FastQCFastQC Report
Mon 16 Jan 2017
ERR1146647_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146647_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4452259
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC44150.041.20360694
GTACTAG46600.035.4166951
CTGTGCG59200.032.307329
GCATCGT49900.031.7363938
TAGGCAT61350.031.7123645
TAGTACT61050.031.5613574
CAACGCA78300.031.5094175
GTATCAA87800.030.6864681
GGTATCA57900.030.5348681
ACTGTGC68900.029.9414988
ATCAACG83450.029.6228373
CTAGTAC70250.029.168053
CTAGGCA68100.028.8460714
TCTAACG22900.028.7322442
CTAACGC23400.028.5193563
TACTGTG77350.028.1289677
CGCCTAC23600.028.076327
GTACTGT72000.028.0003056
TAACGCC24850.026.8546474
GCTATTG51500.026.6442728
Produced by FastQC (version 0.11.5)