##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146646_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5715197
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63078648032605	33.0	31.0	34.0	28.0	34.0
2	31.682291966488645	33.0	31.0	34.0	28.0	34.0
3	31.768532388297377	33.0	31.0	34.0	28.0	34.0
4	35.17482581965241	37.0	35.0	37.0	32.0	37.0
5	35.30588621879526	37.0	35.0	37.0	32.0	37.0
6	35.38222444475667	37.0	35.0	37.0	32.0	37.0
7	35.26615845438049	37.0	35.0	37.0	32.0	37.0
8	35.271278137918955	37.0	35.0	37.0	32.0	37.0
9	36.77840693855347	39.0	37.0	39.0	32.0	39.0
10-11	36.62328612644498	39.0	37.0	39.0	32.0	39.0
12-13	36.57823054918317	39.0	37.0	39.0	32.0	39.0
14-15	37.78630859093746	40.0	38.0	41.0	32.0	41.0
16-17	37.851260770188674	40.0	38.0	41.0	32.0	41.0
18-19	37.83411446709536	40.0	38.0	41.0	32.0	41.0
20-21	37.904994875942165	40.0	38.0	41.0	32.0	41.0
22-23	37.85854617084941	40.0	38.0	41.0	32.0	41.0
24-25	37.827495710121624	40.0	37.5	41.0	32.0	41.0
26-27	37.8737197510427	40.0	37.0	41.0	32.0	41.0
28-29	37.887574916490195	40.0	37.0	41.0	32.0	41.0
30-31	37.7627442238649	40.0	37.0	41.0	32.0	41.0
32-33	37.76167418200983	40.0	37.0	41.0	32.0	41.0
34-35	37.70037568258802	40.0	37.0	41.0	31.0	41.0
36-37	37.58889466102393	40.0	37.0	41.0	31.0	41.0
38-39	37.477303319553116	40.0	36.0	41.0	31.0	41.0
40-41	37.38213485904335	40.0	36.0	41.0	31.0	41.0
42-43	37.22138668185892	40.0	36.0	41.0	30.0	41.0
44-45	37.05427433210089	39.5	35.5	41.0	30.0	41.0
46-47	37.03675008927951	39.5	35.0	41.0	30.0	41.0
48-49	36.89038243126177	39.0	35.0	41.0	30.0	41.0
50-51	36.69776133701078	39.0	35.0	41.0	30.0	41.0
52-53	36.465180815289486	39.0	35.0	41.0	30.0	41.0
54-55	36.25909141889597	38.5	35.0	40.5	29.5	41.0
56-57	35.99892260931688	38.0	35.0	40.0	29.0	41.0
58-59	35.691545190830695	37.0	34.0	40.0	29.0	41.0
60-61	35.43570326622162	37.0	34.0	40.0	28.0	41.0
62-63	35.125966349016494	36.0	34.0	40.0	28.0	41.0
64-65	34.9649418034059	36.0	34.0	39.5	28.0	41.0
66-67	34.71304987387137	35.0	34.0	39.0	28.0	41.0
68-69	34.58733032299674	35.0	34.0	39.0	28.0	41.0
70-71	34.51665918077715	35.0	34.0	39.0	28.0	41.0
72-73	34.305660854735194	35.0	34.0	38.0	28.0	41.0
74-75	34.0484414622978	35.0	34.0	37.0	28.0	40.0
76-77	33.79612819995531	35.0	33.0	37.0	27.0	39.0
78-79	33.536969854232495	35.0	33.0	36.0	27.0	39.0
80-81	33.359219375990016	35.0	33.0	36.0	27.0	39.0
82-83	33.08698352830183	35.0	33.0	36.0	26.5	37.0
84-85	32.89415867904466	35.0	33.0	35.0	26.0	37.0
86-87	32.71371415543506	35.0	33.0	35.0	26.0	37.0
88-89	32.58139133261723	35.0	33.0	35.0	26.0	36.0
90-91	32.41840080053234	35.0	33.0	35.0	25.5	36.0
92-93	32.25921101232381	35.0	33.0	35.0	25.0	36.0
94-95	32.18283271775233	35.0	33.0	35.0	25.0	36.0
96-97	32.05901458864848	35.0	33.0	35.0	25.0	35.0
98-99	31.963833180203586	35.0	32.5	35.0	25.0	35.0
100	31.052113339225226	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3920450499843433
1101	2	0.41720892586730685
1101	3	0.4969482110233372
1101	4	0.5039218335175235
1101	5	0.4306824411172059
1101	6	0.4330036543896725
1101	7	0.4115872525795865
1101	8	0.4746258407281019
1101	9	0.5770665296831226
1101	10-11	0.6334651554144131
1101	12-13	0.5109222662431705
1101	14-15	0.6875241615521546
1101	16-17	0.6666075221755392
1101	18-19	0.6679057701393276
1101	20-21	0.6658330305184137
1101	22-23	0.7143330197209892
1101	24-25	0.7664659650841088
1101	26-27	0.6857090099467271
1101	28-29	0.6948374020770345
1101	30-31	0.6886774350188247
1101	32-33	0.6812993045854157
1101	34-35	0.7227351302088678
1101	36-37	0.7260229055162526
1101	38-39	0.68212370456569
1101	40-41	0.6509960383853439
1101	42-43	0.6929002495186793
1101	44-45	0.6768003050461644
1101	46-47	0.6756780317356998
1101	48-49	0.6527670627953412
1101	50-51	0.6343267448396119
1101	52-53	0.6343682143569751
1101	54-55	0.5658464075376344
1101	56-57	0.6061335488911155
1101	58-59	0.5855876496139913
1101	60-61	0.619247068511882
1101	62-63	0.6206538783010274
1101	64-65	0.6328219580581944
1101	66-67	0.6431636107603964
1101	68-69	0.6004343037844748
1101	70-71	0.5437854719672188
1101	72-73	0.5841537401524235
1101	74-75	0.5818999325415461
1101	76-77	0.5629048334496858
1101	78-79	0.4917031892153787
1101	80-81	0.49531824652084566
1101	82-83	0.5237478899078667
1101	84-85	0.5213591311301187
1101	86-87	0.5096890928084221
1101	88-89	0.5500909460904495
1101	90-91	0.5613094913331125
1101	92-93	0.5235465004445601
1101	94-95	0.4985070237516851
1101	96-97	0.5091354796849501
1101	98-99	0.517124465799423
1101	100	0.648503342170816
1102	1	0.3697097424925282
1102	2	0.3806674302793098
1102	3	0.40599371108164206
1102	4	0.5058090520655512
1102	5	0.37738142062303126
1102	6	0.376090946987091
1102	7	0.33361024284976537
1102	8	0.38663463716211055
1102	9	0.4505577247426018
1102	10-11	0.6481431714531141
1102	12-13	0.5301510571941677
1102	14-15	0.7009192089279921
1102	16-17	0.6590323904352857
1102	18-19	0.682401568115921
1102	20-21	0.6451779264433597
1102	22-23	0.663259967726141
1102	24-25	0.6631507476463625
1102	26-27	0.589432742008178
1102	28-29	0.6362594378620372
1102	30-31	0.6529780065710753
1102	32-33	0.6090636165222918
1102	34-35	0.6643865311150918
1102	36-37	0.6629611464423064
1102	38-39	0.716741625000509
1102	40-41	0.6324578054142407
1102	42-43	0.6772099583869462
1102	44-45	0.6566793400952591
1102	46-47	0.5878848085292177
1102	48-49	0.5900442979904952
1102	50-51	0.5645981865165055
1102	52-53	0.5000936773967979
1102	54-55	0.519929335866415
1102	56-57	0.4868310969721392
1102	58-59	0.482046003550046
1102	60-61	0.4979487943397345
1102	62-63	0.5566694460750625
1102	64-65	0.5019723609057039
1102	66-67	0.5136387146816332
1102	68-69	0.4508900392681099
1102	70-71	0.4175495357780292
1102	72-73	0.42199552040175803
1102	74-75	0.3628377760308723
1102	76-77	0.41826648883738216
1102	78-79	0.3994535862054249
1102	80-81	0.3885533310487901
1102	82-83	0.3609832917015723
1102	84-85	0.35489793625164623
1102	86-87	0.3418055312066244
1102	88-89	0.4325718944136483
1102	90-91	0.38578423743699375
1102	92-93	0.37495484889993946
1102	94-95	0.40652528657560794
1102	96-97	0.3747221039966959
1102	98-99	0.2877832171877479
1102	100	0.4130719193900312
1103	1	0.27360280436136364
1103	2	0.30401594092864315
1103	3	0.3000799016325857
1103	4	0.399253272208675
1103	5	0.26940858234088694
1103	6	0.32186252257633896
1103	7	0.3368879101203106
1103	8	0.3515958557242058
1103	9	0.43486152043935533
1103	10-11	0.5725963515087642
1103	12-13	0.45843373824326505
1103	14-15	0.5824581820902424
1103	16-17	0.5526918332848112
1103	18-19	0.5385145132388658
1103	20-21	0.47021199707648265
1103	22-23	0.5546221378008269
1103	24-25	0.5511473085362155
1103	26-27	0.4757248408498356
1103	28-29	0.44669457817547453
1103	30-31	0.4606446107344908
1103	32-33	0.4379095107028519
1103	34-35	0.4973896787024472
1103	36-37	0.43771137597873633
1103	38-39	0.5053243645927168
1103	40-41	0.46094616668874977
1103	42-43	0.45112909349465014
1103	44-45	0.41028743962588266
1103	46-47	0.34889410401897436
1103	48-49	0.38402661863598553
1103	50-51	0.37015414497681576
1103	52-53	0.3462588644222677
1103	54-55	0.3588028348540391
1103	56-57	0.4241200590412575
1103	58-59	0.36972637433269284
1103	60-61	0.35688001579479334
1103	62-63	0.3485434907335545
1103	64-65	0.32236660691690133
1103	66-67	0.3062840780775957
1103	68-69	0.3418802831945982
1103	70-71	0.3177367592548208
1103	72-73	0.2713160559957615
1103	74-75	0.30646793936792704
1103	76-77	0.30740107010457507
1103	78-79	0.31941145456149656
1103	80-81	0.27702509158722677
1103	82-83	0.30961802509180814
1103	84-85	0.28289225147764796
1103	86-87	0.3390359612878271
1103	88-89	0.36125380105860216
1103	90-91	0.3508523635943206
1103	92-93	0.32660012370987346
1103	94-95	0.2899913240110763
1103	96-97	0.318939050393233
1103	98-99	0.28762753956265996
1103	100	0.3865187414496418
1104	1	0.19166805508446316
1104	2	0.254247635738146
1104	3	0.25221430262728006
1104	4	0.42424981917032056
1104	5	0.3271409451365628
1104	6	0.28966542060108225
1104	7	0.2553702400266218
1104	8	0.2856095886793639
1104	9	0.3763027442667024
1104	10-11	0.5007932139874711
1104	12-13	0.39502987305491644
1104	14-15	0.5607642673238402
1104	16-17	0.5427885453512289
1104	18-19	0.4882494935035595
1104	20-21	0.44594268928560155
1104	22-23	0.44278370061147854
1104	24-25	0.4828211344870752
1104	26-27	0.45988363822382894
1104	28-29	0.4017437867709788
1104	30-31	0.3805137998567858
1104	32-33	0.42249030264443377
1104	34-35	0.42810373942862157
1104	36-37	0.4699266211668487
1104	38-39	0.41319681047541224
1104	40-41	0.4636300585799873
1104	42-43	0.533581369099835
1104	44-45	0.468318620983986
1104	46-47	0.44765089365245814
1104	48-49	0.42628394043459394
1104	50-51	0.3369341752838011
1104	52-53	0.3534442668624962
1104	54-55	0.41717104594521004
1104	56-57	0.43798503176063974
1104	58-59	0.4248940567278865
1104	60-61	0.4248757395177378
1104	62-63	0.4188355808172588
1104	64-65	0.4172282113173793
1104	66-67	0.4578570102812165
1104	68-69	0.4232118588294185
1104	70-71	0.41378100820448793
1104	72-73	0.38845449680761845
1104	74-75	0.3639931812086701
1104	76-77	0.3283356078282438
1104	78-79	0.29201143800408147
1104	80-81	0.3043395375139042
1104	82-83	0.32232916308902304
1104	84-85	0.3405436216214852
1104	86-87	0.30675979140564635
1104	88-89	0.298292949265587
1104	90-91	0.3524300869557422
1104	92-93	0.31072145373896376
1104	94-95	0.36740346722816497
1104	96-97	0.33581127214667106
1104	98-99	0.324448791310612
1104	100	0.3579787314630529
1105	1	0.11806388931992373
1105	2	0.20828325568707484
1105	3	0.18087933917136212
1105	4	0.2542174677145823
1105	5	0.14524904304033015
1105	6	0.18292660646940107
1105	7	0.18452287705173376
1105	8	0.1954188865214448
1105	9	0.3506074588733057
1105	10-11	0.3590838691245324
1105	12-13	0.24997761079587377
1105	14-15	0.43425191551542497
1105	16-17	0.3341905055227983
1105	18-19	0.33678700136140094
1105	20-21	0.34170424258210375
1105	22-23	0.3501234473530346
1105	24-25	0.31282732832718096
1105	26-27	0.2964994863913404
1105	28-29	0.34733949432815336
1105	30-31	0.39046740258341117
1105	32-33	0.33271582711039116
1105	34-35	0.386678887432673
1105	36-37	0.3293509388898741
1105	38-39	0.3248549244379504
1105	40-41	0.33597060559326763
1105	42-43	0.3190333391796969
1105	44-45	0.28658407840487854
1105	46-47	0.3379641287532351
1105	48-49	0.2677305217100283
1105	50-51	0.31937183354722265
1105	52-53	0.3082998826973693
1105	54-55	0.2920026813586176
1105	56-57	0.29316985755631464
1105	58-59	0.3669735388201758
1105	60-61	0.402400312410478
1105	62-63	0.34610837208328604
1105	64-65	0.3125888466087048
1105	66-67	0.29862354190069595
1105	68-69	0.2946279444547244
1105	70-71	0.2880233151258622
1105	72-73	0.3040489095670864
1105	74-75	0.2882829960426463
1105	76-77	0.24142526606350856
1105	78-79	0.3408902647765757
1105	80-81	0.21383574459563448
1105	82-83	0.2656255985983904
1105	84-85	0.19783220572215043
1105	86-87	0.21015725953218123
1105	88-89	0.20811744907086904
1105	90-91	0.22840192649213265
1105	92-93	0.2162847963307044
1105	94-95	0.2087679308829209
1105	96-97	0.09978618876518652
1105	98-99	0.19699726676629936
1105	100	0.3294416744024318
1106	1	0.08789700150177993
1106	2	0.18039292333359214
1106	3	0.16918055661349385
1106	4	0.2738395400669944
1106	5	0.13908283107153352
1106	6	0.1733266753590641
1106	7	0.17377354539888756
1106	8	0.1515326484058903
1106	9	0.21572926179928942
1106	10-11	0.33566291675104054
1106	12-13	0.2526808450872764
1106	14-15	0.3560779351933405
1106	16-17	0.2938047886462627
1106	18-19	0.32147020676610794
1106	20-21	0.33054030084083763
1106	22-23	0.30484118239660773
1106	24-25	0.40177079835691387
1106	26-27	0.38245408445016693
1106	28-29	0.3639477417777357
1106	30-31	0.44139643448794175
1106	32-33	0.31477727851643067
1106	34-35	0.3573298034172936
1106	36-37	0.4363396843021192
1106	38-39	0.4250384344967486
1106	40-41	0.46507192167287315
1106	42-43	0.3457820667432614
1106	44-45	0.36435982298079495
1106	46-47	0.38537913547910563
1106	48-49	0.3098059598052245
1106	50-51	0.28286530272887944
1106	52-53	0.28116061728559316
1106	54-55	0.370319301120297
1106	56-57	0.33016748816941544
1106	58-59	0.40958016449227586
1106	60-61	0.36691910245748716
1106	62-63	0.4203795507619361
1106	64-65	0.35084237622538694
1106	66-67	0.30280911082101625
1106	68-69	0.34242794393283305
1106	70-71	0.32020581271537907
1106	72-73	0.28188842274679615
1106	74-75	0.29730062683841396
1106	76-77	0.25413306038088734
1106	78-79	0.2897682635318688
1106	80-81	0.291081149668166
1106	82-83	0.24567646343765404
1106	84-85	0.22961897916779606
1106	86-87	0.2740995879245034
1106	88-89	0.32128620395544516
1106	90-91	0.2830546208216518
1106	92-93	0.3103168378523833
1106	94-95	0.2692184470987442
1106	96-97	0.28964212064791894
1106	98-99	0.287221588731537
1106	100	0.28778567389097987
1107	1	0.09464261408402308
1107	2	0.13920748866487287
1107	3	0.1372907983499161
1107	4	0.177855554085518
1107	5	0.11015864847249901
1107	6	0.15930280044094047
1107	7	0.14211626160740565
1107	8	0.11387298482837593
1107	9	0.22193679555336843
1107	10-11	0.23142564918173036
1107	12-13	0.09763069244771572
1107	14-15	0.21895807673956824
1107	16-17	0.18218146534212565
1107	18-19	0.08512585533036088
1107	20-21	0.12865249654371524
1107	22-23	0.2307479379166253
1107	24-25	0.28332933303719443
1107	26-27	0.21534429355209994
1107	28-29	0.2306619232709508
1107	30-31	0.33413358371844026
1107	32-33	0.25071358621535467
1107	34-35	0.2502705554516993
1107	36-37	0.26001884301073375
1107	38-39	0.2723724086443511
1107	40-41	0.26122500956533656
1107	42-43	0.2462364702731108
1107	44-45	0.2719029716138479
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2216	78-79	-0.8708454943471935
2216	80-81	-0.9514546032018103
2216	82-83	-0.9130628288147591
2216	84-85	-0.9421369814218714
2216	86-87	-0.9368993521632234
2216	88-89	-0.9731163302327843
2216	90-91	-1.0566834715007332
2216	92-93	-0.9875670269669072
2216	94-95	-1.0612583589582023
2216	96-97	-1.0770544201798167
2216	98-99	-1.0904412213717016
2216	100	-0.7696460468895907
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	6.0
3	34.0
4	48.0
5	44.0
6	67.0
7	254.0
8	631.0
9	1004.0
10	1383.0
11	1917.0
12	3032.0
13	4568.0
14	7086.0
15	11677.0
16	15135.0
17	17745.0
18	20063.0
19	22506.0
20	25769.0
21	29372.0
22	34140.0
23	40479.0
24	47768.0
25	55731.0
26	60170.0
27	66384.0
28	77066.0
29	92553.0
30	107385.0
31	126189.0
32	158603.0
33	207923.0
34	309920.0
35	456203.0
36	732425.0
37	1338299.0
38	1143956.0
39	497209.0
40	452.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16236307462176	21.756391485034698	11.663581521623998	24.417663918719544
2	15.636682426970067	20.90264192147379	40.472231651962105	22.988443999594036
3	17.67176468817796	29.19526363809122	29.333886986798756	23.799084686932062
4	11.562142805646374	17.540714469201752	42.112396223337946	28.784746501813924
5	12.31411590509583	39.61883721149973	35.78761172818271	12.279435155221728
6	27.50541637401974	40.45356861271185	19.35940331872986	12.681611694538553
7	25.03430796593962	32.66354571931246	25.8315055873473	16.470640727400617
8	25.41597063372345	34.98909123104351	21.042996944774806	18.551941190458233
9	26.85436708717902	14.683952588645205	22.175906897623886	36.28577342655189
10-11	25.066771790297604	26.228319672772955	28.25468789191163	20.450220645017815
12-13	25.57863200343996	24.617849036316862	27.831403459119635	21.972115501123543
14-15	22.94433307454924	25.52611120997048	25.518114973975486	26.01144074150479
16-17	21.706829666804254	27.61536885435934	26.94889687426566	23.72890460457075
18-19	20.943182938617667	28.854865408871277	28.50395325160664	21.69799840090441
20-21	22.859024825459247	27.792883850109735	27.08207024113666	22.26602108329436
22-23	23.582125879005577	27.518751564926948	26.549787292416976	22.349335263650495
24-25	22.979323715283442	27.547038580537865	27.62662567600257	21.847012028176124
26-27	22.679209349724374	27.97291754739114	26.650964690764223	22.696908412120262
28-29	21.763324730481752	28.72669135098016	26.915805528484814	22.594178390053273
30-31	22.64064955066178	28.154284992863836	26.976811248551925	22.22825420792246
32-33	23.322608066627772	28.489949174554592	25.76928474463215	22.418158014185487
34-35	23.0629760156444	27.91645239200513	27.27716295507083	21.743408637279643
36-37	22.756877780072024	27.704023601958433	26.99819590605413	22.540902711915418
38-39	23.05739979325873	27.57842804017063	27.597448586998226	21.76672357957242
40-41	22.352888887800113	26.713653355161725	28.33387081900743	22.59958693803073
42-43	22.45711280779643	27.117121452608405	27.33298800751084	23.092777732084325
44-45	22.340368841108724	28.505982539816294	27.243969792896905	21.909678826178077
46-47	22.333739019543728	27.81782673701672	26.84332231718373	23.005111926255818
48-49	23.25914156260279	26.916606645941542	27.330379943221892	22.49387184823377
50-51	22.909347908490396	27.00499702670436	26.807690248045308	23.277964816759937
52-53	21.92391218457065	27.997816506569777	26.801480526156112	23.276790782703465
54-55	22.158187251581687	27.896457777111983	26.670431588099163	23.274923383207167
56-57	23.536998924904392	26.891416218622666	26.527168191869787	23.04441666460316
58-59	22.190826943589123	27.237906736422286	27.828703229741166	22.742563090247426
60-61	22.31633946712705	28.225662289335414	26.622778729916813	22.83521951362072
62-63	22.94875894801174	27.324212555280482	26.101013936652695	23.626014560055086
64-65	21.672951005778618	27.527417698781324	26.854903731274398	23.94472756416566
66-67	22.845976179161934	26.762896974958483	26.772302953713574	23.61882389216601
68-69	21.890141544252582	27.19627277796136	27.028046642419636	23.88553903536642
70-71	22.026959219947358	26.830648974997562	27.176220100905685	23.9661717041494
72-73	22.819308740753545	27.025592855271764	27.71484326810632	22.440255135868373
74-75	22.948070887925976	27.463261632751767	27.031157747229113	22.55750973209314
76-77	22.44183395218344	27.293354549051372	26.947006727118517	23.31780477164667
78-79	22.234614572248685	27.38320290304726	26.876189836219737	23.50599268848432
80-81	21.898867944074237	27.693005359736127	27.658427545918407	22.749699150271223
82-83	22.87674640242435	26.26181886838609	28.790544222859175	22.070890506330386
84-85	23.278032169257173	27.16089047079704	26.961638289876795	22.599439070068993
86-87	21.620274694603925	27.918398308925564	27.623256473206865	22.83807052326365
88-89	22.98041621557019	26.42633249684721	27.293846358713086	23.299404928869517
90-91	22.55024731012506	26.41454129287241	27.633496281817173	23.401715115185358
92-93	23.038323858133253	26.81605279537505	27.352804841792533	22.79281850469916
94-95	21.629361113548732	26.93836479792287	27.963516900516648	23.468757188011747
96-97	22.097284917629366	26.7747227167325	27.453981087508094	23.674011278130035
98-99	21.35130157466364	27.89610150991848	27.1739741938024	23.57862272161548
100	22.381708600334665	26.881787020919525	26.238720681315236	24.497783697430577
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	524.0
1	385.5
2	305.5
3	399.5
4	413.5
5	381.5
6	372.5
7	387.0
8	440.5
9	445.5
10	394.0
11	390.5
12	382.0
13	384.0
14	428.5
15	540.0
16	636.0
17	722.0
18	873.5
19	1049.5
20	1309.5
21	1797.5
22	2540.5
23	3594.5
24	5006.5
25	6556.0
26	9196.0
27	12218.5
28	17180.5
29	30463.5
30	50318.0
31	69274.0
32	93942.5
33	116635.0
34	136420.5
35	158217.0
36	176565.0
37	210647.0
38	218067.0
39	193119.5
40	172745.0
41	153055.0
42	147624.0
43	157875.5
44	174254.0
45	209549.0
46	243729.5
47	265942.5
48	277325.5
49	264931.0
50	247022.5
51	249197.0
52	250826.0
53	238179.0
54	223798.5
55	191670.5
56	168233.5
57	133810.5
58	97196.5
59	93649.5
60	76128.0
61	49295.5
62	35587.5
63	29547.5
64	21237.5
65	8630.5
66	3399.0
67	2143.0
68	1558.5
69	1091.0
70	810.0
71	638.0
72	491.0
73	366.0
74	278.0
75	194.0
76	111.0
77	56.5
78	39.5
79	36.5
80	28.0
81	28.0
82	29.5
83	17.5
84	11.5
85	15.0
86	14.0
87	12.0
88	12.5
89	11.0
90	10.0
91	8.5
92	9.5
93	10.0
94	6.5
95	5.5
96	4.5
97	4.5
98	5.5
99	9.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059805462523864004
2	0.007296336416749939
3	0.007541297351604853
4	0.0091160462185293
5	0.0036919112324562044
6	0.002082167946266769
7	9.098549008896805E-4
8	0.0012248046742745701
9	4.5492745044484027E-4
10-11	3.7619000709861796E-4
12-13	2.537095396711609E-4
14-15	1.3997767705995086E-4
16-17	4.899218697098281E-4
18-19	0.001600994681373188
20-21	9.798437394196561E-4
22-23	0.0012947935128045454
24-25	0.0013035421176207924
26-27	0.0035869279746612408
28-29	0.004050604029922329
30-31	0.005117933817504453
32-33	0.006001542903945394
34-35	0.0031494977338488946
36-37	0.007296336416749939
38-39	0.005677844525744257
40-41	0.005529118243868059
42-43	0.013770303980772667
44-45	0.01943065129688443
46-47	0.017680930333635044
48-49	0.02097040574454389
50-51	0.017479712422861365
52-53	0.024802294654060045
54-55	0.01828458406595608
56-57	0.014373957713093705
58-59	0.016832315666459093
60-61	0.015231320985085903
62-63	0.010314605078355129
64-65	0.010402091126517599
66-67	0.013061666990656664
68-69	0.004732995205589588
70-71	0.007952481777968458
72-73	0.00766377781903231
74-75	0.01048082856986382
76-77	0.009903420651991525
78-79	0.011373186261121009
80-81	0.009623465297871623
82-83	0.005975297089496653
84-85	0.01013963298203019
86-87	0.008521141091024509
88-89	0.0073138336263824325
90-91	0.012458013258335628
92-93	0.007943733173152211
94-95	0.006762671522958876
96-97	0.008897331098123126
98-99	0.008477398066943274
100	0.26531018965750436
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5715197.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.42587237640237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.00524574477973	47.76431890905851
2	15.808372744226789	20.685531552847593
3	5.660126990334057	11.109562383114795
4	2.4590294259804653	6.435365815760635
5	1.230028318293764	4.023783788602431
6	0.7034904306883913	2.761588508174342
7	0.37926044340860365	1.7369411757508353
8	0.24099693540662495	1.2613947791214342
9	0.1569364473991167	0.9240933580866532
>10	0.3537732300336875	3.0769050523172314
>50	0.001956284580160505	0.08835958270454417
>100	7.063432600699306E-4	0.09443738693490904
>500	7.666160839730242E-5	0.03771770752608339
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	8.748604816246929E-6	0.0
20-21	0.0	0.0	0.0	8.748604816246929E-5	0.0
22-23	0.0	0.0	0.0	3.6744140228237097E-4	0.0
24-25	0.0	0.0	0.0	6.998883852997543E-4	0.0
26-27	0.0	0.0	0.0	0.001461017004313237	0.0
28-29	0.0	0.0	0.0	0.005467878010154331	0.0
30-31	0.0	0.0	0.0	0.019010718265704576	0.0
32-33	0.0	0.0	0.0	0.04470537061102181	0.0
34-35	0.0	0.0	0.0	0.08132703037183145	0.0
36-37	0.0	0.0	0.0	0.13887535285310376	0.0
38-39	0.0	0.0	0.0	0.2306569659803503	0.0
40-41	0.0	0.0	0.0	0.3433914876425082	0.0
42-43	0.0	0.0	0.0	0.45849688120986903	0.0
44-45	0.0	0.0	0.0	0.5847567459179448	0.0
46-47	0.0	0.0	0.0	0.7380760453226722	0.0
48-49	0.0	0.0	0.0	0.9201607573632196	0.0
50-51	0.0	0.0	0.0	1.1234608360831657	0.0
52-53	0.0	0.0	0.0	1.3267171717790305	0.0
54-55	0.0	0.0	0.0	1.528967417921027	0.0
56-57	0.0	0.0	0.0	1.7742083081300608	0.0
58-59	0.0	0.0	0.0	2.075020336131895	0.0
60-61	0.0	0.0	0.0	2.398552490841523	0.0
62-63	0.0	0.0	0.0	2.742215185233335	0.0
64-65	0.0	0.0	0.0	3.0900684613321294	0.0
66-67	0.0	0.0	0.0	3.4719363129564913	0.0
68-69	0.0	0.0	0.0	3.8899359024719535	0.0
70-71	0.0	0.0	0.0	4.359001098299848	0.0
72-73	0.0	0.0	0.0	4.8736727710348395	0.0
74-75	0.0	0.0	0.0	5.367890205709445	0.0
76-77	0.0	0.0	0.0	5.913890632291416	0.0
78-79	0.0	0.0	0.0	6.491683488775628	0.0
80-81	0.0	1.7497209632493858E-5	0.0	7.13402355159411	0.0
82-83	0.0	3.4994419264987715E-5	0.0	7.831208618005643	0.0
84-85	0.0	3.4994419264987715E-5	0.0	8.512033793410795	0.0
86-87	0.0	3.4994419264987715E-5	0.0	9.214529262945792	0.0
88	0.0	3.4994419264987715E-5	0.0	9.76067841580964	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5575	0.0	41.670498	94
CAACGCA	11280	0.0	35.870495	5
GTATCAA	12525	0.0	35.13954	1
ATCAACG	12130	0.0	33.47576	3
TACCCCG	365	0.0	33.475246	5
TATTCTC	3570	0.0	32.38249	5
GTACTAG	6045	0.0	31.347775	1
TAGTACT	8245	0.0	30.495577	4
CTGTGCG	8195	0.0	30.104462	9
TATCAAC	14790	0.0	29.679735	2
ACGCAGA	13525	0.0	29.637384	7
CGCAGAG	13470	0.0	29.444416	8
ACTGTGC	8720	0.0	29.370285	8
TAGGCAT	7570	0.0	29.36355	5
AACGCAG	14945	0.0	29.336874	6
GTACATG	25590	0.0	28.554775	1
ACGTTAA	1060	0.0	28.372803	8
TACATGG	25465	0.0	28.329964	2
GGTATCA	7660	0.0	28.23755	1
TCAAGAC	1280	0.0	27.904943	3
>>END_MODULE