##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146645_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5427121 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.285094435889672 31.0 31.0 34.0 28.0 34.0 2 31.410743559983278 31.0 31.0 34.0 28.0 34.0 3 31.54722660504529 33.0 31.0 34.0 28.0 34.0 4 34.971065874521685 37.0 35.0 37.0 32.0 37.0 5 35.12874100282636 37.0 35.0 37.0 32.0 37.0 6 35.205635363574906 37.0 35.0 37.0 32.0 37.0 7 35.128965246951374 37.0 35.0 37.0 32.0 37.0 8 35.10395824968708 37.0 35.0 37.0 32.0 37.0 9 36.589130037823 39.0 37.0 39.0 32.0 39.0 10-11 36.425614335851364 39.0 37.0 39.0 31.5 39.0 12-13 36.34280413132488 39.0 37.0 39.0 31.0 39.0 14-15 37.5021188029528 40.0 37.0 41.0 31.0 41.0 16-17 37.52905481561955 40.0 37.0 41.0 31.0 41.0 18-19 37.50231568450381 40.0 37.0 41.0 31.0 41.0 20-21 37.5666635219668 40.0 37.0 41.0 31.0 41.0 22-23 37.50411654724485 40.0 37.0 41.0 31.0 41.0 24-25 37.46295475999153 40.0 37.0 41.0 31.0 41.0 26-27 37.50062067899352 40.0 37.0 41.0 31.0 41.0 28-29 37.49016154237209 40.0 37.0 41.0 31.0 41.0 30-31 37.356205435625995 40.0 36.0 41.0 30.0 41.0 32-33 37.34618078351303 40.0 36.0 41.0 30.0 41.0 34-35 37.280352879547 40.0 36.0 41.0 30.0 41.0 36-37 37.17112470866229 40.0 36.0 41.0 30.0 41.0 38-39 37.06264739628986 40.0 36.0 41.0 30.0 41.0 40-41 36.96700958021758 40.0 35.5 41.0 30.0 41.0 42-43 36.80767040204189 39.5 35.0 41.0 30.0 41.0 44-45 36.65246444661912 39.0 35.0 41.0 29.5 41.0 46-47 36.64362062316282 39.0 35.0 41.0 29.5 41.0 48-49 36.49953760013827 39.0 35.0 41.0 29.0 41.0 50-51 36.314849622847916 39.0 35.0 41.0 29.0 41.0 52-53 36.09183248355804 38.5 35.0 40.5 28.0 41.0 54-55 35.87293364198071 38.0 34.5 40.0 28.0 41.0 56-57 35.62598539815124 37.5 34.0 40.0 28.0 41.0 58-59 35.31963392745435 37.0 34.0 40.0 28.0 41.0 60-61 35.07217141464139 36.5 34.0 40.0 27.5 41.0 62-63 34.76046821141449 36.0 33.0 39.5 27.0 41.0 64-65 34.60218309118223 35.5 33.0 39.0 27.0 41.0 66-67 34.34617571636969 35.0 33.0 39.0 26.5 41.0 68-69 34.214102744346405 35.0 33.0 39.0 26.0 41.0 70-71 34.14249018954986 35.0 33.0 38.0 27.0 41.0 72-73 33.92229839725335 35.0 33.0 37.0 26.5 40.0 74-75 33.67292372880575 35.0 33.0 37.0 26.0 39.5 76-77 33.421578955029744 35.0 33.0 36.0 26.0 39.0 78-79 33.1721571160842 35.0 33.0 36.0 26.0 39.0 80-81 32.9968700716273 35.0 33.0 36.0 26.0 38.5 82-83 32.74356541893943 35.0 33.0 35.0 25.5 37.0 84-85 32.58039888920848 35.0 33.0 35.0 25.0 37.0 86-87 32.418840947161485 35.0 32.0 35.0 25.0 36.5 88-89 32.29565021675397 35.0 32.0 35.0 25.0 36.0 90-91 32.15358677280275 35.0 32.0 35.0 25.0 36.0 92-93 32.00310671164324 35.0 32.0 35.0 25.0 36.0 94-95 31.942220193727024 35.0 32.0 35.0 25.0 35.5 96-97 31.839677058978417 35.0 32.0 35.0 24.5 35.0 98-99 31.750924385139008 35.0 32.0 35.0 24.0 35.0 100 30.80226108833763 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32146170824077913 1101 2 0.37189724478541564 1101 3 0.44641536166331974 1101 4 0.44809957428313396 1101 5 0.36219210198797214 1101 6 0.38121906268179373 1101 7 0.44738161847591584 1101 8 0.42609560553484727 1101 9 0.5656691089195292 1101 10-11 0.5461489421042174 1101 12-13 0.5383308450071524 1101 14-15 0.7125296840202893 1101 16-17 0.6562149741238699 1101 18-19 0.6082067969778606 1101 20-21 0.6092340774103491 1101 22-23 0.665272529292416 1101 24-25 0.6586069196475037 1101 26-27 0.5904282213278904 1101 28-29 0.5966782819680887 1101 30-31 0.6880840763208838 1101 32-33 0.6191186055875306 1101 34-35 0.6604251061861461 1101 36-37 0.6709156658759099 1101 38-39 0.6507899303137279 1101 40-41 0.6053787276002325 1101 42-43 0.5707326116243863 1101 44-45 0.5662910095529412 1101 46-47 0.5730034699916828 1101 48-49 0.543757874719212 1101 50-51 0.5339566070941686 1101 52-53 0.48425932091925006 1101 54-55 0.5052023078908761 1101 56-57 0.4880166711547389 1101 58-59 0.49086930804652695 1101 60-61 0.4698823389878015 1101 62-63 0.4811288205960338 1101 64-65 0.4824641884060057 1101 66-67 0.508164733200239 1101 68-69 0.5097504825051118 1101 70-71 0.5426962421594439 1101 72-73 0.5321226444309559 1101 74-75 0.48541631008549047 1101 76-77 0.4998784155286842 1101 78-79 0.4605735967409146 1101 80-81 0.41690781755399087 1101 82-83 0.4419577175097018 1101 84-85 0.47046185474059854 1101 86-87 0.3957795088613949 1101 88-89 0.4369729565976854 1101 90-91 0.4293925764882829 1101 92-93 0.44731400891712525 1101 94-95 0.41896930196981685 1101 96-97 0.3932676856824173 1101 98-99 0.3978441320849697 1101 100 0.4769750688980352 1102 1 0.22710075585581535 1102 2 0.3675833558146486 1102 3 0.3737936454932793 1102 4 0.4338661081204549 1102 5 0.34099288558282836 1102 6 0.366748010260757 1102 7 0.33948325561273407 1102 8 0.3763461285006571 1102 9 0.5202914436185822 1102 10-11 0.6003811871165681 1102 12-13 0.5037636585796079 1102 14-15 0.7425148898542986 1102 16-17 0.6612394462570848 1102 18-19 0.5846802130967674 1102 20-21 0.5752863791383476 1102 22-23 0.6231314504037684 1102 24-25 0.6506102331289583 1102 26-27 0.5382220799242958 1102 28-29 0.5195302153859203 1102 30-31 0.556694571008407 1102 32-33 0.5140283036265387 1102 34-35 0.6086281203220096 1102 36-37 0.593760253676848 1102 38-39 0.6220651294056836 1102 40-41 0.4939530182218448 1102 42-43 0.5478916732381975 1102 44-45 0.5231872337654977 1102 46-47 0.45747923390074874 1102 48-49 0.4164026570620365 1102 50-51 0.44092160873874775 1102 52-53 0.39719675357468276 1102 54-55 0.42886901994128834 1102 56-57 0.43232101082355 1102 58-59 0.36388427205066876 1102 60-61 0.3891930300419162 1102 62-63 0.3873282873136077 1102 64-65 0.38740308644182164 1102 66-67 0.38216279339594195 1102 68-69 0.3796962185148729 1102 70-71 0.34305645811122076 1102 72-73 0.3301713772184698 1102 74-75 0.2700177682606224 1102 76-77 0.2889356687639122 1102 78-79 0.30294620292368535 1102 80-81 0.3252471045799936 1102 82-83 0.3713080655375407 1102 84-85 0.34757178961725543 1102 86-87 0.3671563535118878 1102 88-89 0.37557721452896686 1102 90-91 0.37792036514412786 1102 92-93 0.40515627372520413 1102 94-95 0.397503725074543 1102 96-97 0.36979986673642884 1102 98-99 0.2994675602048318 1102 100 0.32376716314276877 1103 1 0.22081321718236424 1103 2 0.291905370456508 1103 3 0.3328747344240206 1103 4 0.3696975634027666 1103 5 0.22469771071012445 1103 6 0.3221565954690959 1103 7 0.30809868662663575 1103 8 0.33041582841039485 1103 9 0.38931525826668434 1103 10-11 0.5547864858299292 1103 12-13 0.422122809968549 1103 14-15 0.5758956316543546 1103 16-17 0.5930343096493544 1103 18-19 0.6439282739748435 1103 20-21 0.5328989783061786 1103 22-23 0.5726818465797763 1103 24-25 0.5743117881299469 1103 26-27 0.49950464058572663 1103 28-29 0.5296758732870117 1103 30-31 0.4850266809556416 1103 32-33 0.5553396774171304 1103 34-35 0.5447372033906035 1103 36-37 0.5079412684368307 1103 38-39 0.5947413280854192 1103 40-41 0.44158997719004134 1103 42-43 0.4800594979966917 1103 44-45 0.417108152933082 1103 46-47 0.4755108199290703 1103 48-49 0.4925181517956645 1103 50-51 0.4492045181939659 1103 52-53 0.43201237195248154 1103 54-55 0.43042224793056505 1103 56-57 0.44243612097537266 1103 58-59 0.4496313040149431 1103 60-61 0.41816221924576524 1103 62-63 0.3581539320449778 1103 64-65 0.3535613549130616 1103 66-67 0.3540645061725627 1103 68-69 0.34567443158126565 1103 70-71 0.35140410419268875 1103 72-73 0.2824980102535761 1103 74-75 0.24806054632821173 1103 76-77 0.28339825980152966 1103 78-79 0.30208796288275863 1103 80-81 0.24791573561864766 1103 82-83 0.2784127372323084 1103 84-85 0.3303197001430149 1103 86-87 0.32496741054077205 1103 88-89 0.31808411979643836 1103 90-91 0.31184358248653865 1103 92-93 0.29919597238285434 1103 94-95 0.30731722356155444 1103 96-97 0.30583323239051197 1103 98-99 0.30910532999639884 1103 100 0.42638964108368427 1104 1 0.2165052132565073 1104 2 0.28505574072083206 1104 3 0.24468151972337182 1104 4 0.39363657480975434 1104 5 0.30566037452611283 1104 6 0.2511853579405212 1104 7 0.32065732379064826 1104 8 0.34181877771229097 1104 9 0.3657818686917125 1104 10-11 0.4955175692610041 1104 12-13 0.3910822358228856 1104 14-15 0.4835147047336008 1104 16-17 0.48302680774781237 1104 18-19 0.43012808304415273 1104 20-21 0.4296306279911022 1104 22-23 0.445162134480519 1104 24-25 0.5157379351279587 1104 26-27 0.41307228823929876 1104 28-29 0.39437038021971915 1104 30-31 0.39292993601382165 1104 32-33 0.41024366057138906 1104 34-35 0.451920484739496 1104 36-37 0.38292026626216114 1104 38-39 0.43857950609104535 1104 40-41 0.5019617435707957 1104 42-43 0.48252385659450425 1104 44-45 0.4057121313039147 1104 46-47 0.42481192325675465 1104 48-49 0.3788890130949909 1104 50-51 0.30073133459445955 1104 52-53 0.3262072987269846 1104 54-55 0.4555557204452185 1104 56-57 0.4298311312418974 1104 58-59 0.4099490050690022 1104 60-61 0.3537956371536026 1104 62-63 0.4202992757358146 1104 64-65 0.3476485280609225 1104 66-67 0.29674671083078863 1104 68-69 0.3422972415542418 1104 70-71 0.2959227937736699 1104 72-73 0.28105023526302375 1104 74-75 0.20146191649310197 1104 76-77 0.2512993575839886 1104 78-79 0.22878597076491758 1104 80-81 0.26002260008276323 1104 82-83 0.28719610307243926 1104 84-85 0.2868175615867372 1104 86-87 0.29067224212625575 1104 88-89 0.23996849673699217 1104 90-91 0.2751838434408782 1104 92-93 0.2909427092666945 1104 94-95 0.3302659191102606 1104 96-97 0.25164209046816666 1104 98-99 0.21872909933956208 1104 100 0.2331007918393233 1105 1 0.1084148840535164 1105 2 0.19521166615498942 1105 3 0.2704159485191795 1105 4 0.3048969786721898 1105 5 0.2832925346210544 1105 6 0.24856507126492033 1105 7 0.25673769925399625 1105 8 0.2880933779927375 1105 9 0.38211789380836336 1105 10-11 0.38386058366973685 1105 12-13 0.2825843641406678 1105 14-15 0.4287805132535212 1105 16-17 0.3898886852968815 1105 18-19 0.4040937928911532 1105 20-21 0.393482025164019 1105 22-23 0.3683086499963153 1105 24-25 0.39291776181667615 1105 26-27 0.4102265567086505 1105 28-29 0.4531050929424367 1105 30-31 0.45898362839608353 1105 32-33 0.3978087989996766 1105 34-35 0.4431980376184228 1105 36-37 0.4015492603009534 1105 38-39 0.3957583301887695 1105 40-41 0.40909833773924476 1105 42-43 0.41492004455630394 1105 44-45 0.4203726620051569 1105 46-47 0.4351187650955666 1105 48-49 0.2952239143676181 1105 50-51 0.3796758304680523 1105 52-53 0.3876114681185143 1105 54-55 0.3592835924762241 1105 56-57 0.4246017745228059 1105 58-59 0.3921107072649619 1105 60-61 0.3957055979983224 1105 62-63 0.3561480055747168 1105 64-65 0.35331720892843066 1105 66-67 0.33478327710958666 1105 68-69 0.2916742401483603 1105 70-71 0.2876312310668183 1105 72-73 0.26539375777138474 1105 74-75 0.2722266597819001 1105 76-77 0.27177021275382174 1105 78-79 0.3363553481060464 1105 80-81 0.2950341431821002 1105 82-83 0.31051620087681187 1105 84-85 0.2501863455353046 1105 86-87 0.22503746151201653 1105 88-89 0.2081372141330604 1105 90-91 0.2891070714023769 1105 92-93 0.28004607466777287 1105 94-95 0.2657162876794885 1105 96-97 0.18788942873519687 1105 98-99 0.19328188320369577 1105 100 0.33077331959728795 1106 1 0.12019439799271225 1106 2 0.23668521764163586 1106 3 0.20133038043253393 1106 4 0.2875787116896973 1106 5 0.18689355775150318 1106 6 0.24458515898377442 1106 7 0.1934183336040789 1106 8 0.18210209765336316 1106 9 0.3045640349030805 1106 10-11 0.33989609690178924 1106 12-13 0.2748667371510649 1106 14-15 0.4681177502168694 1106 16-17 0.35438743400325734 1106 18-19 0.39411127575137783 1106 20-21 0.39061767941003467 1106 22-23 0.3968286420540821 1106 24-25 0.40289736083087746 1106 26-27 0.4511735303802169 1106 28-29 0.40822435584419026 1106 30-31 0.49166992286857436 1106 32-33 0.3931885020060051 1106 34-35 0.3464718059807126 1106 36-37 0.4094530582222049 1106 38-39 0.3814753042580463 1106 40-41 0.4124311385489179 1106 42-43 0.37123818441536827 1106 44-45 0.3524718392019821 1106 46-47 0.3434968830939056 1106 48-49 0.2999612240868217 1106 50-51 0.301439876834678 1106 52-53 0.3392749579328367 1106 54-55 0.29912650909149363 1106 56-57 0.3007889738402696 1106 58-59 0.3162919411665541 1106 60-61 0.3647153783907342 1106 62-63 0.3704935451533302 1106 64-65 0.3516310255502191 1106 66-67 0.3943526321932893 1106 68-69 0.3360018554488775 1106 70-71 0.3077783330920596 1106 72-73 0.28554932647083575 1106 74-75 0.28384603986763324 1106 76-77 0.2713498588197183 1106 78-79 0.3126629632588802 1106 80-81 0.3062180101508716 1106 82-83 0.29136596753842525 1106 84-85 0.3007687164043844 1106 86-87 0.25826998662184053 1106 88-89 0.28351026052504835 1106 90-91 0.28474710409031445 1106 92-93 0.333503847287723 1106 94-95 0.37219829261972137 1106 96-97 0.3503380202333872 1106 98-99 0.28697540146180245 1106 100 0.2718799695383005 1107 1 0.08492551550935801 1107 2 0.12286138665613677 1107 3 0.12893993319473296 1107 4 0.22481035562992702 1107 5 0.1375704297575595 1107 6 0.242250508536344 1107 7 0.1176972342865028 1107 8 0.12796388456949614 1107 9 0.19285800281247845 1107 10-11 0.30349141123117107 1107 12-13 0.23748874766443606 1107 14-15 0.36148253148270015 1107 16-17 0.18836080536250677 1107 18-19 0.17591429082650478 1107 20-21 0.21634015424731956 1107 22-23 0.369232847274823 1107 24-25 0.42011835915396034 1107 26-27 0.2790269603490003 1107 28-29 0.3079879224627433 1107 30-31 0.39303136979105346 1107 32-33 0.37893847999420416 1107 34-35 0.3061716693912544 1107 36-37 0.3331368359500857 1107 38-39 0.3726550749054951 1107 40-41 0.3492227485110888 1107 42-43 0.38651634298575743 1107 44-45 0.4042893311519151 1107 46-47 0.3798643357095557 1107 48-49 0.3496104164157714 1107 50-51 0.35330802969781416 1107 52-53 0.3390846138638821 1107 54-55 0.26478978323207514 1107 56-57 0.27315185078786897 1107 58-59 0.30293357629969364 1107 60-61 0.28426811175594224 1107 62-63 0.34719249202897373 1107 64-65 0.3106365962587745 1107 66-67 0.37763863302289025 1107 68-69 0.33425090591892115 1107 70-71 0.2978396216535728 1107 72-73 0.3564787837331025 1107 74-75 0.3512573216671626 1107 76-77 0.2782274016420132 1107 78-79 0.3186051666091103 1107 80-81 0.24627756938810563 1107 82-83 0.2486858057628254 1107 84-85 0.29342321829512485 1107 86-87 0.3042385737633566 1107 88-89 0.2816773578791256 1107 90-91 0.20313285349998722 1107 92-93 0.2609307797853191 1107 94-95 0.2464648637702993 1107 96-97 0.23526454874605918 1107 98-99 0.23899389619137423 1107 100 0.33377550088498964 1108 1 0.07481924000121509 1108 2 0.14299314785726835 1108 3 0.10366039594442711 1108 4 0.17632792173221645 1108 5 0.081318732020911 1108 6 0.13502296299771643 1108 7 0.1381110162039505 1108 8 0.15686247721596658 1108 9 0.17448389012541554 1108 10-11 0.2969319250644915 1108 12-13 0.1801235451483123 1108 14-15 0.2861985604738706 1108 16-17 0.2613284672676599 1108 18-19 0.24202357371375882 1108 20-21 0.23351243074099415 1108 22-23 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-3.419831064139551 2215 32-33 -1.359915661989639 2215 34-35 -1.1782389205782025 2215 36-37 -0.9726604261377361 2215 38-39 -0.7871786525179516 2215 40-41 -0.8754116333009208 2215 42-43 -0.7877733800880193 2215 44-45 -0.7877686194203193 2215 46-47 -0.8784473278836344 2215 48-49 -0.9315552715322823 2215 50-51 -0.8682653919969994 2215 52-53 -0.8496341074895923 2215 54-55 -0.7295371914613114 2215 56-57 -0.7840545593233514 2215 58-59 -0.8700953213681402 2215 60-61 -0.8198513312914883 2215 62-63 -0.8564350571605743 2215 64-65 -0.858745239712043 2215 66-67 -0.7518636333510429 2215 68-69 -0.7297909777625051 2215 70-71 -0.8709420268576054 2215 72-73 -0.8146096227650474 2215 74-75 -1.0046311687244511 2215 76-77 -0.8753800274804533 2215 78-79 -0.7960845310978684 2215 80-81 -0.7660129954714279 2215 82-83 -0.7633620271425556 2215 84-85 -0.8062201869654402 2215 86-87 -0.7066083615178052 2215 88-89 -0.6833220547227121 2215 90-91 -0.7648864156034634 2215 92-93 -0.8385832729740272 2215 94-95 -0.7700162401542876 2215 96-97 -1.0278302112374078 2215 98-99 -0.9293753517004433 2215 100 -0.4498913980753727 2216 1 -0.4560035564070688 2216 2 -0.7006677718584875 2216 3 -0.7054847769222263 2216 4 -0.513269346302728 2216 5 -0.4418728745801914 2216 6 -0.3343950755053271 2216 7 -0.583664404764022 2216 8 -0.5716950075640312 2216 9 -0.700420666712894 2216 10-11 -1.033406879070867 2216 12-13 -0.6461760089412394 2216 14-15 -0.7747778485217154 2216 16-17 -0.8097546408198184 2216 18-19 -1.1079825028341972 2216 20-21 -1.1407450532754524 2216 22-23 -0.9439632480620972 2216 24-25 -1.041396705460457 2216 26-27 -1.4219228540820055 2216 28-29 -1.2844815330782424 2216 30-31 -1.428748563752123 2216 32-33 -1.6259332051871525 2216 34-35 -1.2483477216009433 2216 36-37 -1.3432392166955367 2216 38-39 -1.2184406189034291 2216 40-41 -1.1445081790086746 2216 42-43 -1.1218457281227288 2216 44-45 -1.072054018910805 2216 46-47 -1.1422115072250136 2216 48-49 -1.125204616055882 2216 50-51 -1.2102902634232962 2216 52-53 -1.253059868506611 2216 54-55 -1.267226085418038 2216 56-57 -1.2033417698758981 2216 58-59 -1.1091517441859509 2216 60-61 -1.1087366744706628 2216 62-63 -1.2198893026157336 2216 64-65 -1.1928187945057687 2216 66-67 -1.143662271326484 2216 68-69 -1.1692305096237448 2216 70-71 -1.014021199299222 2216 72-73 -0.9556140809391778 2216 74-75 -1.068725242731169 2216 76-77 -1.0660207925016323 2216 78-79 -0.9802318490359525 2216 80-81 -1.0321988261381385 2216 82-83 -1.080032386943298 2216 84-85 -1.0651612830264732 2216 86-87 -1.0257187449340783 2216 88-89 -1.0769652176848652 2216 90-91 -1.138133140992089 2216 92-93 -1.1518444339094387 2216 94-95 -1.1537975899524113 2216 96-97 -1.1183710061258694 2216 98-99 -1.2180633405263457 2216 100 -0.8845684555117437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 1.0 2 9.0 3 35.0 4 43.0 5 39.0 6 50.0 7 174.0 8 542.0 9 823.0 10 1346.0 11 2375.0 12 4345.0 13 6610.0 14 9346.0 15 12897.0 16 15924.0 17 19106.0 18 21979.0 19 25099.0 20 28826.0 21 33448.0 22 38143.0 23 44413.0 24 51540.0 25 59415.0 26 65564.0 27 72640.0 28 82478.0 29 98813.0 30 115240.0 31 133685.0 32 164009.0 33 211756.0 34 305085.0 35 441542.0 36 706515.0 37 1226642.0 38 1034625.0 39 391437.0 40 562.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83527496885012 20.49071698731032 11.616294029733517 24.057714014106036 2 15.883633326785432 19.67343164523979 39.90860700311093 24.53432802486385 3 18.667491973437112 26.60060895551212 28.68918299605154 26.042716074999227 4 12.025885969569353 16.19767481073216 40.54138075089435 31.235058468804137 5 12.913589332863577 38.927725182889496 35.22698271631873 12.931702767928202 6 29.938794598534873 38.73620501450901 18.007684579909387 13.317315807046729 7 27.039661400775554 31.834499863185716 23.75855089260954 17.36728784342918 8 26.57255010061675 34.916397166847645 19.74966413483326 18.761388597702354 9 26.30553002006417 14.753826500293805 21.42643005354438 37.51421342609765 10-11 25.917293314129687 25.771911655490797 27.481887407709593 20.82890762266992 12-13 25.847309242964002 23.65953201765987 27.625070560159454 22.868088179216677 14-15 23.245635087602277 24.917785485783554 24.85067798932158 26.985901437292586 16-17 22.17006415773601 27.486787125262087 26.201780584928247 24.141368132073655 18-19 21.41612975623167 28.542494082407384 27.838012190074956 22.203363971285995 20-21 23.19154865864484 27.54047190310421 26.862380097538864 22.405599340712087 22-23 23.80008476059738 27.205037727678942 26.399157921891263 22.595719589832413 24-25 23.176655619849704 27.133093491707793 27.44410940485477 22.246141483587735 26-27 23.160404250951643 27.320180890618374 26.228430238407523 23.29098462002246 28-29 22.109299975741173 27.916198051167605 26.879766599307654 23.09473537378357 30-31 23.031215613491476 27.632695356829167 26.851860468180305 22.48422856149905 32-33 23.6157025554843 27.70698354082362 25.784915861397963 22.89239804229411 34-35 23.41311421851761 27.42708050311719 26.94203615987797 22.217769118487233 36-37 22.949778666722686 26.9740142091136 26.597760536964003 23.47844658719971 38-39 23.26625467152334 27.255345405723446 27.169751102084245 22.30864882066897 40-41 22.814603544257427 26.126734304707668 27.900641236785322 23.158020914249587 42-43 22.795189773015434 26.49221233288711 27.12297687989102 23.58962101420644 44-45 22.940745915448936 27.527488896672452 27.010345720165645 22.52141946771297 46-47 22.798873579995725 27.280714849134913 26.531955164355587 23.388456406513775 48-49 23.675215108677662 26.87319544802282 26.715100752654603 22.736488690644915 50-51 23.13350273845817 26.59370193323094 26.854729945157118 23.418065383153774 52-53 22.610836911829026 27.309753935761616 26.528600841514006 23.550808310895356 54-55 22.561382243465953 27.179638775717635 26.572388838632076 23.68659014218434 56-57 23.73173049944045 26.547713441533222 26.32826059012508 23.392295468901246 58-59 22.484193811838058 27.085024036979654 27.33336914776189 23.0974130034204 60-61 22.505810940001613 27.775247353689856 26.516435342494155 23.202506363814372 62-63 23.060213665704254 26.989777419656523 26.00312265760368 23.946886257035537 64-65 22.145210753705292 27.30578077125539 26.549376670574365 23.99963180446495 66-67 22.938410381900027 26.800386301713946 26.482104797009615 23.779098519376408 68-69 22.29381838672672 26.946476766329546 26.83575797685252 23.92394687009121 70-71 22.32933305470696 26.562214946978063 26.945750044871374 24.162701953443598 72-73 23.0823833272645 26.60067054482412 27.41644356941838 22.900502558493002 74-75 23.286672196970976 26.906146718549433 26.838791629430176 22.968389455049415 76-77 22.92833160109978 26.89351554956031 26.517519515269456 23.660633334070454 78-79 22.849104425256268 26.894073941594947 26.41437563525864 23.84244599789014 80-81 22.299193630718324 27.34109321104069 27.24348047542581 23.116232682815184 82-83 23.25466075201875 26.205873014673784 28.33250380107589 22.206962432231574 84-85 23.443975316302147 26.808789059386662 26.888297080814482 22.85893854349671 86-87 22.013905243554603 27.57113772821439 27.393189751346426 23.02176727688458 88-89 23.394514601151613 26.163279777380605 27.03779543258737 23.404410188880416 90-91 22.940572934975258 26.32526515254478 27.164539011666665 23.5696229008133 92-93 23.37058580013104 26.568404774205234 27.073267791620953 22.98774163404277 94-95 22.150182302387197 26.633322878848244 27.761246341698026 23.455248477066533 96-97 22.513127549379764 26.55683659921147 27.229923533579715 23.70011231782905 98-99 21.822277812713793 27.348688287068068 27.052682740304107 23.776351159914032 100 22.818484199293675 26.563240144280332 26.063221300045303 24.555054356380694 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 497.0 1 372.0 2 304.5 3 359.0 4 320.0 5 262.5 6 249.5 7 259.0 8 265.5 9 289.5 10 276.0 11 263.5 12 288.0 13 309.5 14 353.5 15 543.0 16 682.5 17 748.0 18 1007.0 19 1295.5 20 1638.5 21 2200.0 22 3110.0 23 4427.0 24 5872.5 25 7669.5 26 10797.0 27 14336.0 28 19000.0 29 29668.5 30 45418.0 31 59622.5 32 78440.5 33 97041.5 34 109798.5 35 125565.0 36 139704.0 37 165271.5 38 176514.0 39 164183.0 40 152051.5 41 138649.0 42 135121.5 43 144020.0 44 160312.5 45 192754.0 46 223617.0 47 245616.0 48 260567.0 49 256753.5 50 244617.5 51 251530.0 52 255848.5 53 242458.0 54 228002.5 55 194287.0 56 169699.0 57 140076.0 58 108819.0 59 108040.5 60 91031.5 61 61619.0 62 46061.0 63 38698.0 64 28349.5 65 13710.0 66 7424.5 67 5186.0 68 3981.5 69 2842.5 70 1940.5 71 1368.5 72 980.0 73 680.5 74 450.5 75 314.5 76 189.0 77 101.0 78 60.0 79 41.5 80 38.0 81 28.5 82 23.5 83 24.5 84 20.0 85 16.5 86 12.5 87 11.0 88 9.5 89 9.5 90 9.5 91 8.0 92 6.0 93 5.0 94 5.0 95 6.0 96 6.5 97 5.0 98 6.5 99 5.5 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06159803697024629 2 0.00823641116533057 3 0.008328541044137397 4 0.01017113862027399 5 0.0038694549098868443 6 0.002745470388443523 7 0.0010318546426364918 8 0.0016951897700456652 9 6.264831758864415E-4 10-11 4.882883576761971E-4 12-13 2.6717664853980594E-4 14-15 1.3819481821024444E-4 16-17 5.159273213182459E-4 18-19 0.0015293559881933716 20-21 9.85789703233077E-4 22-23 0.0013082442790569807 24-25 0.0012437533638922 26-27 0.003593065273466355 28-29 0.004247187412994846 30-31 0.005555431692051826 32-33 0.006651777249853099 34-35 0.0035838522855856725 36-37 0.008531226777512424 38-39 0.0065964993225690015 40-41 0.006310896698267829 42-43 0.01564365342139967 44-45 0.021328066943781056 46-47 0.019918479798036567 48-49 0.0231983034835597 50-51 0.019807923943468368 52-53 0.026238589484185074 54-55 0.02029621230114457 56-57 0.016555739221587283 58-59 0.01847204070076934 60-61 0.01720064837323509 62-63 0.012041375160052633 64-65 0.01209665308733673 66-67 0.014602585790882496 68-69 0.005702839498142754 70-71 0.009194561904921596 72-73 0.009010302147307938 74-75 0.01209665308733673 76-77 0.01107401143258092 78-79 0.012704710287461807 80-81 0.010594936062785408 82-83 0.006780759080182661 84-85 0.01150702186297302 86-87 0.00985789703233077 88-89 0.008475948850228326 90-91 0.014068232493802884 92-93 0.009222200868563644 94-95 0.0077941877470577865 96-97 0.010244842523319454 98-99 0.009830258068688721 100 0.2639521027815669 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5427121.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.0193623358847 #Duplication Level Percentage of deduplicated Percentage of total 1 73.17344555511845 46.84517324358259 2 15.346465282205916 19.649418429532307 3 5.547373709399905 10.654179825438998 4 2.497836497123973 6.396395990607067 5 1.2808557292451714 4.099978351527023 6 0.7446141032160547 2.860183204451907 7 0.4434537387250712 1.9872737905050155 8 0.2637497094737236 1.3508070573426108 9 0.17878798711347607 1.0301303635488999 >10 0.5168085138389859 4.753260321366847 >50 0.005358582312813323 0.229965854068849 >100 0.0012068510919675617 0.12253950220866734 >500 4.374113456213432E-5 0.02069406581919299 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.8425975761365927E-5 7 0.0 0.0 0.0 0.0 1.8425975761365927E-5 8 0.0 0.0 0.0 0.0 1.8425975761365927E-5 9 1.8425975761365927E-5 0.0 0.0 0.0 1.8425975761365927E-5 10-11 1.8425975761365927E-5 0.0 0.0 0.0 1.8425975761365927E-5 12-13 1.8425975761365927E-5 0.0 0.0 0.0 1.8425975761365927E-5 14-15 1.8425975761365927E-5 0.0 0.0 0.0 1.8425975761365927E-5 16-17 1.8425975761365927E-5 0.0 0.0 0.0 1.8425975761365927E-5 18-19 1.8425975761365927E-5 0.0 0.0 1.8425975761365927E-5 1.8425975761365927E-5 20-21 1.8425975761365927E-5 0.0 0.0 5.527792728409778E-5 1.8425975761365927E-5 22-23 1.8425975761365927E-5 0.0 0.0 1.1055585456819555E-4 1.8425975761365927E-5 24-25 1.8425975761365927E-5 0.0 0.0 1.3819481821024444E-4 1.8425975761365927E-5 26-27 1.8425975761365927E-5 0.0 0.0 4.237974425114163E-4 1.8425975761365927E-5 28-29 1.8425975761365927E-5 0.0 0.0 0.0024230158126196193 1.8425975761365927E-5 30-31 1.8425975761365927E-5 0.0 0.0 0.010115860692989892 1.8425975761365927E-5 32-33 1.8425975761365927E-5 0.0 0.0 0.024718446483872388 1.8425975761365927E-5 34-35 1.8425975761365927E-5 0.0 0.0 0.04815628765232985 1.8425975761365927E-5 36-37 1.8425975761365927E-5 0.0 0.0 0.08755102383013019 1.8425975761365927E-5 38-39 1.8425975761365927E-5 0.0 0.0 0.1494162374489163 1.8425975761365927E-5 40-41 1.8425975761365927E-5 0.0 0.0 0.21634859440207801 1.8425975761365927E-5 42-43 1.8425975761365927E-5 0.0 0.0 0.2880256401137915 1.8425975761365927E-5 44-45 1.8425975761365927E-5 0.0 0.0 0.37666379651384224 1.8425975761365927E-5 46-47 1.8425975761365927E-5 0.0 0.0 0.48663923284555477 1.8425975761365927E-5 48-49 1.8425975761365927E-5 0.0 0.0 0.6160540736055083 1.8425975761365927E-5 50-51 1.8425975761365927E-5 0.0 0.0 0.753981715167213 1.8425975761365927E-5 52-53 1.8425975761365927E-5 0.0 0.0 0.89140264239548 1.8425975761365927E-5 54-55 1.8425975761365927E-5 0.0 0.0 1.0408465188080385 1.8425975761365927E-5 56-57 1.8425975761365927E-5 0.0 0.0 1.2242955334881975 1.8425975761365927E-5 58-59 1.8425975761365927E-5 0.0 0.0 1.4516997133470952 1.8425975761365927E-5 60-61 1.8425975761365927E-5 0.0 0.0 1.6840881196494422 1.8425975761365927E-5 62-63 1.8425975761365927E-5 0.0 0.0 1.9288219297119045 1.8425975761365927E-5 64-65 1.8425975761365927E-5 0.0 0.0 2.1869698501286408 1.8425975761365927E-5 66-67 1.8425975761365927E-5 0.0 0.0 2.4781371191097454 1.8425975761365927E-5 68-69 1.8425975761365927E-5 0.0 0.0 2.808818893111099 1.8425975761365927E-5 70-71 1.8425975761365927E-5 0.0 0.0 3.173404462513366 1.8425975761365927E-5 72-73 1.8425975761365927E-5 0.0 0.0 3.5496260356089353 3.685195152273185E-5 74-75 1.8425975761365927E-5 0.0 0.0 3.9189655067576346 3.685195152273185E-5 76-77 1.8425975761365927E-5 0.0 0.0 4.350142552561477 3.685195152273185E-5 78-79 1.8425975761365927E-5 0.0 0.0 4.812910933808183 3.685195152273185E-5 80-81 1.8425975761365927E-5 0.0 0.0 5.309168526001171 3.685195152273185E-5 82-83 1.8425975761365927E-5 0.0 0.0 5.830476232241736 3.685195152273185E-5 84-85 1.8425975761365927E-5 0.0 0.0 6.331902310635787 3.685195152273185E-5 86-87 1.8425975761365927E-5 0.0 0.0 6.869406449570592 3.685195152273185E-5 88 1.8425975761365927E-5 0.0 0.0 7.300813820071452 3.685195152273185E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 6665 0.0 43.682392 94 GTACTAG 6155 0.0 36.36063 1 CTGTGCG 8810 0.0 35.149395 9 TAGTACT 9275 0.0 33.89788 4 ACTGTGC 9545 0.0 33.082756 8 CAACGCA 8895 0.0 33.01919 5 GCATCGT 6420 0.0 32.64436 8 TCTAACG 3210 0.0 32.502766 2 TAGGCAT 7960 0.0 32.23383 5 CTAACGC 3230 0.0 32.155415 3 GTATCAA 10590 0.0 31.877256 1 GTACTGT 9950 0.0 31.405874 6 CGCCTAC 3235 0.0 31.375584 7 TAACGCC 3340 0.0 31.236824 4 ATCAACG 9620 0.0 30.532988 3 CTAGTAC 10785 0.0 30.415829 3 TATTCTC 2975 0.0 30.3282 5 TACTGTG 10865 0.0 30.318005 7 CAGTACG 1060 0.0 29.701426 9 CTAGGCA 8980 0.0 29.35938 4 >>END_MODULE