102 86-87 0.4663099585982664 1102 88-89 0.461827953353783 1102 90-91 0.4327794436752228 1102 92-93 0.46832820266536146 1102 94-95 0.5063958223294236 1102 96-97 0.45429391352902115 1102 98-99 0.4665246121641644 1102 100 0.48700417079289693 1103 1 0.25439809965848426 1103 2 0.3083778712674743 1103 3 0.32509244523911107 1103 4 0.4322367756195078 1103 5 0.3376353904464011 1103 6 0.31266047391621044 1103 7 0.31974839944679445 1103 8 0.3362792282474487 1103 9 0.5056573772184052 1103 10-11 0.5276137811015005 1103 12-13 0.4325587409988074 1103 14-15 0.6123134145850457 1103 16-17 0.5504052096687815 1103 18-19 0.6091098373627517 1103 20-21 0.5485203641974294 1103 22-23 0.620943969489204 1103 24-25 0.6131570704008098 1103 26-27 0.5369247587798114 1103 28-29 0.5456786954687658 1103 30-31 0.5321132257223553 1103 32-33 0.5686294140071979 1103 34-35 0.6000558880668123 1103 36-37 0.6022279806309001 1103 38-39 0.6130124246475788 1103 40-41 0.5694593857654127 1103 42-43 0.5339010874943213 1103 44-45 0.5177944622599071 1103 46-47 0.4928097037034078 1103 48-49 0.4938686488814312 1103 50-51 0.4854547820176194 1103 52-53 0.505007611583757 1103 54-55 0.48620445768609954 1103 56-57 0.4928283298860592 1103 58-59 0.540434454413635 1103 60-61 0.5179333353786504 1103 62-63 0.44465310114719614 1103 64-65 0.466868028388312 1103 66-67 0.522771682488866 1103 68-69 0.5054913186367216 1103 70-71 0.5152117857270042 1103 72-73 0.48611692089567526 1103 74-75 0.4715422119606387 1103 76-77 0.4066864412797244 1103 78-79 0.4279105529280969 1103 80-81 0.40386802798241916 1103 82-83 0.46362467137704755 1103 84-85 0.422183820678832 1103 86-87 0.37929732050901066 1103 88-89 0.4061150329332577 1103 90-91 0.43698790788253206 1103 92-93 0.36875084736394825 1103 94-95 0.35764327466621637 1103 96-97 0.3772485021552612 1103 98-99 0.36503317782103295 1103 100 0.5286337761112563 1104 1 0.21859346897795007 1104 2 0.3108478262506331 1104 3 0.2780862162429081 1104 4 0.43147578023408784 1104 5 0.27405225180859105 1104 6 0.25637973907269185 1104 7 0.2488342861157804 1104 8 0.33517802152597653 1104 9 0.4013755101584735 1104 10-11 0.4708117006708221 1104 12-13 0.4029530846955467 1104 14-15 0.5490788146738694 1104 16-17 0.4938866309671255 1104 18-19 0.513588964021217 1104 20-21 0.4822957139821753 1104 22-23 0.544772154753538 1104 24-25 0.5706086965580539 1104 26-27 0.5252188941312497 1104 28-29 0.5238931766666965 1104 30-31 0.42986576564980794 1104 32-33 0.4803210873621495 1104 34-35 0.49413450520936664 1104 36-37 0.4670181540815648 1104 38-39 0.48522163166683185 1104 40-41 0.4663131926432058 1104 42-43 0.46934666949410797 1104 44-45 0.4431557440550833 1104 46-47 0.38171088344692095 1104 48-49 0.43366450911408094 1104 50-51 0.3542247458560581 1104 52-53 0.3859735135664977 1104 54-55 0.46433131991715015 1104 56-57 0.4356870491370941 1104 58-59 0.38612172507178144 1104 60-61 0.4398007018568961 1104 62-63 0.4858221766421451 1104 64-65 0.43264902085613954 1104 66-67 0.39132835245011677 1104 68-69 0.40503568303000037 1104 70-71 0.35854034422667524 1104 72-73 0.2976729496246975 1104 74-75 0.3526766066135991 1104 76-77 0.30150557105088893 1104 78-79 0.3196984585038578 1104 80-81 0.32901926504909085 1104 82-83 0.3424426776991041 1104 84-85 0.39283067185012044 1104 86-87 0.3694651187412319 1104 88-89 0.31914779433873264 1104 90-91 0.37428451676997554 1104 92-93 0.38452910699673026 1104 94-95 0.4139989174365013 1104 96-97 0.4019939722593051 1104 98-99 0.34254602812691815 1104 100 0.49297283343613074 1105 1 0.1002258911152687 1105 2 0.20673355424315076 1105 3 0.1760179808811344 1105 4 0.2027934108723386 1105 5 0.15697770420459278 1105 6 0.14358047248123285 1105 7 0.16759610320846718 1105 8 0.22392329229969477 1105 9 0.33013902468344014 1105 10-11 0.34945469382286376 1105 12-13 0.27450420465468284 1105 14-15 0.35943281460740906 1105 16-17 0.30206095455791626 1105 18-19 0.39658606422809584 1105 20-21 0.30106716717803295 1105 22-23 0.3134869756677787 1105 24-25 0.316675786744149 1105 26-27 0.3670390325419959 1105 28-29 0.3839762295121716 1105 30-31 0.4164021006467351 1105 32-33 0.4160750807913871 1105 34-35 0.4086169141604117 1105 36-37 0.3560582845152709 1105 38-39 0.3515786782286838 1105 40-41 0.34551909647379375 1105 42-43 0.38173620631709326 1105 44-45 0.349975567940362 1105 46-47 0.37677245844349017 1105 48-49 0.3339874530735756 1105 50-51 0.3457033144249664 1105 52-53 0.3429549956124731 1105 54-55 0.3492766728341934 1105 56-57 0.33054822263692074 1105 58-59 0.3298043370350925 1105 60-61 0.35617321559609394 1105 62-63 0.3540953083761025 1105 64-65 0.2947949657408273 1105 66-67 0.2703548004951273 1105 68-69 0.2586948629523107 1105 70-71 0.3023854928699379 1105 72-73 0.32108226663778083 1105 74-75 0.2892331744539547 1105 76-77 0.2608189376780956 1105 78-79 0.28091668989211627 1105 80-81 0.26560166461179335 1105 82-83 0.2934558284314477 1105 84-85 0.21153429288276016 1105 86-87 0.2636058427438783 1105 88-89 0.2576580374979969 1105 90-91 0.3013800906455799 1105 92-93 0.2989044512810679 1105 94-95 0.30001043593529175 1105 96-97 0.2239067441183593 1105 98-99 0.23366247261834872 1105 100 0.35660225116504307 1106 1 0.051677646444833414 1106 2 0.17842935336823018 1106 3 0.16562061298809638 1106 4 0.2782509886908926 1106 5 0.13643721431843403 1106 6 0.2125980049610945 1106 7 0.18346955088020422 1106 8 0.16165742004225336 1106 9 0.2972623385867905 1106 10-11 0.3263376845830379 1106 12-13 0.2509790794995794 1106 14-15 0.3589307368133774 1106 16-17 0.2586082407510659 1106 18-19 0.2866628262559914 1106 20-21 0.2541440133682684 1106 22-23 0.24705625801273357 1106 24-25 0.33399530839103875 1106 26-27 0.3626602569077235 1106 28-29 0.3393231179893519 1106 30-31 0.34871589756201615 1106 32-33 0.31901722095759766 1106 34-35 0.2866649290639671 1106 36-37 0.2648253007404051 1106 38-39 0.29135284994512745 1106 40-41 0.3444456630107311 1106 42-43 0.2767872348247451 1106 44-45 0.2781794286375714 1106 46-47 0.25385963544142953 1106 48-49 0.1386740599893841 1106 50-51 0.18595951785300713 1106 52-53 0.100481507409512 1106 54-55 0.16994283623724016 1106 56-57 0.16582824199584678 1106 58-59 0.19348999774619813 1106 60-61 0.16835172512714536 1106 62-63 0.20014490633563753 1106 64-65 0.12128989989292194 1106 66-67 0.1581857149239312 1106 68-69 0.1309587472647067 1106 70-71 0.16466204940631712 1106 72-73 0.1591860090686552 1106 74-75 0.15777903152726225 1106 76-77 0.1343098014624431 1106 78-79 0.1493108788716384 1106 80-81 0.16144783146702935 1106 82-83 0.20416519075865835 1106 84-85 0.10990068792531815 1106 86-87 0.13243286470432736 1106 88-89 0.19423649364335915 1106 90-91 0.1647198116233639 1106 92-93 0.1697166939967829 1106 94-95 0.16933546246546882 1106 96-97 0.16780804423337514 1106 98-99 0.1529094585513917 1106 100 0.14672393371560588 1107 1 0.177140873162422 1107 2 0.16469974473666582 1107 3 0.17318927320228283 1107 4 0.23193935023438428 1107 5 0.158306992351676 1107 6 0.19866069507389028 1107 7 0.16191579626063657 1107 8 0.2052083780768612 1107 9 0.20473018563865253 1107 10-11 0.37036322014271406 1107 12-13 0.18449564061872792 1107 14-15 0.3671025773491934 1107 16-17 0.2691587633803394 1107 18-19 0.20918828672570555 1107 20-21 0.191647247951515 1107 22-23 0.2767445198596121 1107 24-25 0.338214395591379 1107 26-27 0.2853199955777441 1107 28-29 0.3101343313594498 1107 30-31 0.42541784133884164 1107 32-33 0.38720369107026187 1107 34-35 0.3486562792724115 1107 36-37 0.4096481762846551 1107 38-39 0.39626443429877867 1107 40-41 0.3641698870732384 1107 42-43 0.3347128792569194 1107 44-45 0.35102955924329393 1107 46-47 0.3577190475680325 1107 48-49 0.3276296026071961 1107 50-51 0.3216780403705144 1107 52-53 0.33569264883295347 1107 54-55 0.29734949846800873 1107 56-57 0.3095355633566257 1107 58-59 0.354609867882381 1107 60-61 0.3520477163182889 1107 62-63 0.4484806901028051 1107 64-65 0.41597240643061895 1107 66-67 0.3706278244828809 1107 68-69 0.34015227689167915 1107 70-71 0.3522421466835297 1107 72-73 0.33036246972708483 1107 74-75 0.3484269954146555 1107 76-77 0.32971051248336636 1107 78-79 0.3351564854529627 1107 80-81 0.24581199362874884 1107 82-83 0.269912255926684 1107 84-85 0.29748747926848296 1107 86-87 0.27322179821209147 1107 88-89 0.2671740307583832 1107 90-91 0.24639232426456914 1107 92-93 0.23990029767336551 1107 94-95 0.21382425120148696 1107 96-97 0.32097299596677686 1107 98-99 0.29036838529735576 1107 100 0.2923143381610167 1108 1 0.008217109350692198 1108 2 0.06494643300363023 1108 3 0.019983263709942634 1108 4 0.0933432088087045 1108 5 0.054375657715894476 1108 6 0.0766111323057288 1108 7 0.08373997626669194 1108 8 0.12000940610096933 1108 9 0.0953530299637535 1108 10-11 0.24736475842696848 1108 12-13 0.1268077300857655 1108 14-15 0.17586362874235562 1108 16-17 0.2111400547351323 1108 18-19 0.17470016822958456 1108 20-21 0.1319631054109962 1108 22-23 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-0.8555781773914077 2215 42-43 -0.8441238470593646 2215 44-45 -0.7455811508381558 2215 46-47 -0.9169999929316859 2215 48-49 -1.0192827862541094 2215 50-51 -0.9366862580823607 2215 52-53 -0.8797724410647803 2215 54-55 -0.7974921582987164 2215 56-57 -0.8520903249183576 2215 58-59 -0.9010837484809642 2215 60-61 -0.9047305857701176 2215 62-63 -0.9046676579843833 2215 64-65 -0.8510114106457536 2215 66-67 -0.798377087399551 2215 68-69 -0.8160798607181761 2215 70-71 -0.9671796428131216 2215 72-73 -0.8067104871858177 2215 74-75 -0.937284553226057 2215 76-77 -0.909788658822599 2215 78-79 -0.8050987768942903 2215 80-81 -0.7559601457823675 2215 82-83 -0.7384939105670796 2215 84-85 -0.7873670938402881 2215 86-87 -0.7164496029893357 2215 88-89 -0.7172361547587016 2215 90-91 -0.8039859617144174 2215 92-93 -0.8240365598688797 2215 94-95 -0.813326208907192 2215 96-97 -1.0565785692318705 2215 98-99 -0.9263055285523087 2215 100 -0.47934402767791084 2216 1 -0.47687081987081825 2216 2 -0.6850354372599767 2216 3 -0.6367102349665856 2216 4 -0.517602075646856 2216 5 -0.47881082475264947 2216 6 -0.3877269510185428 2216 7 -0.5276317195888467 2216 8 -0.5567482718423875 2216 9 -0.6818738455574831 2216 10-11 -1.047715012413164 2216 12-13 -0.6367707172836035 2216 14-15 -0.7487882803188839 2216 16-17 -0.8188184281685196 2216 18-19 -1.0858440864569516 2216 20-21 -1.1108789120660774 2216 22-23 -0.9890460028847272 2216 24-25 -1.02485437226143 2216 26-27 -1.3474930303838093 2216 28-29 -1.282996066775901 2216 30-31 -1.4035626724789338 2216 32-33 -1.5568654178109682 2216 34-35 -1.2108706106097316 2216 36-37 -1.289921774870571 2216 38-39 -1.2235735146862154 2216 40-41 -1.1488707232573034 2216 42-43 -1.1865565922765242 2216 44-45 -1.0753562456389218 2216 46-47 -1.2151515781173643 2216 48-49 -1.2330658673537442 2216 50-51 -1.2815313165077526 2216 52-53 -1.2162330339409948 2216 54-55 -1.2658414732766445 2216 56-57 -1.2761610895389666 2216 58-59 -1.1893275491129955 2216 60-61 -1.2172601185827787 2216 62-63 -1.2554567538610257 2216 64-65 -1.2156123288335081 2216 66-67 -1.2191445960514926 2216 68-69 -1.1672965952015772 2216 70-71 -1.051514951843771 2216 72-73 -0.9996533019177392 2216 74-75 -1.1369781774789018 2216 76-77 -1.144660969205411 2216 78-79 -1.004585227813969 2216 80-81 -1.032623499902627 2216 82-83 -1.002186140210057 2216 84-85 -1.0497056680952639 2216 86-87 -1.1117423423190758 2216 88-89 -1.0731897958108938 2216 90-91 -1.1956083161137343 2216 92-93 -1.14843666536931 2216 94-95 -1.1747378616938704 2216 96-97 -1.0498847897903971 2216 98-99 -1.1324701356641214 2216 100 -0.9477463016435941 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 3.0 2 14.0 3 33.0 4 49.0 5 36.0 6 50.0 7 239.0 8 599.0 9 961.0 10 1238.0 11 2075.0 12 3388.0 13 5172.0 14 7701.0 15 10831.0 16 13837.0 17 16586.0 18 19289.0 19 22301.0 20 25735.0 21 29852.0 22 35165.0 23 41620.0 24 49007.0 25 57018.0 26 63417.0 27 68277.0 28 78003.0 29 95974.0 30 110986.0 31 127698.0 32 158468.0 33 207161.0 34 309151.0 35 455317.0 36 732853.0 37 1340147.0 38 1091086.0 39 468513.0 40 709.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.837655798126114 21.398764045361666 11.88113060966667 23.882449546845557 2 15.717770591705285 19.96356896048531 40.37827822277269 23.94038222503671 3 18.250227074983947 27.518603157882065 28.821461364687025 25.409708402446956 4 11.772485110279769 16.644593072720383 41.0497697449522 30.533152072047642 5 12.686207260886349 39.25222777958985 35.29561052514027 12.765954434383527 6 29.06448237756822 39.41786899787131 18.296913570196295 13.220735054364177 7 26.53860032786427 32.10845253055963 24.295545764107267 17.057401377468825 8 26.43181995962811 34.89409546044521 19.899987009920952 18.77409757000573 9 26.42951710521868 14.678666255377596 21.64771533699423 37.24410130240949 10-11 25.795281100448303 25.95638978160204 27.558035006782987 20.690294111166672 12-13 25.77471730602977 24.105409301226697 27.549466467393852 22.57040692534968 14-15 23.22540757529664 25.15523707334028 24.896784402087512 26.722570949275564 16-17 21.876617381896224 27.598798944204955 26.351243073988567 24.173340599910258 18-19 21.12568474477224 28.73412320370915 28.01612894376974 22.12406310774887 20-21 23.141076084770297 27.70856209285083 26.804384431200567 22.34597739117831 22-23 23.862993317297537 27.311388485604816 26.41678362568442 22.408834571413223 24-25 23.12790921055298 27.386210783313032 27.518659696286008 21.967220309847985 26-27 23.014142122776732 27.617597056750782 26.191261644845614 23.17699917562687 28-29 21.89117246431201 28.21936541531988 26.91270617949355 22.976755940874558 30-31 22.910689996413446 27.823907837300123 26.85315750924049 22.412244657045942 32-33 23.626046399345583 28.000362464468125 25.67166984442522 22.701921291761064 34-35 23.33055091450376 27.697255369326502 27.01872332194084 21.953470394228898 36-37 22.862305098340858 27.237418923900897 26.619039722001038 23.281236255757207 38-39 23.168319425124825 27.39257659971536 27.30306650179936 22.13603747336046 40-41 22.55982208104367 26.261629428837463 28.143062925238393 23.035485564880474 42-43 22.615920431296274 26.642809011853423 27.20567372873781 23.53559682811249 44-45 22.762639026906356 27.923923867933244 27.018454552888905 22.2949825522715 46-47 22.701046121124616 27.563789740646005 26.483940188372955 23.251223949856424 48-49 23.6837926879004 26.967093356859234 26.688664376384224 22.66044957885614 50-51 23.061300999424912 26.706468073823235 26.769004039083473 23.46322688766838 52-53 22.297691579402304 27.61501676663571 26.57949213914869 23.507799514813293 54-55 22.424926956112778 27.45242976188485 26.515614748842577 23.607028533159795 56-57 23.811492304650628 26.68349761607275 26.241607756573742 23.263402322702877 58-59 22.39056879983383 27.268888698707876 27.385012200446102 22.95553030101219 60-61 22.435360418204738 28.112414647715013 26.35479079939671 23.097434134683546 62-63 23.05033873793063 27.17945119092352 25.836614634873307 23.933595436272544 64-65 21.952155201785505 27.55424619929256 26.490357853674425 24.00324074524751 66-67 22.969323472439342 26.937443006751398 26.395715098892136 23.697518421917124 68-69 22.2095973942355 27.07319438707227 26.78300327667381 23.934204942018415 70-71 22.272399521705548 26.716215852671716 26.947947248851168 24.063437376771567 72-73 23.057235816619265 26.82383668481921 27.459420493263725 22.6595070052978 74-75 23.278916585470753 27.150850942005324 26.804208558889826 22.766023913634097 76-77 22.856431096094315 27.173799152915855 26.460388285247532 23.509381465742297 78-79 22.698282944160972 27.089929165683234 26.49650005500122 23.71528783515457 80-81 22.1181601463931 27.566706719813634 27.32639668757112 22.98873644622215 82-83 23.18746751763638 26.293827906236288 28.564197451002237 21.954507125125094 84-85 23.53653780445294 26.987258912668153 26.813876831996463 22.662326450882436 86-87 21.821978420108852 27.93300069194341 27.358313325322165 22.88670756262557 88-89 23.34882806353807 26.37241546289592 27.011918210903787 23.266838262662226 90-91 22.87820089390332 26.39887444361138 27.312397828748058 23.41052683373724 92-93 23.355964100504217 26.724083842421614 27.09324861906126 22.82670343801291 94-95 21.876079067137333 26.85120797998632 27.873962303555576 23.398750649320768 96-97 22.308518853292757 26.713290092196118 27.301279057192886 23.67691199731824 98-99 21.479645173157245 27.782565860124297 27.110002285831204 23.627786680887255 100 22.636254272572607 26.860158461179996 26.01814600028997 24.48544126595743 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1826.0 1 1200.5 2 786.5 3 1004.0 4 907.5 5 719.0 6 637.5 7 607.5 8 637.5 9 687.0 10 571.0 11 467.5 12 456.0 13 470.0 14 521.0 15 830.5 16 1001.5 17 1085.0 18 1437.5 19 1781.0 20 2164.0 21 2572.5 22 3325.0 23 4596.0 24 6011.5 25 7719.5 26 10749.5 27 13647.5 28 18200.0 29 30548.0 30 49411.0 31 66971.5 32 89147.0 33 109313.5 34 122104.5 35 139484.0 36 155673.0 37 184367.5 38 193563.0 39 173068.0 40 155047.5 41 136342.5 42 131105.0 43 141429.5 44 158303.0 45 197041.0 46 233270.0 47 257087.0 48 272836.5 49 265861.5 50 251184.5 51 259594.0 52 266122.5 53 253613.0 54 239184.0 55 203306.0 56 177597.5 57 145210.5 58 110253.0 59 110129.5 60 92285.0 61 60103.0 62 43662.0 63 37226.0 64 27292.5 65 11650.0 66 5407.5 67 3383.0 68 2175.5 69 1315.0 70 769.0 71 424.5 72 232.5 73 140.0 74 89.5 75 79.0 76 62.0 77 39.5 78 40.0 79 40.5 80 32.5 81 28.0 82 23.5 83 19.0 84 20.0 85 17.5 86 13.5 87 13.5 88 12.0 89 9.5 90 9.5 91 7.5 92 5.0 93 6.5 94 8.0 95 8.0 96 5.0 97 3.0 98 5.5 99 6.0 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06349814239617708 2 0.007645261291847408 3 0.007839932296963892 4 0.009202629332779289 5 0.003574867548502723 6 0.002601512522920299 7 8.848682050749315E-4 8 0.0016458548614393725 9 5.663156512479562E-4 10-11 4.5128278458821504E-4 12-13 2.477630974209808E-4 14-15 1.4157891281198906E-4 16-17 5.397696050957083E-4 18-19 0.0016989469537438684 20-21 0.0011591773486481604 22-23 0.0013361509896631467 24-25 0.0013892430819676426 26-27 0.003627959640807219 28-29 0.0041323345176999295 30-31 0.005141084271485352 32-33 0.00619407743552452 34-35 0.0035306241382489765 36-37 0.008246971671298361 38-39 0.006344505030387259 40-41 0.006123287979118526 42-43 0.014157891281198903 44-45 0.01969716624496798 46-47 0.0180867061117316 48-49 0.02093598173207288 50-51 0.017874337742513617 52-53 0.02466127687543834 54-55 0.01848489680401532 56-57 0.015007364758070837 58-59 0.01717529186050442 60-61 0.015679864593927784 62-63 0.010901576286523157 64-65 0.011043155199335146 66-67 0.013573878265849448 68-69 0.005610064420175066 70-71 0.008547826861023837 72-73 0.008459340040516345 74-75 0.011397102481365119 76-77 0.010423747455782693 78-79 0.012113845727475814 80-81 0.010016708081448226 82-83 0.006680754948315732 84-85 0.010786543419863416 86-87 0.009388451655845025 88-89 0.008246971671298361 90-91 0.012936773158195497 92-93 0.008981412281510556 94-95 0.007503682379035419 96-97 0.009521181886606264 98-99 0.009476938476352517 100 0.27409677520401077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5650559.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.131076304504894 #Duplication Level Percentage of deduplicated Percentage of total 1 72.80280860730582 45.961196653700895 2 15.499591369869387 19.570117709197397 3 5.63935098616596 10.680548922465844 4 2.5647927851314636 6.476725160935122 5 1.3289426118854526 4.194878871762428 6 0.7383484693539931 2.7967640134860803 7 0.4389885643772254 1.9399674388152572 8 0.26503089300366817 1.3385348423412518 9 0.18571299037759245 1.0551834869639014 >10 0.525314601996813 4.800627903390509 >50 0.007929424966313419 0.32644111638071305 >100 0.0028508002507870002 0.2995368603884842 >500 1.6894766124715162E-4 0.07055458213258133 >1k 1.2671074069043E-4 0.2303023067924914 >5k 4.223691356347667E-5 0.25862013124700406 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 9699 0.17164673442043524 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.769736410149863E-5 2 0.0 0.0 0.0 0.0 1.769736410149863E-5 3 0.0 0.0 0.0 0.0 1.769736410149863E-5 4 0.0 0.0 0.0 0.0 1.769736410149863E-5 5 0.0 0.0 0.0 0.0 1.769736410149863E-5 6 0.0 0.0 0.0 0.0 3.539472820299726E-5 7 0.0 0.0 0.0 1.769736410149863E-5 3.539472820299726E-5 8 0.0 0.0 0.0 1.769736410149863E-5 3.539472820299726E-5 9 0.0 0.0 0.0 1.769736410149863E-5 3.539472820299726E-5 10-11 0.0 0.0 0.0 1.769736410149863E-5 5.3092092304495894E-5 12-13 0.0 0.0 0.0 1.769736410149863E-5 1.769736410149863E-4 14-15 0.0 0.0 0.0 1.769736410149863E-5 2.2121705126873286E-4 16-17 0.0 0.0 0.0 1.769736410149863E-5 3.008551897254767E-4 18-19 0.0 0.0 0.0 1.769736410149863E-5 3.185525538269753E-4 20-21 0.0 0.0 0.0 2.6546046152247944E-5 4.1588805638521784E-4 22-23 0.0 0.0 0.0 1.238815487104904E-4 4.2473673843596715E-4 24-25 0.0 0.0 0.0 2.300657333194822E-4 4.2473673843596715E-4 26-27 0.0 0.0 0.0 5.751643332987056E-4 4.2473673843596715E-4 28-29 0.0 0.0 0.0 0.0032297689485235 4.2473673843596715E-4 30-31 0.0 0.0 0.0 0.011998812860816072 4.2473673843596715E-4 32-33 0.0 0.0 0.0 0.026068217321507485 4.2473673843596715E-4 34-35 0.0 0.0 0.0 0.04803064617146728 4.601314666389644E-4 36-37 0.0 0.0 0.0 0.08550481465639063 4.601314666389644E-4 38-39 0.0 0.0 0.0 0.14303009666831193 4.955261948419616E-4 40-41 0.0 0.0 0.0 0.20529827225943487 5.132235589434602E-4 42-43 0.0 0.0 0.0 0.27281371630665213 5.220722409942096E-4 44-45 0.0 0.0 0.0 0.35291198623003495 5.486182871464575E-4 46-47 0.0 0.0 0.0 0.45606992157767046 5.751643332987054E-4 48-49 0.0 0.0 0.0 0.5764208461499118 6.105590615017027E-4 50-51 0.0 0.0 0.0 0.7057266369575116 7.078945640599453E-4 52-53 0.0 0.0 0.0 0.8309974287499697 7.609866563644412E-4 54-55 0.0 8.848682050749315E-6 0.0 0.9690103226955067 7.786840204659398E-4 56-57 0.0 1.769736410149863E-5 0.0 1.1396040639519027 8.052300666181877E-4 58-59 0.0 1.769736410149863E-5 0.0 1.3487515129034136 8.494734768719343E-4 60-61 0.0 1.769736410149863E-5 0.0 1.5665441242185065 8.494734768719343E-4 62-63 0.0 1.769736410149863E-5 0.0 1.798264561081479 9.379602973794275E-4 64-65 0.0 1.769736410149863E-5 0.0 2.0399397652515443 9.645063435316754E-4 66-67 0.0 1.769736410149863E-5 0.0 2.3169566055323023 9.910523896839232E-4 68-69 0.0 1.769736410149863E-5 0.0 2.6258994906521638 0.0010087497537854219 70-71 0.0 1.769736410149863E-5 0.0 2.9734491755594448 0.0010175984358361712 72-73 0.0 1.769736410149863E-5 0.0 3.3317588578404367 0.0010795392101914164 74-75 0.0 1.769736410149863E-5 0.0 3.6833081470346563 0.0011414799845466616 76-77 0.0 1.769736410149863E-5 0.0 4.091258935620352 0.0011503286665974109 78-79 0.0 1.769736410149863E-5 0.0 4.529153664265783 0.0012034207589019068 80-81 0.0 1.769736410149863E-5 0.0 5.005584403242228 0.001212269440952656 82-83 0.0 1.769736410149863E-5 0.0 5.5056853667044265 0.0012476641691556534 84-85 0.0 1.769736410149863E-5 0.0 5.987345676772865 0.001265361533257152 86-87 0.0 1.769736410149863E-5 0.0 6.503409662654615 0.0013184536255616479 88 0.0 1.769736410149863E-5 0.0 6.913528378342744 0.0013626970358153944 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 6685 0.0 43.285007 94 GTACTAG 6970 0.0 36.702312 1 TACCCCG 345 0.0 34.05426 5 TAGGCAT 8675 0.0 32.720352 5 GCATCGT 7325 0.0 32.590515 8 CTGTGCG 9520 0.0 31.838327 9 CAACGCA 11505 0.0 31.820086 5 TATTCTC 3150 0.0 31.777487 5 GGTATCA 9025 0.0 31.210943 1 GTATCAA 13480 0.0 30.768478 1 ACTGTGC 10140 0.0 30.587297 8 TAGTACT 9990 0.0 30.485592 4 CTAACGC 3750 0.0 30.455105 3 TCTAACG 3760 0.0 30.374645 2 CTAGGCA 9820 0.0 29.912561 4 TAACGCC 3865 0.0 29.427336 4 CGCCTAC 3820 0.0 29.401512 7 ATCAACG 12495 0.0 29.37649 3 GTACTGT 10645 0.0 28.871359 6 TACTGTG 11370 0.0 28.848957 7 >>END_MODULE