FastQCFastQC Report
Mon 16 Jan 2017
ERR1146644_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146644_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3754843
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC42500.042.351294
GTACTAG46400.035.6674271
TAGTACT61800.031.55934
CTGTGCG61450.031.1999579
TAGGCAT57900.031.0855875
GCATCGT48650.030.715678
CGTTAAC8300.030.5888251
ACTGTGC66900.030.0630728
GTACTGT66350.029.249936
CTAACGC25650.028.949263
CGCCTAC26500.028.5493147
CTAGGCA65000.028.4872594
TCTAACG26050.028.3243332
TACTGTG73950.028.1501877
CAACGCA85950.027.7751675
CTAGTAC75400.027.6744753
TACCCCG2400.027.4129435
TAACGCC27350.027.321694
GTATCAA98950.027.0360681
TATTCTC17850.026.8534935
Produced by FastQC (version 0.11.5)