##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146648_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5650559 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19486886164714 33.0 31.0 34.0 30.0 34.0 2 32.276899152809484 34.0 31.0 34.0 30.0 34.0 3 32.43065155146597 34.0 31.0 34.0 30.0 34.0 4 36.01339708159848 37.0 35.0 37.0 35.0 37.0 5 35.927941642587925 37.0 35.0 37.0 35.0 37.0 6 35.969129956876834 37.0 35.0 37.0 35.0 37.0 7 35.89213350395952 37.0 35.0 37.0 35.0 37.0 8 35.9071810417341 37.0 35.0 37.0 35.0 37.0 9 37.630147211983804 39.0 37.0 39.0 35.0 39.0 10-11 37.56705548955422 39.0 37.0 39.0 35.0 39.0 12-13 37.521890754525344 39.0 37.0 39.0 35.0 39.0 14-15 38.74225912869859 40.0 38.0 41.0 34.5 41.0 16-17 38.75336679079008 40.0 38.0 41.0 34.5 41.0 18-19 38.821469610351826 40.0 38.0 41.0 35.0 41.0 20-21 38.82472273982096 40.0 38.0 41.0 35.0 41.0 22-23 38.781561080947924 40.0 38.0 41.0 34.5 41.0 24-25 38.69463684920377 40.0 38.0 41.0 34.0 41.0 26-27 38.549093903806686 40.0 38.0 41.0 34.0 41.0 28-29 38.49469795820201 40.0 38.0 41.0 34.0 41.0 30-31 38.346457049647654 40.0 37.5 41.0 34.0 41.0 32-33 38.17994989522276 40.0 37.0 41.0 33.5 41.0 34-35 38.070807419230555 40.0 37.0 41.0 33.0 41.0 36-37 38.05259223025545 40.0 37.0 41.0 33.0 41.0 38-39 37.946171520375245 40.0 37.0 41.0 33.0 41.0 40-41 37.813035577541974 40.0 37.0 41.0 33.0 41.0 42-43 37.66372486332768 40.0 36.5 41.0 33.0 41.0 44-45 37.42155351355503 40.0 36.0 41.0 32.0 41.0 46-47 37.13376065978605 40.0 35.5 41.0 31.0 41.0 48-49 36.80741011641503 39.0 35.0 41.0 30.5 41.0 50-51 36.56860409739993 39.0 35.0 41.0 30.0 41.0 52-53 36.36699254003011 39.0 35.0 41.0 30.0 41.0 54-55 36.170272622584775 38.5 35.0 41.0 30.0 41.0 56-57 35.92571867668313 38.0 35.0 40.0 29.5 41.0 58-59 35.61880585619936 37.0 35.0 40.0 29.0 41.0 60-61 35.428996228514734 37.0 35.0 40.0 29.0 41.0 62-63 35.200658554312945 36.5 34.5 40.0 29.0 41.0 64-65 34.94403155510809 36.0 34.0 39.0 29.0 41.0 66-67 34.697955547406906 35.0 34.0 39.0 29.0 41.0 68-69 34.45877151269458 35.0 34.0 39.0 28.5 41.0 70-71 34.26981348217053 35.0 34.0 38.0 28.5 41.0 72-73 34.048882951226595 35.0 34.0 37.0 28.0 40.0 74-75 33.80600715787588 35.0 34.0 37.0 28.0 39.5 76-77 33.58285029852799 35.0 34.0 36.0 28.0 39.0 78-79 33.374491532607664 35.0 33.0 36.0 27.0 39.0 80-81 33.265323483924334 35.0 33.0 36.0 27.0 38.5 82-83 33.111392872811344 35.0 33.0 35.0 27.0 37.0 84-85 32.98882039812344 35.0 33.0 35.0 27.0 37.0 86-87 32.859045981114434 35.0 33.0 35.0 27.0 36.5 88-89 32.763053620004676 35.0 33.0 35.0 27.0 36.0 90-91 32.69991384923156 35.0 33.0 35.0 27.0 36.0 92-93 32.63058336352209 35.0 33.0 35.0 27.0 36.0 94-95 32.58799660352189 35.0 33.0 35.0 27.0 35.5 96-97 32.50296882131485 35.0 33.0 35.0 27.0 35.0 98-99 32.43763448890632 35.0 33.0 35.0 27.0 35.0 100 31.22161010972543 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3390808725686796 1101 2 0.40190880137150486 1101 3 0.34663967301546705 1101 4 0.22202736176626559 1101 5 0.23560067325455947 1101 6 0.2530572449665911 1101 7 0.27741921144551185 1101 8 0.26978845753803427 1101 9 0.34145777789809273 1101 10-11 0.3393571595623186 1101 12-13 0.3478809313701916 1101 14-15 0.4250459391828798 1101 16-17 0.4558268757752373 1101 18-19 0.44149178619188234 1101 20-21 0.43859063796972464 1101 22-23 0.45929400683954213 1101 24-25 0.4039799776936519 1101 26-27 0.45974423915793494 1101 28-29 0.42686569994562973 1101 30-31 0.5035211380727844 1101 32-33 0.5322019516153418 1101 34-35 0.6015762869962415 1101 36-37 0.4797226859753678 1101 38-39 0.42612630617347946 1101 40-41 0.4397732592374126 1101 42-43 0.4447932461018027 1101 44-45 0.457552800992687 1101 46-47 0.47903074742838214 1101 48-49 0.436362806594488 1101 50-51 0.3964597875760134 1101 52-53 0.4334747490669102 1101 54-55 0.4303895173326353 1101 56-57 0.4335248123320241 1101 58-59 0.44018450629824457 1101 60-61 0.3661373446852991 1101 62-63 0.43780420018584465 1101 64-65 0.39588326020015785 1101 66-67 0.40066675055085454 1101 68-69 0.4132351003837016 1101 70-71 0.36904422143471294 1101 72-73 0.41058051944477114 1101 74-75 0.40608570503268737 1101 76-77 0.3731329102290104 1101 78-79 0.4172754019179976 1101 80-81 0.4040025096071318 1101 82-83 0.38192844465365994 1101 84-85 0.34478094267222303 1101 86-87 0.30000879050659535 1101 88-89 0.3802451199523347 1101 90-91 0.4235230716749214 1101 92-93 0.36544212857390335 1101 94-95 0.36550180036102375 1101 96-97 0.38436703447781184 1101 98-99 0.36377778836130403 1101 100 0.7474526888828095 1102 1 0.29280273369453624 1102 2 0.3521626948920584 1102 3 0.30296643958067193 1102 4 0.20993049076634662 1102 5 0.1817309379365497 1102 6 0.21358558277364637 1102 7 0.24866384216613113 1102 8 0.2793281844977926 1102 9 0.3200513001509364 1102 10-11 0.28466085929680673 1102 12-13 0.32330621186698494 1102 14-15 0.41685016581214995 1102 16-17 0.39847194247374773 1102 18-19 0.37796845704735205 1102 20-21 0.40231648371997863 1102 22-23 0.46014018068928664 1102 24-25 0.4368964355765499 1102 26-27 0.4438841533290159 1102 28-29 0.4679297800084967 1102 30-31 0.44933723870774145 1102 32-33 0.45961448099587443 1102 34-35 0.47518496378831543 1102 36-37 0.4582962811287743 1102 38-39 0.49190930475407413 1102 40-41 0.43072474919976855 1102 42-43 0.42976145144017863 1102 44-45 0.43430761279057606 1102 46-47 0.3679694332946468 1102 48-49 0.3933172409945982 1102 50-51 0.4339817839515945 1102 52-53 0.48076159691876796 1102 54-55 0.4828492890531919 1102 56-57 0.419967145732528 1102 58-59 0.32433497666131217 1102 60-61 0.4047245698156203 1102 62-63 0.4263686702391496 1102 64-65 0.39064820832884095 1102 66-67 0.3765681097381304 1102 68-69 0.4004575658058158 1102 70-71 0.37453163550257074 1102 72-73 0.37826861237044795 1102 74-75 0.3824230659735477 1102 76-77 0.37437329063057234 1102 78-79 0.3649894295061671 1102 80-81 0.3260243409576802 1102 82-83 0.33081539483774947 1102 84-85 0.33219116035013485 1102 86-87 0.3476090785068777 1102 88-89 0.36767197934361917 1102 90-91 0.3399485382375218 1102 92-93 0.3188205356007785 1102 94-95 0.3222671619869715 1102 96-97 0.3426576823201515 1102 98-99 0.3455424400097513 1102 100 0.5438775683715882 1103 1 0.2501156685408006 1103 2 0.36492282661097164 1103 3 0.2427011754829067 1103 4 0.22061384211402668 1103 5 0.23198361491274255 1103 6 0.2577014060296108 1103 7 0.24594821206035533 1103 8 0.1917162686674061 1103 9 0.24160645149652282 1103 10-11 0.2908789731091872 1103 12-13 0.2695601578476712 1103 14-15 0.3976447230245199 1103 16-17 0.3826668010293943 1103 18-19 0.36133856148497046 1103 20-21 0.38450613896195307 1103 22-23 0.4029219847851948 1103 24-25 0.37033453122006676 1103 26-27 0.41530264296829245 1103 28-29 0.43618477449057735 1103 30-31 0.32956977645407903 1103 32-33 0.3132061169618652 1103 34-35 0.43596127144466834 1103 36-37 0.3752586604809238 1103 38-39 0.34201079135513623 1103 40-41 0.3477843376559733 1103 42-43 0.350454969150789 1103 44-45 0.3967979822469303 1103 46-47 0.46788655112951716 1103 48-49 0.42653163877776734 1103 50-51 0.4194788542160879 1103 52-53 0.40220777295584753 1103 54-55 0.38262883533879943 1103 56-57 0.32616608285480453 1103 58-59 0.37138617562437304 1103 60-61 0.3689451559306889 1103 62-63 0.3829785476096177 1103 64-65 0.4208256161788384 1103 66-67 0.3553326714453675 1103 68-69 0.32348648160317595 1103 70-71 0.34104839353366856 1103 72-73 0.36248700333499073 1103 74-75 0.30920116054501534 1103 76-77 0.2866942934372503 1103 78-79 0.31292651086004497 1103 80-81 0.24768029960980442 1103 82-83 0.2527737589417498 1103 84-85 0.2724743956990068 1103 86-87 0.34253103497719195 1103 88-89 0.32225608518577076 1103 90-91 0.26318278212467305 1103 92-93 0.2837347761087443 1103 94-95 0.27675382817662353 1103 96-97 0.32538428488501836 1103 98-99 0.2917508279713985 1103 100 0.5529010038677988 1104 1 0.2512565596388896 1104 2 0.2707229676008751 1104 3 0.2328846715023758 1104 4 0.15042573214906696 1104 5 0.15049975077938882 1104 6 0.1891108739192262 1104 7 0.21278528589958512 1104 8 0.20883935943174947 1104 9 0.23055946943934202 1104 10-11 0.26201700140629924 1104 12-13 0.26830763032256044 1104 14-15 0.32526264277257866 1104 16-17 0.34912475092524176 1104 18-19 0.2890416228205126 1104 20-21 0.3754063539629868 1104 22-23 0.3553097548290083 1104 24-25 0.38751393027141745 1104 26-27 0.393460352817641 1104 28-29 0.3884956300074762 1104 30-31 0.3332829514247777 1104 32-33 0.34389482797302406 1104 34-35 0.4120430299660569 1104 36-37 0.30911979242166154 1104 38-39 0.3722948868834379 1104 40-41 0.4378653797593728 1104 42-43 0.4031545633128246 1104 44-45 0.36339607772717386 1104 46-47 0.3157535915198011 1104 48-49 0.31439901041143514 1104 50-51 0.31749383428877564 1104 52-53 0.25736807167439224 1104 54-55 0.2888275025518894 1104 56-57 0.2717960558177168 1104 58-59 0.2764402395291228 1104 60-61 0.2605122583141153 1104 62-63 0.3332589707509541 1104 64-65 0.25346172027761327 1104 66-67 0.2025571212938786 1104 68-69 0.27319389692144824 1104 70-71 0.25268922272814365 1104 72-73 0.18750449432817362 1104 74-75 0.20382832042432852 1104 76-77 0.19040910610237916 1104 78-79 0.19202838164268599 1104 80-81 0.18000249272620295 1104 82-83 0.15411543508046321 1104 84-85 0.16785634593869503 1104 86-87 0.18662593722342535 1104 88-89 0.2067125144182853 1104 90-91 0.19996087010053998 1104 92-93 0.19359619088749014 1104 94-95 0.19426024285279198 1104 96-97 0.20892093491949026 1104 98-99 0.22965846975456117 1104 100 0.46411353198007177 1105 1 0.11800447293622085 1105 2 0.1115481493350714 1105 3 0.08971856939346168 1105 4 0.12085532636336183 1105 5 0.14589790979098183 1105 6 0.10156713148300867 1105 7 0.169563540462633 1105 8 0.14066922470002652 1105 9 0.13019795416650481 1105 10-11 0.14662142372637277 1105 12-13 0.14019334506296133 1105 14-15 0.23239539932062314 1105 16-17 0.23633573490488402 1105 18-19 0.20259507376518116 1105 20-21 0.22396598094753273 1105 22-23 0.24078362096257422 1105 24-25 0.2066643360375835 1105 26-27 0.21289921871598239 1105 28-29 0.19266522961407873 1105 30-31 0.2207812487185521 1105 32-33 0.2554347920827169 1105 34-35 0.16832267636269904 1105 36-37 0.19668059837762542 1105 38-39 0.22232956963414097 1105 40-41 0.22992366059899183 1105 42-43 0.2239843283548666 1105 44-45 0.24231713177867675 1105 46-47 0.29106775984360667 1105 48-49 0.25428604402089405 1105 50-51 0.2393193686907651 1105 52-53 0.2897355595755684 1105 54-55 0.24032609711223785 1105 56-57 0.13464994846714262 1105 58-59 0.19809634926381392 1105 60-61 0.25410878034631423 1105 62-63 0.24702679879835898 1105 64-65 0.23432628261058142 1105 66-67 0.21749363337650607 1105 68-69 0.2104993369405932 1105 70-71 0.29415477915979693 1105 72-73 0.1859407796169137 1105 74-75 0.18194328740354138 1105 76-77 0.1876069694925917 1105 78-79 0.21272940998875356 1105 80-81 0.15289408581516994 1105 82-83 0.16684093861888982 1105 84-85 0.1556954237520003 1105 86-87 0.15502991806386035 1105 88-89 0.14923565794960325 1105 90-91 0.2093317397415717 1105 92-93 0.14258773502999134 1105 94-95 0.18651786190478958 1105 96-97 0.19621875709362513 1105 98-99 0.24060800521608883 1105 100 0.4047944397576231 1106 1 0.11504185027067138 1106 2 0.16728455046578006 1106 3 0.13000180554160323 1106 4 0.10285713677931341 1106 5 0.07700783749230311 1106 6 0.10052334130653406 1106 7 0.12878501081937088 1106 8 0.0665349157889139 1106 9 0.0860638282859938 1106 10-11 0.14493714547817405 1106 12-13 0.10246800120215482 1106 14-15 0.1507320328472943 1106 16-17 0.12774497026916265 1106 18-19 0.14064121313409572 1106 20-21 0.11367730995439729 1106 22-23 0.15943334393307396 1106 24-25 0.1840521950149423 1106 26-27 0.19442087798039864 1106 28-29 0.17078701616411252 1106 30-31 0.21853192962147006 1106 32-33 0.22290011135499554 1106 34-35 0.2556126137053951 1106 36-37 0.13036405002578277 1106 38-39 0.16364135046209327 1106 40-41 0.19785545001457194 1106 42-43 0.25391565670260974 1106 44-45 0.21483568253816543 1106 46-47 0.16615005748913347 1106 48-49 0.2320015132222082 1106 50-51 0.2712372468815687 1106 52-53 0.22385778873167794 1106 54-55 0.17694525905108804 1106 56-57 0.11538862954547824 1106 58-59 0.0771850862008634 1106 60-61 0.0456037147629047 1106 62-63 0.12855621041369858 1106 64-65 0.11346240384200712 1106 66-67 0.11295459620315285 1106 68-69 0.1771033213580253 1106 70-71 0.18750475381852283 1106 72-73 0.11701603862504584 1106 74-75 0.1184934954738921 1106 76-77 0.14109170677729566 1106 78-79 0.21431560023488316 1106 80-81 0.18683469677660014 1106 82-83 0.15303371993440607 1106 84-85 0.10422541429419141 1106 86-87 0.08083176665174818 1106 88-89 0.08176054177938852 1106 90-91 0.08129234737776869 1106 92-93 0.1256311535379666 1106 94-95 0.13505392922154158 1106 96-97 0.11490304295928411 1106 98-99 0.07798918013817513 1106 100 0.28177485331473306 1107 1 0.16976192348311514 1107 2 0.20902924842158654 1107 3 0.16824476616239536 1107 4 0.12416884667447903 1107 5 0.08763812004962546 1107 6 0.04237341920641313 1107 7 0.13776267930953878 1107 8 0.13800803998728384 1107 9 0.1543671667357458 1107 10-11 0.15200644953979037 1107 12-13 0.0775935136157031 1107 14-15 0.1876844013665746 1107 16-17 0.1816040622447872 1107 18-19 0.17771935336276812 1107 20-21 0.19070415405843733 1107 22-23 0.1275997265561415 1107 24-25 0.11780103757654103 1107 26-27 0.19515801899721197 1107 28-29 0.24233819370058285 1107 30-31 0.1709714377552629 1107 32-33 0.22440736100195835 1107 34-35 0.17656908301476903 1107 36-37 0.10665776955256234 1107 38-39 0.21312399177011088 1107 40-41 0.25623089334429494 1107 42-43 0.2252299569102476 1107 44-45 0.1979029501220424 1107 46-47 0.2788631391333496 1107 48-49 0.2631109014565496 1107 50-51 0.2875751599438061 1107 52-53 0.27393708827731444 1107 54-55 0.3156630538469116 1107 56-57 0.1600891280390755 1107 58-59 0.24600729942945776 1107 60-61 0.2605891857293372 1107 62-63 0.23966571929998537 1107 64-65 0.18327459269361412 1107 66-67 0.2326247570840394 1107 68-69 0.21399935918008595 1107 70-71 0.2207357640763732 1107 72-73 0.17121777152556916 1107 74-75 0.19756028341339515 1107 76-77 0.21002901031440047 1107 78-79 0.21066360360933345 1107 80-81 0.16697558596234785 1107 82-83 0.2170110645693697 1107 84-85 0.2329211472478434 1107 86-87 0.21839623738608793 1107 88-89 0.18301368679973962 1107 90-91 0.1652349156786812 1107 92-93 0.13219255526796303 1107 94-95 0.12226485008476828 1107 96-97 0.13398019161950714 1107 98-99 0.19864471810982565 1107 100 0.2657026655659216 1108 1 0.10298248503726626 1108 2 0.11875308083972413 1108 3 0.11703166555485467 1108 4 0.09041923861536816 1108 5 0.09764291713813833 1108 6 0.07750107803961725 1108 7 0.06641207109767322 1108 8 0.055745448969894085 1108 9 0.032477757045626277 1108 10-11 0.01173876396081397 1108 12-13 0.057024040250261976 1108 14-15 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-0.39542343383125456 2215 4 -0.4877312592660701 2215 5 -0.34307366699203357 2215 6 -0.3178248460268023 2215 7 -0.4086746873100182 2215 8 -0.3609703592411506 2215 9 -0.4441053081391857 2215 10-11 -0.46836807705944494 2215 12-13 -0.4610473062736915 2215 14-15 -0.6245975369920487 2215 16-17 -0.6392315965907542 2215 18-19 -0.5992761718489206 2215 20-21 -0.5358076692654237 2215 22-23 -0.5452355195654448 2215 24-25 -0.5722294617996297 2215 26-27 -0.5784550931473547 2215 28-29 -0.5838779970205863 2215 30-31 -0.5690286327106051 2215 32-33 -0.5415777695684625 2215 34-35 -0.520929216674709 2215 36-37 -0.615560174997178 2215 38-39 -0.7965232226652859 2215 40-41 -0.8222959734649748 2215 42-43 -0.7412787736306896 2215 44-45 -0.709181052958499 2215 46-47 -0.7539999039726837 2215 48-49 -0.7184994231200363 2215 50-51 -0.7617474753335571 2215 52-53 -0.7852937037430863 2215 54-55 -0.7257045379337299 2215 56-57 -0.785284677185146 2215 58-59 -0.7302573275743924 2215 60-61 -0.7492232634497284 2215 62-63 -0.7936556091184315 2215 64-65 -0.7667812579356195 2215 66-67 -0.8202653155971262 2215 68-69 -0.8602686431902455 2215 70-71 -0.7611863848219187 2215 72-73 -0.7398415480302916 2215 74-75 -0.7121613162317502 2215 76-77 -0.7018918573395467 2215 78-79 -0.7827689261199353 2215 80-81 -0.7911528112832755 2215 82-83 -0.7409934644828056 2215 84-85 -0.6549154384967508 2215 86-87 -0.5630485901286448 2215 88-89 -0.6647119398925625 2215 90-91 -0.6491563824142119 2215 92-93 -0.6226568337132363 2215 94-95 -0.6857987545673581 2215 96-97 -0.6629631276427652 2215 98-99 -0.7129134946432956 2215 100 -0.3293153873263748 2216 1 -0.33520816727064684 2216 2 -0.22866125615581723 2216 3 -0.29858420159043675 2216 4 -0.3786300380081542 2216 5 -0.341131512681649 2216 6 -0.37552912610121325 2216 7 -0.35984921466322817 2216 8 -0.4013258734130787 2216 9 -0.47406200146628663 2216 10-11 -0.558638515853545 2216 12-13 -0.48857714180854117 2216 14-15 -0.7302143560984788 2216 16-17 -0.6665167142801067 2216 18-19 -0.6051802290919497 2216 20-21 -0.6136528578265583 2216 22-23 -0.6116996059254802 2216 24-25 -0.6330921087329813 2216 26-27 -0.634911983765825 2216 28-29 -0.6212311332574032 2216 30-31 -0.6731377560618981 2216 32-33 -0.6113398577531797 2216 34-35 -0.700177192089491 2216 36-37 -0.8261724671888189 2216 38-39 -0.8791986397434144 2216 40-41 -0.978447810571204 2216 42-43 -0.9091866013309513 2216 44-45 -0.9262974534731967 2216 46-47 -0.8276788695117574 2216 48-49 -0.8671984831501973 2216 50-51 -0.8831330244194433 2216 52-53 -0.9097766229037276 2216 54-55 -0.8407585651481 2216 56-57 -0.7272096841964668 2216 58-59 -0.7966474731663737 2216 60-61 -0.8011349715615026 2216 62-63 -0.8107137269100733 2216 64-65 -0.7113282219798691 2216 66-67 -0.7652560982279724 2216 68-69 -0.7339817084717666 2216 70-71 -0.7729906369902082 2216 72-73 -0.7647044357495858 2216 74-75 -0.6545357787258581 2216 76-77 -0.7357330680613359 2216 78-79 -0.7163511179817874 2216 80-81 -0.8245836420434642 2216 82-83 -0.7637699003081408 2216 84-85 -0.7760029975884706 2216 86-87 -0.7793476058987352 2216 88-89 -0.7250485461052563 2216 90-91 -0.6876393335868585 2216 92-93 -0.6812891487169175 2216 94-95 -0.7718834847722817 2216 96-97 -0.7385073838891074 2216 98-99 -0.7457628204078759 2216 100 -0.21206279661584304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 16.0 13 80.0 14 334.0 15 1057.0 16 2485.0 17 5075.0 18 9204.0 19 14495.0 20 21393.0 21 31048.0 22 43378.0 23 50901.0 24 51532.0 25 47717.0 26 45560.0 27 52163.0 28 64341.0 29 80922.0 30 101185.0 31 127001.0 32 160786.0 33 208505.0 34 304993.0 35 453371.0 36 730245.0 37 1406453.0 38 1147461.0 39 487834.0 40 1021.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.4577140067027 22.024865150509886 13.118737455887109 24.398683386900306 2 14.487120490755851 20.68454140310014 39.99755754806144 24.83078055808257 3 17.133048252394143 27.77091611644087 29.684408215187208 25.411627415977783 4 11.389775491918538 17.304701989781655 39.8006994332472 31.504823085052596 5 12.090330326758798 39.25949562889228 35.32254307925053 13.327630965098392 6 28.900943553165387 39.73506323979446 17.700311261405073 13.663681945635084 7 25.523271422622585 32.92413473574375 24.310506026220068 17.242087815413594 8 26.838636672938023 34.174901987573264 19.545641413530944 19.44081992595777 9 26.529605300997655 13.8200839952295 21.79876362674914 37.8515470770237 10-11 25.745116545106423 25.92547923134684 27.317491950796374 21.01191227275036 12-13 26.0746845169897 24.019279920404095 28.017108801244202 21.888926761362008 14-15 23.298456215637597 24.654319542221348 26.064262711041696 25.982961531099363 16-17 21.148518226249827 26.957067433505248 27.574351493365523 24.3200628468794 18-19 20.525972833125508 27.048505652008174 30.647229884199046 21.778291630667272 20-21 22.285945514417246 26.254712144409076 29.788185558278396 21.671156782895284 22-23 22.834961992255987 25.821790375076166 29.53306035739119 21.810187275276657 24-25 22.192954714745923 25.934080150300176 30.524378207536635 21.34858692741727 26-27 22.122067922837367 26.180772557193016 29.235665002347556 22.461494517622064 28-29 21.157703158218506 26.751954983568883 29.926870952059787 22.163470906152824 30-31 22.25317884478332 26.316971471318144 29.734845702876477 21.695003981022055 32-33 22.963436006950815 26.544010955376272 28.733635026198296 21.758918011474616 34-35 22.53010719824357 26.36858583372017 29.76360391954141 21.33770304849485 36-37 22.24226841981475 25.832205273849894 29.370766679898395 22.55475962643696 38-39 22.44884975097154 25.853725268597323 30.234893574246367 21.462531406184766 40-41 21.811213722394545 25.081111444018195 30.733888452452224 22.373786381135037 42-43 22.184583861525915 25.325830594813713 29.562172521338155 22.927413022322217 44-45 22.28883018476579 26.56183220102648 29.234886318327092 21.91445129588064 46-47 22.38035210321669 26.33269557932233 28.344815088206314 22.942137229254662 48-49 23.564004906417225 26.1032315563823 28.08527439497579 22.247489142224687 50-51 22.74969609201497 26.025389700381858 27.884878292572466 23.340035915030708 52-53 21.842998188320838 26.889905936740067 27.671430384144298 23.595665490794804 54-55 22.068321028061117 26.87114673079248 27.404058607298854 23.656473633847554 56-57 23.51201005068702 26.00417940950621 27.151260609790995 23.332549930015773 58-59 22.03740268437253 26.58944065160302 28.242304254253163 23.130852409771286 60-61 22.226385035533653 27.401802193375914 27.18269112843526 23.189121642655177 62-63 22.83582031441491 26.417687170419775 26.648903232405857 24.097589282759458 64-65 21.790073876820408 26.764311360946962 27.194746993414544 24.25086776881809 66-67 22.58420290991297 26.22267366327543 27.201578891388746 23.991544535422854 68-69 22.04519057318046 26.01070973685966 27.76312042755416 24.180979262405717 70-71 22.057096206676434 25.704407000189182 27.804943356670954 24.433553436463427 72-73 22.782985050801365 25.66184417516424 28.32314875559615 23.232022018438247 74-75 22.913423980765014 26.259256421115374 27.590660852234045 23.23665874588557 76-77 22.548443054767038 26.263115532783342 27.079310127576917 24.1091312848727 78-79 22.201658862864416 26.289901483172233 27.316897491973137 24.19154216199021 80-81 21.697446217268062 26.841662922199376 28.004087737160166 23.456803123372396 82-83 22.72245099998071 25.47524943992267 29.27795285386809 22.524346706228535 84-85 23.130490275386915 26.22121988284699 27.379308843602907 23.26898099816319 86-87 21.34021607419726 27.043811063648747 28.010918919703343 23.60505394245065 88-89 22.579827942686734 25.665558044788135 27.71193080189057 24.042683210634557 90-91 22.125580849611516 25.52736817720158 28.062418249238704 24.284632723948196 92-93 22.60766589641839 25.974695600913112 27.559875049530497 23.857763453138002 94-95 21.10188567184238 25.985694512702196 28.40786194781791 24.50455786763752 96-97 21.569343847219365 25.809163305789745 27.848899551354123 24.772593295636767 98-99 20.668096731668495 26.816895461139335 27.769110985302515 24.74589682188966 100 21.761597653559676 25.921899962119337 26.57474364144176 25.741758742879224 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1255.0 1 794.5 2 455.5 3 560.0 4 379.0 5 262.5 6 564.0 7 1280.5 8 2045.0 9 2207.0 10 1674.0 11 1213.5 12 1159.0 13 1273.0 14 1586.0 15 2304.0 16 3038.0 17 3864.5 18 5200.0 19 6846.5 20 8688.5 21 10377.0 22 12175.5 23 14042.5 24 15463.5 25 17070.0 26 19836.0 27 21971.0 28 25518.5 29 36575.0 30 53812.0 31 68452.5 32 88897.5 33 108336.0 34 118055.5 35 133522.5 36 149863.0 37 177792.0 38 187366.5 39 168460.0 40 151381.0 41 133022.5 42 127278.5 43 136240.0 44 152232.5 45 192296.0 46 228828.5 47 251407.5 48 269050.0 49 262034.5 50 246775.5 51 254040.0 52 259511.5 53 247824.0 54 238679.0 55 205029.0 56 175445.5 57 144474.0 58 111170.0 59 111802.5 60 93489.5 61 59543.0 62 42118.5 63 35770.0 64 25928.5 65 10393.5 66 4486.0 67 2826.0 68 1768.5 69 1028.0 70 581.5 71 281.0 72 128.5 73 56.0 74 18.0 75 5.0 76 3.0 77 1.5 78 0.5 79 0.5 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.008830984686647817 3 0.0 4 0.09586662133781808 5 0.004866775127912124 6 5.309209230449589E-5 7 3.5394728202997265E-5 8 0.0 9 0.0 10-11 0.0 12-13 3.0085518972547675E-4 14-15 0.010255622496818457 16-17 0.0 18-19 1.7697364101498632E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.5394728202997265E-5 60-61 0.0 62-63 0.0 64-65 7.963813845674384E-5 66-67 2.1236836921798355E-4 68-69 0.0 70-71 4.6013146663896436E-4 72-73 3.8934201023296984E-4 74-75 3.8934201023296984E-4 76-77 1.238815487104904E-4 78-79 2.6546046152247944E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.06863037798561168 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5650559.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.04947201497311 #Duplication Level Percentage of deduplicated Percentage of total 1 71.06833287781049 42.67615866296875 2 16.421731409584094 19.722326014344503 3 6.043010304514452 10.886387345014043 4 2.7334205642569347 6.565618467139954 5 1.3719601215618986 4.119274046269517 6 0.7828654253479496 2.8206393270553036 7 0.4643006626103183 1.951670675216625 8 0.2999518292345207 1.4409559180366676 9 0.20915198141688335 1.1303519449472383 >10 0.5855529357828044 5.11959953771258 >50 0.011375616713923868 0.46544247493157526 >100 0.00675790538082474 0.836526566486852 >500 7.500616393225533E-4 0.32598944868753776 >1k 7.059403331730824E-4 0.732681396718769 >5k 0.0 0.0 >10k+ 1.323638124699525E-4 1.2063781744700817 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19308 0.3417007060717356 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15849 0.2804855236446518 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10376 0.1836278499171498 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.3092092304495894E-5 2 0.0 0.0 0.0 0.0 5.3092092304495894E-5 3 0.0 0.0 0.0 0.0 5.3092092304495894E-5 4 0.0 0.0 0.0 0.0 5.3092092304495894E-5 5 0.0 0.0 0.0 0.0 7.078945640599452E-5 6 0.0 0.0 0.0 0.0 8.848682050749316E-5 7 0.0 0.0 0.0 0.0 8.848682050749316E-5 8 0.0 0.0 0.0 0.0 8.848682050749316E-5 9 0.0 0.0 0.0 0.0 8.848682050749316E-5 10-11 0.0 0.0 0.0 0.0 8.848682050749316E-5 12-13 0.0 0.0 0.0 0.0 2.389144153702315E-4 14-15 0.0 0.0 0.0 0.0 3.008551897254767E-4 16-17 0.0 0.0 0.0 0.0 3.3624991792847395E-4 18-19 0.0 8.848682050749315E-6 0.0 0.0 3.3624991792847395E-4 20-21 0.0 2.6546046152247944E-5 0.0 8.848682050749315E-6 4.424341025374658E-4 22-23 0.0 3.539472820299726E-5 0.0 9.733550255824246E-5 4.424341025374658E-4 24-25 0.0 3.539472820299726E-5 0.0 1.9467100511648495E-4 4.424341025374658E-4 26-27 0.0 3.539472820299726E-5 0.0 5.486182871464576E-4 4.689801486897137E-4 28-29 0.0 3.539472820299726E-5 0.0 0.003212071584422001 4.7782883074046304E-4 30-31 0.0 3.539472820299726E-5 0.0 0.011928023404410078 4.7782883074046304E-4 32-33 0.0 3.539472820299726E-5 0.0 0.026032822593304486 5.043748768927109E-4 34-35 0.0 3.539472820299726E-5 0.0 0.04804834353556878 5.309209230449589E-4 36-37 0.0 3.539472820299726E-5 0.0 0.08541632783588314 5.309209230449589E-4 38-39 0.0 3.539472820299726E-5 0.0 0.14288851775549993 5.309209230449589E-4 40-41 0.0 3.539472820299726E-5 0.0 0.2052717262132826 5.309209230449589E-4 42-43 0.0 3.539472820299726E-5 0.0 0.27285795971690585 5.397696050957082E-4 44-45 0.0 3.539472820299726E-5 0.0 0.35300932173259314 5.574669691972068E-4 46-47 0.0 3.539472820299726E-5 0.0 0.45629998731099 5.663156512479561E-4 48-49 0.0 3.539472820299726E-5 0.0 0.5766420632011806 6.548024717554493E-4 50-51 0.0 3.539472820299726E-5 0.0 0.7062133144703029 7.432892922629425E-4 52-53 0.0 3.539472820299726E-5 0.0 0.8317672640883849 7.963813845674384E-4 54-55 0.0 3.539472820299726E-5 0.0 0.9703376250031192 7.963813845674384E-4 56-57 0.0 3.539472820299726E-5 0.0 1.1416658068697274 8.052300666181877E-4 58-59 0.0 3.539472820299726E-5 0.0 1.3519458871237342 8.14078748668937E-4 60-61 0.0 3.539472820299726E-5 0.0 1.5711631362489977 8.494734768719343E-4 62-63 0.0 3.539472820299726E-5 0.0 1.8042462701477855 8.848682050749316E-4 64-65 0.0 3.539472820299726E-5 0.0 2.047638118635696 9.202629332779288E-4 66-67 0.0 3.539472820299726E-5 0.0 2.32614153750098 9.379602973794275E-4 68-69 0.0 3.539472820299726E-5 0.0 2.636544455159215 9.556576614809261E-4 70-71 0.0 3.539472820299726E-5 0.0 2.9853595015997536 9.733550255824247E-4 72-73 0.0 3.539472820299726E-5 0.0 3.3453504334703874 9.999010717346725E-4 74-75 0.0 3.539472820299726E-5 0.0 3.699368504956766 0.0010441444819884191 76-77 0.0 3.539472820299726E-5 0.0 4.109619950875657 0.0010795392101914164 78-79 0.0 3.539472820299726E-5 0.0 4.5501250407260585 0.001123782620445163 80-81 0.0 3.539472820299726E-5 0.0 5.0303430156202245 0.0011503286665974109 82-83 0.0 3.539472820299726E-5 0.0 5.534682497784733 0.001238815487104904 84-85 0.0 3.539472820299726E-5 0.0 6.020669813375987 0.001238815487104904 86-87 0.0 3.539472820299726E-5 0.0 6.54114044291901 0.0013184536255616479 88 0.0 3.539472820299726E-5 0.0 6.95504639452486 0.001398091764018392 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12825 0.0 45.63599 1 GGGCACC 6900 0.0 43.423347 94 GTATCAA 23155 0.0 41.145725 1 GTGGTAT 2690 0.0 38.30331 1 CAGTACG 1020 0.0 38.253273 9 CTGTGCG 9280 0.0 34.092415 9 GTACTAG 7035 0.0 34.040672 1 CAACGCA 27070 0.0 33.630703 5 ATCAACG 27450 0.0 33.145454 3 TAGTACT 9725 0.0 33.04007 4 ACTGTGC 9920 0.0 32.92643 8 TAGGCAT 8640 0.0 32.040268 5 TATCAAC 29925 0.0 31.79007 2 TACTGTG 11290 0.0 31.09527 7 GTACTGT 10625 0.0 30.829584 6 AACGCAG 31425 0.0 29.999876 6 CTAACGC 3375 0.0 29.690374 3 TACCCCG 355 0.0 29.126541 5 TCTAACG 3455 0.0 29.005213 2 CTAGGCA 9930 0.0 28.899508 4 >>END_MODULE