##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146646_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5715197 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14027880403773 33.0 31.0 34.0 30.0 34.0 2 32.23194808507913 33.0 31.0 34.0 30.0 34.0 3 32.38341303020701 34.0 31.0 34.0 30.0 34.0 4 35.98042149728172 37.0 35.0 37.0 35.0 37.0 5 35.88645430769928 37.0 35.0 37.0 35.0 37.0 6 35.91943549802395 37.0 35.0 37.0 35.0 37.0 7 35.846498379670905 37.0 35.0 37.0 35.0 37.0 8 35.85213790530755 37.0 35.0 37.0 35.0 37.0 9 37.56932508188257 39.0 37.0 39.0 35.0 39.0 10-11 37.498299971112104 39.0 37.0 39.0 34.5 39.0 12-13 37.45567983045904 39.0 37.0 39.0 34.0 39.0 14-15 38.662047869915945 40.0 38.0 41.0 34.0 41.0 16-17 38.68110906763144 40.0 38.0 41.0 34.0 41.0 18-19 38.73787797690963 40.0 38.0 41.0 34.0 41.0 20-21 38.73486478243882 40.0 38.0 41.0 34.0 41.0 22-23 38.694787598747695 40.0 38.0 41.0 34.0 41.0 24-25 38.608622939856666 40.0 38.0 41.0 34.0 41.0 26-27 38.4506157880472 40.0 38.0 41.0 34.0 41.0 28-29 38.40548042350946 40.0 38.0 41.0 34.0 41.0 30-31 38.25970189654005 40.0 37.5 41.0 34.0 41.0 32-33 38.09454468498636 40.0 37.0 41.0 33.0 41.0 34-35 37.983133652260804 40.0 37.0 41.0 33.0 41.0 36-37 37.95300931883888 40.0 37.0 41.0 33.0 41.0 38-39 37.84189188929095 40.0 37.0 41.0 33.0 41.0 40-41 37.710855636297396 40.0 37.0 41.0 33.0 41.0 42-43 37.56364889959174 40.0 36.0 41.0 32.5 41.0 44-45 37.328968975172685 40.0 36.0 41.0 31.5 41.0 46-47 37.06019398806375 40.0 35.5 41.0 31.0 41.0 48-49 36.76439517307978 39.0 35.0 41.0 30.5 41.0 50-51 36.54202357329065 39.0 35.0 41.0 30.0 41.0 52-53 36.34640118267139 39.0 35.0 41.0 30.0 41.0 54-55 36.1609929631472 38.5 35.0 41.0 30.0 41.0 56-57 35.9157840928318 38.0 35.0 40.0 29.5 41.0 58-59 35.62956762470305 37.5 35.0 40.0 29.0 41.0 60-61 35.4377182448829 37.0 35.0 40.0 29.0 41.0 62-63 35.21288102579841 36.5 34.5 40.0 29.0 41.0 64-65 34.970528312497365 36.0 34.0 39.5 29.0 41.0 66-67 34.72928002306832 35.5 34.0 39.0 28.5 41.0 68-69 34.49868552212636 35.0 34.0 39.0 28.0 41.0 70-71 34.30565464322577 35.0 34.0 38.5 28.0 41.0 72-73 34.086357390655124 35.0 34.0 37.5 28.0 40.5 74-75 33.83327355120042 35.0 34.0 37.0 28.0 40.0 76-77 33.60681416581091 35.0 34.0 36.5 27.5 39.0 78-79 33.392456550491616 35.0 33.0 36.0 27.0 39.0 80-81 33.27433700010691 35.0 33.0 36.0 27.0 38.5 82-83 33.11041106719506 35.0 33.0 36.0 27.0 37.0 84-85 32.972057306161105 35.0 33.0 35.0 27.0 37.0 86-87 32.83304958691713 35.0 33.0 35.0 27.0 36.5 88-89 32.7338845887552 35.0 33.0 35.0 27.0 36.0 90-91 32.663043548630085 35.0 33.0 35.0 27.0 36.0 92-93 32.5927980260348 35.0 33.0 35.0 27.0 36.0 94-95 32.554760142126334 35.0 33.0 35.0 27.0 36.0 96-97 32.46577055874015 35.0 33.0 35.0 27.0 35.0 98-99 32.3944875390997 35.0 33.0 35.0 27.0 35.0 100 31.199496185345843 34.0 31.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4308142701187023 1101 2 0.48182766459826354 1101 3 0.3944804498777046 1101 4 0.296187825662237 1101 5 0.2863883307355337 1101 6 0.291399916773436 1101 7 0.314748004933449 1101 8 0.33817083374025714 1101 9 0.4158087590879447 1101 10-11 0.43566223714513796 1101 12-13 0.445419234106069 1101 14-15 0.6058674543403981 1101 16-17 0.6110539620279738 1101 18-19 0.5697596946015793 1101 20-21 0.5863687674628153 1101 22-23 0.5606754229712436 1101 24-25 0.5240926238435861 1101 26-27 0.5974914976970211 1101 28-29 0.5911302603398596 1101 30-31 0.5949378335479523 1101 32-33 0.6558045337074176 1101 34-35 0.6689599207382741 1101 36-37 0.5903695614403475 1101 38-39 0.5675234206097102 1101 40-41 0.5297312829985046 1101 42-43 0.502072328530268 1101 44-45 0.5438223949008503 1101 46-47 0.5902515734165306 1101 48-49 0.5230987167344736 1101 50-51 0.5251359242147373 1101 52-53 0.6354948739442747 1101 54-55 0.6089964491791804 1101 56-57 0.5783063675310558 1101 58-59 0.5058775895989953 1101 60-61 0.5123682865158798 1101 62-63 0.5504008695960252 1101 64-65 0.5584933510574999 1101 66-67 0.5082855463777634 1101 68-69 0.5043015062701386 1101 70-71 0.4878824551367771 1101 72-73 0.5264855820568286 1101 74-75 0.5205196656453595 1101 76-77 0.5155936317893719 1101 78-79 0.544839906128125 1101 80-81 0.525380579849589 1101 82-83 0.4342747650303238 1101 84-85 0.4163796156698609 1101 86-87 0.4091209986422868 1101 88-89 0.4824110539498321 1101 90-91 0.4698422664313284 1101 92-93 0.4840653795743961 1101 94-95 0.44153219910592156 1101 96-97 0.4344818011359095 1101 98-99 0.5131059755729623 1101 100 0.8579764225119391 1102 1 0.28963316675943673 1102 2 0.36202803610372314 1102 3 0.2802593850937285 1102 4 0.2311690247139495 1102 5 0.23379595330176528 1102 6 0.1919068505917494 1102 7 0.278450253970135 1102 8 0.28701196306652577 1102 9 0.3083440100405781 1102 10-11 0.3113386506188647 1102 12-13 0.3447525409746959 1102 14-15 0.43563857319705335 1102 16-17 0.39929012210547654 1102 18-19 0.4177810135555049 1102 20-21 0.41962202563222917 1102 22-23 0.42744835243344426 1102 24-25 0.4603857603147077 1102 26-27 0.47929129570034235 1102 28-29 0.44418785471806643 1102 30-31 0.49276336674514454 1102 32-33 0.5014765012655218 1102 34-35 0.4633138409474924 1102 36-37 0.5024098436266442 1102 38-39 0.4825601939951767 1102 40-41 0.43039427832132304 1102 42-43 0.4547954965205463 1102 44-45 0.46393880750760985 1102 46-47 0.5405722767397165 1102 48-49 0.5234896201564894 1102 50-51 0.5245115849746682 1102 52-53 0.5558575248659494 1102 54-55 0.5148850073282674 1102 56-57 0.459532430338335 1102 58-59 0.458431846976886 1102 60-61 0.4531975698036703 1102 62-63 0.48296413688644435 1102 64-65 0.4259839816573745 1102 66-67 0.45361004422740336 1102 68-69 0.44614150318138 1102 70-71 0.44105083617015595 1102 72-73 0.45817823294974147 1102 74-75 0.4709085142976974 1102 76-77 0.4217806326094973 1102 78-79 0.4543233634455106 1102 80-81 0.4469411809112813 1102 82-83 0.37553485078375104 1102 84-85 0.34441593405396986 1102 86-87 0.3678489416220785 1102 88-89 0.39089493768047134 1102 90-91 0.36466156672359773 1102 92-93 0.43351968479851166 1102 94-95 0.35373186771701626 1102 96-97 0.3376421378819785 1102 98-99 0.3743799063566726 1102 100 0.6630397478719381 1103 1 0.2567508746936724 1103 2 0.3501224869442794 1103 3 0.24917567574688348 1103 4 0.20461008947670223 1103 5 0.19209318027269973 1103 6 0.21171692977366519 1103 7 0.1943413634473643 1103 8 0.20711332648030378 1103 9 0.2366617835634699 1103 10-11 0.331632525634312 1103 12-13 0.33271526581741284 1103 14-15 0.43021427711803994 1103 16-17 0.39170621944388273 1103 18-19 0.39159076797499637 1103 20-21 0.35371377149751737 1103 22-23 0.3579533262130923 1103 24-25 0.41156909952461973 1103 26-27 0.4401836003733166 1103 28-29 0.47698408950954274 1103 30-31 0.35459077093744185 1103 32-33 0.44700790851268124 1103 34-35 0.4554062334749034 1103 36-37 0.4330505194378702 1103 38-39 0.3758976258240878 1103 40-41 0.47855211827261 1103 42-43 0.4418428111630206 1103 44-45 0.4964645447716123 1103 46-47 0.49755777837124526 1103 48-49 0.5364880617103083 1103 50-51 0.48508389845216726 1103 52-53 0.5052365002038783 1103 54-55 0.39546113620775003 1103 56-57 0.44095547030876503 1103 58-59 0.49810910118523566 1103 60-61 0.5123141619038023 1103 62-63 0.5297665787437111 1103 64-65 0.5043292295734076 1103 66-67 0.4810770180983468 1103 68-69 0.43982052402105154 1103 70-71 0.45082710793238334 1103 72-73 0.47158672286326464 1103 74-75 0.3939758000805327 1103 76-77 0.38445360399333595 1103 78-79 0.44418593263446127 1103 80-81 0.4048087179158344 1103 82-83 0.3385913026698404 1103 84-85 0.3515853033898182 1103 86-87 0.3904402007413097 1103 88-89 0.3726737929103834 1103 90-91 0.3686263562411227 1103 92-93 0.39458827885548686 1103 94-95 0.3921777045154684 1103 96-97 0.41454825243341986 1103 98-99 0.43022753074807696 1103 100 0.6230330395078099 1104 1 0.19573966678910892 1104 2 0.22729054336878818 1104 3 0.1928465056597659 1104 4 0.16154774887586854 1104 5 0.16138468060229627 1104 6 0.1647409003165734 1104 7 0.21686863907632414 1104 8 0.18379491168364126 1104 9 0.1927027572261224 1104 10-11 0.2528556539333948 1104 12-13 0.27394971376361354 1104 14-15 0.29595201742090893 1104 16-17 0.2892316968549906 1104 18-19 0.28377149719595707 1104 20-21 0.3457377436660991 1104 22-23 0.3581641432916882 1104 24-25 0.3164029943466815 1104 26-27 0.3856767419474494 1104 28-29 0.38252587636540625 1104 30-31 0.35183211305913886 1104 32-33 0.3984807033708009 1104 34-35 0.46917016092131547 1104 36-37 0.3599636077430475 1104 38-39 0.4429179103041534 1104 40-41 0.4532649808412472 1104 42-43 0.4941626302719655 1104 44-45 0.4966524029409527 1104 46-47 0.47761255952499226 1104 48-49 0.38122688885757583 1104 50-51 0.4619845364951374 1104 52-53 0.4719949672333641 1104 54-55 0.4686412638347406 1104 56-57 0.41391074172187103 1104 58-59 0.37538190667468996 1104 60-61 0.380754857507533 1104 62-63 0.3973485543957267 1104 64-65 0.41414750819629376 1104 66-67 0.37338833090840495 1104 68-69 0.4449536959702769 1104 70-71 0.40259053160173863 1104 72-73 0.36228596596768625 1104 74-75 0.38918695042372775 1104 76-77 0.28309677539873235 1104 78-79 0.34066809862304837 1104 80-81 0.27832447504314217 1104 82-83 0.2830210571953913 1104 84-85 0.3283218013350364 1104 86-87 0.4025777724273212 1104 88-89 0.3914702074434473 1104 90-91 0.32241583847185495 1104 92-93 0.3040443143539804 1104 94-95 0.2910714148264262 1104 96-97 0.30910482234231296 1104 98-99 0.27962967737695266 1104 100 0.43488901104649 1105 1 0.18874676371684274 1105 2 0.1594016758346939 1105 3 0.15738363894351437 1105 4 0.14954525143080133 1105 5 0.11036903943176668 1105 6 0.13673588352651223 1105 7 0.19725244669597686 1105 8 0.16631920976071513 1105 9 0.2445684853528718 1105 10-11 0.24018050161333804 1105 12-13 0.20927308220295515 1105 14-15 0.30066663113639436 1105 16-17 0.35667324413327606 1105 18-19 0.25972052667717804 1105 20-21 0.30341655178099103 1105 22-23 0.2648875350388167 1105 24-25 0.30962771114612053 1105 26-27 0.2986253376252179 1105 28-29 0.33635994587051954 1105 30-31 0.331039439466835 1105 32-33 0.32316526441197624 1105 34-35 0.30456400731166156 1105 36-37 0.3332003951003628 1105 38-39 0.2968628304202454 1105 40-41 0.3401228125506819 1105 42-43 0.3259255894300921 1105 44-45 0.3566646839656684 1105 46-47 0.33902410375932135 1105 48-49 0.3685579225562279 1105 50-51 0.30529515007496144 1105 52-53 0.3395422598796216 1105 54-55 0.27781224987118947 1105 56-57 0.1804979287137769 1105 58-59 0.2060917068143837 1105 60-61 0.24639733989852886 1105 62-63 0.30156772489613104 1105 64-65 0.26302186254836357 1105 66-67 0.2784718375391435 1105 68-69 0.23776345890141926 1105 70-71 0.2700738167303456 1105 72-73 0.20911564204855182 1105 74-75 0.2851717763331578 1105 76-77 0.3042838041280902 1105 78-79 0.24951507948684792 1105 80-81 0.14231856120926523 1105 82-83 0.22018035777141165 1105 84-85 0.2686268701800856 1105 86-87 0.2195564739649285 1105 88-89 0.24446283619778342 1105 90-91 0.21109226724486518 1105 92-93 0.19982486654929232 1105 94-95 0.21004417593737656 1105 96-97 0.18791844065628283 1105 98-99 0.1707040416408816 1105 100 0.3326080268608713 1106 1 0.11149007274308076 1106 2 0.16637186148450667 1106 3 0.09637890669336713 1106 4 0.13033912651349056 1106 5 0.07640676174118965 1106 6 0.12374404982197973 1106 7 0.16969273253588568 1106 8 0.08764395276183023 1106 9 0.14330485583312935 1106 10-11 0.1590111582962237 1106 12-13 0.126034243828812 1106 14-15 0.19629973757744779 1106 16-17 0.16832945992492654 1106 18-19 0.20000509447934434 1106 20-21 0.1532679432086681 1106 22-23 0.18393073198888032 1106 24-25 0.13438633637983344 1106 26-27 0.17172157570163193 1106 28-29 0.166448321653192 1106 30-31 0.1602066117157861 1106 32-33 0.19755631501848114 1106 34-35 0.22741016155926275 1106 36-37 0.21523594764634169 1106 38-39 0.2459233280697859 1106 40-41 0.22505127428886595 1106 42-43 0.18727561806888815 1106 44-45 0.14452653409672678 1106 46-47 0.15473394780513416 1106 48-49 0.2382424587839722 1106 50-51 0.2581824163165365 1106 52-53 0.1998560405521559 1106 54-55 0.18420656099413435 1106 56-57 0.1686932674408581 1106 58-59 0.14717926975121998 1106 60-61 0.12201927134027102 1106 62-63 0.19224162367268605 1106 64-65 0.18496579706143734 1106 66-67 0.25112707739390316 1106 68-69 0.18898070532418387 1106 70-71 0.18125902554561435 1106 72-73 0.12642916047066421 1106 74-75 0.09429055620328342 1106 76-77 0.16479671898281367 1106 78-79 0.18824855346991143 1106 80-81 0.19983321004812638 1106 82-83 0.15980000332887556 1106 84-85 0.14766425456483745 1106 86-87 0.105420686959242 1106 88-89 0.1441807865215452 1106 90-91 0.13476340130180375 1106 92-93 0.15371602349116387 1106 94-95 0.1568805682066028 1106 96-97 0.13342410774438207 1106 98-99 0.13504219203171175 1106 100 0.287720213938055 1107 1 0.100116311735448 1107 2 0.1319964877665356 1107 3 0.11317343598760488 1107 4 0.08936817781648898 1107 5 0.03907787140899899 1107 6 0.038952613849282614 1107 7 0.06696220827743105 1107 8 0.07744740024917007 1107 9 0.050274186315661495 1107 10-11 0.1185759491717846 1107 12-13 0.028296604230291678 1107 14-15 0.14095481371208507 1107 16-17 0.09570371680471368 1107 18-19 0.08207359939228098 1107 20-21 0.13808222132439596 1107 22-23 0.10005849837754965 1107 24-25 0.05016676709308854 1107 26-27 0.11530050668933711 1107 28-29 0.1149313706814894 1107 30-31 0.04983286371790996 1107 32-33 0.14273026807024536 1107 34-35 0.11691589811455572 1107 36-37 0.016131207038519335 1107 38-39 0.061009687882823016 1107 40-41 0.1201162830779694 1107 42-43 0.10851675954556583 1107 44-45 0.03157633997604137 1107 46-47 0.07055944984440998 1107 48-49 0.12861361984034403 1107 50-51 0.19187118723912278 1107 52-53 0.14047733933688988 1107 54-55 0.16592512827887873 1107 56-57 0.04307213878379912 1107 58-59 0.08730995032698985 1107 60-61 0.09455320805977152 1107 62-63 0.08936262548454721 1107 64-65 0.09773499284286657 1107 66-67 0.06063541340007106 1107 68-69 0.07618182476431912 1107 70-71 0.09782774787171888 1107 72-73 0.038521348157523505 1107 74-75 0.09329907459633802 1107 76-77 0.1564239602273716 1107 78-79 0.05694198288869501 1107 80-81 0.023104024794591282 1107 82-83 0.08049160737771643 1107 84-85 0.08609544717489115 1107 86-87 0.009330834024616763 1107 88-89 -0.02010024389177545 1107 90-91 0.01866966044929086 1107 92-93 -0.013359917002176758 1107 94-95 0.012082809989060195 1107 96-97 -0.01086122840039394 1107 98-99 0.08650497735209939 1107 100 0.06941354978660996 1108 1 0.13674969495970402 1108 2 0.13689191227784647 1108 3 0.15021176417620552 1108 4 0.08029779136743542 1108 5 0.08302694138057376 1108 6 0.12553306631510708 1108 7 0.11517312095146082 1108 8 0.060701207024813186 1108 9 0.0495473819902017 1108 10-11 0.028926200049255613 1108 12-13 0.08342331478188214 1108 14-15 0.1032855059344513 1108 16-17 0.10922563603789825 1108 18-19 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5 -0.42593917166576745 2215 6 -0.4193831590191266 2215 7 -0.4179884654004766 2215 8 -0.44244108598943654 2215 9 -0.5907527393057208 2215 10-11 -0.5237618505493344 2215 12-13 -0.5349207656934993 2215 14-15 -0.681935047490775 2215 16-17 -0.6957522981025974 2215 18-19 -0.6838734798271773 2215 20-21 -0.6889555835622403 2215 22-23 -0.6553279112920904 2215 24-25 -0.6567969617797189 2215 26-27 -0.6793335900975919 2215 28-29 -0.6165260722813812 2215 30-31 -0.6433126443244603 2215 32-33 -0.6835541812568309 2215 34-35 -0.6856590947894503 2215 36-37 -0.8016553542357627 2215 38-39 -0.9090755282090797 2215 40-41 -0.8912603840261042 2215 42-43 -0.8980571312231689 2215 44-45 -0.9062501996729893 2215 46-47 -0.9549855462809731 2215 48-49 -0.9530478081491509 2215 50-51 -0.9403855000750738 2215 52-53 -0.9380905267895301 2215 54-55 -0.8791106360633236 2215 56-57 -0.9301659300465701 2215 58-59 -0.9405626854044726 2215 60-61 -0.897511760281958 2215 62-63 -0.8796487124531964 2215 64-65 -0.853306398603145 2215 66-67 -0.9124426993242096 2215 68-69 -0.8992015468762986 2215 70-71 -0.8539675736114489 2215 72-73 -0.8809229376406833 2215 74-75 -0.8838773852457891 2215 76-77 -0.8092645873175641 2215 78-79 -0.9184641404584468 2215 80-81 -0.8873960756671764 2215 82-83 -0.8778196424782436 2215 84-85 -0.7953581922675568 2215 86-87 -0.7636230886113466 2215 88-89 -0.830049147460592 2215 90-91 -0.8055945905326922 2215 92-93 -0.8021431270135224 2215 94-95 -0.7440619220776981 2215 96-97 -0.7112193146489219 2215 98-99 -0.7986263599556338 2215 100 -0.44222869352351424 2216 1 -0.32668407113161635 2216 2 -0.20324685013422794 2216 3 -0.25437374436524607 2216 4 -0.3404529228776667 2216 5 -0.33395947584140373 2216 6 -0.37769048776045366 2216 7 -0.3663086912003237 2216 8 -0.410578666152702 2216 9 -0.4920218045053275 2216 10-11 -0.5346605220436516 2216 12-13 -0.5072647425536871 2216 14-15 -0.651450226918044 2216 16-17 -0.6104025691836554 2216 18-19 -0.6234092709879135 2216 20-21 -0.5529620361953889 2216 22-23 -0.5834238756735601 2216 24-25 -0.6536712649291303 2216 26-27 -0.7178110072105568 2216 28-29 -0.5961212343949143 2216 30-31 -0.6027724962999201 2216 32-33 -0.5475277389803708 2216 34-35 -0.583050882497659 2216 36-37 -0.7308067891098133 2216 38-39 -0.8306922213699366 2216 40-41 -0.8805478972949032 2216 42-43 -0.8077976456719185 2216 44-45 -0.8594383245553345 2216 46-47 -0.7930717404277559 2216 48-49 -0.8482746026031478 2216 50-51 -0.8398578961319956 2216 52-53 -0.8391506923021623 2216 54-55 -0.7779220613266133 2216 56-57 -0.6687868216993422 2216 58-59 -0.8202603820357055 2216 60-61 -0.7331061012347888 2216 62-63 -0.7741170687302841 2216 64-65 -0.6850036868441904 2216 66-67 -0.6984533915944979 2216 68-69 -0.6808328199031308 2216 70-71 -0.7001008258933084 2216 72-73 -0.6440693127800543 2216 74-75 -0.6259404942255529 2216 76-77 -0.6524374678440026 2216 78-79 -0.6871680382798431 2216 80-81 -0.7319853829333383 2216 82-83 -0.6942309192615284 2216 84-85 -0.6872816796859382 2216 86-87 -0.6941733226361961 2216 88-89 -0.6773934565657385 2216 90-91 -0.6726338762194075 2216 92-93 -0.7099334751452204 2216 94-95 -0.72320814416112 2216 96-97 -0.7564320495591375 2216 98-99 -0.7419501052131139 2216 100 -0.21871252452720213 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 21.0 13 119.0 14 482.0 15 1423.0 16 3387.0 17 6665.0 18 11722.0 19 17611.0 20 24166.0 21 32571.0 22 44194.0 23 48150.0 24 46886.0 25 44106.0 26 45483.0 27 54335.0 28 68205.0 29 85634.0 30 106465.0 31 133584.0 32 168881.0 33 217525.0 34 313326.0 35 457409.0 36 729800.0 37 1380133.0 38 1171843.0 39 500402.0 40 667.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.42091584944491 22.564698994627832 12.777214853661212 25.237170302266048 2 14.155442347511677 21.746239423638727 40.214329623225794 23.8839886056238 3 16.461829749700666 29.691977371908614 29.985335588607008 23.860857289783713 4 11.081980649550916 18.244431426222143 41.04752322482966 29.626064699397283 5 11.789844309935496 39.673809577136716 35.76262514558323 12.773720967344563 6 27.31644327096451 40.74667618515203 18.897325463085686 13.039555080797768 7 24.06977332174084 33.25486995802242 26.037458402542136 16.637898317694606 8 25.66090022793615 34.23866228933141 20.895990111976893 19.20444737075555 9 26.94442553773737 13.816444122573552 22.376551499449626 36.86257884023945 10-11 24.907496626975416 26.16427745185337 28.295805026493397 20.63242089467782 12-13 25.705497845738652 24.495816757605702 28.508609696555702 21.290075700099944 14-15 22.856468647704983 24.977393547560695 26.815937936133917 25.350199868600402 16-17 20.98259255105292 26.92763696509499 28.246471643934584 23.843298839917505 18-19 20.357377069832776 27.23926703476136 31.11564327802581 21.287712617380052 20-21 21.948429774161767 26.467014172914777 29.901847652845564 21.682708400077896 22-23 22.617663048185392 26.104550726772846 29.44673648169958 21.83104974334218 24-25 22.055915132934174 26.197137211543186 30.4721167091878 21.274830946334834 26-27 21.886148456474906 26.57709786731761 29.46862549094983 22.068128185257656 28-29 21.06963242036976 27.320230606224072 29.730611210777163 21.879525762629005 30-31 22.02107818855588 26.71547454969619 29.695686780350705 21.56776048139723 32-33 22.72971517867188 27.109922895046314 28.6459766828685 21.514385243413304 34-35 22.395107290264885 26.617910108785402 29.827528254931547 21.15945434601817 36-37 22.213941181730043 26.343168923135984 29.49974427828122 21.943145616852753 38-39 22.419857093290048 26.08436769546177 30.27992735858449 21.215847852663696 40-41 21.70517481724602 25.592363657805674 30.65998424901189 22.04247727593642 42-43 22.009600019036967 25.86374537920565 29.466744540914334 22.659910060843046 44-45 21.96209509488474 27.184023577839923 29.215572096639885 21.638309230635443 46-47 22.10138163216421 26.626046311264513 28.46327956849081 22.809292488080462 48-49 23.259635669601593 26.02264628848314 28.534370731227636 22.18334731068763 50-51 22.54626568428 26.330150999169405 27.794492473312822 23.329090843237772 52-53 21.477159929920177 27.212132845114525 27.835523429901016 23.475183795064282 54-55 21.849824249277848 27.21482741539793 27.449692460294894 23.48565587502933 56-57 23.222681562857765 26.149772265068027 27.413805683338648 23.21374048873556 58-59 21.815795993698202 26.548582060877703 28.615029125860254 23.02059281956384 60-61 22.09825838024481 27.58795541081086 27.27794859914715 23.035837609797177 62-63 22.744272857086116 26.53924265427771 26.797475222638873 23.919009265997303 64-65 21.498587799469274 26.741235730580627 27.482995327892635 24.27718114205747 66-67 22.473469823400446 26.050542501649115 27.443164619199013 24.032823055751425 68-69 21.651712093213934 26.14983350530174 27.92078733244016 24.277667069044163 70-71 21.801313154404255 25.835244256535173 27.908043672195546 24.455398916865022 72-73 22.437957739541574 25.908865114763724 28.43986284239316 23.213314303301544 74-75 22.40696041678514 26.500553351384692 27.787626800579158 23.304859431251014 76-77 21.963148566412865 26.36032405913258 27.51449360883183 24.162033765622727 78-79 21.494582163061423 26.552055257597463 27.66254210704235 24.290820472298762 80-81 21.205716968286485 26.90742768796946 28.344770967649936 23.54208437609412 82-83 22.07422596281458 25.42098723806021 29.432257540728692 23.072529258396518 84-85 22.560840859903866 26.264282753507885 27.515893152939437 23.658983233648815 86-87 20.688796904113715 26.99460753496336 28.25820352299317 24.058392037929753 88-89 21.765050268608412 25.72138808163568 27.935616217603698 24.577945432152205 90-91 21.339045705686086 25.54250535895788 28.344762219045116 24.773686716310916 92-93 21.80015841973601 25.93424163681497 27.842898503761116 24.422701439687906 94-95 20.23330079435582 25.996304239381423 28.56872650234104 25.201668463921717 96-97 20.708358784482844 25.710522314453904 28.113335375840936 25.467783525222316 98-99 19.87877233278223 26.808122274700242 27.977320816762745 25.335784575754783 100 20.841523546933406 25.798336821268602 26.944566953562692 26.4155726782353 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 510.0 1 333.5 2 225.0 3 286.5 4 208.5 5 171.5 6 305.0 7 632.0 8 1021.5 9 1069.0 10 825.5 11 712.0 12 759.0 13 876.0 14 1089.5 15 1483.5 16 2038.0 17 2737.0 18 3709.5 19 4981.5 20 6404.5 21 7997.0 22 9658.5 23 11195.0 24 12734.5 25 14314.0 26 16594.0 27 18974.5 28 23280.5 29 35381.5 30 53901.0 31 70853.5 32 94405.5 33 117206.0 34 134408.5 35 153786.5 36 171294.5 37 203956.0 38 213398.5 39 190560.5 40 169995.0 41 150550.0 42 146199.5 43 154174.5 44 168697.0 45 206849.5 46 239891.0 47 262188.0 48 277583.0 49 263816.5 50 243859.0 51 242976.0 52 242577.0 53 232579.0 54 224335.5 55 193117.5 56 165858.0 57 132858.0 58 97007.0 59 94304.0 60 76756.0 61 48161.0 62 33504.5 63 27586.5 64 19588.0 65 7470.0 66 2760.0 67 1674.5 68 1222.0 69 870.0 70 614.5 71 503.5 72 375.5 73 269.5 74 192.5 75 122.5 76 62.5 77 18.5 78 4.0 79 2.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.007838749915357249 3 0.0 4 0.09189534498985774 5 0.004426794037020946 6 3.4994419264987715E-5 7 6.998883852997543E-5 8 0.0 9 0.0 10-11 0.0 12-13 2.2746372522242014E-4 14-15 0.009929666466440263 16-17 0.0 18-19 1.7497209632493858E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.7497209632493858E-5 60-61 0.0 62-63 0.0 64-65 5.249162889748157E-5 66-67 1.2248046742745702E-4 68-69 0.0 70-71 4.8117326489358113E-4 72-73 3.4994419264987715E-4 74-75 3.8493861191486484E-4 76-77 9.623465297871621E-5 78-79 1.7497209632493858E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.06305994351550787 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5715197.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.715871631345955 #Duplication Level Percentage of deduplicated Percentage of total 1 71.46105054130774 44.817420723897825 2 16.722989726829514 20.975937540003137 3 5.9279048840112 11.153211652454255 4 2.6198891092044656 6.572345162449142 5 1.3274023068920244 4.162459634109634 6 0.7113248961844959 2.6766816526372406 7 0.43134604608545063 1.8936570278488631 8 0.2544250254562511 1.276518978905294 9 0.154762083491124 0.8735435065466081 >10 0.3804079873941228 3.2074372292760986 >50 0.0030969840254001335 0.13665070712525332 >100 0.004051380946142073 0.5252979876061217 >500 6.745141323657892E-4 0.2922372850925397 >1k 5.554821503237249E-4 0.5227125122719288 >5k 3.967729645168734E-5 0.20795527301989666 >10k+ 7.935459290337468E-5 0.7059331267561861 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17133 0.2997796926335173 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11236 0.196598647430701 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8357 0.14622418089875117 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 1.7497209632493858E-5 0.0 0.0 2.6245814448740786E-5 0.0 20-21 1.7497209632493858E-5 0.0 0.0 1.0498325779496315E-4 0.0 22-23 1.7497209632493858E-5 0.0 0.0 3.849386119148649E-4 0.0 24-25 1.7497209632493858E-5 0.0 0.0 7.173855949322482E-4 0.0 26-27 1.7497209632493858E-5 0.0 0.0 0.001461017004313237 0.0 28-29 1.7497209632493858E-5 0.0 0.0 0.005476626614970578 0.0 30-31 1.7497209632493858E-5 0.0 0.0 0.01902821547533707 0.0 32-33 1.7497209632493858E-5 0.0 0.0 0.0445741415387781 0.0 34-35 1.7497209632493858E-5 0.0 0.0 0.0813357789766477 0.0 36-37 1.7497209632493858E-5 0.0 0.0 0.13894534169163372 0.0 38-39 1.7497209632493858E-5 0.0 0.0 0.23092817272965394 0.0 40-41 1.7497209632493858E-5 0.0 0.0 0.34368019160144436 0.0 42-43 1.7497209632493858E-5 0.0 0.0 0.4590305461036601 0.0 44-45 1.7497209632493858E-5 0.0 0.0 0.585334153835817 0.0 46-47 1.7497209632493858E-5 0.0 0.0 0.7388896655705831 0.0 48-49 1.7497209632493858E-5 0.0 0.0 0.9207381652810918 0.0 50-51 1.7497209632493858E-5 0.0 0.0 1.1242569591214442 0.0 52-53 1.7497209632493858E-5 0.0 0.0 1.328213183202609 0.0 54-55 1.7497209632493858E-5 0.0 0.0 1.5313995300599437 0.0 56-57 1.7497209632493858E-5 0.0 0.0 1.7779789568058635 0.0 58-59 1.7497209632493858E-5 0.0 0.0 2.081144359503268 0.0 60-61 1.7497209632493858E-5 0.0 0.0 2.40681117378806 0.0 62-63 1.7497209632493858E-5 0.0 0.0 2.752696013803199 0.0 64-65 1.7497209632493858E-5 0.0 0.0 3.1025527204049133 0.0 66-67 1.7497209632493858E-5 0.0 0.0 3.4863977567177473 0.0 68-69 1.7497209632493858E-5 0.0 0.0 3.9070481734925324 0.0 70-71 1.7497209632493858E-5 0.0 0.0 4.3787554479749335 0.0 72-73 1.7497209632493858E-5 0.0 0.0 4.895588026099538 0.0 74-75 1.7497209632493858E-5 0.0 0.0 5.392640008734607 0.0 76-77 1.7497209632493858E-5 0.0 0.0 5.942166123057525 0.0 78-79 1.7497209632493858E-5 8.748604816246929E-6 0.0 6.523878354499416 0.0 80-81 1.7497209632493858E-5 1.7497209632493858E-5 0.0 7.170443993444145 0.0 82-83 1.7497209632493858E-5 1.7497209632493858E-5 0.0 7.873298155776608 0.0 84-85 1.7497209632493858E-5 1.7497209632493858E-5 0.0 8.559433734305221 0.0 86-87 1.7497209632493858E-5 1.7497209632493858E-5 0.0 9.268201953493467 0.0 88 1.7497209632493858E-5 1.7497209632493858E-5 0.0 9.819241576449595 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 20985 0.0 44.768814 1 GGTATCA 10925 0.0 44.266804 1 GGGCACC 5845 0.0 38.941048 94 GTGGTAT 1785 0.0 36.89744 1 CAACGCA 24855 0.0 36.457054 5 ATCAACG 25325 0.0 35.830364 3 TATCAAC 27365 0.0 34.21087 2 AACGCAG 29355 0.0 32.147778 6 CTGTGCG 7660 0.0 31.787598 9 TAGTACT 7835 0.0 31.40011 4 TACCCCG 315 0.0 31.332645 5 ACTGTGC 8135 0.0 30.850119 8 ACGCAGA 29670 0.0 30.47592 7 GTACTAG 5810 0.0 30.445154 1 CGCAGAG 29860 0.0 30.219309 8 TATTCTC 3905 0.0 29.727901 5 GTACTGT 8390 0.0 29.240032 6 TCAACGC 32320 0.0 28.352152 4 TAGGCAT 7730 0.0 28.21154 5 GCAGAGT 32095 0.0 27.856712 9 >>END_MODULE