##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146647_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4452259
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13557522147746	33.0	31.0	34.0	30.0	34.0
2	32.22414576510486	33.0	31.0	34.0	30.0	34.0
3	32.37601249163627	34.0	31.0	34.0	30.0	34.0
4	35.97589717938691	37.0	35.0	37.0	35.0	37.0
5	35.88799079298846	37.0	35.0	37.0	35.0	37.0
6	35.92614490756266	37.0	35.0	37.0	35.0	37.0
7	35.85155400887505	37.0	35.0	37.0	35.0	37.0
8	35.86243253144078	37.0	35.0	37.0	35.0	37.0
9	37.57708457661605	39.0	37.0	39.0	35.0	39.0
10-11	37.514768907199695	39.0	37.0	39.0	34.5	39.0
12-13	37.47814985606183	39.0	37.0	39.0	34.0	39.0
14-15	38.68917789823099	40.0	38.0	41.0	34.0	41.0
16-17	38.69701077587804	40.0	38.0	41.0	34.0	41.0
18-19	38.77368162993213	40.0	38.0	41.0	34.0	41.0
20-21	38.778447862085294	40.0	38.0	41.0	34.0	41.0
22-23	38.740592359968275	40.0	38.0	41.0	34.0	41.0
24-25	38.6647273889502	40.0	38.0	41.0	34.0	41.0
26-27	38.516815733316506	40.0	38.0	41.0	34.0	41.0
28-29	38.45555829074634	40.0	38.0	41.0	34.0	41.0
30-31	38.30839367161704	40.0	37.5	41.0	34.0	41.0
32-33	38.14726883588757	40.0	37.0	41.0	33.5	41.0
34-35	38.04239971214612	40.0	37.0	41.0	33.0	41.0
36-37	38.02477551283517	40.0	37.0	41.0	33.0	41.0
38-39	37.930719776185526	40.0	37.0	41.0	33.0	41.0
40-41	37.81529129370057	40.0	37.0	41.0	33.0	41.0
42-43	37.67416821438286	40.0	37.0	41.0	33.0	41.0
44-45	37.45788542400611	40.0	36.0	41.0	32.0	41.0
46-47	37.19408091487939	40.0	36.0	41.0	31.0	41.0
48-49	36.906283753932556	39.5	35.5	41.0	31.0	41.0
50-51	36.69334814079774	39.0	35.0	41.0	30.0	41.0
52-53	36.521774339722825	39.0	35.0	41.0	30.0	41.0
54-55	36.34391249475828	39.0	35.0	41.0	30.0	41.0
56-57	36.126471865181244	38.5	35.0	41.0	29.5	41.0
58-59	35.86359284129696	38.0	35.0	40.0	29.0	41.0
60-61	35.68731794354282	37.0	35.0	40.0	29.0	41.0
62-63	35.47848631447542	37.0	35.0	40.0	29.0	41.0
64-65	35.2365148793006	36.5	34.0	40.0	29.0	41.0
66-67	34.98997868273162	36.0	34.0	39.5	29.0	41.0
68-69	34.73809216849244	35.5	34.0	39.0	28.5	41.0
70-71	34.50982299098054	35.0	34.0	39.0	28.5	41.0
72-73	34.265089699408776	35.0	34.0	38.0	28.0	40.5
74-75	33.9921215275212	35.0	34.0	37.0	28.0	40.0
76-77	33.73740521384762	35.0	34.0	37.0	28.0	39.0
78-79	33.507391528659944	35.0	33.5	36.0	27.0	39.0
80-81	33.364094047538565	35.0	33.0	36.0	27.0	39.0
82-83	33.19210551317882	35.0	33.0	36.0	27.0	37.0
84-85	33.04601304641082	35.0	33.0	35.0	27.0	37.0
86-87	32.89114166538829	35.0	33.0	35.0	27.0	37.0
88-89	32.774145664032574	35.0	33.0	35.0	27.0	36.0
90-91	32.70375959709442	35.0	33.0	35.0	27.0	36.0
92-93	32.63117745396214	35.0	33.0	35.0	27.0	36.0
94-95	32.573385443209844	35.0	33.0	35.0	27.0	36.0
96-97	32.478357615763144	35.0	33.0	35.0	27.0	35.0
98-99	32.40023614978374	35.0	33.0	35.0	27.0	35.0
100	31.201339589633037	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36938672139726947
1101	2	0.4210741588148892
1101	3	0.3883577656411532
1101	4	0.29463483189498874
1101	5	0.2460885008932081
1101	6	0.28978415738941266
1101	7	0.33042526297715824
1101	8	0.3169094952094298
1101	9	0.386292583444245
1101	10-11	0.4241168086774678
1101	12-13	0.3824497607906423
1101	14-15	0.5177686001535875
1101	16-17	0.5143466668444319
1101	18-19	0.48975783697532194
1101	20-21	0.47754417192826537
1101	22-23	0.48829001466046407
1101	24-25	0.4749061070993932
1101	26-27	0.5390871897352554
1101	28-29	0.5465062136496996
1101	30-31	0.5565939601764782
1101	32-33	0.6124171558791005
1101	34-35	0.6012900599670488
1101	36-37	0.5319685351547676
1101	38-39	0.5816573869496224
1101	40-41	0.5628224385063305
1101	42-43	0.5008391433694968
1101	44-45	0.4837950293776103
1101	46-47	0.5394473518455669
1101	48-49	0.5420839674007141
1101	50-51	0.5785593612108855
1101	52-53	0.5574081344111832
1101	54-55	0.5816219567102721
1101	56-57	0.5860047132568269
1101	58-59	0.4739876089235935
1101	60-61	0.5240302545023425
1101	62-63	0.5446485908440479
1101	64-65	0.570085611303881
1101	66-67	0.5706937004552586
1101	68-69	0.6047952133081793
1101	70-71	0.5477539790756865
1101	72-73	0.5387125736490717
1101	74-75	0.5119781646796469
1101	76-77	0.48384472500280395
1101	78-79	0.455716053933088
1101	80-81	0.4864407914300557
1101	82-83	0.45941904320429927
1101	84-85	0.4603657968870891
1101	86-87	0.43297206159567736
1101	88-89	0.4815021605973513
1101	90-91	0.4191056188360136
1101	92-93	0.4190740008650806
1101	94-95	0.48729085049593124
1101	96-97	0.477680066615612
1101	98-99	0.529527168179051
1101	100	0.8568613219295003
1102	1	0.3116418017851075
1102	2	0.3463740552361614
1102	3	0.3024488816251818
1102	4	0.23554768013655547
1102	5	0.23827429820203605
1102	6	0.216951636716459
1102	7	0.270065103526413
1102	8	0.2652640426017996
1102	9	0.34652557344585944
1102	10-11	0.28785351281023
1102	12-13	0.323347706545583
1102	14-15	0.4248120593689606
1102	16-17	0.4429822345658607
1102	18-19	0.42255092878753686
1102	20-21	0.43826427743459107
1102	22-23	0.5054704126025911
1102	24-25	0.48297596188984215
1102	26-27	0.5434505346364773
1102	28-29	0.5375291949021133
1102	30-31	0.5830422977929501
1102	32-33	0.5397530063373992
1102	34-35	0.559504683445347
1102	36-37	0.5225171577124144
1102	38-39	0.582653344400974
1102	40-41	0.5655094354008412
1102	42-43	0.5764134519046635
1102	44-45	0.6221142821474643
1102	46-47	0.6240229967972795
1102	48-49	0.6171000717007686
1102	50-51	0.5728756864804581
1102	52-53	0.6292265707686511
1102	54-55	0.5315756898242157
1102	56-57	0.49760700845081374
1102	58-59	0.42900342713342354
1102	60-61	0.5001283895458357
1102	62-63	0.551559850581377
1102	64-65	0.4756400060835304
1102	66-67	0.5031698535454225
1102	68-69	0.5643973978180767
1102	70-71	0.5277444771282802
1102	72-73	0.4673177384217979
1102	74-75	0.537449914559879
1102	76-77	0.46962245306654893
1102	78-79	0.48885262988805067
1102	80-81	0.5131873781040213
1102	82-83	0.383014709547119
1102	84-85	0.41860279168282233
1102	86-87	0.4636427157034504
1102	88-89	0.44062860286141614
1102	90-91	0.43951181301763853
1102	92-93	0.37915814187567065
1102	94-95	0.38983815563155133
1102	96-97	0.4192321975304978
1102	98-99	0.45755407895102707
1102	100	0.7118136961148736
1103	1	0.2588120016034168
1103	2	0.3466625955715017
1103	3	0.22961129470652963
1103	4	0.17526279012076884
1103	5	0.19767857155227375
1103	6	0.2584943369715731
1103	7	0.20759410316000526
1103	8	0.21905503245749003
1103	9	0.2268579176159662
1103	10-11	0.2656365719339462
1103	12-13	0.2745298872996855
1103	14-15	0.39258189296299406
1103	16-17	0.34437685382972916
1103	18-19	0.3255064723893639
1103	20-21	0.33449007171156353
1103	22-23	0.303158512973539
1103	24-25	0.33029859465072775
1103	26-27	0.33125243692990125
1103	28-29	0.36768615405751603
1103	30-31	0.3159284440506198
1103	32-33	0.3815362103732909
1103	34-35	0.3435733314651159
1103	36-37	0.32847293381576037
1103	38-39	0.3545479932434432
1103	40-41	0.3917280390985809
1103	42-43	0.3169487602626617
1103	44-45	0.41105225468844253
1103	46-47	0.3802062758573328
1103	48-49	0.3555692808425235
1103	50-51	0.3965107260630347
1103	52-53	0.3808882457608931
1103	54-55	0.3965082483359481
1103	56-57	0.29191943453299274
1103	58-59	0.35489297470015657
1103	60-61	0.3533257668187417
1103	62-63	0.30976288905969085
1103	64-65	0.372097705712676
1103	66-67	0.401239403139094
1103	68-69	0.41304407394072484
1103	70-71	0.4527402467013957
1103	72-73	0.38031363272945384
1103	74-75	0.3478050136114845
1103	76-77	0.3196737455594061
1103	78-79	0.30955292260149037
1103	80-81	0.3119875189806791
1103	82-83	0.2893783721750438
1103	84-85	0.30239126850444364
1103	86-87	0.2999259291402936
1103	88-89	0.29444110025322345
1103	90-91	0.25585222090875703
1103	92-93	0.18970884944609878
1103	94-95	0.1914490425720743
1103	96-97	0.30815932458684614
1103	98-99	0.2753197852993239
1103	100	0.4990569298394263
1104	1	0.22940922163474653
1104	2	0.2592230072268009
1104	3	0.2173016267857406
1104	4	0.16939706242175134
1104	5	0.17008293314506062
1104	6	0.17859370837923905
1104	7	0.19561884512493322
1104	8	0.226830835555198
1104	9	0.2029273242239995
1104	10-11	0.30012713995588314
1104	12-13	0.31423571667532
1104	14-15	0.2884690698358128
1104	16-17	0.27435639533157996
1104	18-19	0.32689012058288824
1104	20-21	0.29736918708643145
1104	22-23	0.383283863007712
1104	24-25	0.3955253292837497
1104	26-27	0.3751748693015031
1104	28-29	0.3612737808189692
1104	30-31	0.3830551335437562
1104	32-33	0.39416916695877546
1104	34-35	0.46259759692325275
1104	36-37	0.46566149641544996
1104	38-39	0.4461151920730444
1104	40-41	0.4856812060675395
1104	42-43	0.5046601203193077
1104	44-45	0.41246401296213264
1104	46-47	0.47080597394233337
1104	48-49	0.5293475248776858
1104	50-51	0.5330981286716181
1104	52-53	0.5154925686363612
1104	54-55	0.5578490100081339
1104	56-57	0.47274580357122176
1104	58-59	0.5233312814435322
1104	60-61	0.5007834011071495
1104	62-63	0.4894282617529555
1104	64-65	0.4846700709748717
1104	66-67	0.39869810178975484
1104	68-69	0.46759448186438135
1104	70-71	0.42741006242481205
1104	72-73	0.37677508307495344
1104	74-75	0.43946259226193973
1104	76-77	0.38657507681278247
1104	78-79	0.4128189636778643
1104	80-81	0.3448711956481674
1104	82-83	0.3241377144100852
1104	84-85	0.4430300705310586
1104	86-87	0.43350921475926896
1104	88-89	0.4317520645126649
1104	90-91	0.37492724825921897
1104	92-93	0.35199145800331166
1104	94-95	0.4067830139769413
1104	96-97	0.37041621560469906
1104	98-99	0.29969718341211404
1104	100	0.5490819559981759
1105	1	0.2170925410847815
1105	2	0.21010548454593447
1105	3	0.18481576071730643
1105	4	0.16925726715271594
1105	5	0.17750782905532247
1105	6	0.14452377295275198
1105	7	0.21633041529175046
1105	8	0.13830303195582871
1105	9	0.16827340674590374
1105	10-11	0.20888321400782672
1105	12-13	0.16344668260814643
1105	14-15	0.2750145625231539
1105	16-17	0.3212954344464407
1105	18-19	0.24304464929349479
1105	20-21	0.2607770099527116
1105	22-23	0.2615792871533813
1105	24-25	0.27183356980368956
1105	26-27	0.29177179142609333
1105	28-29	0.2869157761576062
1105	30-31	0.31198441725835124
1105	32-33	0.2468616299642079
1105	34-35	0.24228047161786748
1105	36-37	0.3413824962018168
1105	38-39	0.30631364624180435
1105	40-41	0.33598260571737626
1105	42-43	0.29035613804374094
1105	44-45	0.33792529360927404
1105	46-47	0.33473268832835856
1105	48-49	0.33496268442362975
1105	50-51	0.3000857689502183
1105	52-53	0.32986962412108767
1105	54-55	0.307710701922808
1105	56-57	0.25389574125821923
1105	58-59	0.2169326603952726
1105	60-61	0.1210255067423347
1105	62-63	0.22667511465350998
1105	64-65	0.1780057033192648
1105	66-67	0.2103038341656216
1105	68-69	0.18972621187370464
1105	70-71	0.22338748417664078
1105	72-73	0.16906761351103938
1105	74-75	0.1871342530664748
1105	76-77	0.15062631659035475
1105	78-79	0.17821413638451133
1105	80-81	0.053707809446322585
1105	82-83	0.08709729573112668
1105	84-85	0.10848916589295499
1105	86-87	0.14981426833199407
1105	88-89	0.13069525426978856
1105	90-91	0.1268115975052453
1105	92-93	0.16685263552738405
1105	94-95	0.16342851626973953
1105	96-97	0.14754290581085883
1105	98-99	0.13638625931289283
1105	100	0.30141031706398635
1106	1	0.12016751228301814
1106	2	0.1925693881167092
1106	3	0.13467789459468804
1106	4	0.13664224556520566
1106	5	0.056497060667453525
1106	6	0.12255284997183225
1106	7	0.1266883151889644
1106	8	0.11622394144429649
1106	9	0.08433561400670442
1106	10-11	0.1399893808400634
1106	12-13	0.1280726504289973
1106	14-15	0.20975575767349852
1106	16-17	0.14745071473961247
1106	18-19	0.19757529041591226
1106	20-21	0.1984495536233979
1106	22-23	0.21452582048742386
1106	24-25	0.23788255471282582
1106	26-27	0.25673631559911314
1106	28-29	0.20108937748981504
1106	30-31	0.2108336379667861
1106	32-33	0.2339186442550627
1106	34-35	0.2151000349249088
1106	36-37	0.20806618353375939
1106	38-39	0.25352820782820373
1106	40-41	0.23067248311878075
1106	42-43	0.23372720720065132
1106	44-45	0.20166847426474277
1106	46-47	0.1316087737492211
1106	48-49	0.2046172438390883
1106	50-51	0.19478837070218447
1106	52-53	0.19582703168934046
1106	54-55	0.17029714837506305
1106	56-57	0.10153865133028006
1106	58-59	0.1165213705580328
1106	60-61	0.1333916974296585
1106	62-63	0.1972755976470708
1106	64-65	0.19263353653413162
1106	66-67	0.1944149608357506
1106	68-69	0.1825653989569389
1106	70-71	0.19484393824156854
1106	72-73	0.22558099121374653
1106	74-75	0.17655873512210718
1106	76-77	0.15445543873340029
1106	78-79	0.13902680836898185
1106	80-81	0.06187810860910048
1106	82-83	0.13441069365773473
1106	84-85	0.09379258320193884
1106	86-87	0.09292470987075774
1106	88-89	0.12427869980108142
1106	90-91	0.15238722560071238
1106	92-93	0.15585037705011473
1106	94-95	0.16633089209801
1106	96-97	0.07448633195556909
1106	98-99	0.09060944805118254
1106	100	0.23750149617278637
1107	1	0.10100068997056866
1107	2	0.19637902757309433
1107	3	0.19591398780677594
1107	4	0.12167841175377703
1107	5	0.06212189252327249
1107	6	0.02623170448231349
1107	7	0.12712155997273555
1107	8	0.12490646942187311
1107	9	0.10236496163531683
1107	10-11	0.19884572932708977
1107	12-13	0.09867696848208851
1107	14-15	0.16976726838025513
1107	16-17	0.11958237254820858
1107	18-19	0.17153690502154006
1107	20-21	0.1630076915299199
1107	22-23	0.16585327366750846
1107	24-25	0.17331790772511368
1107	26-27	0.1881182360227882
1107	28-29	0.23286467669936428
1107	30-31	0.15378211493526095
1107	32-33	0.3189347860966123
1107	34-35	0.23341440978035166
1107	36-37	0.1660512328710695
1107	38-39	0.23789294444750197
1107	40-41	0.24662527664477807
1107	42-43	0.2519401644529822
1107	44-45	0.21380903659837713
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2216	74-75	-0.7258094306551968
2216	76-77	-0.8073438357251419
2216	78-79	-0.694012872407967
2216	80-81	-0.7271352537307081
2216	82-83	-0.7005352324327063
2216	84-85	-0.6683119236216086
2216	86-87	-0.7086651978514453
2216	88-89	-0.6767431328839706
2216	90-91	-0.6560191459516815
2216	92-93	-0.6770320651125346
2216	94-95	-0.7103929332632859
2216	96-97	-0.6958522540548842
2216	98-99	-0.6326426937388305
2216	100	-0.1450758275246251
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	9.0
12	12.0
13	76.0
14	262.0
15	807.0
16	1954.0
17	4055.0
18	6916.0
19	11281.0
20	16342.0
21	23403.0
22	32776.0
23	39211.0
24	39875.0
25	37324.0
26	36770.0
27	41907.0
28	52208.0
29	64765.0
30	81499.0
31	100839.0
32	127923.0
33	163800.0
34	233288.0
35	343178.0
36	541818.0
37	1035411.0
38	1011457.0
39	401768.0
40	1323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.61481126771825	21.626010526341798	13.82024271274425	24.9389354931957
2	14.135317606410327	20.877702557154283	40.13727944732422	24.849700389111177
3	16.89362635911343	26.90537545097893	30.364900155179654	25.83609803472799
4	11.329105287425365	17.65262745903823	39.18128839024394	31.836978863292458
5	12.090518853377544	38.912419781642996	35.59834117500927	13.398720189970186
6	29.47995613013529	39.55591981508712	17.406354841441164	13.557769213336421
7	25.571154392549396	32.96226294316026	23.80921577941964	17.6573668848707
8	26.265767557547754	34.988553001970466	19.599758235089197	19.14592120539259
9	25.98815567557952	14.251214945042506	21.425775095294323	38.334854284083654
10-11	25.186450294109125	26.202136937675906	27.823617179503707	20.78779558871126
12-13	25.695216370520324	24.172344885627414	28.27939465173428	21.85304409211798
14-15	22.855056401372735	25.05265236277478	26.385808391223232	25.706482844629257
16-17	21.09338203370469	27.471335337858825	27.69213336420904	23.74314926422744
18-19	20.571892285133295	27.11777819529409	30.72749996658996	21.582829552982652
20-21	22.084901170394623	26.40091243568714	30.082829862323823	21.43135653159441
22-23	22.419618894588115	26.098807369472443	29.795964250956647	21.68560948498279
24-25	21.893245653498596	26.152297069869473	30.519877662103667	21.434579614528264
26-27	21.90594707091389	26.32336753095451	29.58893451616359	22.181750881968007
28-29	21.057714746603914	26.86121809175971	30.168988371970272	21.9120787896661
30-31	21.962087111284408	26.435602690679044	29.970931610223033	21.63137858781351
32-33	22.400471311305115	26.699333978548868	29.239595001099445	21.660599709046576
34-35	22.19569885759117	26.605078455678346	29.952704907778276	21.24651777895221
36-37	21.948251438202497	26.112789035857976	29.699541289040017	22.239418236899517
38-39	22.067584118533983	26.184617741241016	30.31922671165357	21.428571428571427
40-41	21.668483347442276	25.579127359841376	30.68148101896139	22.070908273754963
42-43	21.88984288649874	25.769278022684666	29.793471134540916	22.54740795627568
44-45	22.09024677135809	26.76591815525557	29.401422963039664	21.742412110346677
46-47	22.177528306417035	26.571679679910805	28.655969924481035	22.594822089191126
48-49	22.9803454830458	26.454132609985177	28.473017854531825	22.0925040524372
50-51	22.41427329362465	26.51285560880443	28.1818510558348	22.891020041736116
52-53	21.821102501000052	27.100916635802186	27.968139319837416	23.10984154336035
54-55	21.92746423781725	27.1032637589143	27.777539446829124	23.191732556439327
56-57	22.95239338052885	26.55094863079619	27.520546311434263	22.9761116772407
58-59	21.920268106624984	26.94030456677212	28.3266683639326	22.812758962670294
60-61	22.101488255737145	27.376338618216057	27.653624822814667	22.868548303232135
62-63	22.447346392022567	26.756080452642127	27.320849932584785	23.47572322275052
64-65	21.694942777892514	26.927233415235623	27.695439380008768	23.6823844268631
66-67	22.310779164204895	26.53505759950892	27.669068110819882	23.485095125466295
68-69	21.900837305287048	26.415826213165044	28.056420347513477	23.62691613403443
70-71	21.928453775127377	26.082497115777997	28.096258501758836	23.89279060733579
72-73	22.466921466931687	26.165448423722363	28.39099955292223	22.976630556423718
74-75	22.598128540487533	26.61496543206161	27.850903150701207	22.93600287674965
76-77	22.33104599029548	26.607070642525038	27.399848773245104	23.662034593934376
78-79	22.007729335572044	26.644522100412633	27.653291020725774	23.694457543289545
80-81	21.74863367113189	27.063385126516675	28.077915053908587	23.110066148442847
82-83	22.47078393238129	26.12275027126679	29.014473326911126	22.391992469440794
84-85	22.837586492609706	26.692854121918785	27.527475378229344	22.942084007242165
86-87	21.500725811324095	27.30566662900788	27.972316974371886	23.22129058529614
88-89	22.398820463948752	26.2034845681709	27.764276516707586	23.633418451172762
90-91	22.01500406872107	26.195971528161323	28.061867020764065	23.727157382353543
92-93	22.352259830346796	26.488104128713086	27.703947591548474	23.455688449391644
94-95	21.25396343743704	26.55815845394439	28.257958488039442	23.929919620579128
96-97	21.658903940673714	26.387885340902223	27.835105729473508	24.118104988950552
98-99	21.006167880170494	27.098928880822072	27.741973232015475	24.15293000699196
100	21.75671648432854	26.50699213786228	26.924551616320848	24.811739761488337
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1174.0
1	762.0
2	519.0
3	657.5
4	411.5
5	281.0
6	651.0
7	1556.5
8	2462.0
9	2623.0
10	1898.0
11	1221.0
12	1179.5
13	1303.0
14	1551.0
15	2127.5
16	2714.5
17	3473.5
18	4743.0
19	6008.0
20	7513.0
21	9299.5
22	11129.5
23	13217.0
24	15510.5
25	17862.5
26	20873.0
27	23907.5
28	28070.0
29	37016.0
30	48695.0
31	58404.0
32	72243.0
33	86176.0
34	94257.5
35	104463.5
36	115560.5
37	134422.5
38	142181.0
39	132714.5
40	124810.0
41	117906.0
42	117296.0
43	122791.0
44	131867.5
45	155494.0
46	178020.5
47	191456.5
48	200852.0
49	195994.5
50	186451.5
51	190931.0
52	194157.5
53	186025.0
54	178215.0
55	154498.0
56	132647.5
57	109957.0
58	85919.5
59	82384.5
60	68306.5
61	45097.5
62	32029.5
63	25662.5
64	17878.5
65	7770.5
66	3576.5
67	2022.0
68	1077.5
69	536.0
70	230.5
71	84.0
72	36.0
73	20.0
74	5.0
75	1.5
76	2.0
77	1.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00714244162345452
3	0.0
4	0.09042600621392421
5	0.00431241758397254
6	0.0
7	8.984203299942794E-5
8	0.0
9	0.0
10-11	0.0
12-13	2.6952609899828377E-4
14-15	0.009366031940190362
16-17	0.0
18-19	1.0107228712435643E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.1230254124928492E-5
60-61	0.0
62-63	0.0
64-65	5.615127062464245E-5
66-67	1.6845381187392737E-4
68-69	0.0
70-71	4.155194026223542E-4
72-73	3.0321686137306926E-4
74-75	2.6952609899828377E-4
76-77	1.0107228712435643E-4
78-79	1.1230254124928492E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06363061987184483
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4452259.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.56688527406575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.88113470439403	48.44155883080801
2	16.090533351752093	21.100123085457163
3	5.338115491651493	10.500108180624796
4	2.183281714905843	5.726039268887877
5	1.0271344630016015	3.367300374833257
6	0.5498820387424334	2.1632431529096703
7	0.3090235696481921	1.4183199056673697
8	0.19555775242256654	1.025769017403566
9	0.11612162621950782	0.6852360009755173
>10	0.29886699066131106	2.728800943469287
>50	0.003941477481338497	0.18094605546022866
>100	0.0050982642159666	0.657796044490204
>500	6.075433491361317E-4	0.28386837909450807
>1k	5.608092436355298E-4	0.5476048996724513
>5k	4.673410363629413E-5	0.2873615773671449
>10k+	9.346820727258826E-5	0.8859242828789606
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15318	0.3440500653713093	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13594	0.3053281491485558	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9378	0.21063464636715878	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.492101649971397E-5
2	0.0	0.0	0.0	0.0	4.492101649971397E-5
3	0.0	0.0	0.0	4.492101649971397E-5	4.492101649971397E-5
4	0.0	0.0	0.0	8.984203299942794E-5	4.492101649971397E-5
5	0.0	0.0	0.0	1.3476304949914188E-4	4.492101649971397E-5
6	0.0	0.0	0.0	1.3476304949914188E-4	4.492101649971397E-5
7	0.0	0.0	0.0	1.3476304949914188E-4	4.492101649971397E-5
8	0.0	0.0	0.0	1.3476304949914188E-4	4.492101649971397E-5
9	0.0	0.0	0.0	1.5722355774899888E-4	4.492101649971397E-5
10-11	0.0	0.0	0.0	2.0214457424871284E-4	4.492101649971397E-5
12-13	0.0	0.0	0.0	2.5829584487335527E-4	6.738152474957096E-5
14-15	0.0	0.0	0.0	2.6952609899828377E-4	8.984203299942794E-5
16-17	0.0	0.0	0.0	2.6952609899828377E-4	8.984203299942794E-5
18-19	0.0	0.0	0.0	2.6952609899828377E-4	8.984203299942794E-5
20-21	0.0	0.0	0.0	3.3690762374785475E-4	1.0107228712435642E-4
22-23	0.0	0.0	0.0	3.930588943724972E-4	1.1230254124928492E-4
24-25	0.0	0.0	0.0	4.941311814968537E-4	1.1230254124928492E-4
26-27	0.0	0.0	0.0	9.321110923690647E-4	1.3476304949914188E-4
28-29	0.0	0.0	0.0	0.0029872475972309784	1.3476304949914188E-4
30-31	0.0	0.0	0.0	0.009882623629937071	1.3476304949914188E-4
32-33	0.0	0.0	0.0	0.021708081223486775	1.3476304949914188E-4
34-35	0.0	0.0	0.0	0.04092304603123942	1.5722355774899888E-4
36-37	0.0	0.0	0.0	0.07432182179877675	1.5722355774899888E-4
38-39	0.0	0.0	0.0	0.12388093325208618	1.5722355774899888E-4
40-41	0.0	0.0	0.0	0.17779738330586786	1.5722355774899888E-4
42-43	0.0	0.0	0.0	0.23443155485788225	1.5722355774899888E-4
44-45	0.0	0.0	0.0	0.3049575507624332	1.5722355774899888E-4
46-47	0.0	0.0	0.0	0.3933284204714955	1.5722355774899888E-4
48-49	0.0	0.0	0.0	0.4971296593482095	1.5722355774899888E-4
50-51	0.0	0.0	0.0	0.6047491846273993	1.5722355774899888E-4
52-53	0.0	0.0	0.0	0.713772491672205	1.5722355774899888E-4
54-55	0.0	0.0	0.0	0.8385406150001606	1.5722355774899888E-4
56-57	0.0	0.0	0.0	0.9898121380629474	1.5722355774899888E-4
58-59	0.0	0.0	0.0	1.1736626283421518	1.5722355774899888E-4
60-61	0.0	0.0	0.0	1.3675080447925425	1.5722355774899888E-4
62-63	0.0	0.0	0.0	1.5663284638202764	1.5722355774899888E-4
64-65	0.0	0.0	0.0	1.7741330861479532	1.5722355774899888E-4
66-67	0.0	0.0	0.0	2.01411238654355	1.5722355774899888E-4
68-69	0.0	0.0	0.0	2.2870974038123117	1.5722355774899888E-4
70-71	0.0	0.0	0.0	2.591414830089624	1.5722355774899888E-4
72-73	0.0	0.0	0.0	2.913734353729197	1.6845381187392737E-4
74-75	0.0	0.0	0.0	3.2271033648312013	1.7968406599885587E-4
76-77	0.0	0.0	0.0	3.591311736356757	1.7968406599885587E-4
78-79	0.0	0.0	0.0	3.9844941635246287	1.7968406599885587E-4
80-81	0.0	0.0	0.0	4.4188579325686135	1.7968406599885587E-4
82-83	0.0	0.0	0.0	4.878175326278188	1.7968406599885587E-4
84-85	0.0	0.0	0.0	5.3223655676814845	1.7968406599885587E-4
86-87	0.0	0.0	0.0	5.791632966545746	1.7968406599885587E-4
88	0.0	0.0	0.0	6.1720353645194495	1.7968406599885587E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9465	0.0	48.21868	1
GGGCACC	4440	0.0	40.140812	94
GTGGTAT	2255	0.0	39.226135	1
GTATCAA	16560	0.0	38.754154	1
GTACTAG	4365	0.0	31.151417	1
CAACGCA	20245	0.0	30.876263	5
ATCAACG	20480	0.0	30.530565	3
CTGTGCG	6185	0.0	30.476753	9
TAGTACT	6490	0.0	30.229513	4
TAGGCAT	5285	0.0	29.435667	5
CTAACGC	2335	0.0	29.21601	3
TATCAAC	22245	0.0	28.78657	2
TCTAACG	2405	0.0	28.758516	2
AACGCAG	22750	0.0	28.404541	6
ACTGTGC	7135	0.0	28.389616	8
TAACGCC	2405	0.0	28.36565	4
CGCCTAC	2415	0.0	27.828215	7
TACTGTG	8040	0.0	26.947056	7
GTACTGT	7445	0.0	26.828148	6
TCAACGC	23940	0.0	26.373528	4
>>END_MODULE