##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146647_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4452259 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34223256104373 31.0 31.0 34.0 28.0 34.0 2 31.35675215660185 31.0 31.0 34.0 28.0 34.0 3 31.51538690808419 33.0 31.0 34.0 28.0 34.0 4 34.94097198747872 37.0 35.0 37.0 32.0 37.0 5 35.12178087573072 37.0 35.0 37.0 32.0 37.0 6 35.17466391779993 37.0 35.0 37.0 32.0 37.0 7 35.111024538329865 37.0 35.0 37.0 32.0 37.0 8 35.10231233178483 37.0 35.0 37.0 32.0 37.0 9 36.574377411556696 39.0 37.0 39.0 32.0 39.0 10-11 36.40650948204046 39.0 37.0 39.0 31.5 39.0 12-13 36.331999665787635 39.0 37.0 39.0 31.0 39.0 14-15 37.50156931571142 40.0 37.0 41.0 31.0 41.0 16-17 37.530221063060345 40.0 37.0 41.0 31.0 41.0 18-19 37.508744212769294 40.0 37.0 41.0 31.0 41.0 20-21 37.58940349157585 40.0 37.0 41.0 31.0 41.0 22-23 37.531256717095744 40.0 37.0 41.0 31.0 41.0 24-25 37.503725412200865 40.0 37.0 41.0 31.0 41.0 26-27 37.55490201715578 40.0 37.0 41.0 31.0 41.0 28-29 37.56222504126557 40.0 37.0 41.0 31.0 41.0 30-31 37.44087057828397 40.0 37.0 41.0 31.0 41.0 32-33 37.446273790451094 40.0 37.0 41.0 31.0 41.0 34-35 37.386429787665094 40.0 36.5 41.0 30.0 41.0 36-37 37.27106958512521 40.0 36.0 41.0 30.0 41.0 38-39 37.16993901298195 40.0 36.0 41.0 30.0 41.0 40-41 37.08660974125719 40.0 36.0 41.0 30.0 41.0 42-43 36.922053052169694 40.0 35.5 41.0 30.0 41.0 44-45 36.767971831827396 39.0 35.0 41.0 30.0 41.0 46-47 36.770141741529415 39.0 35.0 41.0 30.0 41.0 48-49 36.646211170554096 39.0 35.0 41.0 29.5 41.0 50-51 36.47944717501834 39.0 35.0 41.0 29.0 41.0 52-53 36.28158581969288 39.0 35.0 41.0 29.0 41.0 54-55 36.08342124750604 38.5 35.0 40.0 28.5 41.0 56-57 35.85744225571783 38.0 34.0 40.0 28.0 41.0 58-59 35.579649791263265 37.5 34.0 40.0 28.0 41.0 60-61 35.34321273762376 37.0 34.0 40.0 28.0 41.0 62-63 35.050933245348034 36.5 34.0 40.0 27.5 41.0 64-65 34.90094444191139 36.0 34.0 40.0 28.0 41.0 66-67 34.64701076464779 35.5 33.0 39.0 27.0 41.0 68-69 34.51690153245802 35.0 33.0 39.0 27.0 41.0 70-71 34.42435761261867 35.0 33.0 39.0 27.5 41.0 72-73 34.196518329234664 35.0 33.0 38.5 27.0 40.5 74-75 33.93109621430379 35.0 33.0 37.0 27.0 40.0 76-77 33.658533679195216 35.0 33.0 37.0 26.5 39.0 78-79 33.3962331257009 35.0 33.0 36.0 26.0 39.0 80-81 33.198141887073504 35.0 33.0 36.0 26.0 39.0 82-83 32.93452424937543 35.0 33.0 36.0 26.0 37.0 84-85 32.7495125508197 35.0 33.0 35.0 26.0 37.0 86-87 32.571141863040765 35.0 33.0 35.0 26.0 37.0 88-89 32.43104691348819 35.0 32.5 35.0 25.0 36.0 90-91 32.28967755469751 35.0 32.0 35.0 25.0 36.0 92-93 32.1386482682162 35.0 32.0 35.0 25.0 36.0 94-95 32.069564798453996 35.0 32.0 35.0 25.0 36.0 96-97 31.968590663750696 35.0 32.0 35.0 25.0 35.0 98-99 31.8805730978364 35.0 32.0 35.0 25.0 35.0 100 30.94723060810254 34.0 30.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3791365641129225 1101 2 0.42158380719532573 1101 3 0.466997567245361 1101 4 0.5732540310996583 1101 5 0.4844362872150114 1101 6 0.5194704877310841 1101 7 0.47559815861125543 1101 8 0.4932792647004689 1101 9 0.6269445556228632 1101 10-11 0.7113470797319437 1101 12-13 0.6125431326401554 1101 14-15 0.8018156222029234 1101 16-17 0.7785779434460878 1101 18-19 0.7656838376898207 1101 20-21 0.7684484993408418 1101 22-23 0.7779234016302823 1101 24-25 0.8167474368189431 1101 26-27 0.7583515485937511 1101 28-29 0.745826071460165 1101 30-31 0.7905757776301314 1101 32-33 0.7647501119065439 1101 34-35 0.7478692617405258 1101 36-37 0.7695013537649587 1101 38-39 0.7821397833270822 1101 40-41 0.753196475632194 1101 42-43 0.8104871893204333 1101 44-45 0.7946788583474742 1101 46-47 0.7238478370557431 1101 48-49 0.6880197723339805 1101 50-51 0.6896241648184258 1101 52-53 0.6920383977594398 1101 54-55 0.6906804891781562 1101 56-57 0.6608169269841184 1101 58-59 0.7002610997667134 1101 60-61 0.7031155979306263 1101 62-63 0.6890165444188767 1101 64-65 0.6978799666967674 1101 66-67 0.6940527356376265 1101 68-69 0.7212300731579759 1101 70-71 0.6939757688462862 1101 72-73 0.6425476743868472 1101 74-75 0.66313117908782 1101 76-77 0.6084098324362088 1101 78-79 0.5868028270950916 1101 80-81 0.5458867936710519 1101 82-83 0.5722037041425523 1101 84-85 0.569612302013816 1101 86-87 0.5412150659010351 1101 88-89 0.5375809083965137 1101 90-91 0.5104319211869708 1101 92-93 0.5125173225140358 1101 94-95 0.5618082196469842 1101 96-97 0.5374421223171737 1101 98-99 0.5144589574347549 1101 100 0.6380492954239543 1102 1 0.3671037734833611 1102 2 0.4316172492663952 1102 3 0.44894442997293993 1102 4 0.5545470369675556 1102 5 0.4060872904419668 1102 6 0.4134307244730877 1102 7 0.41703635761233215 1102 8 0.44738485872577627 1102 9 0.567052224036793 1102 10-11 0.6142206097968597 1102 12-13 0.5858973575947743 1102 14-15 0.7439585294283191 1102 16-17 0.7299345739035843 1102 18-19 0.7257289695949964 1102 20-21 0.7213847230296295 1102 22-23 0.7613489990605373 1102 24-25 0.7624462461911676 1102 26-27 0.6927038321213601 1102 28-29 0.7542629073116771 1102 30-31 0.7225515073302162 1102 32-33 0.7234317307738607 1102 34-35 0.733268576859281 1102 36-37 0.7831546344576878 1102 38-39 0.7084321079719444 1102 40-41 0.6091536127507808 1102 42-43 0.6447549707071758 1102 44-45 0.6844987715870943 1102 46-47 0.6364699031946373 1102 48-49 0.6287703112740246 1102 50-51 0.5842292067130543 1102 52-53 0.5797916960631326 1102 54-55 0.5424769716285169 1102 56-57 0.5156309460616484 1102 58-59 0.5611994311431445 1102 60-61 0.5237538652181541 1102 62-63 0.553829920965903 1102 64-65 0.5999148654829796 1102 66-67 0.615542133063613 1102 68-69 0.6006631291010436 1102 70-71 0.5289568059889973 1102 72-73 0.5698935497279578 1102 74-75 0.5311462218055496 1102 76-77 0.5171230853781879 1102 78-79 0.45753171697825934 1102 80-81 0.515183098487519 1102 82-83 0.5525515101236635 1102 84-85 0.5730588215503829 1102 86-87 0.5157944018856284 1102 88-89 0.5450648762052737 1102 90-91 0.5228894737226355 1102 92-93 0.5361490784349954 1102 94-95 0.5999959647143314 1102 96-97 0.5545172883022644 1102 98-99 0.4564568262970923 1102 100 0.5147320980481993 1103 1 0.29817005659132434 1103 2 0.33999285247482547 1103 3 0.3410187424081954 1103 4 0.4766737516661905 1103 5 0.29667992165742874 1103 6 0.37221751600269926 1103 7 0.36199206080099344 1103 8 0.36706975727120295 1103 9 0.5397980943834924 1103 10-11 0.5431930952838542 1103 12-13 0.5000379435077491 1103 14-15 0.6075296012877942 1103 16-17 0.5799151529194759 1103 18-19 0.6522822929220524 1103 20-21 0.5951096907109985 1103 22-23 0.6548567956570182 1103 24-25 0.667819897212361 1103 26-27 0.5646819889025849 1103 28-29 0.5257717962525419 1103 30-31 0.6062222430025912 1103 32-33 0.572022161708766 1103 34-35 0.5726822603298061 1103 36-37 0.6507074895716443 1103 38-39 0.6223891460953155 1103 40-41 0.5845913186258258 1103 42-43 0.5873774730929497 1103 44-45 0.5138598601894273 1103 46-47 0.5614499892339566 1103 48-49 0.5103845091827921 1103 50-51 0.5062418718544777 1103 52-53 0.4553148359249448 1103 54-55 0.4999227980888392 1103 56-57 0.5436695046256546 1103 58-59 0.5379742499914499 1103 60-61 0.5737325411151772 1103 62-63 0.5060265099348769 1103 64-65 0.4958117182746662 1103 66-67 0.6406415194338848 1103 68-69 0.5943456457670848 1103 70-71 0.5535834851474704 1103 72-73 0.48719503264735664 1103 74-75 0.4703556983286319 1103 76-77 0.3963396057339992 1103 78-79 0.4436875360966681 1103 80-81 0.3406347682106414 1103 82-83 0.37926584189277435 1103 84-85 0.37721335296811986 1103 86-87 0.4001117852485123 1103 88-89 0.43409048393073846 1103 90-91 0.4642388354440854 1103 92-93 0.3959893035808406 1103 94-95 0.3626437487731735 1103 96-97 0.36711815933454517 1103 98-99 0.3776581207847869 1103 100 0.4345057782352626 1104 1 0.33188950780250437 1104 2 0.3835825351838871 1104 3 0.32619340425484467 1104 4 0.4463735414682901 1104 5 0.3707109562775841 1104 6 0.38298194825458864 1104 7 0.42825329641823373 1104 8 0.4865266814389386 1104 9 0.5255977279153825 1104 10-11 0.6143644295884059 1104 12-13 0.5918331614827679 1104 14-15 0.7023050563299478 1104 16-17 0.6854436233362904 1104 18-19 0.6339717141982746 1104 20-21 0.6699518466977494 1104 22-23 0.6674440165052786 1104 24-25 0.7112087838876207 1104 26-27 0.572523313958996 1104 28-29 0.5809253882750269 1104 30-31 0.5901935159562015 1104 32-33 0.6456626345112184 1104 34-35 0.6307321685490237 1104 36-37 0.6199359351897371 1104 38-39 0.6243586938723524 1104 40-41 0.5848896357144397 1104 42-43 0.6435825322159943 1104 44-45 0.5981611036374588 1104 46-47 0.5681966131587473 1104 48-49 0.5889773140211503 1104 50-51 0.5247636855495941 1104 52-53 0.6231684029667548 1104 54-55 0.6492721998353801 1104 56-57 0.6015580755401118 1104 58-59 0.5875227011260336 1104 60-61 0.5829478714249987 1104 62-63 0.594470237648089 1104 64-65 0.5662187813462296 1104 66-67 0.5589917433775184 1104 68-69 0.6128024833303058 1104 70-71 0.48950598884044183 1104 72-73 0.5428705942894823 1104 74-75 0.5304429505619268 1104 76-77 0.5230971452850639 1104 78-79 0.48657767353403614 1104 80-81 0.4688432973365977 1104 82-83 0.4735143239029611 1104 84-85 0.4788294051156825 1104 86-87 0.4531077239332433 1104 88-89 0.409017634247256 1104 90-91 0.46371634274041895 1104 92-93 0.5072821143686994 1104 94-95 0.5600725315666679 1104 96-97 0.5616117585146441 1104 98-99 0.5516100741988339 1104 100 0.5987249575337259 1105 1 0.16778788311553683 1105 2 0.24340309159705242 1105 3 0.2790517363590652 1105 4 0.32949463618947306 1105 5 0.2555448492293948 1105 6 0.28226706779178556 1105 7 0.28707682737378803 1105 8 0.30223706273359596 1105 9 0.41919546326701607 1105 10-11 0.44180630651221975 1105 12-13 0.3353288979020306 1105 14-15 0.5081939014198227 1105 16-17 0.40442740096138863 1105 18-19 0.4275989045355715 1105 20-21 0.3626489742111545 1105 22-23 0.38668892609413064 1105 24-25 0.4186235625745667 1105 26-27 0.4040388932581678 1105 28-29 0.4690311510069378 1105 30-31 0.5225723441639119 1105 32-33 0.4485203148790404 1105 34-35 0.4084045895668922 1105 36-37 0.4315527339620573 1105 38-39 0.41789033265607145 1105 40-41 0.40486523802822205 1105 42-43 0.3897860583810697 1105 44-45 0.3916219699727108 1105 46-47 0.3935241085086716 1105 48-49 0.29010550470819396 1105 50-51 0.31205389860353705 1105 52-53 0.3658274454548902 1105 54-55 0.33991922272978314 1105 56-57 0.3541085518675544 1105 58-59 0.31420083922801467 1105 60-61 0.37110716839651303 1105 62-63 0.34109074388192084 1105 64-65 0.391873435242708 1105 66-67 0.3230682871485797 1105 68-69 0.2882941200214688 1105 70-71 0.31931038831478276 1105 72-73 0.2947801819179361 1105 74-75 0.23619971624178504 1105 76-77 0.308790997844973 1105 78-79 0.33924555426749947 1105 80-81 0.2711771609821483 1105 82-83 0.2596916205213944 1105 84-85 0.23936870429523793 1105 86-87 0.2349916523937594 1105 88-89 0.21136241119681642 1105 90-91 0.20961998268379745 1105 92-93 0.2814338903897422 1105 94-95 0.23294779656968956 1105 96-97 0.23747608781840768 1105 98-99 0.22091985999013986 1105 100 0.3871641512282089 1106 1 0.15485019394369104 1106 2 0.23985344092795202 1106 3 0.156598410669627 1106 4 0.31843567733086076 1106 5 0.19171258545897274 1106 6 0.20865857786875353 1106 7 0.20333249540725262 1106 8 0.20978319768362752 1106 9 0.3261033127184092 1106 10-11 0.3384862681205618 1106 12-13 0.2572366675888347 1106 14-15 0.4072481888591426 1106 16-17 0.2603936611325324 1106 18-19 0.3178374327211273 1106 20-21 0.289329535953037 1106 22-23 0.3790279385815509 1106 24-25 0.38555211030286785 1106 26-27 0.35990865094179725 1106 28-29 0.32894012399949446 1106 30-31 0.3951550657366383 1106 32-33 0.2860720552958824 1106 34-35 0.3474493584323284 1106 36-37 0.3583775472137418 1106 38-39 0.40047825116587177 1106 40-41 0.39034647971888603 1106 42-43 0.2957114318164926 1106 44-45 0.3456262070337388 1106 46-47 0.3474538353179213 1106 48-49 0.26878907336394064 1106 50-51 0.27695163576242976 1106 52-53 0.2504215108177945 1106 54-55 0.28389557426049805 1106 56-57 0.2746840841611231 1106 58-59 0.27337705000166324 1106 60-61 0.2781760241410325 1106 62-63 0.26475768141055056 1106 64-65 0.3011483726275088 1106 66-67 0.26157285141322006 1106 68-69 0.35105992284189824 1106 70-71 0.3449389817832156 1106 72-73 0.26573376036404284 1106 74-75 0.286051316129452 1106 76-77 0.2408371417843398 1106 78-79 0.27898231256552464 1106 80-81 0.27649169806520746 1106 82-83 0.2804873452746648 1106 84-85 0.2560741939472351 1106 86-87 0.25129336020840043 1106 88-89 0.34255852631542894 1106 90-91 0.26029467712846355 1106 92-93 0.2803873494843927 1106 94-95 0.27147276199870163 1106 96-97 0.27199062493522774 1106 98-99 0.3025472862790153 1106 100 0.30157919869488836 1107 1 0.18271220550130352 1107 2 0.2325916941182946 1107 3 0.24247426532643246 1107 4 0.33947823669478794 1107 5 0.25127526697608715 1107 6 0.2994529525950327 1107 7 0.27305253013821584 1107 8 0.24969597429620904 1107 9 0.34552289302033046 1107 10-11 0.3583233515967237 1107 12-13 0.3076878250921169 1107 14-15 0.47978401727228004 1107 16-17 0.3673279017377453 1107 18-19 0.33593728831469605 1107 20-21 0.2756965160809628 1107 22-23 0.3807155410932026 1107 24-25 0.443915434128634 1107 26-27 0.34590983515270324 1107 28-29 0.36910697571327944 1107 30-31 0.404125425114394 1107 32-33 0.4009947484851537 1107 34-35 0.35936038218164157 1107 36-37 0.3984441709737254 1107 38-39 0.45257367395618786 1107 40-41 0.3485056639729578 1107 42-43 0.3677593765528613 1107 44-45 0.3760951226021163 1107 46-47 0.343642432631313 1107 48-49 0.42278936686741275 1107 50-51 0.3737805065795996 1107 52-53 0.34218504609523137 1107 54-55 0.34122944780791187 1107 56-57 0.3485562073979054 1107 58-59 0.277343765653562 1107 60-61 0.3033225549452183 1107 62-63 0.33205605752225864 1107 64-65 0.32453536962466245 1107 66-67 0.2783801742825034 1107 68-69 0.2869612997250073 1107 70-71 0.28789542515328037 1107 72-73 0.25055213575516433 1107 74-75 0.2655007192399168 1107 76-77 0.2627403699317199 1107 78-79 0.2560369303847452 1107 80-81 0.23342074245221056 1107 82-83 0.2502134684608137 1107 84-85 0.26286328883994514 1107 86-87 0.1983962837375728 1107 88-89 0.30055358145312994 1107 90-91 0.25696076993096284 1107 92-93 0.25285351118458266 1107 94-95 0.2216102472194379 1107 96-97 0.31567654426307 1107 98-99 0.3209426422620254 1107 100 0.336752624920571 1108 1 0.04415020427447658 1108 2 0.078425547626221 1108 3 0.08335400744436328 1108 4 0.143854553714732 1108 5 0.0479776842391928 1108 6 0.0924518410137054 1108 7 0.09291091383744288 1108 8 0.142286033333491 1108 9 0.1187753827251612 1108 10-11 0.23575397715747215 1108 12-13 0.13923079388395365 1108 14-15 0.239411699220355 1108 16-17 0.26715494600675527 1108 18-19 0.21762406306743287 1108 20-21 0.18054015695408765 1108 22-23 0.2501732825378298 1108 24-25 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-0.9198983066469708 2215 40-41 -1.0136740686667167 2215 42-43 -1.018097868168205 2215 44-45 -0.9244350745132905 2215 46-47 -1.0721590311463984 2215 48-49 -1.078129666506534 2215 50-51 -1.053115454672188 2215 52-53 -0.9234626617372115 2215 54-55 -0.9340768357405409 2215 56-57 -0.9715039996376333 2215 58-59 -0.9988065817946747 2215 60-61 -0.9844239408282718 2215 62-63 -1.0036808807345707 2215 64-65 -1.0286049935341666 2215 66-67 -0.8999121175358411 2215 68-69 -0.9061605022711205 2215 70-71 -0.9929708829051762 2215 72-73 -0.9643597139493707 2215 74-75 -1.023799182614539 2215 76-77 -0.9791157219750701 2215 78-79 -0.956874376412884 2215 80-81 -0.8133829895962279 2215 82-83 -0.7921684249239718 2215 84-85 -0.8541433511104444 2215 86-87 -0.8004212593427162 2215 88-89 -0.8218952941367306 2215 90-91 -0.9084042872488709 2215 92-93 -0.9027157715732734 2215 94-95 -0.8188606504123612 2215 96-97 -1.1034339005429494 2215 98-99 -0.9748321959318247 2215 100 -0.5444169922711808 2216 1 -0.5144440262131482 2216 2 -0.6265362215301167 2216 3 -0.6672242550023135 2216 4 -0.5621631696239362 2216 5 -0.5365338328682583 2216 6 -0.5481470275179419 2216 7 -0.5728429405915207 2216 8 -0.6806863920304664 2216 9 -0.8190070656375994 2216 10-11 -1.1001103729756139 2216 12-13 -0.6166547365863551 2216 14-15 -0.7743600757072073 2216 16-17 -0.8219432961113924 2216 18-19 -1.0154676344892195 2216 20-21 -1.122188823738604 2216 22-23 -1.030328527792399 2216 24-25 -1.0762344643327992 2216 26-27 -1.451336653066143 2216 28-29 -1.2519528880605932 2216 30-31 -1.4734293311356694 2216 32-33 -1.664132413750913 2216 34-35 -1.2400454164163293 2216 36-37 -1.3793351748195946 2216 38-39 -1.2523347501117001 2216 40-41 -1.202706603262783 2216 42-43 -1.2266443568539032 2216 44-45 -1.0902146484293738 2216 46-47 -1.231255110938477 2216 48-49 -1.2380918387453121 2216 50-51 -1.3088118437992122 2216 52-53 -1.3015021042086161 2216 54-55 -1.2989746236782977 2216 56-57 -1.2322367877794846 2216 58-59 -1.1724817256898419 2216 60-61 -1.1426681249233681 2216 62-63 -1.282942967248566 2216 64-65 -1.3073676084575325 2216 66-67 -1.2617491678776105 2216 68-69 -1.2175985468389356 2216 70-71 -1.1809025570784328 2216 72-73 -1.205366549253462 2216 74-75 -1.1954475477204696 2216 76-77 -1.1877591386292607 2216 78-79 -1.1143171925400281 2216 80-81 -1.0810564008529369 2216 82-83 -1.146481288630639 2216 84-85 -1.161215827105238 2216 86-87 -1.1343307630007615 2216 88-89 -1.1743946931561133 2216 90-91 -1.229449086385273 2216 92-93 -1.1747287618260458 2216 94-95 -1.190409184700009 2216 96-97 -1.2629051283123331 2216 98-99 -1.2488056529991276 2216 100 -0.8205342492595662 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 1.0 2 15.0 3 26.0 4 35.0 5 28.0 6 53.0 7 167.0 8 480.0 9 819.0 10 1208.0 11 2213.0 12 3735.0 13 5655.0 14 7624.0 15 10297.0 16 12817.0 17 14846.0 18 17512.0 19 20134.0 20 22997.0 21 26535.0 22 30146.0 23 34980.0 24 41094.0 25 46415.0 26 51541.0 27 56296.0 28 64184.0 29 78940.0 30 91111.0 31 104926.0 32 129328.0 33 167011.0 34 241745.0 35 346597.0 36 549829.0 37 981469.0 38 925009.0 39 363505.0 40 936.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.713951590628625 20.845816684806902 12.382885356031439 24.057346368533032 2 15.46903147841166 19.852962977937707 40.857558727206566 23.820446816444065 3 18.24525361451277 26.545369025547217 29.404768376028827 25.804608983911187 4 11.804956240585359 16.75061693564147 40.55229273635497 30.8921340874182 5 12.71962689034558 38.826700951641655 35.634092346616065 12.8195798113967 6 29.751014902000243 39.00864615195251 18.125743179593517 13.114595766453721 7 26.613789698716733 32.19228511067058 23.822441917766003 17.37148327284668 8 25.90857533034304 35.49246550573987 20.0220341000721 18.576925063844985 9 26.001498575544645 15.107492248824633 21.37907133237561 37.511937843255104 10-11 25.213468892383016 26.16385790534832 28.216009529607582 20.40666367266108 12-13 25.386962170087713 24.19504636313733 27.899605232777 22.518386233997955 14-15 22.811962806666607 25.507207087114054 25.271472319779026 26.409357786440314 16-17 21.812127040998764 28.053008019348496 26.567056703244575 23.567808236408165 18-19 21.213621328609953 28.709051375848617 28.143384605225375 21.93394269031606 20-21 23.0138015494526 27.84251892455838 27.120395623079958 22.023283902909053 22-23 23.46196011912289 27.523595590413656 26.746531890124654 22.267912400338798 24-25 22.826580117694622 27.560981088001434 27.579836934549213 22.032601859754728 26-27 22.78334573756553 27.700643930702963 26.642134475802987 22.87387585592852 28-29 21.877788020172126 28.29171686787581 27.179440010565838 22.651055101386227 30-31 22.69384571048777 27.853645738924214 27.16301743495612 22.289491115631893 32-33 23.0960027080031 28.123980090696538 26.19528035671247 22.58473684458789 34-35 23.002179862829237 27.921381186245185 27.23434816935322 21.842090781572352 36-37 22.636997490181475 27.533170833992692 26.92935209574396 22.90047958008188 38-39 22.792854745052747 27.688654701454258 27.459015795073192 22.059474758419803 40-41 22.44046977950489 26.811883683379854 28.08638852148252 22.661258015632736 42-43 22.356628655500497 27.08377301305668 27.397377208193717 23.16222112324911 44-45 22.57422922212486 28.019133912564094 27.254413170256708 22.152223695054335 46-47 22.567579103438206 27.678217210122547 26.81717193274102 22.937031753698236 48-49 23.150365662388257 27.304079668313037 27.061611475554752 22.483943193743947 50-51 22.76232271166489 27.194107068844094 27.028463854914204 23.015106364576816 52-53 22.22521521462601 27.823940754027397 26.87028575613697 23.08055827520962 54-55 22.274229452189427 27.691927346183043 26.83473846831937 23.199104733308157 56-57 23.329162165935486 27.14752690133405 26.569059627326542 22.954251305403922 58-59 22.28673371988564 27.560980157425973 27.430765301228423 22.721520821459965 60-61 22.349920162891067 28.120554933052745 26.755281963822153 22.77424294023404 62-63 22.675111233658914 27.470982096477194 26.470741181471897 23.383165488391995 64-65 21.942927663740154 27.694862595710497 26.83556422426443 23.526645516284923 66-67 22.693736718437616 27.302365859027866 26.741698602319136 23.262198820215378 68-69 22.106159258541737 27.40400322324329 27.070717620864603 23.41911989735037 70-71 22.170189524633464 27.02307092823145 27.203308518843016 23.603431028292068 72-73 22.747045168431885 27.240673568059147 27.5148821172786 22.49739914623037 74-75 22.900404894676843 27.459923289025163 27.10448079698547 22.535191019312524 76-77 22.639866894409547 27.421692419522515 26.75884877231792 23.179591913750023 78-79 22.44960924781116 27.353630365872 26.8835888573319 23.313171528984938 80-81 22.08612081218331 27.69535656985811 27.434138002876345 22.784384615082235 82-83 22.853426064783758 26.798960151689283 28.386967147653223 21.960646635873733 84-85 23.131232174333196 27.35223551298498 27.024109589902682 22.49242272277914 86-87 21.87644321569572 27.986737690100146 27.44805321995461 22.688765874249526 88-89 22.96889639025977 26.79540792110922 27.215664138248446 23.020031550382562 90-91 22.632833288238185 26.857950959616872 27.450062038118713 23.05915371402623 92-93 22.93599877265859 27.06644962071242 27.340860608023675 22.656690998605313 94-95 21.84659596944525 27.162578227079727 27.904411624317742 23.08641417915728 96-97 22.2076565136365 27.053589889970624 27.446684321473526 23.29206927491935 98-99 21.589117837494225 27.813779482678356 27.285372615680252 23.311730064147167 100 22.38636164028921 27.205782007299124 26.52588630106199 23.88197005134968 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1797.0 1 1231.0 2 779.5 3 872.5 4 778.5 5 678.5 6 609.0 7 586.0 8 636.0 9 626.5 10 536.0 11 464.5 12 456.0 13 481.0 14 522.5 15 824.0 16 1004.0 17 1126.0 18 1571.0 19 2063.0 20 2731.5 21 3572.5 22 4643.0 23 6326.0 24 8536.5 25 10983.5 26 14502.5 27 18252.0 28 23076.0 29 32727.0 30 45401.5 31 57071.0 32 72379.0 33 86717.0 34 96298.0 35 108123.5 36 119623.5 37 138711.0 38 146199.5 39 136198.5 40 128025.5 41 120201.5 42 119076.5 43 125562.5 44 135909.0 45 158763.5 46 180626.5 47 194715.0 48 203610.0 49 199324.0 50 190299.5 51 195703.0 52 199301.5 53 190140.5 54 178919.5 55 154168.0 56 134790.0 57 111254.0 58 86337.0 59 82352.5 60 68214.5 61 46043.5 62 33750.0 63 27515.0 64 19420.0 65 8834.5 66 4339.5 67 2550.5 68 1489.5 69 795.5 70 420.0 71 241.5 72 143.5 73 95.0 74 75.0 75 61.5 76 45.5 77 38.5 78 31.0 79 23.5 80 26.5 81 26.0 82 24.5 83 20.0 84 17.0 85 18.0 86 13.5 87 8.5 88 7.5 89 6.5 90 7.5 91 7.0 92 6.0 93 6.0 94 5.0 95 5.5 96 6.0 97 4.0 98 3.5 99 3.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05987971499411872 2 0.007164902131704377 3 0.007748875346200659 4 0.009366031940190362 5 0.003661062844726688 6 0.0023583533662349834 7 8.310388052447084E-4 8 0.0015497750692401318 9 6.962757557455665E-4 10-11 6.625849933707811E-4 12-13 3.3690762374785475E-4 14-15 2.1337482837364134E-4 16-17 6.064337227461385E-4 18-19 0.00178561040586363 20-21 9.321110923690648E-4 22-23 0.0011230254124928493 24-25 0.0013251699867415621 26-27 0.0033578459833536185 28-29 0.003930588943724972 30-31 0.005076074864467679 32-33 0.006064337227461385 34-35 0.003234313187979406 36-37 0.007715184583825874 38-39 0.006053106973336457 40-41 0.005738659857838458 42-43 0.014116429435035114 44-45 0.020113385137746926 46-47 0.018170551174134297 48-49 0.021416094616238633 50-51 0.018507458797882154 52-53 0.025178229748089677 54-55 0.019046510995878722 56-57 0.014992389256779535 58-59 0.017294591352389876 60-61 0.015610053233650605 62-63 0.010814734722306138 64-65 0.010949497771805278 66-67 0.013453844441664331 68-69 0.004975002577343322 70-71 0.00802963169932387 72-73 0.007984710682824157 74-75 0.01087088599293078 76-77 0.010219531253684928 78-79 0.011780536577049987 80-81 0.009691709309813288 82-83 0.005918343923837315 84-85 0.010376754811433925 86-87 0.008871900758693508 88-89 0.007456888738952518 90-91 0.012589114874044838 92-93 0.008085782969948514 94-95 0.007097520606954807 96-97 0.009040354570567436 98-99 0.008680986438569724 100 0.26334945922957315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4452259.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.12720660304892 #Duplication Level Percentage of deduplicated Percentage of total 1 75.79566363541034 52.39542499740213 2 15.000555987627276 20.738930658346266 3 4.900515857347775 10.162769163971912 4 2.0035824685970036 5.540082370118074 5 0.9642158809558186 3.332677520638684 6 0.49282103940206223 2.04404050854454 7 0.2980571802359431 1.4422702194384558 8 0.16742306014985348 0.9258790775274887 9 0.10750556946343438 0.6688403740149529 >10 0.2674417108982502 2.5028900357035626 >50 0.0017242559148050165 0.08222023847940989 >100 3.596450518909971E-4 0.052104622768618755 >500 8.913929711373652E-5 0.042579078681354875 >1k 4.456964841359953E-5 0.06929113436458217 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.738152474957094E-5 0.0 5 0.0 0.0 0.0 6.738152474957094E-5 0.0 6 0.0 0.0 0.0 6.738152474957094E-5 2.2460508249856984E-5 7 0.0 0.0 0.0 6.738152474957094E-5 2.2460508249856984E-5 8 0.0 0.0 0.0 6.738152474957094E-5 2.2460508249856984E-5 9 0.0 0.0 0.0 6.738152474957094E-5 2.2460508249856984E-5 10-11 0.0 0.0 0.0 8.984203299942794E-5 2.2460508249856984E-5 12-13 0.0 0.0 0.0 1.5722355774899888E-4 2.2460508249856984E-5 14-15 0.0 0.0 0.0 1.7968406599885587E-4 2.2460508249856984E-5 16-17 0.0 0.0 0.0 1.7968406599885587E-4 2.2460508249856984E-5 18-19 0.0 0.0 0.0 1.7968406599885587E-4 2.2460508249856984E-5 20-21 0.0 0.0 0.0 2.3583533662349833E-4 2.2460508249856984E-5 22-23 0.0 0.0 0.0 2.6952609899828377E-4 2.2460508249856984E-5 24-25 0.0 0.0 0.0 3.5936813199771174E-4 2.2460508249856984E-5 26-27 0.0 0.0 0.0 8.871900758693509E-4 2.2460508249856984E-5 28-29 0.0 0.0 0.0 0.0028749450559816935 2.2460508249856984E-5 30-31 0.0 0.0 0.0 0.009848932867562287 2.2460508249856984E-5 32-33 0.0 0.0 0.0 0.021719311477611702 2.2460508249856984E-5 34-35 0.0 0.0 0.0 0.040821973744115066 2.2460508249856984E-5 36-37 0.0 0.0 0.0 0.07429936129052689 2.2460508249856984E-5 38-39 0.0 0.0 0.0 0.1239258542685859 2.2460508249856984E-5 40-41 0.0 0.0 0.0 0.17789845559299222 2.2460508249856984E-5 42-43 0.0 0.0 0.0 0.23451016663675678 2.2460508249856984E-5 44-45 0.0 0.0 0.0 0.30485647847530883 2.2460508249856984E-5 46-47 0.0 0.0 0.0 0.393081354880747 2.2460508249856984E-5 48-49 0.0 0.0 0.0 0.49680398197858655 2.2460508249856984E-5 50-51 0.0 0.0 0.0 0.6041090601422783 2.2460508249856984E-5 52-53 0.0 0.0 0.0 0.7124810124478382 3.369076237478548E-5 54-55 0.0 0.0 0.0 0.8362945641751749 4.492101649971397E-5 56-57 0.0 0.0 0.0 0.9870270350399651 4.492101649971397E-5 58-59 0.0 0.0 0.0 1.1698218814314263 4.492101649971397E-5 60-61 0.0 0.0 0.0 1.3628025683141973 4.492101649971397E-5 62-63 0.0 0.0 0.0 1.5602753568469399 4.492101649971397E-5 64-65 0.0 0.0 0.0 1.7663729805476276 4.492101649971397E-5 66-67 0.0 0.0 0.0 2.00470143358686 5.615127062464246E-5 68-69 0.0 0.0 0.0 2.275260715964637 6.738152474957094E-5 70-71 0.0 0.0 0.0 2.577702689803086 6.738152474957094E-5 72-73 0.0 0.0 0.0 2.8977761626176735 6.738152474957094E-5 74-75 0.0 0.0 0.0 3.209090037214816 6.738152474957094E-5 76-77 0.0 0.0 0.0 3.5703223913972657 6.738152474957094E-5 78-79 0.0 0.0 0.0 3.961067853420028 8.984203299942794E-5 80-81 0.0 0.0 0.0 4.39169194784041 8.984203299942794E-5 82-83 0.0 0.0 0.0 4.846607081933014 8.984203299942794E-5 84-85 0.0 0.0 0.0 5.286866734392586 8.984203299942794E-5 86-87 0.0 0.0 0.0 5.751473577795002 8.984203299942794E-5 88 0.0 0.0 0.0 6.128304754956978 8.984203299942794E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 4415 0.0 41.203606 94 GTACTAG 4660 0.0 35.416695 1 CTGTGCG 5920 0.0 32.30732 9 GCATCGT 4990 0.0 31.736393 8 TAGGCAT 6135 0.0 31.712364 5 TAGTACT 6105 0.0 31.561357 4 CAACGCA 7830 0.0 31.509417 5 GTATCAA 8780 0.0 30.686468 1 GGTATCA 5790 0.0 30.534868 1 ACTGTGC 6890 0.0 29.941498 8 ATCAACG 8345 0.0 29.622837 3 CTAGTAC 7025 0.0 29.16805 3 CTAGGCA 6810 0.0 28.846071 4 TCTAACG 2290 0.0 28.732244 2 CTAACGC 2340 0.0 28.519356 3 TACTGTG 7735 0.0 28.128967 7 CGCCTAC 2360 0.0 28.07632 7 GTACTGT 7200 0.0 28.000305 6 TAACGCC 2485 0.0 26.854647 4 GCTATTG 5150 0.0 26.644272 8 >>END_MODULE