FastQCFastQC Report
Mon 16 Jan 2017
ERR1146647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4452259
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153180.3440500653713093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135940.3053281491485558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93780.21063464636715878No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94650.048.218681
GGGCACC44400.040.14081294
GTGGTAT22550.039.2261351
GTATCAA165600.038.7541541
GTACTAG43650.031.1514171
CAACGCA202450.030.8762635
ATCAACG204800.030.5305653
CTGTGCG61850.030.4767539
TAGTACT64900.030.2295134
TAGGCAT52850.029.4356675
CTAACGC23350.029.216013
TATCAAC222450.028.786572
TCTAACG24050.028.7585162
AACGCAG227500.028.4045416
ACTGTGC71350.028.3896168
TAACGCC24050.028.365654
CGCCTAC24150.027.8282157
TACTGTG80400.026.9470567
GTACTGT74450.026.8281486
TCAACGC239400.026.3735284
Produced by FastQC (version 0.11.5)