FastQCFastQC Report
Mon 16 Jan 2017
ERR1146645_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146645_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427121
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC66650.043.68239294
GTACTAG61550.036.360631
CTGTGCG88100.035.1493959
TAGTACT92750.033.897884
ACTGTGC95450.033.0827568
CAACGCA88950.033.019195
GCATCGT64200.032.644368
TCTAACG32100.032.5027662
TAGGCAT79600.032.233835
CTAACGC32300.032.1554153
GTATCAA105900.031.8772561
GTACTGT99500.031.4058746
CGCCTAC32350.031.3755847
TAACGCC33400.031.2368244
ATCAACG96200.030.5329883
CTAGTAC107850.030.4158293
TATTCTC29750.030.32825
TACTGTG108650.030.3180057
CAGTACG10600.029.7014269
CTAGGCA89800.029.359384
Produced by FastQC (version 0.11.5)