FastQCFastQC Report
Mon 16 Jan 2017
ERR1146646_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146646_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5715197
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC55750.041.67049894
CAACGCA112800.035.8704955
GTATCAA125250.035.139541
ATCAACG121300.033.475763
TACCCCG3650.033.4752465
TATTCTC35700.032.382495
GTACTAG60450.031.3477751
TAGTACT82450.030.4955774
CTGTGCG81950.030.1044629
TATCAAC147900.029.6797352
ACGCAGA135250.029.6373847
CGCAGAG134700.029.4444168
ACTGTGC87200.029.3702858
TAGGCAT75700.029.363555
AACGCAG149450.029.3368746
GTACATG255900.028.5547751
ACGTTAA10600.028.3728038
TACATGG254650.028.3299642
GGTATCA76600.028.237551
TCAAGAC12800.027.9049433
Produced by FastQC (version 0.11.5)