FastQCFastQC Report
Mon 16 Jan 2017
ERR1146648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5650559
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193080.3417007060717356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158490.2804855236446518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103760.1836278499171498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128250.045.635991
GGGCACC69000.043.42334794
GTATCAA231550.041.1457251
GTGGTAT26900.038.303311
CAGTACG10200.038.2532739
CTGTGCG92800.034.0924159
GTACTAG70350.034.0406721
CAACGCA270700.033.6307035
ATCAACG274500.033.1454543
TAGTACT97250.033.040074
ACTGTGC99200.032.926438
TAGGCAT86400.032.0402685
TATCAAC299250.031.790072
TACTGTG112900.031.095277
GTACTGT106250.030.8295846
AACGCAG314250.029.9998766
CTAACGC33750.029.6903743
TACCCCG3550.029.1265415
TCTAACG34550.029.0052132
CTAGGCA99300.028.8995084
Produced by FastQC (version 0.11.5)