FastQCFastQC Report
Mon 16 Jan 2017
ERR1146646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5715197
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171330.2997796926335173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112360.196598647430701No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83570.14622418089875117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA209850.044.7688141
GGTATCA109250.044.2668041
GGGCACC58450.038.94104894
GTGGTAT17850.036.897441
CAACGCA248550.036.4570545
ATCAACG253250.035.8303643
TATCAAC273650.034.210872
AACGCAG293550.032.1477786
CTGTGCG76600.031.7875989
TAGTACT78350.031.400114
TACCCCG3150.031.3326455
ACTGTGC81350.030.8501198
ACGCAGA296700.030.475927
GTACTAG58100.030.4451541
CGCAGAG298600.030.2193098
TATTCTC39050.029.7279015
GTACTGT83900.029.2400326
TCAACGC323200.028.3521524
TAGGCAT77300.028.211545
GCAGAGT320950.027.8567129
Produced by FastQC (version 0.11.5)