FastQCFastQC Report
Mon 16 Jan 2017
ERR1146645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427121
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131790.24283593455904154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111850.20609453889087787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67860.12503867151662917No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC63400.042.5039294
GGTATCA105300.041.947781
GTATCAA184400.039.2344131
GTACTAG63700.034.929591
CTGTGCG85000.033.843879
CAACGCA207200.033.1629455
GTGGTAT24600.033.0813181
TAGTACT89100.033.047334
ATCAACG210000.032.769123
ACTGTGC92450.031.9774048
TAGGCAT81150.030.9277425
GTACTGT95550.030.8395716
TATCAAC236350.030.6902582
TACTGTG104850.030.210857
AACGCAG243050.029.4686896
CTAGTAC105450.028.6816483
TCTAACG30100.028.5993652
CTAGGCA91150.028.278974
CTAACGC30800.028.1000333
TAACGCC31500.027.624914
Produced by FastQC (version 0.11.5)